Supplementary Figure 1

Supplementary Figures

Fig S1

Supplementary Figure 1

Structure of the bipartite nuclear localization signals in human and mouse p53 (A) and Genotyping of p53NLS1 animals (B, C) A. The nuclear localization of the p53 protein is regulated by three nuclear localization (NLS) sequences, which are conserved in human and mouse. Nuclear localization signals are underlined. Lys305 and Arg306 in human p53 (circled) and a spacer region also regulate nuclear import. The Lysines mutated in p53NLS1 animals are boxed. B. Diagram of the mutation generated in exon 9 of p53. Lysines 316, 317 and 318 are mutated to Alanines. The Not1 restriction endonuclease site is underlined. C. The presence of the mutation was determined by PCR amplification of exon 9 and gain of the Not1 restriction site. Wild-type PCR product (500 base pairs) contains no Not1. The wild-type PCR product when digested with Not1 yields one fragment, (477 bp). The mutated PCR product yields two DNA fragments (279 bp and 198 bp) following Not1 digestion.