Name ______Hr ____

Enzyme Graphing Activity

Amylase is an enzyme that converts starch, by hydrolysis, into sugars. An experiment was performed to determine how rapidly amylase works at different temperatures. The data from this experiment is listed below

Temperature (OC) / Rate of Starch conversion (grams per minute)
0 / 0.0
10 / 0.4
20 / 0.6
30 / 0.8
40 / 1.0
50 / 0.4
60 / 0.2
70 / 0.0

1.  Write a hypothesis that matches the experiment above.

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2.  What dependent variable in this experiment? ______

3.  What is the independent variable in this experiment? ______

4.  Graph the data below.

a.  Temperature should be on the horizontal or X axis.

b.  Rate of conversion should be on the vertical or Y axis.

c.  Don’t forget a title.

5.  What conclusion can be formed about the effect of temperature on the rate of activity of amylase?

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6.  According to the data/graph, at what temperature (optimum) does amylase function most efficiently (best)? ______

7.  Amylase is present in saliva. If you were to chew a cracker for a long time without swallowing it, the cracker would start to taste sweet.

  1. What macromolecules make up the cracker? ______
  1. Explain why the cracker starts to taste sweet in terms of the action of amylase.

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