ESM 2: Two Way Analysis of Variance on soil metal content (total or available) using the treatment and the plant group as fixed effects.
1) Dependent Variable: Zn total concentration in soil
All Pairwise Multiple Comparison Procedures (Holm-Sidak method):
Overall significance level = 0.05
Comparisons for factor: Treatment
Comparison Diff of Means t P P<0.050
BC-EDDS vs. BC-EDTA 108.244 46.304 <0.001 Yes
EDTA vs. BC-EDTA 87.562 37.457 <0.001 Yes
EDDS vs. BC-EDTA 86.182 36.867 <0.001 Yes
BC vs. BC-EDTA 71.215 30.464 <0.001 Yes
HM vs. BC-EDTA 58.928 25.208 <0.001 Yes
BC-EDDS vs. HM 49.315 21.096 <0.001 Yes
BC-EDDS vs. BC 37.028 15.840 <0.001 Yes
EDTA vs. HM 28.633 12.249 <0.001 Yes
EDDS vs. HM 27.254 11.658 <0.001 Yes
BC-EDDS vs. EDDS 22.061 9.437 <0.001 Yes
BC-EDDS vs. EDTA 20.682 8.847 <0.001 Yes
EDTA vs. BC 16.346 6.993 <0.001 Yes
EDDS vs. BC 14.967 6.402 <0.001 Yes
BC vs. HM 12.287 5.256 <0.001 Yes
EDTA vs. EDDS 1.380 0.590 0.559 No
Comparisons for factor: Treatment within First sampling
Comparison Diff of Means t P P<0.05
EDDS vs. BC-EDDS 6.849 1.692 0.792 No
HM vs. BC-EDDS 5.457 1.348 0.944 No
EDTA vs. BC-EDDS 4.016 0.992 0.994 No
BC vs. BC-EDDS 3.857 0.953 0.994 No
EDDS vs. BC-EDTA 3.748 0.926 0.993 No
BC-EDTA vs. BC-EDDS 3.101 0.766 0.997 No
EDDS vs. BC 2.992 0.739 0.996 No
EDDS vs. EDTA 2.833 0.700 0.995 No
HM vs. BC-EDTA 2.356 0.582 0.997 No
HM vs. BC 1.600 0.395 0.999 No
HM vs. EDTA 1.441 0.356 0.998 No
EDDS vs. HM 1.392 0.344 0.995 No
EDTA vs. BC-EDTA 0.915 0.226 0.994 No
BC vs. BC-EDTA 0.756 0.187 0.978 No
EDTA vs. BC 0.159 0.0392 0.969 No
Comparisons for factor: Treatment within Second sampling
Comparison Diff of Means t P P<0.05
BC-EDDS vs. BC-EDTA 136.407 33.689 <0.001 Yes
EDTA vs. BC-EDTA 98.633 24.360 <0.001 Yes
BC-EDDS vs. HM 98.068 24.220 <0.001 Yes
EDDS vs. BC-EDTA 75.541 18.657 <0.001 Yes
BC-EDDS vs. BC 71.323 17.615 <0.001 Yes
BC vs. BC-EDTA 65.084 16.074 <0.001 Yes
BC-EDDS vs. EDDS 60.865 15.032 <0.001 Yes
EDTA vs. HM 60.294 14.891 <0.001 Yes
HM vs. BC-EDTA 38.339 9.469 <0.001 Yes
BC-EDDS vs. EDTA 37.774 9.329 <0.001 Yes
EDDS vs. HM 37.202 9.188 <0.001 Yes
EDTA vs. BC 33.549 8.286 <0.001 Yes
BC vs. HM 26.745 6.605 <0.001 Yes
EDTA vs. EDDS 23.092 5.703 <0.001 Yes
EDDS vs. BC 10.457 2.583 0.014 Yes
Comparisons for factor: Treatment within Third sampling
Comparison Diff of Means t P P<0.05
BC-EDDS vs. BC-EDTA 191.425 47.277 <0.001 Yes
EDDS vs. BC-EDTA 179.257 44.272 <0.001 Yes
EDTA vs. BC-EDTA 163.137 40.291 <0.001 Yes
BC vs. BC-EDTA 147.806 36.504 <0.001 Yes
HM vs. BC-EDTA 136.090 33.611 <0.001 Yes
BC-EDDS vs. HM 55.335 13.666 <0.001 Yes
BC-EDDS vs. BC 43.619 10.773 <0.001 Yes
EDDS vs. HM 43.167 10.661 <0.001 Yes
EDDS vs. BC 31.451 7.768 <0.001 Yes
BC-EDDS vs. EDTA 28.288 6.986 <0.001 Yes
EDTA vs. HM 27.047 6.680 <0.001 Yes
EDDS vs. EDTA 16.120 3.981 0.001 Yes
EDTA vs. BC 15.331 3.786 0.002 Yes
BC-EDDS vs. EDDS 12.168 3.005 0.010 Yes
BC vs. HM 11.716 2.894 0.006 Yes
2) Dependent Variable: Cu total concentration in soil
All Pairwise Multiple Comparison Procedures (Holm-Sidak method):
Overall significance level = 0.05
Comparisons for factor: Treatment
Comparison Diff of Means t P P<0.050
BC-EDDS vs. BC-EDTA 58.692 56.598 <0.001 Yes
BC vs. BC-EDTA 55.261 53.289 <0.001 Yes
EDDS vs. BC-EDTA 51.377 49.544 <0.001 Yes
EDTA vs. BC-EDTA 47.569 45.872 <0.001 Yes
HM vs. BC-EDTA 36.653 35.345 <0.001 Yes
BC-EDDS vs. HM 22.039 21.253 <0.001 Yes
BC vs. HM 18.608 17.944 <0.001 Yes
EDDS vs. HM 14.724 14.199 <0.001 Yes
BC-EDDS vs. EDTA 11.123 10.726 <0.001 Yes
EDTA vs. HM 10.916 10.527 <0.001 Yes
BC vs. EDTA 7.692 7.417 <0.001 Yes
BC-EDDS vs. EDDS 7.315 7.054 <0.001 Yes
BC vs. EDDS 3.884 3.745 0.002 Yes
EDDS vs. EDTA 3.808 3.672 0.002 Yes
BC-EDDS vs. BC 3.431 3.309 0.002 Yes
Comparisons for factor: Treatment within First sampling
Comparison Diff of Means t P P<0.05
BC vs. EDTA 10.062 5.602 <0.001 Yes
BC vs. EDDS 8.868 4.937 <0.001 Yes
BC-EDDS vs. EDTA 8.843 4.923 <0.001 Yes
BC vs. HM 7.894 4.395 0.001 Yes
BC-EDDS vs. EDDS 7.649 4.259 0.002 Yes
BC-EDDS vs. HM 6.676 3.717 0.007 Yes
BC vs. BC-EDTA 5.843 3.253 0.022 Yes
BC-EDDS vs. BC-EDTA 4.625 2.575 0.109 No
BC-EDTA vs. EDTA 4.219 2.349 0.159 No
BC-EDTA vs. EDDS 3.025 1.684 0.471 No
HM vs. EDTA 2.168 1.207 0.739 No
BC-EDTA vs. HM 2.051 1.142 0.702 No
BC vs. BC-EDDS 1.219 0.679 0.876 No
EDDS vs. EDTA 1.194 0.665 0.760 No
HM vs. EDDS 0.974 0.542 0.591 No
Comparisons for factor: Treatment within Second sampling
Comparison Diff of Means t P P<0.05
BC-EDDS vs. BC-EDTA 108.282 60.286 <0.001 Yes
BC vs. BC-EDTA 87.404 48.662 <0.001 Yes
EDTA vs. BC-EDTA 76.816 42.767 <0.001 Yes
EDDS vs. BC-EDTA 75.906 42.261 <0.001 Yes
HM vs. BC-EDTA 58.439 32.536 <0.001 Yes
BC-EDDS vs. HM 49.843 27.750 <0.001 Yes
BC-EDDS vs. EDDS 32.376 18.025 <0.001 Yes
BC-EDDS vs. EDTA 31.466 17.519 <0.001 Yes
BC vs. HM 28.966 16.127 <0.001 Yes
BC-EDDS vs. BC 20.877 11.623 <0.001 Yes
EDTA vs. HM 18.377 10.231 <0.001 Yes
EDDS vs. HM 17.467 9.725 <0.001 Yes
BC vs. EDDS 11.498 6.402 <0.001 Yes
BC vs. EDTA 10.589 5.895 <0.001 Yes
EDTA vs. EDDS 0.910 0.506 0.616 No
Comparisons for factor: Treatment within Third sampling
Comparison Diff of Means t P P<0.05
EDDS vs. BC-EDTA 81.250 45.236 <0.001 Yes
BC vs. BC-EDTA 72.535 40.384 <0.001 Yes
EDTA vs. BC-EDTA 70.111 39.034 <0.001 Yes
BC-EDDS vs. BC-EDTA 63.171 35.170 <0.001 Yes
HM vs. BC-EDTA 53.572 29.826 <0.001 Yes
EDDS vs. HM 27.678 15.410 <0.001 Yes
BC vs. HM 18.963 10.558 <0.001 Yes
EDDS vs. BC-EDDS 18.080 10.066 <0.001 Yes
EDTA vs. HM 16.539 9.208 <0.001 Yes
EDDS vs. EDTA 11.140 6.202 <0.001 Yes
BC-EDDS vs. HM 9.599 5.344 <0.001 Yes
BC vs. BC-EDDS 9.364 5.213 <0.001 Yes
EDDS vs. BC 8.716 4.852 <0.001 Yes
EDTA vs. BC-EDDS 6.940 3.864 <0.001 Yes
BC vs. EDTA 2.424 1.350 0.186 No
3) Dependent Variable: Zn available content in soil
All Pairwise Multiple Comparison Procedures (Holm-Sidak method):
Overall significance level = 0.05
Comparisons for factor: Treatment
Comparison Diff of Means t P P<0.050
EDTA vs. EDDS 0.0163 10.685 <0.001 Yes
BC-EDTA vs. EDDS 0.0137 8.932 <0.001 Yes
EDTA vs. BC-EDDS 0.0121 7.182 <0.001 Yes
BC vs. EDDS 0.0119 7.059 <0.001 Yes
EDTA vs. HM 0.0118 7.017 <0.001 Yes
BC-EDTA vs. BC-EDDS 0.00942 5.591 <0.001 Yes
BC-EDTA vs. HM 0.00914 5.427 <0.001 Yes
BC vs. BC-EDDS 0.00766 4.190 0.002 Yes
BC vs. HM 0.00738 4.038 0.003 Yes
HM vs. EDDS 0.00451 2.679 0.072 No
EDTA vs. BC 0.00444 2.637 0.067 No
BC-EDDS vs. EDDS 0.00424 2.515 0.071 No
EDTA vs. BC-EDTA 0.00268 1.753 0.249 No
BC-EDTA vs. BC 0.00176 1.046 0.516 No
HM vs. BC-EDDS 0.000277 0.152 0.881 No
Comparisons for factor: Treatment within First sampling
Comparison Diff of Means t P P<0.05
BC vs. EDDS 0.00464 1.261 0.975 No
HM vs. EDDS 0.00464 1.261 0.968 No
BC vs. BC-EDDS 0.00480 1.130 0.983 No
HM vs. BC-EDDS 0.00480 1.130 0.976 No
EDTA vs. EDDS 0.00280 0.932 0.993 No
BC vs. BC-EDTA 0.00325 0.883 0.992 No
HM vs. BC-EDTA 0.00325 0.883 0.987 No
EDTA vs. BC-EDDS 0.00296 0.804 0.989 No
BC vs. EDTA 0.00184 0.501 0.999 No
HM vs. EDTA 0.00184 0.501 0.997 No
EDTA vs. BC-EDTA 0.00140 0.468 0.994 No
BC-EDTA vs. EDDS 0.00139 0.464 0.984 No
BC-EDTA vs. BC-EDDS 0.00155 0.422 0.966 No
EDDS vs. BC-EDDS 0.000160 0.0436 0.999 No
BC vs. HM 3.469E-018 8.172E-016 1.000 No
Comparisons for factor: Treatment within Second sampling
Comparison Diff of Means t P P<0.05
EDTA vs. HM 0.0170 6.949 <0.001 Yes
BC-EDDS vs. HM 0.0136 5.562 <0.001 Yes
BC-EDTA vs. HM 0.0136 5.548 <0.001 Yes
EDTA vs. EDDS 0.0123 5.004 <0.001 Yes
BC vs. HM 0.00957 3.903 0.006 Yes
BC-EDDS vs. EDDS 0.00887 3.617 0.012 Yes
BC-EDTA vs. EDDS 0.00883 3.604 0.011 Yes
EDTA vs. BC 0.00747 3.046 0.040 Yes
BC vs. EDDS 0.00480 1.958 0.354 No
EDDS vs. HM 0.00477 1.945 0.320 No
BC-EDDS vs. BC 0.00407 1.659 0.437 No
BC-EDTA vs. BC 0.00403 1.645 0.377 No
EDTA vs. BC-EDTA 0.00343 1.401 0.434 No
EDTA vs. BC-EDDS 0.00340 1.387 0.322 No
BC-EDDS vs. BC-EDTA 0.0000333 0.0136 0.989 No
Comparisons for factor: Treatment within Third sampling
Comparison Diff of Means t P P<0.05
EDTA vs. EDDS 0.0339 13.844 <0.001 Yes
BC-EDTA vs. EDDS 0.0307 12.538 <0.001 Yes
EDTA vs. BC-EDDS 0.0299 12.212 <0.001 Yes
BC-EDTA vs. BC-EDDS 0.0267 10.906 <0.001 Yes
BC vs. EDDS 0.0262 10.702 <0.001 Yes
BC vs. BC-EDDS 0.0222 9.071 <0.001 Yes
EDTA vs. HM 0.0203 8.268 <0.001 Yes
BC-EDTA vs. HM 0.0171 6.963 <0.001 Yes
HM vs. EDDS 0.0137 5.576 <0.001 Yes
BC vs. HM 0.0126 5.127 <0.001 Yes
HM vs. BC-EDDS 0.00967 3.944 0.003 Yes
EDTA vs. BC 0.00770 3.141 0.016 Yes
BC-EDTA vs. BC 0.00450 1.836 0.215 No
BC-EDDS vs. EDDS 0.00400 1.632 0.216 No
EDTA vs. BC-EDTA 0.00320 1.306 0.203 No
4) Dependent Variable: Zn available content in soil
All Pairwise Multiple Comparison Procedures (Holm-Sidak method):
Overall significance level = 0.05
Comparisons for factor: Treatment
Comparison Diff of Means t P P<0.050
EDTA vs. EDDS 0.0163 10.685 <0.001 Yes
BC-EDTA vs. EDDS 0.0137 8.932 <0.001 Yes
EDTA vs. BC-EDDS 0.0121 7.182 <0.001 Yes
BC vs. EDDS 0.0119 7.059 <0.001 Yes
EDTA vs. HM 0.0118 7.017 <0.001 Yes
BC-EDTA vs. BC-EDDS 0.00942 5.591 <0.001 Yes
BC-EDTA vs. HM 0.00914 5.427 <0.001 Yes
BC vs. BC-EDDS 0.00766 4.190 0.002 Yes
BC vs. HM 0.00738 4.038 0.003 Yes
HM vs. EDDS 0.00451 2.679 0.072 No
EDTA vs. BC 0.00444 2.637 0.067 No
BC-EDDS vs. EDDS 0.00424 2.515 0.071 No
EDTA vs. BC-EDTA 0.00268 1.753 0.249 No
BC-EDTA vs. BC 0.00176 1.046 0.516 No
HM vs. BC-EDDS 0.000277 0.152 0.881 No
Comparisons for factor: Treatment within First sampling
Comparison Diff of Means t P P<0.05
BC vs. EDDS 0.00464 1.261 0.975 No
HM vs. EDDS 0.00464 1.261 0.968 No
BC vs. BC-EDDS 0.00480 1.130 0.983 No
HM vs. BC-EDDS 0.00480 1.130 0.976 No
EDTA vs. EDDS 0.00280 0.932 0.993 No
BC vs. BC-EDTA 0.00325 0.883 0.992 No
HM vs. BC-EDTA 0.00325 0.883 0.987 No
EDTA vs. BC-EDDS 0.00296 0.804 0.989 No
BC vs. EDTA 0.00184 0.501 0.999 No
HM vs. EDTA 0.00184 0.501 0.997 No
EDTA vs. BC-EDTA 0.00140 0.468 0.994 No
BC-EDTA vs. EDDS 0.00139 0.464 0.984 No
BC-EDTA vs. BC-EDDS 0.00155 0.422 0.966 No
EDDS vs. BC-EDDS 0.000160 0.0436 0.999 No
BC vs. HM 3.469E-018 8.172E-016 1.000 No
Comparisons for factor: Treatment within Second sampling
Comparison Diff of Means t P P<0.05
EDTA vs. HM 0.0170 6.949 <0.001 Yes
BC-EDDS vs. HM 0.0136 5.562 <0.001 Yes
BC-EDTA vs. HM 0.0136 5.548 <0.001 Yes
EDTA vs. EDDS 0.0123 5.004 <0.001 Yes
BC vs. HM 0.00957 3.903 0.006 Yes
BC-EDDS vs. EDDS 0.00887 3.617 0.012 Yes
BC-EDTA vs. EDDS 0.00883 3.604 0.011 Yes
EDTA vs. BC 0.00747 3.046 0.040 Yes
BC vs. EDDS 0.00480 1.958 0.354 No
EDDS vs. HM 0.00477 1.945 0.320 No
BC-EDDS vs. BC 0.00407 1.659 0.437 No
BC-EDTA vs. BC 0.00403 1.645 0.377 No
EDTA vs. BC-EDTA 0.00343 1.401 0.434 No
EDTA vs. BC-EDDS 0.00340 1.387 0.322 No
BC-EDDS vs. BC-EDTA 0.0000333 0.0136 0.989 No
Comparisons for factor: Treatment within Third sampling
Comparison Diff of Means t P P<0.05
EDTA vs. EDDS 0.0339 13.844 <0.001 Yes
BC-EDTA vs. EDDS 0.0307 12.538 <0.001 Yes
EDTA vs. BC-EDDS 0.0299 12.212 <0.001 Yes
BC-EDTA vs. BC-EDDS 0.0267 10.906 <0.001 Yes
BC vs. EDDS 0.0262 10.702 <0.001 Yes
BC vs. BC-EDDS 0.0222 9.071 <0.001 Yes
EDTA vs. HM 0.0203 8.268 <0.001 Yes
BC-EDTA vs. HM 0.0171 6.963 <0.001 Yes
HM vs. EDDS 0.0137 5.576 <0.001 Yes
BC vs. HM 0.0126 5.127 <0.001 Yes
HM vs. BC-EDDS 0.00967 3.944 0.003 Yes
EDTA vs. BC 0.00770 3.141 0.016 Yes
BC-EDTA vs. BC 0.00450 1.836 0.215 No
BC-EDDS vs. EDDS 0.00400 1.632 0.216 No
EDTA vs. BC-EDTA 0.00320 1.306 0.203 No