1. Will the Animals Be Immunized?

F. IMMUNIZATION

Yes / No
1. Will the animals be immunized? / ☐ / ☐
2. Will the animals be immunized to produce antibodies? / ☐ / ☐
3. Are you using adjuvants? / ☐ / ☐

If you selected “NO” for all of the above, continue to section G.

a. Type of adjuvant
b. Dose of adjuvant
i. Initial injection
ii. Subsequent injections
c. Location of injection
d. Number of injection sites
e. Number of immunizations

4. a. Does your protocol comply with the HCCM Immunization Guidelines? Yes ☐ No ☐

b. If "NO", explain how it varies and justify the variations:

Variance and Justification

c. According to the IACUC Policy on the Use of Complete Freund’s Adjuvant:

·  The IACUC will consider justified applications for the use of CFA for primary injections.

·  CFA will not be allowed for boosters.

Justification for the use of CFA

5. a. Are you performing footpad injections? Yes ☐ No ☐

b. If "YES", you must justify the use of footpad injections rather than other methods of immunization and you must provide assurance that the animals will be housed on soft bedding and have easy access to food and water.

NOTE: Footpad injections are allowed in ONE FOOT PER ANIMAL and are permitted only if justification is deemed sufficient by the IACUC.

Justification for footpad injection:

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