A. Demographic and Personal Information

A. Demographic and Personal Information

Curriculum Vitae

Amanda M. Brown, Ph.D.

A. Demographic and Personal Information

Current Appointment:

Assistant Professor June 2010-Present

Department of Neurology, School of Medicine

Personal Data:

Department of Neurology

600 North Wolfe Street, Meyer 6-181

Baltimore, MD 21287-7131

(Office) 410-614-2429

(Fax) 410-502-6737

B. EDUCATION & TRAINING

1985-1989 BS University of California RiversideBiochemistry Riverside, CA

  • Summer Research Experience 1988 Carnegie Mellon University
  • Summer Research Experience 1987 University of California Davis

1996-1989 Ph.D. Albert Einstein College of Medicine Microbiology/Immunology Bronx, NY

  • Thesis: Isolation and characterization of Sec A, the central component of the general protein secretion pathway in mycobacteria. Advisors: Drs. William R. Jacobs, Jr., Professor and Investigator of The Howard Hughes Medical Institute and Barry Bloom, currently Dean of Harvard School of Public Health.
  • June 1991 Cold Spring Harbor Course on Advanced Bacterial Genetics.

1999-1996 Postdoctoral Fellow Aaron Diamond AIDS Research Center, New York, NY

Advisor: Dr. Cecilia Cheng-Mayer, Professor and Staff Scientist

  • Identified host cell factors found in primary T-cells and macrophages that are downstream signal transducers for HIV-1 Nef.
  • Attended the second annual conference on Differential Display at Cold Spring Harbor Laboratory in 1996.

1999-2003 (See below)

2/2004-9/2004 Postdoctoral Fellow Johns Hopkins School of Medicine, Baltimore, MD

Advisor: Dr. Suzanne Gartner, Associate Professor, Department of Neurology

C. PROFESSIONAL EXPERIENCE

07/1999-12/2003 Research Scientist Aaron Diamond AIDS Research Center, New York, NY

09/2004-02/2007 Research Associate Johns Hopkins School of Medicine, Baltimore, MD

03/2007-06/2010 Instructor Johns Hopkins School of Medicine, Baltimore, MD

06/2010-PresentAssistant ProfessorJohns Hopkins School of Medicine, Baltimore, MD
D. RESEARCH ACTIVITIES

Publications

1. McAdam, R., Weisbrod, T., Martin, J., Scuderi, J., Brown, A., Cirrilo, J., Bloom, B., and W. R. Jacobs Jr. In vivo growth characteristics of leucine and methionine auxotrophic mutants of Mycobacterium bovis BCG generated by transposon mutagenesis. Infection and Immunity. 1995;63:1004-1012.

2. Bange, F-C., Brown, A., and W. R. Jacobs Jr. Leucine auxotrophy restricts growth of Mycobacterium bovis BCG in macrophages. Infection and Immunity. 1996;64:1794-1799.

3. Cheng-Mayer, C., Brown, A., Harouse, J., Luciw, P. A., and A. J. Mayer. Selection for neutralization resistance of the simian/human immunodeficiency virus SHIVSF33A variant in vivo by virtue of sequence changes in the extracellular envelope glycoprotein that modify N-linked glycosylation. J. Virol. 1999;73:5294-5300.

4. Brown, A., Wang, X., Sawai, E. and C. Cheng-Mayer. Activation of the PAK-related kinase by human immunodeficiency type 1 Nef in primary human peripheral blood lymphocytes and macrophages leads to phosphorylation of a PIX-p95 complex. J. Virol. 1999;73:9899-9907

5. Braunstein, M., Brown, A., Kurtz, S. and W. R. Jacobs, Jr. Two nonredundant SecA homologues function in mycobacteria. J. Bacteriol. 2001;183:6979-6990.

6. Brown, A., Moghaddam, S., Kawano, T. and C. Cheng-Mayer. Multiple human immunodeficiency type 1 Nef functions are required for efficient replication in primary human macrophages. J. Gen. Virol. 2004;85:1451-1461.

7. Brown, A., Gartner, S., Kawano, T., Benoit, N. and C. Cheng-Mayer. HLA-A2 down-regulation on primary human macrophages infected with a M-tropic EGFP-tagged HIV-1 reporter virus. J. of Leukocyte Biol. 2005;78:675-685.

8. Brown, A., Zhang, H., Lopez, P., Pardo, C. and S. Gartner. In vitro modeling of the HIV-macrophage reservoir. J. of Leukocyte Biol. 2006;80:1127-1135.

9. Brown, A., Use of a macrophage-tropic GFP-tagged human immunodeficiency virus type 1 (HIV-1) to study viral reservoirs. Methods in Mol. Biol 2009;515:165-175.

10. Grab, D, Garcia-Garcia, J. C., Nikolskaia, O V., Kim, Y. V., Brown, A., Pardo, C. A., Zhang, Y., Becker, K. G., Wilson, B. A., de A Lima, A P., Scharfstein, J., and J. S. Dumler. Protease activated receptor signaling is required for African typanosome traversal of human brain microvascular endothelial cells. PLOS Neglected Tropical Diseases 2009: 3(7):e479.

11. Hammoud DA, Endres C.J., Hammond E., Uzuner O., Brown A., Nath A., Kaplin A.I., Pomper M.G. Imaging serotonergic transmission with [(11)C]DASB-PET in depressed and non-depressed patients infected with HIV. Neuroimage 2009; 49:2588-2595.

12. Brown, A., Shiramizu, B., Nath, A., Valerie Wojna. 2011. Translational research in neuroAIDS: A neuroimmune pharmacology related CME course, J. Neuroimmun. Pharmacol. 6:80-88;Epub 2010 May 25.

13. Brown, A., Islam, T., Adams, R., Nerle, S., Kamara, M., Eger, C., Marder, K., Cohen, B., Schifitto, G., McArthur, J., Sacktor, N., and C. Pardo. 2011. Osteopontin enhances HIV-1 replication and is increased in the brain and cerebrospinal fluid of HIV-infected individuals. J. Neurovirology 17:382-392.

14. Brown, A., Sacktor, N., Marder, K., Cohen, B., Schifitto, G., Skolasky, R., Creighton, J., Guo, L and J. McArthur. 2012. CCL3L1 gene copy number in individuals with and without HIV-associated neurocognitive disorder. Current Biomarker Findings 2012; 2: 1-6.

C. RESEARCH FUNDING

01/31/10-12/30/10; Osteopontin: An inflammatory mediator in neurodegenerative diseases; Margaret Q. Landenberger Research Foundation; $110,000; Principal Investigator: Amanda Brown

01/11/12-12/31/13 NIMH R21MH095646-01 (PI: Brown) The role of osteopontin in HIV-associated neurocognitive disorder: National Institutes of Mental Health 1; $275,000 (direct costs)

04/30/12-04/30/15 NIMH R25MH080661-05 (PI: McArthur; Co-PI: Brown)

Translational Research in NeuroAIDS and Mental Health

Train researchers in issues that face HIV infected populations of racial and ethnic minorities.

$231, 821 (direct costs in year 2011)

D. EDUCATIONAL ACTIVITIES

Educational publications

Brown, A. (Editor), Burton, G., Craigo, J., Leroux, C., Luciw, P., Montelaro, R., North, T., Shacklett, B., and Margolis, D. “Latent Infection by HIV-1 and Related Lentiviruses: New Approaches and Treatment Challenges”. Kerala, India. Transworld Network (2007).

Teaching-Web-based Course

August-December 2007-Present; Translational Research in NeuroAIDS and Mental Health; Course co-director; web-based, journal club held by webinar format. Funded by grant NIMH R25 MH080661.

Teaching/Mentoring

October 2005-Present Instructor for tutorial ME.990.331 entitled Laboratory investigations of HIV neuropathogenesis” now, “Molecular genetic studies in HIV-macrophage interactions.

E. Organizational Activities

Department of Neurology Johns Hopkins Internship in Brain Sciences Program

Program Director 2008-Present

Journal peer review:

Journal of Neuroscience 2005-Present

Journal of Neurovirology 2010-present

Research & Reviews-A Journal of Neuroscience 2011-Section Editor

Professional societies

American Association for the Advancement of Science 1996-present

Society for Leukocyte Biology 2005-present

Women and Diversity Task Force 2010-present

American Society for Cell Biology 2010

Oral presentations

Brown, A., Sawai, E. and C. Cheng-Mayer. 1999. HIV-1 Nef activates PAK kinase in infected human primary macrophages. The 6th Conference on Retroviruses and Opportunistic Infections, Chicago, IL.

Brown, A., Lopez, P., Zhang, H., and Gartner, S. 2005. In vitro modeling of the HIV-macrophage reservoir. 6th International Workshop on HIV, Cells of Macrophage/Dendritic Lineage and Other Reservoirs, Varenna, Italy.

Brown, A. May 2009. Research tools to isolate and study HIV-infected macrophage subpopulations. The Second Annual Symposium on Virology and Laser Microdissection. Integrated Research Facility, Division of Clinical Research/NIAID/NIH, Bethesda, Maryland.

Poster presentations

1. Brown, A.,Wang, X., Sawai, E. and C. Cheng-Mayer. 1999. Activation of the PAK-Related Kinase by HIV-1 Nef in Primary Human Peripheral Blood Lymphocytes and Macrophages leads to Phosphorylation of a PIX-p95 Complex. The 8th International Symposium of the Society of Chinese Bioscientists in America (SCBA), Hong Kong, China

2. Brown, A., Kawano, T. and C. Cheng-Mayer. 2000. Construction of a recombinant wild-type macrophage-tropic reporter virus for use in primary cellls. Cold Spring Harbor, NY.

3. Brown, A., Kawano, T. and C. Cheng-Mayer. 2003. Single-cell analysis of HIV-1 infected primary human macrophages. The 10th Conference on Retroviruses and Opportunistic Infections, Boston, MA.

4. Kumar, S., Brown, A., Liu, Y., and S. Gartner. 2005. Detection of a shared HIV-protease-RT deletion in patient plasma and cells: a role for ARV-mediated selection and viral complementation. International Meeting of the Institute of Human Virology, Baltimore, MD.

5. Brown, A., Lopez, P., Zhang, H., and S. Gartner. 2005. In vitro modeling of the HIV-macrophage reservoir. 6th International Workshop on HIV, Cells of Macrophage/Dendritic Lineage and Other Reservoirs, Varenna, Italy.

6. Brown, A., Kamara, M., Kosel, A and Gartner, S. 2006. Isolation of messages selectively expressed in HIV-infected primary human macrophages. Genomic perspectives to host pathogen interactions. Hinxton, UK.

7. Brown, A., Skolasky, R., Sacktor, N, Marder, K, Cohen, B, Schifitto, G., Creighton, J, Guo, L and J. McArthur. 2009. CCL3L1 gene copy number in the Northeast AIDS dementia (NEAD) cohort and the National NeuroAIDS Tissue Consortium (NNTC). The 16th Conference on Retoroviruses and Opportunistic Infections, Montreal, Canada.

8. Grab, D, Garcia-Garcia, J. C., Nikolskaia, O V., Kim, Y. V., Brown, A., Pardo, C. A., Kim, Y. V., Zhang, Y., Becker, K. G., Wilson, B. A., de A Lima, A P., Scharfstein, J., and J. S. Dumler. 2009. African trypanosome traversal of the human blood-brain barrier: A role for protease activated receptor signaling? 36th Annual Meeting on Basic Research on Chagas Disease, Rio De Janeiro, Brazil.

9. Nyquist, P., An, P., Brown, A., Kirk, G., Hoke, A., Winkler, C., and J. C. McArthur. 2009. Monocyte chemoattractant factor 1 (MCP-1) receptor (CCR2) single nucleotide polymorphism is associated with increased risk of HIV-associated peripheral neuropathy. American Neurological Association (ANA).

10. Adams, R., Kamara, M., Islam, T., Eger, C., and A. Brown. 2010. The role of osteopontin in HIV neuropathogenesis: in vitro and ex vivo analyses. 10th International Society for Neurovirology Conference, Milan, Italy.

11. Weaver, C., Huang, S., and A. Brown. 2010. Relocalization of HIV-Gag+CD81+ virus containing compartments to the plasma membrane of primary human macrophages. 50th American Society for Cell Biology Conference, Philadelphia, PA and The Society for Leucocyte Biology, Vancouver British Columbia Annual Meeting.

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