We transformed the two candidate genes SbWRKY and SbTATA from genetically-dominant S. propinquum BAC YRL20H16 into recessive non-shattering sorghum cultivar Tx430, each construct including coding sequences and about 1 kb of 5`- and 3`- flanking sequences. Sequences from SpWRKY and SpTATA are attached below, respectively.
>SpWRKY-construct
1 TAAGATGACT CTATTTTTTA TCAATAAGCA CTTTGTACTA TGATTAAGAC AAAAGGAAGA
61 GAGGGGACAA GAATTACAAA CTATACTTAG GGGTTGTTTG AATTTCAGTC ATAGTTGGTC
121 ACAACTCAGA TGTGGTGAGA CACACTCTAT GATGAGAATA ATGAGATCTG TTTGGTTCTC
181 TTCTCACCTA GGCTACATCG CATCTGGAGC GAGAGACAGG CTAGCCACAG CCTGGTCTGG
241 TGCATGCACC TGCACTTGTT TGGTTTTGCT CTTTGTTTTG AGCCACTCCA GCCATGTCTC
301 GGAAAGATAT TGTTTTGTTG GTCTTTGGCT TGGCACCAGT GCTCTCTCAC GCGTACAGGC
361 ACACGCTCTC TTTTGGCTCC ACGCAGCCAT GTGTTGGCTA AAAATGATTT TAGAATCCAT
421 TTCCCATGAG CCTGAGATGG TTGCACGCAC TATAGGTCTA ACCCTGGTAG CACTTTAGGT
481 AACCAAACAC CTTAAGCCTG CATCCCAAGA GCCAGGCCAG TTTGGAAACT GGACAACCAA
541 ATAGGCCTCT AATGAATTTG ATGTGTTGTA TTCTGTGGGT GTCTAGCACT CTTCACCAAC
601 TAAACACTGA TAAAAAAAAG TTATGGTGTG CGATGCCTTA GTGTGGCATA GCAAGTGAAG
661 GCCGGGAACC AAACATGCTT TTACTCTTTC ATATCTTAGG CCATGTTTGG TTTGTCGTAG
721 TAAACTTTAA CTTCCATCAC ATCAAATATT TGAGCACATG CATAGAGTAC TAAATATATA
781 GACTATTTAC AAAATTAAAA ACACAACTAG AGAATAATTT ATGAGACAAG TTTTCTGAGC
841 CTAATTAGTC TATGATTGGA CACTAATTGT CAAATAAAAT AAAAATACTA TAATACCTAT
901 TAAACTTTAA TACCTTCGAC CAAACAAGCC CTTACAGGGT TTCAAATATG TATATAAAAT
961 TATTTTCGTT AAGCTTTCAT ATTAAACTTC TCATTGTTGT CTCATTACCA TCTTTCCCTG
1021 CAAAATGTGA AAACAAGGTG GATAAATACA TGAATCCACA TCTGTTCTCA CCCCTAGTAT
1081 TTAGTAAAAG GAAATAGTGT ACTCTCTCAA GTACAAATAA TAATGTTTCT TGACTTCAAC
1141 ACCTCTAACA CAAAATCGTA ACTAATATTA TTTGTGTAAT AATATATATC TATAAAAGAA
1201 CATGTTGCCT CTCTCTAGAA AAGTCTACCT CTTGATGTCA TTTTCCAAAT ATCAAAACTC
1261 GATACACAAA AGAATTGATT TAGAACCAAA GATTAAAATG CCTGACTACA TGATGAAACC
1321 TGAAAACATT GTTCTATTAT TAGTGACTGA AGGGAGTAAT ATCCAACAGT AACTTCTTGT
1381 TGCGAAGATT AGTGTTGTAC GCAAAAAGAA ATATCCATAT TCCTCCATAT AAAGGAGATG
1441 ATGAGATCAC AGTGATTTTC TGGTTCAGTC AAAACCAGTG GCAAAGTTGG GTAGGGAATT
1501 GAAGCATGTG AACCCAAAAA TTTACTGATT CGTCTTCGTC TTGACGACGT TAACGTCGTC
1561 GCATCTGAGA AACTTCCATT CGATTGACTA ATAAGCCCTG ATAATAAATA TACCACACCC
1621 AAAGAGCTTC ATCACTACTC TCTCAATCTC TCTCCCTCTC GTCTACATGG TTCATTCATT
1681 AAACTTTGCG ACAACATGGG AGCAGCAGTA GAGCACAGGA CGTCGTAGAC GTACGGTCAC
1741 TGGCGGCGTC CATGGATTCA AGCTCACAGC CCGGCGCAAT GTATGCATCT CTCTCTCTCT
1801 CTCTCTCTCT CTCTCTCTCT CTCTCTCTCT CTCTCTCTCT CTCTCTCTCT CTACATCATC
1861 GTTTGGGGGA TGAATCAAAT GGGGCTGCCA ATTATCAAGG AATGAATGGT TTTTGTTCAC
1921 CCTCCTTATA TTAGTCTTTC TCTCTACGCT GTGTTTGGTG CGTTTGCCTT AAACCACACT
1981 CGGTGTATTA GGGGTTGGCA ACTTATCATA GCTTTGGTTC TCATGCATGC ATGTATGGTT
2041 CATCATGTTT TTGTCAAATT TTCATGTAGC AACATATTGT CCTCCGTCCA CAACAGATAA
2101 GCTGATCCTG CTAGTCATAG CTGCTATATA CAGATCAGCT TATTAAGTTT GCATCATTGT
2161 AGAAGCAAAA GTAATTAAGC ACCCGGGCGG CAGACATGTT ACGTACGTAT ATAACAGGTT
2221 GTTGTTATGC GTGTTCTAAT GTTCCTTGGC ACAACAACTG TAGTGATACA TGCAGAGGGA
2281 GCGGAGGAGG AGGAGATAGA AACCAAAGGG AGGAGGACGC GGCGGCGGCG GCGGCGGCAG
2341 AGGCCGGCTA CGGCAGGCAG CTGGTGATTC CCGAGGACGG GTACGAGTGG AAGAAGTACG
2401 GCCAGAAGTT CATCAAGAAC ATCCAGAAAA TCAGGTACTT GCTCCGTTCG ATCCAACATA
2461 TGCATACGTA GCATTTTTGG CATCGAGATT GATCTCGAGC TCTCAAATAA AGCTAGTGCA
2521 AACTTGATCA CATATACCAT TTTTTCGTGG TCAAATCTCG TTTCCCGCCA TACGCGTGTA
2581 CATCAGATTA ATCAATAGCT CGACGTTGAC CAAGCTTGTT GACTTGTTCA TCTTCGTTCC
2641 TGTGCATCAA ATCGTTTTAT TAATTAAWTG AGTCGATGTG ACGCCCATCG ATCGATCACT
2701 GGTATAATGG AATGTATGGG TTGCCCGCCG TCCCCGTGCA TATATGCATA CGTGCAATGC
2761 TCTGCTGCCA GATCTTATCT TTCGAAGAAG AATCAACGGA AGAATAATAT CCTCGCTTTA
2821 TTATATTATA TATTGATAAC GGTCGACCAA ATAAAGCCCT GATGATGACT TGATGAGCAA
2881 ACTGCACAAG TGTGTTTTGC ATTGCATGCC AACTGATGAT ACCACCGTAC GTGGGTGGTC
2941 CATGATGCAT GTGTGTGATC AAAATCCAAC AATGGCGCAG GAGCTACTTC CGGTGTCGGC
3001 ACAAGCTGTG CGGCGCCAAG AAGAAGGTGG AGTGGCACCC GCGGGACCCC AGCGGCGACC
3061 TCCGCATCGT CTACGAGGGC GCGCACCAGC ACGGCGCCCC GGCGGCGGCG GCTCCTCCCG
3121 GTCCCGGCGG CCAGCATCAG GGCGGCGGCG CCTCCGACTT CAACAGATAC GAGCTGGGCG
3181 CGCAGTACTT CGGCGGGGCC GGCCGGTCGC ATTGACGCGG GGCGCTAGTT CCTAAAATAT
3241 TTTGTAAAAT TTTTCACATT CTCGTCACAT CAAATTTTGC GGCACATATA TATATATATA
3301 GAGTACTAAA TATATATAAA AAAATAACTA ATTACATAGT TTACCTATAA TTTATGAGAC
3361 GAATCTTTTG ATCCTAGTTA GTCAATAATT AACAATATTT GTTAAATACA AACAAAATTA
3421 TTACTATTCC TATTTTA
>SpTATA-construct
1 CCTGTTTGGA TCCACTATGG TTATAATAAT TAGCTAAAAT AAGTTAATTA TGAACTAAAC
61 ATAACTAAAA CACTCGATTA TATATAATCT CTCTCTCTTA CCAGCTTTTT GGTGACCATG
121 ATTTCCCACT CGAACGAAAA TGATCCATCA CAATAATTCA AACAGTCCGA TTAGTATAGT
181 TCATCATTCA TCATTTCATA ATTCATAATA ATTTTCTTTC AAAGAACCAT AGTACGATCT
241 TCTCGGTGCG TTCGTAACCT GCTAGTGCGA TTTGCGAGCA GGAGAAATTC TTGATTAATG
301 GCCGTCGAAC GCGCATCATA CCCACACACC GACACCGGGC CCCACCGCAT GCTATGACCC
361 ACAGGTCAGT TTGTATAACT CGAACCAGCG CGTGGGCAAA CCGCGCCCTG TGGTAACCGG
421 CCACAGCCGG TGCACCCTGT TCCCCTACAA AAGCCAAGCA CTCGCCATCC ACCGCCTGCT
481 CCCCGTCCAC CGATACAACC CCAACTCCCG CACGCATCTA CCTCCCACCA CCAGACGACC
541 TCCCCCTCTT CCCCCCGCAT TCTCACGCCG CCCGCGCTGT CCCCGACTCC CCCTCGCGAG
601 ACAGATAACC CTAGCTCTCC TTCCCCTTGC TTTCCCTCCT CCCCAACTCC TCTCCCGATC
661 TCTATAAAAG ACCCGGATTT CGAATTCCCC CTCGAACCGG TGACGCGCGC GCTCCTTTCC
721 CGCCCATTTC GGCCCCGGAT TGAGCCCGGA TTCGCGGCGC GGAGCCCCGC CGAGGTCGCG
781 TGCGTGGTTC GGATGGCGGA GCCGGGGCTC GAGGGCAGCC AGCCGGTGGA TCTGTCCAAG
841 CACCCCTCCG GCATCGTCCC CACGCTCCAG TAAGTCCCGG ATCTGGGTCG TCGGGGATTC
901 GGGGTTCCGG AGAGATCCCG TACCGTACGT GCTAGGGTCC CGCCGCCCGG CGGATGGGTG
961 CTTGGGTGTG CTGTGGAGCT GCCAAGGCTT GGTTCTGGCC AGCGGGGTGC CTCCCTGTCT
1021 TGTGGGTGGT GGTGTGGTTG GTTTATGCCG TGTTTTTGTT CAGCTTCCCA GTGCTGGGTG
1081 CATCGAAACG AGCTAATATT GTCTTAAATA GTTGGGGCTT TGGTGGGAAT TATTTAGTTG
1141 TCATCCTCTC TCCGAGGGTT GATTTCAGGT ATGAAACTTG GGAGAACGAG AGAGGGCCAA
1201 ACTGGAGAGG AGATCTATTT CCTTATTGAA GTTTGTCATC TTGGCGTAAT AGTTGCAGCA
1261 AGCATAATCT CTTCTTACCA TATTTCCATT CCATGTTTTG ACTGGTTGGT GCATAAATTG
1321 TGTAATATGA TAAACAGTCA GCCTCACGGA CTGAGGTTTC GATATCTCAT ACCCTGGGGA
1381 GGGTTAAATG CATTGGTTTG GATTGAGGGA CGTTCCAGAC GGGATGGGCC AATCCTTTTT
1441 CAGCAGTGTT TGGTTGGGAG GAGCTAGGGA TGGGGTCATT TTTGCAGTGG CATATTCCTG
1501 AAATACTTGG ACAGCTCGGT CCCCTTGAAG CGAGTGGATG AAGCCATTCC TTGCGGACCG
1561 TGTTGCACGC CATCTTTGCA TTGCCTGGTG CAAGCGTGGT CAAAAGGAGG GGCGCGGGCA
1621 TGTGGAAGCT CCAAGCTCAA GGCAACCAGA GGAGAGGAGA TGTTTGGAGA ATATGCAGCG
1681 CACTGGGTTA GCGCCGACGA TATATGAGAG GAGTGGGGTG AGCTCCAGCA TGGGAAGGAG
1741 CGGGTCCAAT TACCTCCTCT GGCCACATGA GATCTCTGGT GGGGCAGGTT GAGCATCTCT
1801 GATGGTGGCA ATGCCAAGAT CTCTGCATGA GATCTTGGGC GAATGGGTTG GCCAAGCTCT
1861 TGGGCGGTGG TAGTGCCCAG CTGTTGGGCA CCGGAGCTGT GAGATGTTGA GTGACTGTGG
1921 TACAAAATCT TGGGAATCAG GCTTTGCTCA CCCGAAGAAG ACAACAAGAA TAGGTGTAGA
1981 GACAATGGCT GGTGCGGCCA TGGATTATTG TGTCTAATGG AGTTAAAACT CCAGCGGGTT
2041 CCATCCCACC ATATCCTTAG AACCAAACAA AAATTGGAAT GAACCTATCC GTCCAACCAA
2101 ACAAGGTCTG GGTCAATCCC GTCCAAAAAA TAGGGACATG ACCATTCCAT CCCACTCCGT
2161 CCCCAAACCA GAAGTAACCT AACTAGCTTT TGTGGAAGAT TTTTAGTATG ATTTAGGTGT
2221 AAGATTTTTC TGCAGGTGAT TTCATTATTA AGTGCTATGG GCTTATTAGT GCCATATTGA
2281 GTCCTGAAAT GACTTTGATT GATACAAAGA TTTTATGATC CAACCATTAT AGGGTAGGTT
2341 ATCTTGAATG TTAGTCTTAA ATTTGTTATG ATTGTACATG TTGGAAATTG GCATGAAGAA
2401 GAAATTAATA GCACACACTA TCATATAAAA AAAGAACAAT GAAAATCATC TTGTAGTGTC
2461 TCTTCAATGC TAAAAACTTA TAATAAGAAC CCTATGTTGG ATTTACAAAA CAAAATTCAC
2521 ATGTTTACTG CAGTTCTTTG ATCATGGTCT GTTTGACGTG CCATTTTTTC ATGACAGGAA
2581 TATTGTATCA ACAGTTAATT TGGATTGTAA ACTTGACCTC AAAGCAATAG CTTTGCAAGC
2641 ACGAAATGCG GAGTATAACC CAAAGGTATA GTGATTACCT CAAAGCAATG GCTGCACGGT
2701 ATTTACTGGT GGTTTTATCT AGTGGAAGAT TTATGGATAT ATAAAGTATG TTGATCCTTT
2761 GTAATGATGT ACAATCTTGT CCATGAGTTC AAATAGCTTT GTGCTAATAA CTAATGGATG
2821 GCCTGGGATA TGAGATGCGC ACGGTTAGTT TTTAACCAGA TGGATGCTCA TATCCTGCTT
2881 GTCCTGTGAA TAACCACAAT ATTCTTTGAC AAACAAGTTC TAAATTTTTA GCTTATATAT
2941 GACTTGTTTG CTCTTTTAGG CATTCTATTG AATGCAATCT TTCTCAGTTA TGATGATACA
3001 GTGTAGCAAC CTGAATTTTG CTAAGTTTTC TGTCATTATA TTTCGTATTT GACTTTTTTA
3061 TCTGTAAACA GCGTTTTGCT GCAGTCATCA TGAGAATAAG GGAACCCAAA ACCACAGCAC
3121 TGATATTTGC ATCGGGTAAA ATGGTATGTC ATCAACATCT TTTGTCTTAT ACTGTCAATT
3181 TTCAAGGATT TTGCATGTTT TTTATGTAAA CAGATTTGAT CTTTTTTTTT TGACATGTTT
3241 TGTTGCCTTG GTGCTGTGCT GTCAAAAGAT AGAAAGGCTA ATCTTTTTGT TTTTCTGTGT
3301 CCTAATCTAC CTCTCTCAAT CATCAAATAT TGGAGAATAA AGTTCTTACC CTCTCTAGCC
3361 TACCCTAGGC AAATCTATTT TTCCCTTACC AGATGATATA TGTCGGTGGG TTTTTTACTT
3421 GGGATTTTAT TTGGATCAAT GTTCAGTATT GTAGAGTTTC TTGGTTGTCA TATTCAATAT
3481 TAATTAAATG TATTGATGTG TCAAATACTT TTCTTCCAAA TGAAGAAAGT TTACTAGAAC
3541 TGCATTTCTT TCATATAGAA CTACAATTTT GTATCAATCC ATCATAAGTG TTCTAGTGCT
3601 GAAGCAAGCA ACCGATAATT TTCTATGACC AGGTATGTAC TGGAGCAAAG AGTGAACAGC
3661 AATCTAAGCT TGCAGCAAGA AAGGTGCCAT ATGGCTTGCT ACCATTTTAT TTAAGTTGGA
3721 TCTAGTTTAC TGAGAGGCTT TGGAATTCTA ACCCCTGTCT GTATTTCCAG TATGCTCGTA
3781 TCATTCAGAA ACTAGGTTTT CCTGCTAAAT TTAAGGTAAC TATTTTCATA CTTGTGTACA
3841 TTGACCTGTA CTTTGAACAA TTTGTACATC AGTTGCTAAG TTACTGTTCT ATGCCAGGAC
3901 TTTAAGATTC AGAATATTGT TGGCTCTTGT GATGTCAAGT TTCCAATTAG GCTTGAGGGC
3961 CTTGCATATT CTCATGGTGC CTTCTCAAGT GTATGTCCAA TTATGGCTTA TTTTAACATC
4021 ACAGTAATTC TTTTACCCTT TTTTGTCTGC ATTCTTTGGA TAATGACATT TTTAATTTGT
4081 TTTACAGTAC GAACCAGAAC TCTTTCCTGG CCTTATCTAT CGGATGAAAC AACCAAAGAT
4141 TGTTCTTTTA ATTTTTGTTT CAGGCAAGAT TGTTTTGACT GGAGCAAAGG TTAGTTGTCA
4201 TTTCGTGCTC ATGCTTAATT ATACTGCGAT TTGCTTATCT TTCTCCTTCA TTTCATAGTC
4261 GATTGTTTGT AGGTGAGAGA GGAGACTTAC ACTGCCTTCG AGAACATCTA TCCTGTACTG
4321 ACAGAGTTTA GAAAAGTTCA GCAATGGTAT GTAGTTCTTT TTATCATGCT TCAGTAGTGC
4381 ATGCATGACT CTTCCTACAA TTGAGATTCT CTTGTTCTGT TGACTATGTT CACTATGTCT
4441 TGTACTCATT TTGGAACATG TGTGGGACTG GGCACCTATA TGTTATTTGT TGTTAGACTT
4501 GATAGTAAAA TGTTGGAACC ATGTTTTCTA CTGTGTTTTC GAAACAAACT TTTATTATGG
4561 TATTCTAGTT ATTCAAATCA GATGTCATGG CATGTAAATA GCTGCCTGAG GGGATAATTC
4621 CTTCTCTCGT TAAGCCCCTT TTGGAATGTG GGATCCCCCT GTTTCCTGTG TTTTCCTGTG
4681 AAAATGGACC GATTCCCTTG GAATTGTACA TGATTTCTGT GAAATTTCTG TATTCCAAAT
4741 GGATTGTTTA TATTGTTTAT AACTCGTGTC GGTGTGTGCA GTACTAGAAT TAACTATTTA
4801 AAACTAATCT TTTCCTTCTG GTTCCCTCTT TGCAGATGAC CCAAGTGAAG GTCACGTTCT
4861 TCTAATTACA AGCATCCTTG AGGTTTTTTG TGTGACCATA AATTCTAACT GTACATAAAA
4921 AGTCTGGCGG AACTTATAGC ATCCTGTAAT TTCTGTGAGC CTCGGTGTGT CATGGTTGGG
4981 TGGGTTTGTT GTGGTAGCTG ATGGACTTGT CAGATTTGTC CATTTGGGTA GGCCCTGGTG
5041 AACACGGACA CCTATCATGC TTCAGTTTGA GTAGTTTGTG TCCCATGTGG TTAACTTGAG
5101 TGCTAACAAG AGTAGATAAA TTTTGCCCTA CTTTGTCTTT GAGACACGAG TCTATTAAAG
5161 TCTTTTCTGA GTATCTTTCA GTGCTGTGCG ATGATGAAAA GGCTGGCACT GTAGGTAACT
5221 GGGGCAATGT ACTGACTACT GACGGGACTG TGTGACTGGT TGTAAGTTAT CTGTTGTATT
5281 TGAGATGCTG TATCGTAGAG GACACCGGGA ATAATCTTGT TGGTTATCAC TGTATGGAGG
5341 AACCGGTCAG TCGACCACGC AGTAGACTTG TTTCTGTATC CAAATGTACC TGCACTTGGT
5401 TCTTTTACTT TTTCGATGTT GCATGCTAGG ATGAATAATT TGAATTCTAC GCACTGTATA
5461 AGAATGCCTA TTAACGTATC TGGAAAGATT GCTCGGCGAA CGAGCTGTCT TGTATCGCAT
5521 GGAACTGTGT TTGCCGTTTT CCTCAATCGA AAACATTTTA TTAATGTTGC CAACTTGTTG
5581 GTAATGGATG CCCAGCCGTT GCAGTGATCA GTTGTCGGCA GAGGTTAGGG GATACAGTGC
5641 GGCGTTCGTT ACCCATTGAT GCCTTGCTAT CTACAAATGA TTGAATGTTA AAGCTGACTA
5701 AACGAATTAG GTTTATCCCA CTGATAAAGC TAGGTCTCGA GTGTCGCATT CAGTTCAGAG
5761 GCGATGCAAT GTAAGCCCGG GTGACCAGGG CCTCGAACCC AACTCTGGAG ACTTTCGACT
5821 ACTAGAGCAC AAGTTTACAC TTTAGAGTTG CAAAAAACAA AACATAATAG CGACGGTGCT
5881 AGTTTCCACT TTCAGCTAAA TATTTTTTTA TCCATATACA ATATGAAAAT TACAATTATG
5941 CACCATCGTC CTAATAGTAC CGTACACTAG AACTAGACCA GAGAGTTAAC CGGAGCAGCA
6001 ACAATTAGTC CGCACTCAAC ATCATGTTGC GCCGGCGCAA GGCGTCAACC GGCACCGGCA
6061 GCACCTGCCG TTGCCCTCCG GTGCGCAGCG AAAGCGATCG ATCGATGCAG CGGCAGCCAG
6121 CAGAGGCCAG CACGTACAGG AGCTGATGCA TGCGTACGTG ACGCAACGCC GCCGGCCTTT
6181 CTTGTCGGTT TGTCAACCTA GG