A, Average Number of Codons Found Per 1,000 Codons

Supplementary Table 2. Codon usage of Bacillusspp.(B. cereus ATCC 10987[gbbct]: 5869, B. thuringiensis [gbbct]: 496, B. subtilis [gbbct]: 2529, B. licheniformis [gbbct]: 202) and BCP8-2 ( found in BCP8-2 are underlined. Codon usage bias (phage/host ratio) was calculated by the formula(average number of codons found in phage / average number of codons found in bacteria per 1,000 codons).MCP, major capsid protein (ORF20); TSH, tail sheath protein (ORF13).

Amino
Acid / Codon / B. cereus / B. thuringiensis / B. subtilis / B. licheniformis / BCP8-2 (Number of codons)
Totala / Phage/hostb / Total / Phage/host / Total / Phage/host / Total / Phage/host / Total
(53024) / Structuralc
(13060) / MCP
(484) / TSH
(569)
Gly / GGG / 9.36 / 0.44 / 9.78 / 0.42 / 11.15 / 0.37 / 12.2 / 0.34 / 4.10 / 1.23 / 0 / 0
GGA / 24.37 / 0.40 / 24.75 / 0.39 / 21.76 / 0.45 / 23.81 / 0.41 / 9.70 / 3.68 / 0 / 0
GGT / 24.22 / 0.49 / 21.74 / 0.54 / 12.96 / 0.91 / 10.17 / 1.16 / 11.80 / 4.06 / 0 / 0
GGC / 8.49 / 0.39 / 6.56 / 0.50 / 23.26 / 0.14 / 25.8 / 0.13 / 3.30 / 5.13 / 0 / 3.51
Glu / GAG / 18.65 / 0.45 / 17.76 / 0.47 / 22.60 / 0.37 / 22.06 / 0.38 / 8.40 / 1.23 / 0 / 0
GAA / 56.56 / 0.29 / 51.07 / 0.32 / 48.09 / 0.34 / 49.96 / 0.33 / 16.40 / 25.34 / 33.06 / 31.63
Asp / GAT / 37.29 / 0.43 / 43.13 / 0.37 / 33.24 / 0.48 / 31.76 / 0.51 / 16.10 / 28.02 / 47.52 / 38.66
GAC / 8.72 / 0.87 / 9.18 / 0.83 / 18.99 / 0.40 / 24.24 / 0.31 / 7.60 / 4.52 / 2.07 / 1.76
Val / GTG / 10.91 / 1.06 / 11.13 / 1.04 / 17.30 / 0.67 / 15.27 / 0.76 / 11.60 / 5.97 / 8.26 / 5.27
GTA / 30.23 / 0.78 / 26.85 / 0.88 / 13.05 / 1.82 / 11.01 / 2.15 / 23.70 / 26.72 / 24.79 / 35.15
GTT / 25.03 / 0.96 / 19.84 / 1.21 / 18.63 / 1.29 / 16.17 / 1.49 / 24.10 / 40.81 / 55.79 / 52.72
GTC / 5.73 / 2.44 / 5.58 / 2.51 / 17.25 / 0.81 / 21.68 / 0.65 / 14.00 / 26.72 / 30.99 / 42.18
Ala / GCG / 12.85 / 0.27 / 9.11 / 0.38 / 19.84 / 0.18 / 21.16 / 0.17 / 3.50 / 1.23 / 2.07 / 0
GCA / 28.65 / 0.32 / 24.46 / 0.37 / 21.10 / 0.43 / 18.78 / 0.48 / 9.10 / 1.45 / 0 / 0
GCT / 20.45 / 0.56 / 18.83 / 0.61 / 18.58 / 0.61 / 15.37 / 0.74 / 11.40 / 5.13 / 6.20 / 3.51
GCC / 4.02 / 0.95 / 4.71 / 0.81 / 16.47 / 0.23 / 19.6 / 0.19 / 3.80 / 2.45 / 2.07 / 0
Arg / AGG / 2.67 / 3.82 / 4.84 / 2.11 / 4.10 / 2.49 / 6.78 / 1.50 / 10.20 / 18.38 / 14.46 / 21.09
AGA / 9.69 / 1.65 / 17.41 / 0.92 / 10.50 / 1.52 / 11.68 / 1.37 / 16.00 / 21.29 / 33.06 / 31.63
Ser / AGT / 14.36 / 1.27 / 19.58 / 0.93 / 6.84 / 2.68 / 4.52 / 4.05 / 18.30 / 22.21 / 33.06 / 43.94
AGC / 5.71 / 1.91 / 6.28 / 1.74 / 14.43 / 0.76 / 18.55 / 0.59 / 10.90 / 26.65 / 53.72 / 38.66
Lys / AAG / 18.53 / 0.86 / 14.33 / 1.11 / 20.84 / 0.76 / 21.45 / 0.74 / 15.90 / 11.87 / 8.26 / 12.30
AAA / 56.31 / 0.30 / 46.52 / 0.37 / 48.38 / 0.35 / 48.81 / 0.35 / 17.10 / 10.03 / 6.20 / 1.76
Asn / AAT / 33.27 / 0.65 / 55.15 / 0.39 / 22.93 / 0.95 / 21.84 / 0.99 / 21.70 / 27.03 / 20.66 / 19.33
AAC / 13.02 / 1.44 / 15.97 / 1.17 / 17.81 / 1.05 / 23.67 / 0.79 / 18.70 / 21.67 / 33.06 / 28.12
Met / ATG / 27.68 / 0.49 / 18.25 / 0.75 / 26.27 / 0.52 / 23.4 / 0.59 / 13.70 / 5.59 / 2.07 / 0
Ile / ATA / 17.30 / 1.35 / 18.37 / 1.27 / 9.76 / 2.40 / 6.8 / 3.44 / 23.40 / 13.71 / 4.13 / 3.51
ATT / 49.81 / 0.42 / 37.70 / 0.56 / 36.16 / 0.58 / 25.64 / 0.82 / 21.10 / 19.37 / 4.13 / 8.79
ATC / 13.07 / 1.36 / 10.02 / 1.78 / 27.18 / 0.65 / 33.21 / 0.54 / 17.80 / 34.38 / 26.86 / 19.33
Thr / ACG / 13.21 / 1.04 / 10.33 / 1.33 / 14.85 / 0.92 / 19.34 / 0.71 / 13.70 / 17.08 / 26.86 / 35.15
ACA / 27.17 / 0.53 / 30.29 / 0.48 / 21.63 / 0.67 / 18.25 / 0.79 / 14.50 / 1.84 / 0 / 0
ACT / 12.30 / 1.63 / 20.21 / 0.99 / 8.68 / 2.30 / 6.25 / 3.20 / 20.00 / 15.31 / 4.13 / 10.54
ACC / 2.78 / 5.97 / 5.93 / 2.80 / 9.00 / 1.84 / 9.88 / 1.68 / 16.60 / 42.27 / 39.26 / 49.21

Supplementary Table 1.Continued.

Amino
Acid / Codon / B. cereus / B. thuringiensis / B. subtilis / B. licheniformis / BCP8-2 (Number of codons)c
Totala / Phage/hostb / Total / Phage/host / Total / Phage/host / Total / Phage/host / Total(53024) / Structural(13060) / MCP
(484) / TSH
(569)
Trp / TGG / 10.22 / 0.82 / 11.69 / 0.72 / 10.75 / 0.78 / 13.07 / 0.64 / 8.40 / 12.56 / 24.79 / 14.06
Stop / TGA / 0.63 / 23.97 / 0.24 / 62.92 / 0.76 / 19.87 / 0.56 / 26.96 / 15.10 / 14.01 / 10.33 / 14.06
Cys / TGT / 6.44 / 3.96 / 6.90 / 3.70 / 3.60 / 7.08 / 2.75 / 9.27 / 25.50 / 34.46 / 26.86 / 29.88
TGC / 2.29 / 5.85 / 2.81 / 4.77 / 4.26 / 3.15 / 4.86 / 2.76 / 13.40 / 30.86 / 39.26 / 38.66
Stop / TAG / 0.60 / 31.33 / 0.54 / 34.81 / 0.47 / 40.00 / 0.40 / 47.00 / 18.80 / 18.91 / 18.60 / 24.60
Stop / TAA / 2.56 / 10.27 / 1.57 / 16.75 / 1.92 / 13.70 / 1.49 / 17.65 / 26.30 / 29.79 / 49.59 / 40.42
Tyr / TAT / 28.06 / 0.80 / 37.22 / 0.60 / 23.26 / 0.96 / 24.93 / 0.90 / 22.40 / 7.58 / 0 / 0
TAC / 9.43 / 2.80 / 8.59 / 3.07 / 12.62 / 2.09 / 15.77 / 1.67 / 26.40 / 36.60 / 45.45 / 50.97
Leu / TTG / 9.89 / 2.22 / 10.27 / 2.14 / 15.84 / 1.39 / 17.91 / 1.23 / 22.00 / 30.47 / 61.98 / 31.63
TTA / 48.88 / 0.51 / 42.89 / 0.58 / 19.83 / 1.25 / 12.75 / 1.95 / 24.80 / 2.07 / 0 / 1.76
Phe / TTT / 32.92 / 0.99 / 32.34 / 1.01 / 29.98 / 1.09 / 25.17 / 1.30 / 32.60 / 42.19 / 43.39 / 33.39
TTC / 14.13 / 1.98 / 10.34 / 2.71 / 14.32 / 1.96 / 14.85 / 1.89 / 28.00 / 35.83 / 28.93 / 24.60
Ser / TCG / 4.51 / 2.75 / 4.87 / 2.55 / 6.46 / 1.92 / 9.58 / 1.29 / 12.40 / 6.20 / 0 / 5.27
TCA / 14.46 / 1.17 / 15.93 / 1.06 / 14.56 / 1.16 / 12.67 / 1.33 / 16.90 / 0.08 / 0 / 0
TCT / 15.29 / 1.69 / 18.93 / 1.36 / 12.66 / 2.04 / 9.00 / 2.87 / 25.80 / 14.09 / 2.07 / 7.03
TCC / 3.35 / 4.90 / 5.54 / 2.96 / 8.30 / 1.98 / 9.70 / 1.69 / 16.40 / 24.50 / 10.33 / 31.63
Arg / CGG / 1.46 / 3.77 / 1.58 / 3.48 / 6.95 / 0.79 / 8.60 / 0.64 / 5.50 / 6.13 / 2.07 / 0
CGA / 5.53 / 1.84 / 6.78 / 1.50 / 4.34 / 2.35 / 3.92 / 2.60 / 10.20 / 5.44 / 2.07 / 1.76
CGT / 13.76 / 1.16 / 9.70 / 1.64 / 7.24 / 2.20 / 4.89 / 3.25 / 15.90 / 14.24 / 12.40 / 12.30
CGC / 4.66 / 0.94 / 2.77 / 1.59 / 8.24 / 0.53 / 9.93 / 0.44 / 4.40 / 8.04 / 8.26 / 8.79
Gln / CAG / 6.97 / 1.49 / 8.04 / 1.29 / 18.50 / 0.56 / 18.13 / 0.57 / 10.40 / 3.98 / 2.07 / 1.76
CAA / 30.14 / 0.50 / 34.64 / 0.43 / 20.38 / 0.74 / 19.31 / 0.78 / 15.00 / 5.13 / 0 / 0
His / CAT / 16.41 / 1.22 / 15.14 / 1.32 / 15.74 / 1.27 / 16.18 / 1.24 / 20.00 / 23.74 / 22.73 / 15.82
CAC / 4.76 / 2.56 / 3.34 / 3.65 / 7.53 / 1.62 / 8.89 / 1.37 / 12.20 / 7.35 / 8.26 / 5.27
Leu / CTG / 3.39 / 4.31 / 4.16 / 3.51 / 23.00 / 0.63 / 23.66 / 0.62 / 14.60 / 11.33 / 4.13 / 7.03
CTA / 10.67 / 1.74 / 11.83 / 1.57 / 4.87 / 3.82 / 3.40 / 5.47 / 18.60 / 0.77 / 2.07 / 0
CTT / 17.87 / 1.60 / 14.67 / 1.95 / 21.79 / 1.31 / 18.23 / 1.57 / 28.60 / 28.02 / 16.53 / 19.33
CTC / 4.39 / 4.37 / 3.46 / 5.55 / 10.67 / 1.80 / 12.90 / 1.49 / 19.20 / 28.25 / 22.73 / 24.60
Pro / CCG / 7.31 / 0.83 / 6.13 / 1.00 / 16.33 / 0.37 / 19.60 / 0.31 / 6.10 / 0.69 / 0 / 0
CCA / 16.11 / 0.75 / 17.61 / 0.69 / 7.11 / 1.70 / 4.89 / 2.47 / 12.10 / 11.26 / 8.26 / 3.51
CCT / 9.31 / 1.75 / 12.24 / 1.33 / 10.6 / 1.54 / 8.99 / 1.81 / 16.30 / 2.68 / 0 / 0
CCC / 1.22 / 6.39 / 1.64 / 4.76 / 3.50 / 2.23 / 3.91 / 1.99 / 7.80 / 10.41 / 4.13 / 14.06

a, Average number of codons found per 1,000 codons.

b, Average number of codons found in phage / average number of codons found in bacteria per 1,000 codons

c, Number of codons found in late genes (ORF1 - 25 and 210 - 220) in parenthesis.