Biology EOC Review
NAME______
Goal 1: Learner will develop abilities necessary to do and understand scientific inquiry.1.01 Identify biological problems and questions that can be answered through scientific investigations.
1.02 Design and conduct scientific investigations to answer biological questions (create testable hypotheses, identify variables, use a control or comparison group when appropriate, select and use appropriate measurement tools, collect and record data, organize data into charts and graphs, analyze and interpret data, communicate findings).
You have measured the rate at which a fish breaths at various temperatures by counting the rate at which its gills open. The data is below. Graph this data. “Y” axis – breathing rate; “X” axis – Temp.
Breathing rate Temperature
19/min 5 deg C
25/min 10 deg C
30/min 20 deg C
34/min 30 deg C
37/min 35 deg C
What is the independent variable? The dependent variable?
Temperature Breathing rate
What is the best type of graph for this data? Why?
Line (each point has two numbers)
What happens to breathing rate with increase in Temp?
Breathing rate increases
What would be a good control for this experiment?
Measure breathing rate of fish in regular environment
How do you think the breathing rate was measured?
Counting movements of gill cover or mouth openings
What do you think would happen if you raised the
temperature even more?
Fish might die at some point – living systems cannot handle too much increase in T.
Why would it be a bad idea to do this?
Death of fish
1.03 Formulate and revise scientific explanations and models of biological phenomena using logic and evidence to: explain observations, make inferences and predictions, explain the relationship between evidence and explanation.
An experiment was done that measured the Effects of Nitrates on the Growth of Algae. Growth of algae was determined by how well the water transmitted light. The less the light transmission, the greater the algae growth blocking the transmission of light. Important!
Looking at the graph on the next page what conclusions can you draw about algae growth and nitrates in the water? As weeks progress, the level of nitrate has a bigger impact on algae growth.
What other data would you like to have? Optimum nitrate for algae growth, temperature, amount of light
What would you predict would happen if this same data was gathered at the end of week 8?
The algae might have overgrown and died – light transmission might increase.
Why do you think that nitrates have this effect on algae growth?
Nitrates are fertilizers; improve growth; needed for building amino acids and nucleic acids
1.04 Apply safety procedures in the laboratory and in field studies. (Recognize and avoid potential hazards, safely manipulate materials and equipment needed for scientific investigations.)
What kind of care must be taken when working with bacteria?
Use gloves; goggles; do not expose bacteria to air unnecessarily.
Why must care be used when working with bacteria?
Bacteria can cause disease and should be handled with care.
What are the issues surrounding the use of animals for research?
Answers will vary – animals should not be tortured or used in a way that causes great pain.
1.05 Analyze reports of scientific investigations from an informed scientifically literate viewpoint including considerations of: appropriate sample, adequacy of experimental controls, replication of findings, and alternative interpretations of the data.
Read the following article and answer the questions.
SPINACH MAY CUT STOMACH ULCER RISK - May 15, 2008
Vegetables rich in nitrates, such as spinach, may help to protect against stomach ulcers thanks to bacteria in the mouth, a Swedish study suggests. The work challenges earlier suggestions that a diet rich in nitrates could pose a health risk.
Joel Petersson was awarded his PhD by theUniversity ofUppsala on May 9 for the study, which shows that rats fed on a nitrate-rich diet had a thicker layer of mucus lining their stomachs, protecting them from hydrochloric acid in gastric juice and cutting the risk of ulcers.
Petersson found that mouth bacteria play a vital part in the process. Nitrates in food are absorbed in the gut and enter the blood stream. From here they get into saliva but are reduced to nitrites by oral bacteria. After being swallowed, the nitrites are reduced to nitric oxide by stomach acid. Nitric oxide, an important signaling molecule, triggers an increase in the flow of blood to the stomach, helping to renew and thicken its mucus lining.
When Petersson gave rats an antibacterial mouthwash to kill the oral bacteria, he found they were more vulnerable to stomach ulcers.He suggests that people using these mouthwashes regularly may be at risk, especially if they are also frequent users of nonsteroidal pain killers like aspirin which can also damage the stomach lining. 'There are other much safer ways of blocking the production of the sulphur-containing compounds in the mouth if you have bad breath,' he said.
Between 60 and 80 per cent of the nitrates consumed in a normal Western diet come from vegetables, with beetroot, celery and spinach containing particularly high-levels of 1-3g per kilo.
Studies in the 1970s suggested a link between high nitrate levels in drinking water and both stomach cancer and the rare blood condition in babies, methaemoglobinaemia. 'We have since wasted millions in trying to reduce nitrate levels in drinking water when there is no real evidence to show that it is harmful to humans. If you do eat a lot of nitrate it is very easily dealt with - you just pee it out,' Petersson said.
"The Swedish study has shown this further effect in animals and I would certainly expect the same mechanism to exist in the human stomach "
- Nigel Benjamin
Nigel 'Ben' Benjamin, now a consultant in acute medicine at thePeninsulaMedicalSchool inPlymouth,UK, discovered a different protective mechanism for nitrates in the 1990s. He showed that the combination of nitric oxide and acid controlled the growth of dangerous bacteria like salmonella in the gut. 'The Swedish study has shown this further effect in animals and I would certainly expect the same mechanism to exist in the human stomach,' said Benjamin. 'This is exciting work and gives us further reasons for eating a diet that contains lots of fresh vegetables.'
John Bonner
QUESTIONS:
The experiment above was done on rats. Is there any reason to think that it might apply to humans.
Rats are mammals like humans and their genetics and functions are similar.
What else would you like to know about this topic before you start eating spinach every day?
Have other experiments confirmed these results; are there any harmful effects to eating a lot of spinach?
There is conflicting data from the 1970’s. How do you decide what is the most scientifically accurate?
Look for further studies. Look for recent studies.
Goal 2: Learner will develop an understanding of the physical, chemical and cellular basis of life.2.01 Compare and contrast the structure and functions of the following organic molecules:
Macromolecules / Function / SubunitsCarbohydrates / Quick energy; plant cell walls / Monosaccharides such as glucose
Proteins / Serve as enzymes, antibodies, plasma membrane inclusions; carrier molecules (hemoglobin); structural / Amino acids
Lipids / Long term energy storage, insulation, plasma membranes / Glycerol and 3 fatty acids
Nucleic Acids / Carry instructions for making proteins; also carry those instructions to the ribosomes / Nucleotides (sugar, phosphate, Nit. base)
Specific Molecule / Function / Subunits
Starch
carbohydrate / Plants store glucose as starch – source of energy / glucose
Cellulose
carbohydrate / Form the cell walls of plants / glucose
Insulin
protein / Used to help store glucose in liver as glycogen / Amino acids
Glycogen
carbohydrate / Animals store glucose as glycogen – source of energy / Glucose
Glucose
carbohydrate / Simple sugar used in cellular respiration (aerobic and anaerobic); energy is released
Enzymes
protein / Catalysts for chemical reactions (both synthesis and decomposition) / Amino acids
Hemoglobin
protein / Oxygen carrier molecule found in the blood / Amino acids
Fats
lipids / Used for long term energy storage and insulation – as well as forming cell membranes / Glycogen and fatty acids
DNA
Nucleic acid / Found in nucleus – carries instructions for making proteins / nucleotides
RNA
Nucleic acid / Form the ribosomes, forms mRNA to carry genetic info to ribosome, forms tRNA which carry amino acids to ribo. / nucleotides
Describe the following nutrient tests:
Nutrient / Type of Test / Negative Test / Positive TestStarch / For presence of starch / Stays brownish gold / Turns blue black
Lipids / Brown paper bag / No greasy mark / Has a greasy mark
Monosaccharides / Benedict’s Test / Stays turquoise blue / Turns brick red (orange)
Protein / Biuret’s test / Stays deep purplish blue / Turns pinkish violet
Explain the importance of shape to enzyme function. Enzymes function by lock and key – each enzyme has to fit the molecules that it joins together or breaks apart; if it does not fit, it cannot function.
Explain what determines the shape of an enzyme.
The order of the amino acids determines the secondary and tertiary levels of structure.
Explain why enzymes are specific.
2.02 Investigate and describe the structure and function of cells including cell organelles, cell specialization, and communication among cells within an organism.
Fill in this chart. Also give the letter or number of the part as seen in the diagrams below.
Cell Part and Letter / Structure Description / FunctionNucleus
A, 6 / Porous nuclear envelope; contains chromosomes (DNA) and nucleolus / Carries code for what proteins to make (inherited information)
Plasma Membrane
K, near 11 / Phospholipid bilayer with embedded proteins, etc. / Regulates what leaves and enters cell
Cell wall
PLANTS only J / Composed of cellulose; surrounds plasma membrane / Gives structure to plant cells
Mitochondria
L, 1 / Two membrane layers; inner membrane folded to incr. surface area / Where parts of aerobic cellular respiration takes place
Vacuoles G, 3
Large in PLANTS / Membrane bound sacs / Sacs -hold food or water; in animals, small vesicles hold materials
Chloroplasts I
PLANTS only / Structures containing stroma and grana / Site of photosynthesis
Ribosomes
F, 13 / Small beads of RNA found on ER or in cytoplasm / Site of protein synthesis (“read” the code found on mRNA)
Which cell is the plant cell (left or right)? (LEFT)
Which structures are found only in the plant cell? Chloroplast, cell wall, large central water vacuole
Which structures are found only in the animal cell? centriole
Microscope Use:
Put the following steps for making a wet mount slide in order.
A. Once the object is located, without moving the adjustment, change to medium power
B. Put the tissue on the slide
C. Switch to high power and bring the object into clear focus again.
D. Add a coverslip
E. Place the slide on the stage of the microscope
F. Add a drop of water
G. Try to locate the object using low power and coarse adjustment
H. Use fine adjustment to bring the object into clear focus.
1) ___B__ 2) __F___ 3) __D ___ 4) _ E ____ 5) __G___ 6) __A___ 7) __H___ 8) _C____
How do you determine total magnification of a microscope? (Assume the eyepiece magnifies 10 x and the objective magnifies 40 x) 10 times 40 = 400x
Draw how the letter “e” would look as view through a microscope? Upside down and reversed left to right
Put the following in order from smallest to largest:
Organ systems Cells Organs Tissues
__cells______tissues___ _organs______organ systems__
Below are a variety of cells from the human body.
Label these cells (red blood cell, sperm cell, white blood cell, muscle cell, nerve cell)
Nerve cell on left, sperm is top middle, white blood cell is lower middle, red blood cell top right, muscle cell bottom right
Which cell is adapted for movement? What structure makes this movement possible? What organelle is very plentiful in these cells in order to provide the energy for movement?
Sperm cell; flagellum; mitochondrion (for cellular respiration)
Which cell has no nucleus? What is the function of this cell?
Red blood cell; to carry oxygen and remove carbon dioxide from cells
Which cell is involved in the immune system?
White blood cell (B cells, T cells, macrophages)
Which cell helps in movement of bones? What happens in these cells to make that movement possible?
Muscle cell; contraction involving muscle proteins (myosin and actin)
Which cell is adapted for transmitting messages? What is the direction of these messages? How do the messages get from one cell to the next? Neuron (nerve cell); from top (dendrites) to bottom (axon); neurotransmitters
Hormones:
What structures produce hormones?
Glands
How do hormones travel throughout a body?
Through the circulatory system
What is the function of hormones?
Regulation of body functions; growth; metabolism, etc.
What is a feedback mechanism? When hormone product increases that concentration slows production of the hormone; if hormone product decreases, that concentration triggers release of hormone (negative feedback)
The diagram below shows many proteins and other molecules embedded in a cell membrane.
What are some of the functions of these proteins and other molecules? Molecule channels; proton pumps; some enzymes/hormones (temporary); antigens for cell recognition; connection to other cells;
2.03 Investigate and analyze the cell as a living system including: maintenance of homeostasis, movement of materials into and out of cells, and energy use and release in biochemical reactions.
Explain what has happened in the diagram to the left. Water passed to right; starch could not move through membrane – too large.
Why did the large dark molecules NOT move to the left? Too large for membrane pores
How is the semipermeable membrane like a cell membrane? Cell membranes have pores that selectively move materials in and out.
If the dark molecule is starch, where is the starch concentration greatest (left or right)?
If the white molecule is water, where is the water concentration greatest at first? On left