96-well PCR Purification_revised

96-Well PCR Purification & Sequencing: Sample Submission

Please note:

This service can also be ordered for smaller sample sets (as long as submitted in plate-format!). In this case, the full plate price will have to be charged as our automated procedure requires the same amount of reagents and instrument time for both partial and full 96-well plates.

Sample Preparation:

  • Unpurified PCR reactions MUST be submitted in a sturdy, half-skirted 0.2 ml 96-well plate.

Plates can be purchased from USA Scientific (TempPlate semi-skirted 96-well PCR plate; Item No.: 1402-9700).

(Link:

  • Each well of the plateMUST contain 10 ulof sample.
  • Please arrange your samples horizontally (in rows) in the PCR plate. Proceed from well A1 to A12, from B1 to B12, from C1 to C12, etc.
  • Please do not leave any empty wells between samples, and please do not leave any empty spaces between samples on the order form.
  • The order form must match the order and position of samples in the PCR plate.
  • Please label the plate as clearly as possible - with a maximum of 5 alpha-numeric characters (for example: 5B001).
  • Tightly seal your plate(s) with an adhesive tape/foil to prevent samples from drying and/or cross-contamination.
  • For each 96-well plate containing unpurified PCR reactions, please submit a separate tube containing 400 ul of the appropriate sequencing primer(xxx).

Please note:

If you are planning to submit multiple sequencing primerswith your plate of unpurified PCR reactions, please arrange your primer set in a separate 96-well PCR plate (at least 10 ul of axx primer solution). Well positions of primers must match the well positions of your PCR reactions.

  • Place your 96-Well PCR Purification & Sequencing order online and include a printed copy of the barcoded order form with your sample submission.

Sample Shipment:

Please use one of our drop boxes (at various MGH locations and at our facility).

If direct sample drop-off is not an option for you, please ship your samples at room temperature, with the completed order form, via FEDEX overnight service to the CCIB DNA Core facility. We strongly recommend that you wrap or cushion sample tubes and plates with Kimwipe tissue or bubble foil to avoid breakage during shipping.

Shipping Address: CCIB DNA Core Facility

38 Sidney Street, Suite 100

Cambridge, MA 02139

IMPORTANT:Please note that we are not receiving samples on Saturdays and Sundays.