Facility Inspection of “Insert Facility Name and Fall/Spring, Year”

UC Davis Institutional Animal Care and Use Committee

Semi-Annual Facility Inspection

Name of Reviewed Unit:

This review covers the period from:

Date(s) of Review:

Name of Reviewer(s) and date(s) they attended:

Signatures:

Conducted with:

Instructions:

Indicate your response by making a check in the box provided. Make

comments in the margin on the back of page. Unless otherwise noted, correction of all identified “Minor Deficiencies” must occur within 30 days from the date of inspection. Alternate dates may be negotiated at the time of the inspection, but must be identified in writing. If “Significant Deficiencies” are noted, corrective actions are expected to be implemented immediately, with final correction dates determined by the UC Davis IACUC.

A=Acceptable

M=Minor

S=Significant

N/A=Not Applicable

Comments:

UC Davis Institutional Animal Care and Use Committee

Semi-Annual Facility Inspection

I.Training and Occupational Health

Personnel Qualifications and Training / A / M / S / n/a / Fol/up
1. Is the animal care staff adequately trained?
2. Is the staff training appropriately documented?
3. Is the research staff trained (ex. Facility Orientation for new users)?
24. Other

Occupational Health and Safety Program

Hazard Identification and Risk Assessment / A / M / S / n/a / Fol/up
25. Are personnel enrolled in the occupational health program?
26. Are inherent dangers identified and evaluated? Bites, chemical cleaning agents, allergens, zoonotic disease, etc.
27. Are procedures in place to identify and manage animal experiments that involve the use of hazardous agents such as: biohazards, chemicals, carcinogens, and volatile anesthetics, and is appropriate safety signage posted?
48. Other
Personal Hygiene, Protection / A / M / S / n/a / Fol/up
49. Is appropriate protective clothing and personal protective equipment provided to employees (e.g. uniforms, lab coats, goggles, hearing protection, etc)?
51. Proper enforcement of PPE use?
52. Are hand washing facilities available?
53. Is eating, drinking, using tobacco, or applying cosmetics is prohibited in the vivarium.
54. Is a breakroom provided?
55. Proper scavenging of anesthetic gas?
56. Is safety equipment properly maintained (e.g. eye wash, emergency shower)?
57. Are gas cylinders properly secured?
78. Other

II.Animal Environment, Housing, and Management

Physical Environment

General Physical Environment / A / M / S / n/a / Fol/up
79. Are protocols posted and current?
80. Are facility SOPs reviewed regularly?
81. Are appropriate signage/logs posted and maintained? (Daily check sheets, temp log.)
82. Is Fish and Game permit posted for detrimental species?
83. Is the facility in good repair (paint, floors, ceiling, etc.)
104. Other
Housing – Primary Enclosures / A / M / S / n/a / Fol/up
106. Are the enclosures in good repair (free of rust and sharp edges)?
107. If wire bottom cages are used for rodents, is it justified & approved by the IACUC?
129. Other
Housing – Sheltered and Outdoor / A / M / S / n/a / Fol/up
130. Does outdoor housing have adequate weather protection and ventilation?
131. Is housing sanitized/cleaned on a regular basis (no waste/moisture buildup)?
152. Other
Space Recommendations / A / M / S / n/a / Fol/up
153. Are animals housed in accordance with the Animal Welfare Regulations and the ILAR Guide and Ag Guide?
156. Has special consideration and careful review and justification been made for prolonged restraint?
177. Other
Temperature / A / M / S / n/a / Fol/up
178. Are temperatures maintained in accordance with the ILAR Guide, page 32?
201. Other
Ventilation / A / M / S / n/a / Fol/up
202. Is the HVAC system reviewed and maintained by a qualified mechanical engineer? (Recommended practice)
203. Do animal rooms meet the standard of 10-15 fresh-air changes per hour?
206. Is all of the animal-area air exhausted or recirculated only to animal areas so as to avoid contamination of people areas?
228. Other
Illumination / A / M / S / n/a / Fol/up
Have the following factors been considered with regards to lighting?
229. Length of photo period
230. Photo intensity, quality of light and distribution of light
231. Are light timers used to control photo periods?
232. Is timer performance checked periodically including after normal hours check?
253. Other
Noise Levels / A / M / S / n/a / Fol/up
255. Within your facility do you avoid noise above 85dB?
256. Are normal operations conducted so as to minimize noise that may affect animals?
277. Other

Behavioral Management

Structural and Social Environment, and Activity / A / M / S / n/a / Fol/up
278. Does the environment enhance animal well-being as appropriate to the species?
279. Are social animals given the opportunity to interact with other members of the species or humans, as appropriate?
280. Do cats have a raised resting surface?
281. Is there a Psychological Well-Being Plan for NHP’s?
282. Have all exemptions been evaluated and approved by the IACUC?
283. Do animals have the opportunity to exhibit species-typical activity patterns?
285. Are dogs given the opportunity to exercise, as applicable.
306. Other

Husbandry

Food General / A / M / S / n/a / Fol/up
307. Do all animals have ready access to appropriate food?
311. Is the feed of some animals restricted as appropriate? Rabbits, pigs, dogs, cats, NHP etc.
313. Has special consideration and careful review and justification been made for food regulation?
314. Is food regulation IACUC approved and monitored if IACUC required? (example:animal weight recorded).
335. Other
Food Storage / A / M / S / n/a / Fol/up
336. Is feed stored appropriately? (i.e. protected from vermin, off of the floor, stored<70F, conditions monitored on a regular basis.)
337. Are purified and chemically defined diets stored below 39F?
339. Are special autoclavable diets used when required and is the date of autoclaving recorded so that diets are rotated and used quickly?
340. Is the transfer of feed-storage containers between areas that pose different risks of contamination avoided?
341. Are storage containers cleaned regularly?
362. Other
Water / A / M / S / n/a / Fol/up
363. Do animals have access to potable drinking water?
364. Are watering devices checked daily?
366. Has special consideration and careful review and justification been made for water regulation?
367. Is water regulation IACUC approved? And monitored if IACUC required? (example: animal weight recorded).
388. Other
Bedding / A / M / S / n/a / Fol/up
389. Do you avoid softwood (pine is a softwood)bedding in those studies that may be affected by the aromatic hydrocarbons they contain?
392. Are bedding materials stored appropriately
393. If autoclaved, is bedding dried before use or storage?
414. Other
Sanitation General / A / M / S / n/a / Fol/up
415. Are cleaning implements dedicated to areas of similar contamination risk?
416. Are worn cleaning implements replaced as required?
418. Are animal rooms and procedure areas properly cleaned?
439. Other
Sanitation Caging / A / M / S / n/a / Fol/up
441. Are pens and runs, not using bedding, cleaned and sanitized appropriately?
442. Are animals kept dry during cage cleaning as appropriate?
443. Are cages and accessories such as tops sanitized appropriately?
447. Is the effectiveness of the method of sanitization appropriately monitored (i.e. verified by use of systems such as RODAC plates)?
450. Are autoclaves monitored by systems such as kilits®?
471. Other
Cagewash Area / A / M / S / n/a / Fol/up
Does the cage washer/cleaning area provide the following:
473. Appropriate animal waste disposal?
474. Ease of access (including door size) facilitating use?
477. Traffic flow clean to dirty with no contamination of clean equipment by dirty equipment?
479. Are cage wash temperature monitored and are records available?
480. Appropriate clean cage storage?
501. Other
Waste Disposal / A / M / S / n/a / Fol/up
502. Is conventional, biological, and hazardous waste removed and disposed of regularly and safely?
504. Are receptacles leak proof and equipped with tight-fitting lids?
505. Are containers cleaned often and regularly?
508. Do employees avoid re-capping needles?
530. Other
Pest Control / A / M / S / n/a / Fol/up
531. Is anappropriate pest control program in place?
532. Have steps been taken to eliminate harborage (places that insects inhabit, such as cracks, dark and dirty spaces, and areas of trash and debris) and prevent entry?
533. Are the use of chemical pesticides limited and controlled?
535. If traps are used, are they checked frequently to assure humane euthanitization after capture?
556. Other
Emergency, Weekend, and Holiday Care / A / M / S / n/a / Fol/up
557. Are animals cared for by qualified personnel each and every day?
558. Is emergency veterinary care available each and every day?
559. Do police and fire agencies have access to telephone numbers of key personnel?
560. Is there a disaster plan?
561. Are key vivaria personnel included as official responders to emergencies that involve animals?
582. Other

Population Management

Identification and Records / A / M / S / n/a / Fol/up
583. Are all animals identified appropriately (Source, pertinent dates, protocol #, sex, etc.)?
584. Are appropriate medical records maintained and readily available as required?
585. If micro chipped, is a working reader readily available?
586. Are appropriate acquisition/disposition records maintained for 3 years?
606. Other

III.Veterinary Medical Care

General Requirements for Adequate Veterinary Care: / A / M / S / n/a / Fol/up
607. Is there a program for preventative medicine?
608. Is there a program for the surveillance, diagnosis, treatment, and control of natural diseases or conditions (including sub-clinical diseases)?
609. Is there a program to manage diseases or conditions induced by experimental conditions?
610. Is there a working program for reporting sick and dead animals to the veterinarian? (Communication with the veterinarian should be timely and regular.)
611. Are antibiotics, analgesics and anesthetics used according to the IACUC approved protocol?
612. Are expired drugs used?
613. Medical materials (sutures, Betadine, etc) are not used beyond their expiration date.
632. Other
Animal Procurement and Transportation / A / M / S / n/a / Fol/up
635. Is transportation consistent with USDA regulations and humane practices?
636. Are transportation vehicles clean and does transportation minimize the spread of disease and stress of the animals?
638. Is ordering and receiving of animals coordinated with the appropriate animal care personnel to assure that appropriate facilities are available for housing?
639. Are campus policies on transportation and procurement followed?
660. Other

Preventative Medicine

Quarantine, Stabilization, and Separation / A / M / S / n/a / Fol/up
661. Does the veterinarian or delegated person evaluate the health and pathogen status of newly received animals?
662. Is there an appropriate quarantine program? (For animals that are not from a known specific-pathogen-free facility) (For animals with known pathogens: is the investigator notified, breeding avoided, plan to eliminate, avoid taking to labs or other vivaria?)
663. Are steps taken to separate species with a different microbiological profile?
664. Are animals given a period of stabilization before being used?
665. Are animals separated by species?
686. Other
Surgery / A / M / S / n/a / Fol/up
688. Is there a program for the continuing and thorough assessment of surgical outcomes? Is corrective action taken as appropriate?
689. Are anesthetic machines maintained appropriately?
For survival surgery, are the following practices followed except as exempted:
691. Routine preparation of patient?
692. Routine preparation of surgeon?
694. Use of sterile gloves, gowns, masks, etc, as appropriate?
695. Sterilization of instruments and implants, etc.? (Not alcohol)
696. Aseptic operative techniques?
697. Is the effectiveness of sterilization monitored by indicators or other methods?
698. Is non-rodent survival surgery conducted in an authorized location dedicated to the use and are the appropriate number of dedicated rooms available for different aspects of surgery?
699. Is the surgical area kept clean or thoroughly cleaned prior to surgical use?
700. Is a record of surgery and postoperative care maintained? Non-rodent surgery requires a more detailed record.
701. If neuromuscular blocking (NMB) agents are used are they specifically justified by the investigator and approved by the IACUC?
702. Are appropriate amounts of anesthetics first defined on procedures that do not use NMB agents?
723. Other
Euthanasia / A / M / S / n/a / Fol/up
724. Are methods consistent with AVMA recommendations and appropriate to the species?
726.Are individuals trained in the method of euthanasia to be used?
747. Other
Humane Endpoints / A / M / S / n/a / Fol/up
748. Is timely care or euthanasia provided to diseased or injured animals, or, if the scientific protocol causes a disease or condition, does the protocol have specific criteria for intervention by euthanasia before the condition leads to death?
770. Other

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Rev 8/23/07