Protein Guided Notes
Macromolecule:
Atoms Found in the Molecule:
Functions & Examples:
Function / ExampleMonomer/Building:
-Draw & label an amino acid below
-What type of bond is formed when amino acids are linked together?
Important Info:
n ______determines the ______of amino acids in a protein or polypeptide chain.
n Sequence of amino acids is ______to each protein
Protein Testing
Procedure:
1. Label 4 test tubes 1, 2, 3, and 4.
2. Add 15 drops of gelatin to test tube 1.
3. Add 15 drops of glucose to test tube 2.
4. Add 15 drops of water to test tube 3.
5. Add 15 drops of egg whites to test tube 4.
6. Record the color of each test tube in your data table.
7. Add 5 drops of Biuret reagent to each test tube.
CAUTION: Biuret reagent is extremely caustic to the skin and clothing. If spilled, rinse with water and call your teacher. 8. Record the color of each test tube in the data table. 9. In the last column of your data table, write a plus (+) if there is a protein present, and a minus (-) if there is not a protein present.** When Biuret reagent is mixed with a protein, it will produce a lavender to violet color. 10. Discard contents of the test tube in the sink with a lot of water. Wash the test tube with test tube brush.
Test for Proteins
Test tube / Substance / Color at start / Color after adding Biuret reagent / Protein present (+/-)1 / Gelatin
2 / Glucose
3 / Water
4 / Egg Whites
Conclusion:
1. Which solutions contained protein?
2. Biuret reagent will turn the skin brownish-purple. Why does this happen?
3. What was the control group?
4. What was the independent variable?
5. What was the dependent variable?
Lipid Testing
Purpose: To test various foods for the presence of lipids.
Procedure:
1. Divide a piece of a brown paper lunch bag into six equal sections. See example provided by your teacher.
2. In each section of the paper, rub a small amount of each of the following foods... If it is a liquid, just add a couple of drops to make a wet spot.
a. apple juice
b. butter
c. egg
d. honey
e. corn oil
f. leave one section empty
3. Take a clean paper towel and rub off any excess food left on the paper. DON’T MIX THE SAMPLES.
4. Label each section of the paper so you know which foods were placed in each section.
5. Let the paper dry for about 15 minutes.
6. Hold the paper up to the light and check to see which foods contained the lipids. If there is a translucent spot on the paper where the food was placed, the food contains lipids.
Conclusion:
1. Which of the foods contained lipids?
2. What types of lipids were observed today? Use your notes on lipids to help you classify the type of lipid.
3. What was the independent variable?
4. What was the dependent variable?
5. What was the control group?
Lipids Name ______
1. Lipids are not polymers, but they are organic macromolecules. The main types vary in structure. Which 3 elements do all lipids have in common?
2. What are three main types of lipids and what are their main functions?
List the three types of lipids. What are the main functions of each type of lipid?
1. a.
2. b.
3. c.
3. What causes fatty acids to be saturated and unsaturated?
______
4. What does it mean that fatty acid chains are hydrophobic?
______
5. For the three pictures below, write the kind of lipid shown. For fats, specify saturated and unsaturated fats.
a. b. c. ______
6. Lipids are used in living organisms . Circle the appropriate answer(s).
a. as parts of biological membranes.
b. to store energy.
c. to regulate chemical reactions.
d. as chemical messengers.
7. Circle the parts of the molecule that need to combine through dehydration synthesis (polymerization) to form a saturated fat. Label which are fatty acids and which is glycerol.
Nucleic Acid Guided Notes
Macromolecule:
Atoms Found in the Molecule:
Subunit/Building Block/Monomer:
-Draw and label the nucleotide with the following words: nitrogen base, sugar, phosphate group
Function:
Examples:
Food Label Evaluation
Milk
1. Which organic molecules are found in milk? (lipids, carbs, proteins)?
2. Is there cellulose in milk?
3. What type of carbohydrates are found in milk? (simple sugars or complex carbs?)
4. How does milk do a body good? Use the nutrition info and give SPECIFIC examples of HOW.
Butter:
1. Which organic molecules are found in butter?
2. What kinds of lipids (specifically) are found in butter?
3. How can butter/ lipids help your body?
Honey:
1. Which organic molecules are found in honey?
2. What types of carbohydrates are found in honey?
McDonald’s Big Mac
1. What types of organic molecules are found in a Big Mac?
2. What types of lipids are found in a Big Mac?
3. What type of molecule is cholesterol? (go back to your concept map!)
4. What type of carbohydrates does a Big Mac have?
Ruffles Baked Potato Chips
1. Are there more carbohydrates or more fat in baked chips?
2. What kind of carbohydrates do we find in baked chips? (mono/ simple sugars, poly/ starch/ cellulose)