UNIT 5: DNA Replication Lab
X & CP Biology II, Mr. Doc Miller
North Central High School
Name: Type Your Name Here Period: Type Your Period Here Date: Type the Date Here
LAB: Transcription
Computer Based Lab: Complete the computer-based lab entitled “CBL 3 Transcription” (using “ActiveInspire” software.) Insert the “snapshot” in the space to the right, and then complete the analysis questions by typing your responses in the spaces provided (don’t forget your name, period and date above.) Before printing your final paper, delete blank spaces and make sure your work fits on TWO PAGES. Click on “File”, then “Print”, then click on “Copies & Pages” and select “Layout”. Change the “Pages per Sheet” to 2. Print (it should print both pages on one sheet).
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Analysis and Conclusion: (Complete sentences are required for #1, 3 & 6.)
1. How many nucleotides are responsible for coding one amino acid?
Type Your Answer Here
2. The following amino acid sequence represents a portion of the normal hemoglobin molecule.
PROLINE, GLUTAMIC ACID, GLUTAMIC ACID, LYSINE
Translate the sequence of amino acids in normal hemoglobin into:
a. DNA base codes with the sequence orders which coded for the mRNA
Type Your Answer Here
b. mRNA base codes needed to code the DNA instructions:
Type Your Answer Here
c. tRNA base codes needed to code the DNA message:
Type Your Answer Here
The following amino acid sequence represents a portion of the sickle cell hemoglobin molecule.
PROLINE, VALINE, GLUTAMIC ACID, LYSINE
Translate the sequence of amino acids in sickle cell hemoglobin into:
a. DNA base codes with the sequence orders which coded for the mRNA
Type Your Answer Here
b. mRNA base codes needed to code the DNA instructions:
Type Your Answer Here
c. tRNA base codes needed to code the DNA message:
Type Your Answer Here
3.Refer to question #2. Compare the DNA code for the normal hemoglobin to that of the DNA that makes the wrong hemoglobin molecule. What difference in the DNA must cause this difference in the DNA must cause this difference in the hemoglobin?
Type Your Answer Here
4.Using the codon wheel and what you know about complimentary base pairs, fill in the following chart.
DNA Code / mRNA code / tRNA code / Amino AcidAGT
GGGAAT
CTT
ATA
GCT
GTT
AAA
CAA
TTT
5.Write a 2-3-paragraph summary on protein synthesis. Use the follow words in your description (UNDERLINE THEM AS THEY APPEAR IN YOUR ANSWER): protein synthesis, DNA, mRNA, tRNA, ribosome.
*Delete blank spaces and make sure your work fits on ONE PAGE. Then print and turn in.* Grade: _____/20