UCSF Lab Standard Operating Procedure

Chemical Process, Name or Hazard Class:

Copper (II) Acetoarsenite

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Type of SOP: ☐ Process ☒Hazardous Chemical ☐ Hazardous Class

Purpose

Copper (II) Acetoarsenite is highly toxic.

Contains arsenic, a known carcinogen.

Very harmful if inhaled, swallowed or in contact with the skin or eyes. Can also be fatal if inhaled or ingested. Exposure can cause damage to the respiratory tract, kidneys, liver, and central nervous system.

Many synonyms exist including Paris green, Schweinfurt Green, Imperial Green, Vienna Green, Mitis Green, Veronese green.

Used as an agricultural pesticide and pigment.

Physical & Chemical Properties/Definition of Chemical Group

CAS#: 12002-03-8

Class: Toxic, carcinogenic

Molecular Formula: Cu(C2H3O2)2·3Cu(AsO2)2

Form (physical state): Powder

Color: Green powder

Boiling point: NA

Potential Hazards/Toxicity

Copper (II) Acetoarsenite is highly toxic and carcinogenic. Patients may rapidly become hypotensive with rapid heart rate secondary to pain, cardiac effects of arsenic or anxiety. Toxic delirium, seizures, peripheral neuropathy and encephalopathy can occur. Arsenic is not likely to be a significant risk to human reproduction at permissible occupational exposure limits.

Ingestion: Can be fatal. Symptoms include abdominal pain, vomiting, profuse bloody or watery (rice-water-like) diarrhea, pain in the extremities and muscles, weakness. Inhalation can cause acute respiratory failure and pulmonary edema. Adult respiratory distress syndrome (ards) has been reported. Avoid skin or eye contact.

Copper (II) Acetoarsenite has a threshold limit value of 0.01 mg/m3 TWA (as As, related to Arsenic, inorganic compounds)and 1 mg/m3 TWA (as Cu, dust and mist, related to Copper compounds)

Copper (II) Acetoarsenite has an oral toxicity of 22 mg/kg (LD50 Rat) and dermal 2400 mg/kg (LD50 Rat).

Engineering Controls

Handle using a chemical fume hood with good ventilation and electrically grounded lines and equipment.

Personal Protective Equipment (PPE)

Respirator Protection

If lab personnel would like to use respirator on a voluntary basis, they must be trained and fit-tested by EH&S. This is a regulatory requirement. (http://or.ucsf.edu/ehs/8193-DSY/version/default/part/4/data/)

Hand Protection

Gloves must be worn.

NOTE: Consult with your preferred glove manufacturer to ensure that the gloves you plan on using are compatible with Copper (II) Acetoarsenite.

Refer to glove selection chart from the links below:

http://www.ansellpro.com/download/Ansell_8thEditionChemicalResistanceGuide.pdf

OR

http://www.allsafetyproducts.biz/page/74172

OR

http://www.showabestglove.com/site/default.aspx

OR

http://www.mapaglove.com/

Eye Protection

ANSI approved properly fitting safety glasses or chemical splash goggles.

Skin and Body Protection

Flame resistant lab coats must be worn and be appropriately sized for the individual and buttoned to their full length. Laboratory coat sleeves must be of sufficient length to prevent skin exposure while wearing gloves. Full length pants and close-toed shoes must be worn at all times by all individuals that are occupying the laboratory area. The area of skin between the shoe and ankle should not be exposed.

Hygiene Measures

Wash thoroughly and immediately after handling. Remove contaminated clothing and wash before reuse.

First Aid Procedures

If inhaled

Move into the fresh air immediately and give oxygen. If not breathing give artificial respiration. Get medical attention immediately.

In case of skin contact

Immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes. Wash clothing before reuse. Thoroughly clean shoes before reuse. Get medical attention immediately.

In case of eye contact

Check for and remove any contact lenses. Rinse thoroughly with plenty of water for at least 15 minutes and consult a physician. Seek immediate medical attention and continue eye rinse during transport to hospital.

If swallowed

Do NOT induce vomiting unless directed by medical personnel. Never give anything by mouth to an unconscious person. Seek medical attention immediately.

Special Handling and Storage Requirements

To keep contamination to a minimum, all work with Copper (II) Acetoarsenite or arsenic chemicals should be done in a properly designated area with secondary containment and proper labeling. Wash hands thoroughly after handling. Minimize the generation and accumulation of dust. Avoid contact with eyes, skin, and clothing. Keep containers tightly closed. Store in a cool, dry and well-ventilated area away from incompatible substances.

Spill and Accident Procedure

Chemical Spill Dial 9-911 from campus phone or 415-476-1414 from cell phone or 415-206-8522 (SFGH only)

Spill – Assess the extent of danger. Assist contaminated or injured persons. Evacuate the spill area. Avoid breathing vapors. If possible, confine the spill to a small area using a spill kit or absorbent material. Keep others from entering contaminated area (e.g., use caution tape, barriers, etc.).

Small (<1 L) – If you have training, you may assist in the clean-up effort. Use appropriate personal protective equipment and clean-up material for chemical spilled. Double bag spill waste in clear plastic bags, label and take to the next chemical waste pick-up.

Large (>1 L) – Dial 9-911 from campus phone or 415-476-1414 from cell phone or 415-206-8522 (SFGH only) for assistance.

Chemical Spill on Body or Clothes – Remove clothing and rinse body thoroughly in emergency shower for at least 15 minutes. If discomfort persists, proceed to the Emergency Department. If no further discomfort is experienced, have the SDS ready and contact Poison Control Hotline at 1-800-222-1222 for further exposure information. Notify your direct supervisor and EH&S at 415-476-1300 during work hours, or 9-911 during non-working hours and weekends.

Chemical Splash Into Eyes – Immediately rinse eyeball and inner surface of eyelid with water for 15 minutes by forcibly holding the eye open. If discomfort persists, proceed to the Emergency Department. If no further discomfort is experienced, have the SDS ready and contact Poison Control Hotline at 1-800-222-1222 for further exposure information. Notify your direct supervisor and EH&S at 415-476-1300 during work hours, or 9-911 during non-working hours and weekends.

Medical Emergency Dial 9-911 (campus phone) or 476-6911 (cell phone)

Note: All serious injuries must be reported to EH&S at 415-476-1300 within 8 hours.

Non-Life Threatening Emergency– Go to Occupational Health Programs (OHP) Clinic, 415-885-7580, 2330 Post Street, Suite 460 Hours of Operation for Appointments: Monday - Friday 7:30 a.m. - 4:00 p.m. (except Holidays).

Note: All serious injuries must be reported to EH&S at 415-476-1300 within 8 hours.

Needle stick/puncture exposure (as applicable to chemical handling procedure) – Wash the affected area with antiseptic soap and warm water for 15 minutes. For mucous membrane exposure, flush the affected area for 15 minutes using an eyewash station. Page the needle stick nurse by dialing 415-353-7842 (STIC).

Decontamination/Waste Disposal Procedure

Clean contaminated surfaces with soap and water and paper towels. Dispose of the paper towels as hazardous waste.

Safety Data Sheet (SDS) Location

Online SDS can be accessed at http://or.ucsf.edu/ehs/7241-DSY/msds.html

Protocol/Procedure

Quantities covered by this SOP:

______(g , ml) to ______(g, ml)

Temperature range covered by this SOP:

__ °C – __ °C

General Overview and Purpose:

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Procedure:

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NOTE

Any deviation from this SOP requires approval from the Principal Investigator.

Copper (II) Acetoarsenite 2 Date: Click here to enter a date.

Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)