UNIVERSITY OF WESTMINSTER

School of Biosciences

Control of Substances Hazardous to Health (COSHH)

***** APPENDIX 4 - CHEMICAL SAFETY *****

Sheet...... of......

Title of Experiment Room/Laboratory Site

1. / Sandwich ELISA Development with OPD / 5.08 / NCS

Brief description of work

Sandwich ELISA developed with OPD

Note that a COSHH assessment for class activity may not be of a sufficiently high level to cover technician preparation; double assessment usually required. Note also that it is RISK that is to be assessed, not HAZARD.

2.LIST OF SUBSTANCES USED OR CREATED (continue on a separate sheet if necessary)

NAME OF SUBSTANCE
(CODE) / PHYSICAL
DESCRIPTION / MAX.
QUANTITY
EXPOSED TO / DATA AVAILABLE
(YES/NO) / KNOWN HAZARDS
(See Note;
use numbers)
Sodium bicarbonate / Solid / G / Y / 5, (harmful)
7 (irritant)
Sodium hydrogen carbonate / Solid / G / Y / 5, (harmful)
7 (irritant)
Sodium chloride (salt) / Solid / G / Y / 5, (harmful)
7 (irritant)
Potassium chloride / Solid / G / Y / 5, (harmful)
7 (irritant)
di-sodium hydrogen phosphate / Solid / G / y / 5, (harmful)
7 (irritant)
Potassium dihydrogen phosphate / Solid / G / Y / 5, (harmful)
7 (irritant)
Sulphuric acid / Liquid / Ml / Y / 5 (harmful)
7 (irritant)
Citric acid / Solid / G / Y / 7 (irritant)
Hydrogen peroxide / Liquid / Ml / Y / 2 (oxidising)
6 (corrosive)
Orthophenylamine diamine (OPD) / Solid / Mg / Y / 4 (toxic)
5 (harmful)
7 (irritant)
8 (may cause cancer)

NOTE: 1) EXPLOSIVE 2) OXIDIZING 3) FLAMMABLE 4) TOXIC 5) HARMFUL 6) CORROSIVE

7) IRRITANT 8) CARCINOGEN 9) MUTAGEN 10) TERATOGEN 11) DUST 12) INHALATION

13) INGESTION 14) SKIN ABSORPTION 15) SKIN OR EYE 16) INJECTION 17) MAXIMUM EXPOSURE LIMIT

18) OCCUPATIONAL EXPOSURE STANDARD 19) RADIATION 20) OTHER (specify) Ref Note 17, 18: see EH40 (current edition).

3.PERSONNEL INVOLVED WITH SUBSTANCES (continue on a separate sheet if necessary)

NAME/CLASS / INITS / STATUS / INVOLVEMENT - DATE OF USE or
DAILY/WEEKLY/MONTHLY/OCCASIONALLY
Dwek / MV / Supervisor / Lecturer / Occassionally

4.OTHER GROUPS/PERSONS WHO MAY HAVE ACCESS TO THE SUBSTANCES

(e.g. students, cleaners, maintenance staff, contractors, visitors, storekeepers, etc.):

Stores

State control measures (to prevent inappropriate persons/groups having access to the substances):

Store in locked cabinet with other corrosive / toxic materials. Access to named personnel only. Cabinet should be well ventilated.

5.EMERGENCY PROCEDURES (as per Hazard Data Sheet).

if any of the substances or procedures identified above is likely to pose a special hazard in an emergency, then identify below action to be taken.

CODE / SPILLAGE/UNCONTROLLEDRELEASE / FIRE
Sodium bicarbonate / Wear respirator, chemical safety goggles, rubber boots and heavy rubber gloves. Sweep up, place in bag and hold for waste disposal Avoid raising dust and ventilate area and wash spill site after material pick-up is complete. / Use a water spray, carbon dioxide or dry chemical powder / foam. Emits toxic fumes under fire conditions.
Sodium hydrogen carbonate / Ditto / Ditto
Sodium chloride (salt) / Ditto / Ditto
Potassium chloride / Ditto / Ditto
di-sodium hydrogen phosphate / Ditto / Ditto
Potassium dihydrogen phosphate / Ditto / Ditto
Sulphuric acid / Wear respirator, chemical safety goggles, rubber boots and heavy rubber gloves. Absorb on sand and place in closed containers for disposal. Ventilate area and wash spill site after material pick-up is complete. / Ditto
Citric acid / Wear respirator, chemical safety goggles, rubber boots and heavy rubber gloves. Sweep up, place in bag and hold for waste disposal Avoid raising dust and ventilate area and wash spill site after material pick-up is complete. / Ditto
Hydrogen peroxide / Wear respirator, chemical safety goggles, rubber boots and heavy rubber gloves. Absorb on sand and place in closed containers for disposal. Ventilate area and wash spill site after material pick-up is complete. / Ditto
Orthophenylamine diamine (OPD) / Wear respirator, chemical safety goggles, rubber boots and heavy rubber gloves. Sweep up, place in bag and hold for waste disposal Avoid raising dust and ventilate area and wash spill site after material pick-up is complete. / Dissolve or mix with a combustible solvent and burn in a chemical incinerator equipped with an afterburner and scrubber.
If personnel are affected (fumes, contamination, etc.) treatment to be adopted:SPECIFY PER SUBSTANCE
(CODE AS PER HAZARD DATA SHEET)
sodium bicarbonate, sodium hydrogen carbonate, sodium chloride, potassium chloride, di sodium hydrogen phosphate, potassium dihydrogen phosphate
If swallowed, wash out mouth with water provided if person is conscious. Call a physician.
If inhaled remove to fresh air. If breathing becomes difficult call a physican. In case of contact immediately wash skin with soap and copious quantities of water. In case of contact with eyes, flush with copious quantities of water for at least 15 minutes. Assure adequate flushing by separating eyelids with fingers. Call a physician.
Sulphuric acid, citric acid, hydrogen peroxide
If swallowed, wash out mouth with water provided if person is conscious. Call a physician.
If inhaled remove to fresh air. If breathing becomes difficult call a physican. In case of contact immediately wash skin with soap and copious quantities of water. In case of contact with eyes, flush with copious quantities of water for at least 15 minutes. Assure adequate flushing by separating eyelids with fingers. Call a physician.
Orthophenylamine diamine
In case of an accident or if you feel unwell, seek medical advice immediately. In case of contact with eyes rinse immediately with plenty of water and seek medical advice.
(NB. Antidotes and special treatment may be obtained through...... )

6.CONTROL MEASURES TO BE ADOPTED (NB. Consider the following points, the appropriate Biosciences Safety Code plus any other necessary measures. SPECIFY PER SUBSTANCE AS NECESSARY FOR EACH OF THE ITEMS BELOW.)

Always use TFA in fume cupboard, wear gloves (see COSHH form attached). Always add acid to water in small volumes NOT water to acid.

1. Limiting exposure time of personnel - state maximum time

2. Partial enclosure with local exhaust ventilation

3. Local exhaust ventilation

4. Sufficient general ventilation

5. Personal protective equipment (goggles/gloves/clothing/mask/breathing apparatus/safety screen, etc.) SPECIFY

* 6. Safe disposal/storage of hazardous substances

* 7. Regular cleaning of the workplace

* 8. Adequate washing facilities* = MANDATORY

STORAGE - SAFETY CONSIDERATIONS
Sulphuric acid – toxic store cabinet
Citric acid – shelf of lab.
Hydrogen peroxide - fridge
Orthophenylamine diamine - fridge
Sodium and potassium salts – lab shelf / HANDLING PRECAUTIONS
Always wear gloves and goggles.
Use in fume hood.
Wear gloves and avoid breathing dust
Wear gloves and avoid breathing dust
DISPOSAL PROCEDURES DURING AND AT END OF EXPERIMENT
Dispose of plasticware after autoclaving via laboratory waste route. Dispose acid solutions via chemical disposal officer.

7.REVIEW AND MONITORING OF CONTROL MEASURES

(Required checks, and their frequency, on the adequacy and maintenance of the control measures during the course of the experiment)

(NB. Biosciences Safety Code in respect of health surveillance needs. This form is not adequate if health surveillance is required.)

8.OTHER RELEVANT INFORMATION (continue on a separate sheet if necessary)

(e.g. restriction of usage; transportation risks; labelling requirements; assessment review date, etc.)

9.I certify that, to the best of my knowledge, the above precautions will adequately control the risks from the hazardous substances listed. I have brought the assessment to the notice of the relevant technicians; academic staff; students; researchers (delete as necessary).

Name of Assessor:Miriam DwekSigned:

Status of Assessor:LecturerDate: