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EH&S Laboratory Safety Checklist
Building______Room______
Date______Inspector ______
Legendv = Item is in compliance with guidelines
N/A= Not applicable
N/E= Not Evaluated
No = Item may not be in compliance with guidelines
General/Special Items
1. Laboratory has implemented Laboratory Signage Program/Current update
2. Signage used for Biosafety facilities
___ 3. Housekeeping: spills cleaned up, no floor storage, adequate work space
__ 4. No tripping or slipping potentials (NR)
___ 5. Lab is free of food, drink and smoking materials
_ 6. Custodial services performed safely in laboratory (NR)
____ 7. Fire extinguishers present, properly located, and in working order
____ 8. Safety showers are available and within 10 seconds and 50ft of the chemical
hazard
____ 9. Eyewash stations function properly, are positioned adequately, and labeled with
non-potable water signs.
____ 10. First Aid Kits present and stocked properly
_ 11. Laboratory Spill kit properly stocked, when present (NR)
__ 12. Employees are wearing appropriate Personal Protective Equipment
_ 13. Respiratory Protection Program when required
_____ 14. Lab personnel have been properly trained
_____15. Employees subject to Bloodborne Pathogens have attended training
_ 16. Inspections performed on regular basis by supervisor or PI
____ 17. MSDSs are available, and personnel know where they are kept
______18. Laboratory Safety Manual available, information pages (yellow) completed,
and personnel know where it is kept
_____ 19. Laboratory floor plan showing safety equipment available
_____ 20. Training records available for PPE; hazard assessment documents present
_____ 21. List of Permissible Exposure Limits available
___ 22. Chemical Inventory has been updated in the last year/ Backup of inventory can be found in the event of an emergency (NR)
____23.Laboratory sink water faucets are labeled “Caution: Non-Potable Water. DO
NOT DRINK”.
____24. Proper and separate containers provided for glass and sharps
___ 25. Labs with pressurized vessels: Each has been tested/labeled (NR)
_____ 26. Flexible neoprene tubing used for gas burners
_____ 27. Traps are filled weekly to prevent frequent odor problems (NR)
_____ 28. Refrigerator storage is labeled for contents (food,chemical/sample,flammable)
__ 29. Explosion-proof refrigerator used/located properly
___ 30. Ground Fault Circuit Interrupters (GFCI’s) provided, where applicable
____ 31. Environmental monitoring/medical surveillance performed when required
Local Ventilation (Hoods, BSC's, ETC.)
______32. Hoods are labeled with indicator of face velocity within the last year
_ 33. Hood sash kept down when hood not in use (NR)
____ 34. Hoods are clear of excessive storage
_ 35. Hood properly used by operator (NR)
____ 36. Hood valves are working properly
_ 37. Local exhaust provided where needed; canopy hoods for autoclaves/atomic absorption units, etc.
_____ 38. Flammable liquid storage cabinets properly vented (NR)
___ 39. Biosafety cabinet is certified and labeled within one year (NR)
__ 40. Biosafety cabinet is clear of excessive storage (NR)
__ 41. Biosafety cabinets properly used by operator (NR)
__ 42. Only microburners BCSs (NR)
Gases
_____ 43. Cylinders are not stored by sole entrance to lab
_____ 44. All cylinders are standing upright
_____ 45. Cylinders are properly secured to bench or wall
_____ 46. Contents of cylinders are clearly labeled
_____ 47. Unused cylinders are capped
_____ 48. Only the cylinders needed are located in lab
___ 49. Empty cylinders are separated from full cylinders
___ 50. Unused lecture bottles are returned to the manufacturer within one year
_____ 51. Flexible tubing/hoses are in good condition
_____ 52. Tubing is tightly connected to equipment
Hazardous Waste
_____ 53. "Hazardous Waste" containers are properly labeled
_____ 54. Waste is completely and accurately described
_____ 55. Waste is compatible with the container
___ 56. Secondary containment is provided for waste (NR)
_____ 57. Bottles are filled to a safe level
_____ 58. Waste containers closed when not in use
___ 59. Containers are clean on the outside
_____ 60. Containers do not leak
_____ 61. Incompatible wastes are separated from each other
_____ 62. Container labels are filled out as waste is generated
__ 63. Biohazardous waste properly stored
Chemicals
_____ 64. Chemicals are stored in proper containers
_____ 65. Chemical containers are properly labeled
__ 66. Oxidizers are stored away from flammables, reducers
__ 67. Oxidizing materials are stored in a non-combustible cabinet
__ 68. Peroxidizable compounds expiration dates are clearly marked on
containers(NR)
__ 69. Peroxidizable compounds checked for peroxides/discarded after pre-
determined maximum period of storage(NR)
_____ 70. Hydrofluoric Acid and Perchloric Acid not used in standard fumehood (NR)
_____ 71. Flammable liquids are properly stored
_____ 72. Volume limits for flammable solvents not exceeded
___ 73. Unusable or excess stock is turned in as waste or for reissue (NR)
___ 74. Standard operating procedures developed for individual experiments working
with particularly hazardous chemicals, carcinogens, reproductive toxins, etc.