COURSE OF STUDY
M.Sc. in MOLECULAR & HUMAN GENETICS
(Semester System)
Candidates who have passed B.Sc. or B.Sc. (Hons) or B. Sc. (Ag.) or M.B.B.S. from a recognized University or any other examination considered equivalent will be eligible for admission to M.Sc. course in Molecular & Human Genetics. The M.Sc. course shall be completed in FOUR semesters.
List of Core, Major Elective and Minor Electives and their Credits
CORE COURSES / Theory / Lab. ExerciseMHG C 101 Transmission Genetics / 3 (MHG C 101 T) / 1 (MHG C 101) L
MHG C 102 Basic Human Genetics / 3 (MHG C 102 T) / 1 (MHG C 102 L)
MHG C 103 Cell Biology / 2.5 (MHG C 103 T) / 1 (MHG C 103 L)
MHG C 104 Molecular Genetics I / 2.5 (MHG C 104 T) / 0.5 (MHG C 104L)
MHG C 105 DNA Technology & Genetic Engineering I / 2.5 (MHG C 105 T) / 1 (MHG C 105L)
MHG C 106 Biochemistry & Metabolic Disorders I / 2 (MHG C 106 T) / 1 (MHG C 106 L)
MHG C 107 Instrumentation / 1.5 (MHG C 107 T) / 0.5 (MHG C 107 L)
MHG C 208 Cytogenetics / 3 (MHG C 208 T) / 1 (MHG C 208 L)
MHG C 209 Molecular Genetics II / 2.5 (MHG C 209 T) / 1 (MHG C 209 L)
MHG C 210 DNA Technology & Genetic Engineering II / 2.5 (MHG C 210 T) / 1 (MHG C 210 L)
MHG C 211 Biochemistry & Metabolic Disorders II / 2 (MHG C 211 T) / 1 (MHG C 211 L)
MHG C 212 Model Genetic Systems / 1 (MHG C 212 T) / 0.5 (MHG C 212 L)
MHG C 213 Bioinformatics / 1 (MHG C 213 T) / 1 (MHG C 213 L)
MHG C 314 Human Molecular Genetics / 3 (MHG C 314 T) / 1 (MHG C 314 L)
MHG C 315 Developmental Genetics / 3 (MHG C 315 T) / 1 (MHG C 315 L)
MHG C 316 Behavioural Genetics / 1.5 (MHG C 316 T) / 0.5 (MHG C 316 L)
MHG C 317 Molecular Genetics of Human Pathogens / 1 (MHG C 317 T) / 0.5 (MHG C 317 L)
MHG C 418 Comprehensive Viva-voce / 2 (MHG C 418 L)
TOTAL CREDITS / 37.5 / 16.5
MAJOR ELECTIVES
MHG MA 301 Clinical Genetics / 3 (MHG MA 301 T) / 1 (MHG MA 301 L)
MHG MA 302 Immunogenetics / 2.5 (MHG MA 302 T) / 0.5 (MHG MA 302 L)
MHG MA 303 Population & Evolutionary Genetics / 2.5 (MHG MA 303 T)
MHG MA 304 Genetic Counseling / 1 (MHG MA 304 T) / 0.5 (MHG MA 304 L)
MHG MA 405 Human Genome / 3 (MHG MA 405 T) / 1 (MHG MA 405 L)
MHG MA 406 Cancer Genetics / 1.5 (MHG MA 406 T) / 0.5 (MHG MA 406 L)
MHG MA 407 Clinical Assignments / 4 (MHG MA 407 L)
MHG MA 408 Dissertation / 6 (MHG MA 408 L)
TOTAL CREDITS / 13.5 / 13.5
MINOR ELECTIVES
MHG MI 01 Techniques in Drosophila Genetics / 2
MHG MI 02 Stress Biology / 2
MHG MI 03 Reproductive Genetics / 2
MHG MI 04 Seminar / 1.5
MHG MI 05 Formulation of a Research Proposal / 1.5
TOTAL CREDITS / 9
(Note: The laboratory exercises will be based on respective theory courses)
Distribution of Courses in different Semesters
SEMESTER I (Total Credits 23)
CORE (23 credits) Theory Lab. Exercise
MHG C 101 Transmission Genetics 3 1
MHG C 102 Basic Human Genetics 3 1
MHG C 103 Cell Biology 2.5 1
MHG C 104 Molecular Genetics I 2.5 0.5
MHG C 105 DNA Technology & Genetic Engineering I 2.5 1
MHG C 106 Biochemistry & Metabolic Disorders I 2 1
MHG C 107 Instrumentation 1.5 0.5
TOTAL 17 6 = 23
SEMESTER II (Total credits 21.5)
CORE (17.5 credits)
MHG C 208 Cytogenetics 3 1
MHG C 209 Molecular Genetics II 2.5 1
MHG C 210 DNA Technology & Genetic Engineering II 2.5 1
MHG C 211 Biochemistry & Metabolic Disorders II 2 1
MHG C 212 Model Genetic Systems 1 0.5
MHG C 213 Bioinformatics 1 1
MINOR ELECTIVES (4 credits 4 -
TOTAL 16 5.5 = 21.5
SEMESTER III (Total credits 22.5)
CORE (11.5 credits)
MHG C 314 Human Molecular Genetics 3 1
MHG C 315 Developmental Genetics 3 1
MHG C 316 Behavioural Genetics 1.5 0.5
MHG C 317 Molecular Genetics of Human Pathogens 1 0.5
MAJOR ELECTIVES (11)
MHG MA 301 Clinical Genetics 3 1
MHG MA 302 Immunogenetics 2.5 0.5
MHG MA 303 Population & Evolutionary Genetics 2.5 -
MHG MA 304 Genetic Counseling 1 0.5
TOTAL 17.5 5 = 22.5
SEMESTER IV (Total credits = 23)
CORE (2 credits)
MHG C 418 Comprehensive Viva-voce 2
MAJOR ELECTIVES (16 credits)
MHG MA 405 Human Genome 3 1
MHG MA 406 Cancer Genetics 1.5 0.5
MHG MA 407 Clinical Assignments - 4
MHG MA 408 Dissertation - 6
MINOR ELECTIVES ( 5 credits) 5 -
TOTAL 9.5 13.5 = 23
MINOR ELECTIVES
MHG MI 01 Techniques in Drosophila Genetics 2
MHG MI 02 Stress Biology 2
MHG MI 03 Reproductive Genetics 2
MHG MI 04 Seminar 1.5
MHG MI 05 Formulation of a Research Proposal 1.5
CORE COURSES
COURSE MHG C 101: Transmission Genetics (3 + 1 Credits)
1. Introduction to Genetics 1
2. Mendelism 9
2.1 Mendel and his experiments
2.2 Law of segregation
2.3 Law of independent assortment
2.4 Application of laws of probability (product rule, sum rule)
2.5 Chromosomal basis of segregation and independent assortment
3. Chi-square test and its application in analysis of genetic data 1
4. Extensions of Mendelism 9
4.1 Allelic variation and gene function- Dominance relationships, basis of dominant and recessive mutations
4.2 Multiple allelism, allelic series
4.3 Testing gene mutations for allelism: complementation test, intragenic complementation
4.4 Visible, sterile and lethal mutations
4.5 Genotype to phenotype: effect of the environment on phenotype development- Penetrance and expressivity, phenocopy
4.6 Gene interactions and modifying genes
4.7 Pleiotropy
5. Sex-linked inheritance, Linkage and crossing over 10
5.1 Genetic recombination and construction of genetic maps in Drosophila
5.2 Interference and coincidence
5.3 Cytological demonstration of crossing over in Drosophila
5.4 Mitotic recombination
5.5 Intragenic recombination
6. Inheritance of quantitative traits 6
6.1 Continuous and discontinuous variation
6.2 Polygenic inheritance
6.3 Genetic variance, heritability (narrow sense and broad sense)
7. Cytoplasmic inheritance, maternal effects, inheritance due to parasites and symbionts 3
Recommended Books1. / Atherly et al / The Science of Genetics / Saunders / 1999
2. / Brooker / Genetics – Analysis and Principles / Benjamin/Cumings / 1999
3. / Fairbanks et al / Genetics / Wadsworth / 1999
4. / Gardner et al / Principles of Genetics / John Wiley / 1991
5. / Griffiths et al / Modern genetic Analysis / Freeman / 2002
6. / Griffiths et al / An Introduction to Genetic Analysis / Freeman / 2004
7. / Hartl & Jones / Genetics – Principles and Analysis / Jones & Bartlett / 1998
8. / Snustad et al / Principles of Genetics / Wiley and sons / 1998
9. / Strickberger / Genetics / Mcmillan / 1985
10. / Tamarin / Principles of Genetics / WCB / 1996
COURSE MHG C 102: Basic Human Genetics (3 + 1 Credits)
1. History of Human Genetics 1
2. Pedigrees- gathering family history, pedigree symbols, construction of pedigrees, 1
presentation of molecular genetic data in pedigrees
3. Monogenic traits 12
3.1 Autosomal inheritance-dominant, recessive
3.2 Sex-linked inheritance
3.3 Sex-limited and sex-influenced traits
3.4 Mitochondrial inheritance
3.5 MIM number
3.6 Complications to the basic pedigree patterns- nonpenetrance, variable expressivity, pleiotropy, late onset, dominance problems, anticipation, genetic heterogeneity, genomic imprinting and uniparental disomy, spontaneous mutations, mosaicism and chimerism, male lethality, X-inactivation
3.7 Risk assessment; application of Bayes’ theorem
3.8 Allele frequency in population
3.9 Consanguinity and its effects
4. Complex traits 10
4.1 Approaches to analysis of complex traits- ‘Nature vs nurture’, role of family and shared environment, monozygotic and dizygotic twins and adoption studies
4.2 Polygenic inheritance of continuous (quantitative) traits, normal growth charts, Dysmorphology
4.3 Polygenic inheritance of discontinuous (dichotomous) traits- threshold model, liability and recurrence risk
4.4 Genetic susceptibility in multifactorial disorders (alcoholism, diabetes mellitus, obesity)
4.5 Estimation of genetic components of multifactorial traits: empiric risk, heritability, coefficient of relationship
5. Human cytogenetics 10
5.1 Techniques in human chromosome analysis (including molecular techniques)
5.2 Human karyotype: banding, nomenclature of banding
5.3 Pathology of human chromosomes
5.4 Nomenclature of aberrant karyotypes
5.5 Common syndromes due to numerical chromosome changes
5.6 Common syndromes due to structural alterations (translocations, duplications, deletions, microdeletion, fragile sites)
5.7 Common chromosome abnormalities in cancer
5.8 Genetics of fetal wastage
6. Pharmacogenetics and ecogenetics, teratogenetics 5
Recommended Books1. / Connor & Smith / Essentials of Medical Genetics / Blackwell / 1993
2. / Davies / Human Genetic Disease Analysis / IRL / 1993
3. / Emery & Mueller / Elements of Medical Genetics / ELBS / 1992
4. / Gersen & Keagle / The Principles of Clinical Cytogenetics / Humana / 1999
5. / Jorde et al / Medical Genetics / Elsevier / 1998
6. / Korf / Human Genetics / Blackwell / 1996
7. / Lewis / Human Genetics / WCB / 1999
8. / Mange & Mange / Basic Human Genetics / Sinauer Assoc / 1999
9. / Maroni / Molecular and Genetic Analysis of Human Traits / Blackwell / 2001
10. / Nussbaum et al / Genetics in Medicine / Saunders / 2001
11. / Pasternak / An Introduction to Molecular Human Genetics / Fritzgerald / 2000
12. / Prichard & Korf / Medical Genetics at a glance / Blackwell / 2004
13. / Strachan Read / Human Molecular Genetics / Wiley / 1999
14. / Vogel Motulsky / Human Genetics / Springer / 1997
COURSE MHG C 103: Cell Biology (2.5 + 1 Credits)
1. Plasma Membrane: Fluid mosaic model, structure of membrane, transport across membrane and mechanisms of endocytosis and exocytosis 4
2. Endomembrane system 5
2.1. General organization of protein transport within and outside the cell
2.2. Protein sorting and secretion
2.3. Mechanism of intracellular digestion
3. Cytoskeleton 4
3.1. Microfilaments: Structural organization, cell motility and cell shape
3.2. Microtubule: Structural and functional organization, cilia, flagella, centriole
3.3. Intermediate filaments
4. Mitochondria 3
4.1 Ultrastructure
4.2 Chemiosmotic theory and respiratory chain complexes
5. Structure and function of peroxisome 1
6. Structure and biosynthesis of ribosome 2
7. Cell cycle and its regulation 2
8. Cell-Cell Interaction 5
8.1. Cell adhesion molecules
8.2. Cellular junctions
8.3. Extracellular matrix
9. Signal transduction 5
9.1. Intracellular receptor and cell surface receptors
9.2. Signalling via G-protein linked receptors (PKA, PKC, CaM kinase)
9.3. Enzyme linked receptor signaling (Growth factor receptor signaling; JACK-STAT pathway)
9.4. Network and cross-talk between different signal mechanisms
10. Programmed cell death 1
Recommended Books1. / Alberts et al / Essential Cell Biology / Garland / 1998
2. / Alberts et al / Molecular Biology of the Cell / Garland / 2002
3. / Cooper / The Cell: A molecular Approach / ASM Press / 2000
4. / Gilbert / Developmental Biology / Saunders / 2003
5. / Jeremy et al / Biochemistry / Freeman / 2002
6. / Karp / Cell and Molecular Biology / John Wiley / 2002
7. / Lewin / Genes VIII / Pearson / 2004
8. / Lodish et al / Molecular Cell Biology / Freeman / 2004
9. / Pollard & Earnshaw / Cell Biology / Saunders / 2002
10. / Tobin Morcel / Asking about Cells / Saunders / 1997
11. / Wilson & Hunt / The Cell: A Problems Approach / Garland / 2002
COURSE MHG C 104: Molecular Genetics I (2.5 + 0.5 Credits)
1 Properties and evolution of genetic material, flow of genetic information 2
2 Organization of viral and bacterial genomes 3
3 Eukaryotic genome 5
3.1 C-value paradox
3.2 Repetitive DNA
3.3 General concept of a gene
3.4 Gene families
3.5 Non-coding genes
4 Replication in bacterial and eukaryotic chromosomes 5
4.1 DNA polymerases
4.2 Replicons, origin and termination
4.3 Replisome
4.4 Genes controlling replication
5 Transcription 5
5.1 Prokaryotic RNA polymerase, sigma factors, initiation and termination
5.2 Eukaryotic RNA polymerases and their promoters
5.3 Processing of transcripts
6 Translation 2
6.1 General mechanism
6.2 Role of rRNA in translation
7 Regulation of gene expression 11
7.1. Regulation of transcription initiation
7.1.1. Operon and regulon
7.1.2. Positive and negative regulation
7.1.3. Enhancers and promoters
7.1.4. Transcription factors: types, DNA binding motifs
7.2. Regulation by attenuation and anti-termination
7.3. Post transcriptional regulation
7.3.1. Alternative splicing
7.3.2. Transport and targeting of RNA
7.3.3. Post-transcriptional gene silencing
7.4. Translational control and targeting of proteins
7.5. Mechanism of steroid hormone and stress induced gene expressions
Recommended Books1. / Atherly et al / The Science of Genetics / Saunders / 1999
2. / Benjamin / Genetics: A Conceptual Approach / Freeman / 2003
3. / Berg Singer / Genes and Genome / 1998
4. / Black / Microbiology: Principles and Explorations / Wiley / 2002
5. / Dale & Schartz / From genes to Genome / Wiley & Sons / 2003
6. / Griffiths et al / Modern Genetic Analysis / Freeman / 2002
7. / Griffiths et al / An Introduction to Genetic Analysis / Freeman / 2004
8. / Hartl et al / Essential Genetics / Wiley & Sons / 2002
9. / Klug & Cummings / Essentials of Genetics / Prentice Hall / 2003
10. / Latchman / Gene Regulation / Chapman & Hall / 1995
11. / Lewin / Genes VIII / Pearson / 2004
12. / Maloy Freifelder / Microbial Genetics / Jones and Barlett / 1994
13. / Meesfeld / Applied Molecular Genetics / Wiley-Liss / 1999
14. / Ptashne / Genetic Switch / Blackwell / 1986
15. / Russell / Genetics / Benjamin / 2002
16. / Streips & Yasbin / Modern Microbial Genetics / Wiley / 2002
17. / Trun & Trempy / Fundamentals of Bacterial Genetics / Blackwell / 2004
18. / Watson et al / Molecular Biology of the Gene / Pearson / 2004
COURSE MHG C 105: DNA Technology & Genetic Engineering I (2.5 + 1 Credits)
1. Enzymes used in DNA technology 4