DNA template

Sequencing primer

Ready Reaction Mix

2.5xsequencing buffer,ieHazardous Substances Policy - Assessment

CHEMICAL HAZARD AND RISK ASSESSMENT

School of Biosciences

Name of supervisor / Steve Busby / Assessment Number* / SJWBJM0014
2107
Signature
Assessor / Jennie Mitchell / Date of Assessment / 08/12/02

Signature

Notes A School COSHH form in Word is available on the School Server.

Available from the Health and Safety Unit.

Guidance on making an assessment is given in Making a Chemical Hazard and Risk Assessment.

Guidance is also available from Guidance on Completing the Chemical Hazard and Risk Assessment Form.

Use a continuation sheet to expand any section of this form in hard copy version.

1 / LOCATION OF THE WORK ACTIVITY / G3/T101/Genomics Lab
2 / PERSONS WHO MAY BE AT RISK
List names where possible / All members of the group (see current members list at front of folder) and
the Genomics lab.
3 / ACTIVITY ASSESSED / Big Dye Terminator sequencing
4 / MATERIALS INVOLVED / Attach copies of data sheet(s)
NAME / AMOUNT per sample* / HAZARD / RISK PHRASES / HAZDAT NO*** / BIOSCIENCESNO***
DNA template / 200-500ng / See GMMO assessment.
Sequencing primer / 3.2pmol / See GMMO assessment
Ready Reaction Mix / 4ul / N/A
2.5xsequencing buffer,ie / 4ul / N/A
200mM Tris-HCl [pH 9.0], 5mM MgCl2
Sterile Water / up to 20ul / N/A
75% Isopropanol / 80ul / FLAMMABLE / R11- Highly Flammable / 127
IRRITANT / R36-Irritating to the eyes
R67-Vapours may cause drowsiness and dizziness
70% Ethanol / 250ul / FLAMMABLE / R11- Highly Flammable / 84
Hi-Di Formamide
99.5% formamide
0.11% EDTA
0.39% water / 10ul / TOXIC
IRRITANT / R61-May cause harm to the unborn child. Category
2 risk to reproduction
R36/37/38-Irritating to eyes, respiratory system and skin. / 261
5 / INTENDED USE
Give brief details and attach protocol/instructions / Sequencing plasmid DNA samples using the Big Dye Terminator
kit and the 3700 DNA Analyser in the Genomics lab.
6 / RISKS to HEALTH and SAFETY from INTENDED USE
From personal exposure or hazardous reactions. Refer to OELs, flash points, etc., as appropriate. Are pregnant women, breast-feeding mothers especially at risk?
There is a fire risk from using 75% Isopropanol (flashpoint 12°C) and 70% ethanol, and a possible risk of irritation to the
eyes from the isopropanol. Large quantities of isopropanol used on the open bench may cause drowsiness
or dizziness. The Hi-Di formamide solution poses a risk to pregnant women especially, and there is also a
risk of irritation to eyes, skin and the respiratory system.
7 / CONCLUSIONS ABOUT RISKS
Is level of risk acceptable? Can risk be prevented or reduced by change of substance/procedure? Are control measures necessary?
The risk of fire needs to be controlled. The risk from the Hi-Di Formamide is very slight due to the very small
volumes used, although this risk is increased when aliquoting from the main stock into tubes.
8 / CONTROL MEASURES
Additional to Good Chemical Practice
All sources of ignition should be removed when working with isopropanol or ethanol. Large volumes of
isopropanol should not be left out on the open bench next to the worker for more than a few minutes.
Tops should be replaced on bottles and tubes as soon as possible. Open bottles and tubes containing
Hi-Di Formamide should not be used in large quantities in the viscinity of pregnant women. Pregnant women themselves who have to use this ought to handle it in a fume hood.
The fume hood should be checked before use to make sure that it is working properly.
When working with solvents stocks, the fume hood should be used.
9 / INSTRUCTION/TRAINING
Specify course(s) and/or special arrangements.
10 / MONITORING
Performance of control measures,
Personal exposure / Health Surveillance
11 / WASTE DISPOSAL PROCEDURE
See School Server for Approved Procedure Document on specific Chemical Waste Disposal.
All waste isopropanol and ethanol should be collected in a waste non-halogenated solvent bottle which
when full should be removed from the lab for specialist disposal. All of the sequencing sample is
loaded onto the 3700 DNA Analyser and so is not returned to the user for disposal. Should there be any
Hi-Di formamide to dispose of, it should also be placed in the Waste Non-Halogenated Solvent bottle.
12 / REVIEW
Enter the date or circumstances for review of assessment (maximum review interval 5 years)
08/12/07
13 / EMERGENCY ACTION
TO CONTROL HAZARDS / To stabilize situation eg spread absorbant on liquid spill; eliminate sources of ignition, etc.
Isopropanol / Ethanol - Shut off all sources of ignition. Soak up small spills with the lab spill kit. Prevent material from entering drains, and guard against static electricity. Transfer solvent to a suitable container.
Hi-Di Formamide – soak up with lab spill kit
All materials from any such spills should be treated as special waste for disposal.
In the event of fire, use carbon dioxide extinguisher.
TO PROTECT PERSONNEL / Evacuation, protection for personnel involved in clean-up, Special First Aid
Evacuate area. The affected area should be ventilated.
Protective clothing, rubber boots and gloves, and self-contained breathing apparatus should be worn.
Ethanol or isopropanol – avoid build up of static electricity.
In the event of contact with eyes or skin, the affected areas should be washed with copious amounts of water.
TO RENDER SITE OF EMERGENCY SAFE / Clean-up/decontamination
Thoroughly wash the contaminated area with lots of water in all cases.
CONTACT PHO

* Prefix T is used for Teaching Assessment Number.

** List the amount by weight of the substance used. (for liquids eg; 100 mls 1M Sodium Hydroxide =

4g).

*** Hazdat No is the UNICOSHH datasheet report number.

Biosciences No is the Biosciences data sheet number.

UNICOSHH IS A CHEMICAL DATABASE ON THE HEALTH AND SAFETY UNIT SERVER.

BIOSCIENCES DATA SHEETS ARE AVAILABLE IN THE SCHOOL SAFETY OFFICE.