ENZYME WEBQUEST

#1: Mrs. Carreno’s teacher page – Basic enzymes powerpoint

  1. SLIDE TWO: Enzymes are ______that serve as ______.

They ______up or ______reactions, but ALWAYS remain ______.

  1. SLIDE THREE: What is an ACTIVE SITE?
  1. What is a SUBSTRATE?
  1. SLIDE FOUR: Each ______acts on a specific______.
  1. DRAW an ENZYME and SUBSTRATE below. Make each a DIFFERENT COLOR and label each.
  1. SLIDE FIVE: What is the INDUCED FIT?
  1. SLIDE EIGHT: YOU JUST DRANK A GLASS OF MILK! DRAW THE CATALYTIC CYCLE OF LACTOSE BELOW! LABELING THE SUBSTRATE, SUBSTRATE ENZYME COMPLEX, THE ACTIVE SITE, AND THE PRODUCTS. MAKING EACH A DIFFERENT COLOR!
  1. Someone who is “LACTOSE INTOLERANT”…is lacking which enzyme?______

WEBSITE #2:

Enzyme Catalysis

Introduction

  1. ______catalyze reactions by lowering the
  1. ______necessary for a reaction to occur.

Key Concepts

  1. ______catalyze reactions by lowering the ______necessary for a reaction to occur. The molecule that an enzyme acts on is called the ______. In an enzyme-mediated reaction, ______molecules are changed, and ______is formed. The ______molecule is ______after the reaction, and it can continue ______.
  2. Each ______is ______for the ______it will catalyze. In this laboratory,

Enzyme = catalase, found in your liver

Substrate = hydrogen peroxide (H2O2)

Products = water and oxygen

  1. DRAW THE REACTION THAT TOOK PLACE ON THE LEFT USING YOUR OWN SHAPES, LABELING THE SUBSTRATE, ENZYME, ACTIVE SITE ON THE ENZYME, ENZYME-SUBSTRATE COMPLEX, AND THE PRODUCTS.

Enzyme Structure

  1. Enzymes are globular ______. Their folded conformation creates an area known as the ______. The nature and arrangement of ______in the ______make it specific______.
  1. DRAW A MODEL OF AN ENZYME, ACTIVE SITE, AND SUBSTRATE BELOW:

Binding Specificity

  1. Even when different ______molecules are present, only those that have the ______to the ______are able to bind with the enzyme's ______.

Induced Fit

  1. Observe the INDUCED FIT ANIMATION and describe what happens below:

Some Factors That Affect Enzyme Action

  1. The conformation of an enzyme is maintained by interactions between the various ______that compose it, and this conformation is sensitive to ______. Two important influences are ______and ______. When an enzyme's ______is significantly altered because of ______or ______variation, the enzyme may no longer ______. An enzyme is said to be ______when it loses its functional shape.

pH and Enzyme Function

  1. Each enzyme functions best within a ______. For example, the enzyme______, which works in your stomach, functions best in a strongly ______environment. Lipase, an enzyme found in your ______, works best in a ______environment.
  2. When the pH changes, the active site ______and affects ______. What happens to catalysis when an enzyme is subjected to a pH far from its optimum range?
  1. WATCH THE ANIMATION AND DESCRIBE WHAT YOU SEE BELOW:

Temperature and Enzyme Function

  1. Chemical reactions speed up as ______is increased, so, in general,______will ______at higher temperatures. However, each enzyme has a temperature ______, and beyond this point the enzyme's ______is lost. ______temperatures will ______most enzymes.

WEBSITE #3:

  1. Click on BIOCHEMICAL PATHWAY, MAKE SURE YOUR VOLUME IS TURNED UP…what A BIOCHEMICAL PATHWAY?

Now click on Feeback Inhibition

  1. What is Feedback Inhibition?
  1. What is an ALLOSTERIC SITE?
  1. Why might this process take place?

WEBSITE #4: THIS is DIFFICULT…but you need to get the GENERAL IDEA! MAKE SURE YOUR VOLUME IT UP….SO YOU CAN HEAR THE SITE!

  1. HOW ENZYME INHIBITORS ACT AS DRUGS!

CASE I: HOW ASPIRIN AND OTHER NONSTEROIDAL ANTI-INFLAMMATORY INHIBITORS WORKS

Nonsteroidal anti-inflammatory drugs work by interfering with the cyclooxygenase pathway. The normal process begins with arachidonic acid, a dietary unsaturated fatty acid obtained from animal fats. This acid is converted by the enzyme cyclooxygenase to synthesize different prostaglandins. The prostaglandins go on to stimulate many other regulatory functions and reactionary responses in the body including: inflammation and increased sensitivity to pain . Aspirin and other NSAID's work by inhibiting this pathway.

  1. EXPLAIN IN YOUR OWN WORDS….HOW ASPRIN BLOCKS INFLAMMATION!

WEBSITE #9:

  1. Explain what an enzyme inhibitor is.
  1. Enzyme inhibitors occur naturally in your cells. Explain how enzyme inhibitors maintain homeostasis in your body.