Table S4 Predicted functions of probe sets differentially expressed in six Lr1 wheat transgenic lines at 6, 24 and 6 and 24 HPI compared to prior to inoculation (0) that encode enzymes of various pathways represented in Figure 1a (up-regulated) and Figure 1b (down-regulated). The values with minus (-) sign indicate down-regulated transcripts
Gene ID / FoldChangea / P-valueb / Time / E-value / Predicted functionc
TaAffx.108909.1.S1_at / 4.6 / 0.00008 / R6-24 / 5E-20 / POP2 (pollen-pistil incompatibility 2); 4-aminobutyrate transaminase
Ta.278.1.S1_at / 6.5 / 0.00000 / R24 / 1E-83 / PR1 (pathogenesis-related GENE 1)
Ta.217.1.S1_x_at / 2.4 / 0.00002 / R6-24 / 0 / HSP81-2 (early-responsive to dehydration 8); ATP binding
Ta.24630.2.S1_x_at / 6.5 / 0.00001 / R6 / 8E-10 / Splicing factor PWI domain-containing protein
Ta.21342.1.S1_x_at / 6.3 / 0.00000 / R24 / 1E-175 / ATHCHIB (basic chitinase)
Ta.9226.1.S1_at / 5.8 / 0.00003 / R24 / 5E-82 / PR4 (pathogenesis-related 4)
Ta.1174.1.S1_x_at / 5.9 / 0.00000 / R24 / 1E-129 / BGL2 (pathogenesis-related protein 2); glucan 1,3-beta-glucosidase
Ta.22082.1.S1_at / 2.1 / 0.00007 / R6 / 5E-54 / MLS (malatesynthase)
TaAffx.25966.1.S1_x_at / 2.0 / 0.00005 / R24 / 3E-16 / CAT2 (catalase 2)
TaAffx.104885.2.S1_at / 3.2 / 0.00002 / R6 / 4E-48 / FMO1 (flavin-dependent monooxygenase 1)
Ta.5507.1.S1_at / 2.7 / 0.00001 / R6 / 4E-28 / Wound-responsive family protein
Ta.7752.1.S1_x_at / 2.2 / 0.00001 / R24 / 1E-136 / RKL1 (Receptor-like kinase 1); ATP binding serine/threoninekinase
Ta.972.2.S1_a_at / 1.4 / 0.00001 / R6 / 0 / ATPANK2 (pantothenatekinase 2)
Ta.6301.2.S1_a_at / 2.3 / 0.00003 / R6 / 0 / CPK5 (calmodulin-domain protein kinase 5)
TaAffx.99198.1.S1_at / 2.1 / 0.00010 / R24 / 6E-86 / HDA08 (histonedeacetylase 8)
Ta.1767.1.A1_at / 2.6 / 0.00001 / R24 / 0.0000002 / Stripe rust resistant protein Yr10
Ta.30657.1.S1_at / 1.9 / 0.00003 / R24 / 1E-111 / RPM1 (resistance to P. syringaepvmaculicola 1); protein binding
Ta.13264.1.A1_at / 3.0 / 0.00000 / R6 / 4E-43 / MAPKKK5 (Mitogen-activated protein kinasekinasekinase 5)
Ta.27258.1.S1_at / 2.9 / 0.00000 / R24 / 6E-59 / SERK2 (somatic embryogenesis receptor-like kinase 2)
Ta.27477.1.S1_at / 4.5 / 0.00000 / R24 / 7E-26 / GLP4 (germin-like protein 4); manganese ion binding
Ta.6322.2.S1_a_at / 2.0 / 0.00006 / R6 / 0 / COI1 (coronatine insensitive 1); ubiquitin-protein ligase
Ta.2583.2.S1_x_at / 1.6 / 0.00009 / R6 / 7E-47 / 3-hydroxyacyl-CoA dehydrogenase
Ta.20061.1.S1_at / 1.7 / 0.00007 / R24 / 7E-22 / RIN4 (RPM1 interacting protein 4); protein binding
Ta.6517.1.S1_x_at / 2.6 / 0.00000 / R6 / 2E-83 / Protein phosphatase 2C family protein
Ta.280.1.S1_s_at / 3.0 / 0.00004 / R6 / 0 / MLO10 (mildew resistance locus O 10); calmodulin binding
Ta.280.2.S1_x_at / 2.2 / 0.00009 / R6 / 0 / MLO2 (mildew resistance locus O 2); calmodulin binding
Ta.13724.1.S1_at / 2.0 / 0.00001 / R24 / 3E-25 / AREB3 (ABA-responsive element binding protein 3)/transcription activator
Ta.2583.2.S1_at / 0.9 / 0.00002 / R24 / 7E-47 / MFP2 (multifunctional protein); Enoyl-CoAhydratase
Ta.27259.1.S1_at / 5.7 / 0.00007 / R6 / 1E-105 / ATSDAT/ATTDT (tonoplastdicarboxylate transporter); malatetransmembrane transporter
Ta.2107.1.S1_s_at / 5.5 / 0.00004 / R6 / 0 / ALDH7B4 (aldehydedehydrogenase 7B4); 3-chloroallyl aldehydedehydrogenase
TaAffx.79139.1.S1_at / 8.1 / 0.00001 / R24 / 6E-12 / Isocitratelyase
Ta.9320.1.S1_x_at / 6.2 / 0.00000 / R24 / 1E-126 / Caffeoyl-CoA 3-O-methyltransferase
Ta.6649.1.S1_at / 6.2 / 0.00000 / R24 / 0.000002 / RNA recognition motif (RRM)-containing protein
TaAffx.85427.2.S1_x_at / 5.3 / 0.00000 / R6 / 3E-25 / Pathogen inducible transcription factor ERF3
Ta.3840.1.S1_at / 3.9 / 0.00000 / R24 / 2E-98 / 5'-AMP-activated protein kinase beta-2 subunit
Ta.8614.1.S1_at / 3.5 / 0.00002 / R24 / 1E-118 / WRKY70 (WRKY DNA-binding protein 70); transcription factor
Ta.29531.1.S1_x_at / 5.9 / 0.00003 / R6 / 5E-12 / Peroxidase 32 (PER32)
Ta.62.1.S1_x_at / 7.0 / 0.00007 / R24 / 3E-31 / Pathogenesis-Related protein
Ta.13337.1.S1_at / 8.5 / 0.00000 / R24 / 2E-38 / Xyloglucan:xyloglucosyltransferase
TaAffx.39568.1.S1_at / 6.8 / 0.00003 / R24 / 1E-37 / Peroxidase
TaAffx.77051.1.A1_s_at / -2.1 / 0.000008 / R6 / 9E-50 / MRH1 (morphogenesis of root hair 1); ATP binding/protein serine/threoninekinase
Ta.1981.3.S1_at / -2.0 / 0.000000 / R6 / 3E-35 / DNA binding/ligand-dependent nuclear receptor
Ta.30763.1.S1_x_at / -2.8 / 0.000034 / R24 / 4E-94 / ATEXPB4 (Arabidopsis thalianaexpansin B4)
Ta.18362.1.A1_at / -2.5 / 0.000079 / R24 / 1E-64 / ELIP1 (early light-inducable protein); chlorophyll binding
TaAffx.56132.2.S1_at / -2.7 / 0.000000 / R24 / 1E-15 / HAK5 (High affinity K+ transporter 5)
Ta.11136.1.A1_at / -4.0 / 0.000003 / R24 / 5E-99 / ANTR2 (anion transporter 2)
TaAffx.52905.1.S1_x_at / -2.6 / 0.000059 / R24 / 6E-20 / PRK (phosphoribulokinase); ATP binding/phosphoribulokinase/protein binding
Ta.4878.1.S1_at / -5.0 / 0.000074 / R24 / 0 / AtGH9A1 (Arabidopsis thalianaglycosylhydrolase 9A1)
Ta.23069.1.S1_at / -4.2 / 0.000032 / R6 / 2E-66 / SHY2 (short hypocotyl 2); transcription factor
Ta.5948.1.S1_at / -3.5 / 0.000003 / R6 / 9E-46 / PPOX (protoporphyrinogenoxidase)
Ta.28700.1.S1_at / -7.2 / 0.000053 / R6 / 0 / ATCHS/CHS/TT4 (chalconesynthase); naringenin-chalconesynthase
TaAffx.69760.2.S1_at / -6.0 / 0.000000 / R24 / 2E-11 / C/VIF2 (cell wall/vacuolar inhibitor of fructosidase 2); pectinesterase inhibitor
Ta.8718.2.S1_at / -5.7 / 0.000012 / R6 / 2E-54 / ATP synthase (delta chain)
Ta.9542.2.S1_x_at / -4.6 / 0.000000 / R6 / 3E-78 / CIPK2 (CBL-interacting protein kinase 2)
Ta.17503.1.A1_at / -1.3 / 0.000073 / R6-24 / 3E-11 / SPA1 (suppressor of phya-105 1); signal transducer
TaAffx.61784.1.S1_at / -2.2 / 0.000013 / R24 / 1E-107 / VIT1 (vacuolar iron transporter 1)
Ta.3970.1.S1_at / -2.1 / 0.000002 / R6 / 5E-13 / STP1 (sugar transporter 1)/sugar:hydrogen ion symporter
Ta.5468.1.A1_at / -2.7 / 0.000001 / R24 / 7E-34 / GLR2 (glutamate receptor 2)
Ta.4449.1.A1_at / -1.6 / 0.000050 / R24 / 8E-40 / APE2 (acclimation of photosynthesis to environment)
Ta.946.1.S1_at / -2.5 / 0.000018 / R6 / 1E-163 / TMT1 (tonoplast monosaccharide transporter 1)
Ta.7930.1.S1_at / -3.8 / 0.000001 / R24 / 6E-23 / SEP1 (stress enhanced protein 1)
Ta.485.1.A1_at / -3.0 / 0.000040 / R24 / 1E-129 / LOX2 (lipoxygenase 2)
Ta.10399.1.S1_at / -3.0 / 0.000036 / R24 / 1E-108 / Shikimatekinase family protein
Ta.27312.1.S1_x_at / -2.1 / 0.000090 / R24 / 0 / ATAMT1;2 (ammonium transporter 1;2)
Ta.1601.1.S1_at / -2.6 / 0.000011 / R6 / 0 / EMB1075 (embryo defective 1075); carboxy-lyase
Ta.862.1.A1_at / -3.9 / 0.000023 / R24 / 2E-19 / CUE1 (CAB underexpressed 1); antiporter/triose-phosphate transmembrane transporter
TaAffx.65079.1.A1_x_at / -3.0 / 0.000000 / R24 / 2E-20 / IAA12 (auxin-induced protein 12); transcription factor
Ta.10310.3.S1_at / -3.2 / 0.000070 / R24 / 1E-36 / Proline-rich extensin-like family protein
Ta.30449.1.A1_at / -2.4 / 0.000005 / R24 / 1E-116 / SFR2 (sensitive to freezing 2); hydrolase, hydrolyzing O-glycosyl compounds
Ta.4023.1.S1_at / -2.5 / 0.000006 / R24 / 0 / PKP3 (plastidialpyruvatekinase 3)
Ta.12666.1.S1_at / -6.2 / 0.000001 / R24 / 2E-16 / SAG21 (senescence-associated gene 21)
Ta.4001.1.S1_at / -5.2 / 0.000015 / R24 / 0 / PHT2;1 (phosphate transporter 2;1)
Ta.3867.3.S1_a_at / -5.1 / 0.000027 / R6 / 0 / VTC5; galactose-1-phosphate guanylyltransferase (GDP)
Ta.19092.1.S1_x_at / -1.7 / 0.000054 / R24 / 0 / SIR (sulfite reductase) (ferredoxin)
Ta.5600.2.S1_a_at / -2.5 / 0.000051 / R24 / 1E-64 / STO (salt tolerance); transcription factor/zinc ion binding
Ta.1158.1.S1_a_at / -2.5 / 0.000017 / R24 / 0 / F2KP (fructose-2,6-bisphosphatase)
Ta.1166.2.S1_at / -2.8 / 0.000006 / R24 / 1E-121 / Fructose-1,6-bisphosphatase/FBPase
Ta.1464.2.S1_a_at / -3.0 / 0.000012 / R6 / 1E-170 / ZIFL2 (zinc induced facilitator-like 2)/sugar:hydrogen ion symporter
Ta.28670.1.S1_at / -2.4 / 0.000057 / R24 / 1E-139 / APG1 (albino or pale green mutant 1); methyltransferase
a Fold-change value of ≥ 1.5; minus sign indicate down-regulation
bP-value threshold of ≤ 0.001
c Based on the best BlastX hit with E-value threshold of ≤ 10-6 against the HarvEST database