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Development of SSR markers from Musa balbisiana for genetic diversity analysis among Thai bananas
Tiwa Rotchanapreeda, Sirapope Wongniam, Sasivimon Chomchalow Swangpol,
Piyarat Parinyapong Chareonsap, Narongsak Sukkaewmanee, Jamorn Somana†
Plant Systematics and Evolution
† Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok 10400, Thailand
Corresponding author e-mail: ; tel: +662 2015468; fax: +662 3547174
Online Resource 5 SSR loci information and position on designated chromosomes of BB genome: Musa balbisianaaccession ‘Pisung Klutuk Wulung’
Banana SSR markers – Primers Set 1
FAM / BB_GT-34.1
F: TGCCCTTCAATTCATGTCAA 5’to3’
R: TGGTATGCAGCAAGCAAAAG 5’to3’
SSR-enriched library clone sequence
Accession KM190147
>BB_GT-34.1
TTGGGTGGTGCGTGACACATCTGCCGCATAAGGATGATGATGCGCCATTACCCATACTTACCGTCCACACAGCTCCCTTGTTGGGCGAAGCTCGCATCTAAATTACATAATATATGGCGCTATTCGTGCCACGTTGCCCTGCTCTGCTTACACAGGAGGGACCACGCAGCCATAGTTGCTATTTCCATTTGGAAATTCAACCCCTCTTCCTGTGCTTCTCTTACAGCTATGTATGTATGTGCACACTGCTCTCTTTCTCTCTCTCAATCTGGTCTTTTTCTGTTCTCTCTCTGTGACTGATAAGCTGCTGCTATATATAAAAAGAGGGAGGGAGAGAGTAACCGCACCTCCACACCTCTTCCCAACTACTGACTGAACCCCATACCTTACCCTTCCCATCTCTTTTCTCTCAACCCACCATTGATGCCCTTCAATTCATGTCAACTACTCTTTTCTGAAGAGAGAGAGAAATTCCACCGCCATCACTTACTTCCCCTATTTGGGAAACACAAATACATATCTGAAGACACAAGCTGCAGCGGCGTGTTTCTGTTTGTGCTTCTTTTGCTTGCTGCATACCAAGTTATCGAGCCTAGTGAGAGGACAGAGGGAGAAACAATTGCATCATCCCTCACCTTCGCGACCGTCATAATTGGCCGCCAAAAAAGGATGGTGGGAGTCTATGGCGGTCGTGGTGATCAGGGGGTACAACCCGTAACGGGACCCGGGGGGAGCGGCTGTGGGAAGGGGGGTACAACGGCGCTGCCATGTCCGCCACTCCCCTTCCTTCCTCAGGGCCGCCGAATTCCTTCCCTTTTTCCTTTCCTTGGGTCCATTCTTTCCGAATAATCCCGGTTCCGGAGGGAACGCACTTGGACATCCTTTTGGGATCTTATTTCCCCCAGAAAATTGCGTATGCAAAAA
Musa balbisiana ‘Pisung Klutuk Wulung’ sequence at Banana Genome Hub
>chrUn_random:138564678..138565615
TCTTCGCATACGCATACTTACTGAGGAGAATAAGATCACAAAAGGATGTCCAAAGTGCGTTCACTCAGAAACAGGAGATGATCGGAAAGAATGGAACACAAAAAGAAAAAGAAAAAAGAGAAGGAATACGGACGAGCATGAGGAAGGAAGGGGAGTGGCGGACATGGCAGCGCCGCTGTACCCCACCTCCCACAGCCGCTCCCCCCGGGTCCCGCTACGCGTCGTACCCCCTGATCACCACGACCGCCATAGACTCCCACCATCCTTATATGGCGGCCAATTATGACGGTCGCGAAGGTGAGGGATGATGCAATTGTTTCTCCCTCTGTCCTCTCACTAGGCTCGATAACTTGGTATGCAGCAAGCAAAAGAAGAACAAACAGAAACACGCCGCTGCAGCTTGTGTCTTCACATATGGATGTGTCTTTCCCAAGTAGGGGAAGTGATGGCAGTGGAACTTCTCTCTTTCTTCAGAAAGGAGTAGTTAGCATGAATTGAAGGGAATCAATGGTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTTCAGTCAGTAGTTGGGAAGAGGTGTGGAGGTGCGGTTACTCTCTCCCTCCCTCTTTTTATATATAGCAGCAGCTTATCAGTCACAGAGAGAGAACAGAAAAAGACCAGATTGAGAGAGAGAAAGAGAGCAGTGTGCACATACATACATAGCTGTAAGAGAAGCACAGGAAGAGGGGTTGAATTTCCAAATGGAAATAGCAACTATGGCTGCGTGGTCCCTCTGCTTACTTCCTGTGTAAGCAGAGCAGGGCATCGTGGCACGAATAGCGCCATATATTATGTAATTTAGATGCGAGCTTCGCCCAACAAGGGAGCTGTGTGGACGGTAAGTATGGGTAATGGCGCATCATCATCCTTATGCGGCAGATGTGTCACGCACCACCCAA
CLUSTAL 2.1 multiple sequence alignment
Note: Sequence of BB_GT-34.1 clone is reverse complemented
BB_GT-34.1 TTTTTGCATACGCA-ATTTTCTGGGGGAAATAAGATCCCAAAAGGATGTCCAA-GTGCGT 58
chrUn_random TCTTCGCATACGCATACTTACTGAGGAGAATAAGATCACAAAAGGATGTCCAAAGTGCGT 60
* ** ********* * ** *** ** ********* *************** ******
BB_GT-34.1 TCCCTCCGGAACCGGGATTATTCGGAAAGAATGGACC-CAAGGA----AAGGAAAAAGGG 113
chrUn_random TCACTCAGAAACAGGAGATGATCGGAAAGAATGGAACACAAAAAGAAAAAGAAAAAAGAG 120
** *** * *** ** * ************** * *** * *** ****** *
BB_GT-34.1 AAGGAATTCGG-CGGCCCTGAGGAAGGAAGGGGAGTGGCGGACATGGCAGCGCCGTTGTA 172
chrUn_random AAGGAATACGGACGAGCATGAGGAAGGAAGGGGAGTGGCGGACATGGCAGCGCCGCTGTA 180
******* *** ** * ************************************* ****
BB_GT-34.1 CCCCCCTTCCCACAGCCGCTCCCCCCGGGTCCCGTTACGGGTTGTACCCCCTGATCACCA 232
chrUn_random CCCCACCTCCCACAGCCGCTCCCCCCGGGTCCCGCTACGCGTCGTACCCCCTGATCACCA 240
**** * *************************** **** ** *****************
BB_GT-34.1 CGACCGCCATAGACTCCCACCATCCTTTTTTGGCGGCCAATTATGACGGTCGCGAAGGTG 292
chrUn_random CGACCGCCATAGACTCCCACCATCCTTATATGGCGGCCAATTATGACGGTCGCGAAGGTG 300
*************************** * ******************************
BB_GT-34.1 AGGGATGATGCAATTGTTTCTCCCTCTGTCCTCTCACTAGGCTCGATAACTTGGTATGCA 352
chrUn_random AGGGATGATGCAATTGTTTCTCCCTCTGTCCTCTCACTAGGCTCGATAACTTGGTATGCA 360
************************************************************
BB_GT-34.1 GCAAGCAAAAGAAGCACAAACAGAAACACGCCGCTGCAGCTTGTGTCTTCAGATATGTAT 412
chrUn_random GCAAGCAAAAGAAGAACAAACAGAAACACGCCGCTGCAGCTTGTGTCTTCACATATGGAT 420
************** ************************************ ***** **
BB_GT-34.1 TTGTGTTTCCCAAATAGGGGAAGTAAGTGATGGCGGTGGAATTTCTCTCTCTCTTCAGAA 472
chrUn_random GTGTCTTTCCCAAGTAGGGGAAGT----GATGGCAGTGGAACTTCTCTCTTTCTTCAGAA 476
*** ******** ********** ****** ****** ******** *********
BB_GT-34.1 AAGAGTAGTTGACATGAATTGAAGGGCATCAATGGTGGGTTGAGAGAAAAGAGATGGGAA 532
chrUn_random AGGAGTAGTTAGCATGAATTGAAGGGAATCAATGGTGGNNNNNNNNNNNNNNNNNNNNNN 536
* ******** ************** ***********
BB_GT-34.1 GGGTAAGGTATGGGGTTCAGTCAGTAGTTGGGAAGAGGTGTGGAGGTGCGGTTACTCTCT 592
chrUn_random NNNNNNNNNNNNNGGTTCAGTCAGTAGTTGGGAAGAGGTGTGGAGGTGCGGTTACTCTCT 596
***********************************************
BB_GT-34.1 CCCTCCCTCTTTTTATATATAGCAGCAGCTTATCAGTCACAGAGAGAGAACAGAAAAAGA 652
chrUn_random CCCTCCCTCTTTTTATATATAGCAGCAGCTTATCAGTCACAGAGAGAGAACAGAAAAAGA 656
************************************************************
BB_GT-34.1 CCAGATTGAGAGAGAGAAAGAGAGCAGTGTGCACATACATACATAGCTGTAAGAGAAGCA 712
chrUn_random CCAGATTGAGAGAGAGAAAGAGAGCAGTGTGCACATACATACATAGCTGTAAGAGAAGCA 716
************************************************************
BB_GT-34.1 CAGGAAGAGGGGTTGAATTTCCAAATGGAAATAGCAACTATGGCTGCGTGGTCCCTC--- 769
chrUn_random CAGGAAGAGGGGTTGAATTTCCAAATGGAAATAGCAACTATGGCTGCGTGGTCCCTCTGC 776
*********************************************************
BB_GT-34.1 ------CTGTGTAAGCAGAGCAGGGCAACGTGGCACGAATAGCGCCATATATTATGTAA 822
chrUn_random TTACTTCCTGTGTAAGCAGAGCAGGGCATCGTGGCACGAATAGCGCCATATATTATGTAA 836
********************* *******************************
BB_GT-34.1 TTTAGATGCGAGCTTCGCCCAACAAGGGAGCTGTGTGGACGGTAAGTATGGGTAATGGCG 882
chrUn_random TTTAGATGCGAGCTTCGCCCAACAAGGGAGCTGTGTGGACGGTAAGTATGGGTAATGGCG 896
************************************************************
BB_GT-34.1 CATCATCATCCTTATGCGGCAGATGTGTCACGCACCACCCAA 924
chrUn_random CATCATCATCCTTATGCGGCAGATGTGTCACGCACCACCCAA 938
******************************************
Banana SSR markers – Primers Set 1
HEX / BB_GAA-7
F: GAATGTGAGCCCTCTTCTCG 5’to3’
R: ACTGGACCCGTTTCAGTGAC 5’to3’
SSR-enriched library clone sequence
Accession KM190148
>BB_GAA-7
AAAAAATCCTCAAATAGAAATTAGGGCTTGCGAATGTGAGCCCTCTTCTCGCGTTCAACACTGTTGCCATCTCTGCTGCCGCTACTTCTCCTCCTCTTCTTTCTTCTTCCTCCTCTTCCACCACTTCTTCTTCCTTCTTCTTCCTACTCCACCGCTTCTTCTTCTTCCTTCTTCCTCCTTGTTCCTCCACCACCCATTCCTCTTTTCCCTCTTTTTCTTCGTATCCAAAACACCAGTACATACAAGTCACTGAAACGGGTCCAGTACCCAAAACAACAATCCTTGAAAAAAACAATTAGTTTTCTTGCTAAAACAAGGCAACCCAATGCATAAAGTTCCTCCATATAGGGTCAAAAGAGAATCAATATATACAGTC
Musa balbisiana ‘Pisung Klutuk Wulung’ sequence at Banana Genome Hub
>chr10:12071990..12072365
GACTGTATATATTGATTCTCTTTTGACCCTATATGGAGGAACTTTATGCATTGGGTTGCCTTGTTTTAGCAAGAAAGCTAATTGTTTTTTTCAAGGATTGTTGTTTTGGGTACTGGACCCGTTTCAGTGACTTGTATGTACTGGTGTTTTGGATACGAAGAAAAAGAGGGAAAAGAGGAATGGGTGGTGGAGGAACAAGGAGGAAGAAGGAAGAAGAAGAAGCGGTGGAGTAGGAAGAAGAAGGAAGAAGAAGTGGTGGAAGAGGAGGAAGAAGAAAGAAGAGGAGGAGAAGTAGCGGCAGCAGAGATGGCAACAGTGTTGAACGCGAGAAGAGGGCTCACATTCGCAAGCCCTAATTTCTATTTGAGGATTTTTT
CLUSTAL 2.1 multiple sequence alignment
Note: Sequence of BB_GAA-7 clone is reverse complemented
BB_GAA-7 GACTGTATATATTGATTCTCTTTTGACCCTATATGGAGGAACTTTATGCATTGGGTTGCC 60
chr10 GACTGTATATATTGATTCTCTTTTGACCCTATATGGAGGAACTTTATGCATTGGGTTGCC 60
************************************************************
BB_GAA-7 TTGTTTTAGCAAGAAAACTAATTGTTTTTTTCAAGGATTGTTGTTTTGGGTACTGGACCC 120
chr10 TTGTTTTAGCAAGAAAGCTAATTGTTTTTTTCAAGGATTGTTGTTTTGGGTACTGGACCC 120
**************** *******************************************
BB_GAA-7 GTTTCAGTGACTTGTATGTACTGGTGTTTTGGATACGAAGAAAAAGAGGGAAAAGAGGAA 180
chr10 GTTTCAGTGACTTGTATGTACTGGTGTTTTGGATACGAAGAAAAAGAGGGAAAAGAGGAA 180
************************************************************
BB_GAA-7 TGGGTGGTGGAGGAACAAGGAGGAAGAAGGAAGAAGAAGAAGCGGTGGAGTAGGAAGAAG 240
chr10 TGGGTGGTGGAGGAACAAGGAGGAAGAAGGAAGAAGAAGAAGCGGTGGAGTAGGAAGAAG 240
************************************************************
BB_GAA-7 AAGGAAGAAGAAGTGGTGGAAGAGGAGGAAGAAGAAAGAAGAGGAGGAGAAGTAGCGGCA 300
chr10 AAGGAAGAAGAAGTGGTGGAAGAGGAGGAAGAAGAAAGAAGAGGAGGAGAAGTAGCGGCA 300
************************************************************
BB_GAA-7 GCAGAGATGGCAACAGTGTTGAACGCGAGAAGAGGGCTCACATTCGCAAGCCCTAATTTC 360
chr10 GCAGAGATGGCAACAGTGTTGAACGCGAGAAGAGGGCTCACATTCGCAAGCCCTAATTTC 360
************************************************************
BB_GAA-7 TATTTGAGGATTTTTT 376
chr10 TATTTGAGGATTTTTT 376
****************
Banana SSR markers – Primers Set 1
NED / BB_CT-7
F: ACGCAACGAGACACACAAAC 5’to3’
R: GAACGAGAAACTGCCTTTGC 5’to3’
SSR-enriched library clone sequence
Accession KM190149
>BB_CT-7
CCGAACGCAACGAGACACACAAACACAAAATTTGGCCAAAAAGTCCCATAATAATGCGCCAGAAAGAGAGATGCACTTACCGACGACATGATCGAGGAACAGAGAGGAAGAGAGAGAGTATATGGTGTTGAAAAGAAAGAGAGAGAAAGAGAGCGAGAGAGAGGGCGAGAGCAAGTCTCGGGGGAAGAAGACGCAGCAAGCAAAGGCAGTTTCTCGTTCTAAATATAGCAATCCCCCATCGGAAATTGTCTGATAATCGTTTGAATCCTTATTTCTTCATTTCCTTCGAAGAAAAGGTCGAATA
Musa balbisiana ‘Pisung Klutuk Wulung’ sequence at Banana Genome Hub
>chr11:15610191..15610494
CCGAACGCAACGAGACACACAAACACAAAATTTGGCCAAAAAGTCCCATAATAATGCGCCAGAAAGAGAGATGCACTTACCGACGACATGATCGAGGAACAGAGAGGAAGAGAGAGAGTATATGGTGTTGAAAAGAAAGAGAGAGAAAGAGAGCGAGAGAGAGGGCGAGAGCAAGTCTCGGGGGAAGAAGACGCAGCAAGCAAAGGCAGTTTCTCGTTCTAAATATAGCAATCCCCCATCGGAAATTGTCTGATAACCGTTTGAATCCTTATTTCTTCATTTCCTTCGAAGAAAAGGTCGAATA
CLUSTAL 2.1 multiple sequence alignment
BB_CT-7 CCGAACGCAACGAGACACACAAACACAAAATTTGGCCAAAAAGTCCCATAATAATGCGCC 60
chr11 CCGAACGCAACGAGACACACAAACACAAAATTTGGCCAAAAAGTCCCATAATAATGCGCC 60
************************************************************
BB_CT-7 AGAAAGAGAGATGCACTTACCGACGACATGATCGAGGAACAGAGAGGAAGAGAGAGAGTA 120
chr11 AGAAAGAGAGATGCACTTACCGACGACATGATCGAGGAACAGAGAGGAAGAGAGAGAGTA 120
************************************************************
BB_CT-7 TATGGTGTTGAAAAGAAAGAGAGAGAAAGAGAGCGAGAGAGAGGGCGAGAGCAAGTCTCG 180
chr11 TATGGTGTTGAAAAGAAAGAGAGAGAAAGAGAGCGAGAGAGAGGGCGAGAGCAAGTCTCG 180
************************************************************
BB_CT-7 GGGGAAGAAGACGCAGCAAGCAAAGGCAGTTTCTCGTTCTAAATATAGCAATCCCCCATC 240
chr11 GGGGAAGAAGACGCAGCAAGCAAAGGCAGTTTCTCGTTCTAAATATAGCAATCCCCCATC 240
************************************************************
BB_CT-7 GGAAATTGTCTGATAATCGTTTGAATCCTTATTTCTTCATTTCCTTCGAAGAAAAGGTCG 300
chr11 GGAAATTGTCTGATAACCGTTTGAATCCTTATTTCTTCATTTCCTTCGAAGAAAAGGTCG 300
**************** *******************************************
BB_CT-7 AATA 304
chr11 AATA 304
****
Banana SSR markers – Primers Set 1
PET / BB_CT-18
F: CACACCAACCTGTCTGCATC 5’to3’
R: CTGAGCAGTTCCCCACTAGC 5’to3’
SSR-enriched library clone sequence
Accession KM190150
>BB_CT-18
CCGAGAGAGGTGCCGTCCAACCCCTCCCAGTTGACACTCGGGTCCCTCTCTTCGTCCAACGATAGCGAGAGCTAGAGGGAGAAGAAGAAGAAGAGATAAAGAGGTGGGGAAAGGGAAAAAGTTGTGTCATCATCACATCACACCAACCTGTCTGCATCACAGAGAGAGAGAGAGGGAGAGAGAGAGAGAGAGAGAGTTGCTGGGATTCGAAGAAAACGTAGCAGAAGAAGATGATGGAGAAAAGCTTTACATGGCTGCTTCCCCTTGGCAGCATATATGCATCCCAACCATCTCCGAGTCCAAAGCTAGTGGGGAACTGCTCAGATCCAAGCAAAAGATGGATGCCGTGTGGGGCTGTGTGCATGCAGATAGCATGGTTTTTGGATTTGATGTGCACTACACCGAAACAGTAAGCTATATTTTCC
Musa balbisiana ‘Pisung Klutuk Wulung’ sequence at Banana Genome Hub
>chr10:20214030..20214432
GGAAAATATAGCTTACTGTTTCGGTGTAGTGCACATCAAATCCAAAAACCATGCTATCTGCATGCACACAGCCCCACACGGCATCCATCTTTTGCTTGGATCTGAGCAGTTCCCCACTAGCTTTGGACTCGGAGATGGTTGGGATGCATATATGCTGCCAAGGGGAAGCAGCCATGTAAAGCTTTTCTCCATCATCTTCTTCTGCTGCGTTTTCTTCGAATCCCAGCAACTCTCTCTCTCTCTCTCTCTCTCTCTCTGATGCAGACAGGTTGGTGTGATGTGATGATGACACAACTTTTTCCCTTTCCCCACCTCTTTATCTCTTCTTCTTCTTCTCGCTATCGTTGGACGAAGAGAGGGACCCGAGTGTCAACTGGGAGGGGTTGGACGGCACCTCTCTCGG
CLUSTAL 2.1 multiple sequence alignment
Note: Sequence of BB_CT-18 clone is reverse complemented
BB_CT-18 GGAAAATATAGCTTACTGTTTCGGTGTAGTGCACATCAAATCCAAAAACCATGCTATCTG 60
chr10 GGAAAATATAGCTTACTGTTTCGGTGTAGTGCACATCAAATCCAAAAACCATGCTATCTG 60
************************************************************
BB_CT-18 CATGCACACAGCCCCACACGGCATCCATCTTTTGCTTGGATCTGAGCAGTTCCCCACTAG 120
chr10 CATGCACACAGCCCCACACGGCATCCATCTTTTGCTTGGATCTGAGCAGTTCCCCACTAG 120
************************************************************
BB_CT-18 CTTTGGACTCGGAGATGGTTGGGATGCATATATGCTGCCAAGGGGAAGCAGCCATGTAAA 180
chr10 CTTTGGACTCGGAGATGGTTGGGATGCATATATGCTGCCAAGGGGAAGCAGCCATGTAAA 180
************************************************************
BB_CT-18 GCTTTTCTCCATCATCTTCTTCTGCTACGTTTTCTTCGAATCCCAGCAACTCTCTCTCTC 240
chr10 GCTTTTCTCCATCATCTTCTTCTGCTGCGTTTTCTTCGAATCCCAGCAACTCTCTCTCTC 240
************************** *********************************
BB_CT-18 TCTCTCTCTCCCTCTCTCTCTCTCTGTGATGCAGACAGGTTGGTGTGATGTGATGATGAC 300
chr10 TCTCTCTCTCTCTCTCT------GATGCAGACAGGTTGGTGTGATGTGATGATGAC 290
********** ****** *********************************
BB_CT-18 ACAACTTTTTCCCTTTCCCCACCTCTTTATCTCTTCTTCTTCTTCTCCCTCTAGCTCTCG 360
chr10 ACAACTTTTTCCCTTTCCCCACCTCTTTATCTCTTCTTCTTCTTC------TCG 338
********************************************* ***
BB_CT-18 CTATCGTTGGACGAAGAGAGGGACCCGAGTGTCAACTGGGAGGGGTTGGACGGCACCTCT 420
chr10 CTATCGTTGGACGAAGAGAGGGACCCGAGTGTCAACTGGGAGGGGTTGGACGGCACCTCT 398
************************************************************
BB_CT-18 CTCGG 425
chr10 CTCGG 403
*****
Banana SSR markers – Primers Set 2
FAM / BB_CT-40
F: GATAGAGGTGCCCACTCCAA5’to3’
R: ATGCCTTCTGCCTCGTCTT 5’to3’
SSR-enriched library clone sequence
Accession KM190151
>BB_CT-40
CCCAAGAAGAGAAGGAGCAGCATGGTATTGTGTGGAAGAAGAAAAAGGCTGCCTTGATATCCCATCGTGAACCCACCTTTCCTTCCATGATGAGATAGAGGTGCCCACTCCAACAGAGCCACCTTCAGATCAAACAAGGCAACCACAGCAGCTCAGCAAGACTTACCTTCTTCGCCAAAAAGAAATCAGCATCTTTTTACTCAGAGTGTGTGTGTGTGTGTGTGTGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGATGGCCCTCTTGGTAAGTGAAGATACTGACCTTGGGAGAGAGAGAGAGAGAGAGGGAAGACGAGGCAGAAGGCATTGGCATCCACATGGTGTGGCTCCTTCGA
Musa balbisiana ‘Pisung Klutuk Wulung’ sequence at Banana Genome Hub
>chr3:8629753..8630094
CCCAAGAAGAAAAGGAGCAGCATGGTATTGTGTGGAAGAAGAAAAAGGCTGCCTTGATATCCCATCATGAACCCACCTTTCCTTCCATGATGAGATAGAGGTGCCCACTCCAACAGAGCCACCTTCAGATCAAACAAGGCACCCACAGCAACTCAGCAAGACTTACCTTCTTCGCCAAAAAGAAATCAGCATCTTTTTACTCAGGAGTGTGTGTGTGTGTGTGTGTGTGTGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGTCGGAGAGAGAGAGAGAGAGAGAGGGAAGAGGAAGTAGTAGGCATTGGCATCCACATGGTGTGGCTCCTTCGA
CLUSTAL 2.1 multiple sequence alignment
BB_CT-40 CCCAAGAAGAGAAGGAGCAGCATGGTATTGTGTGGAAGAAGAAAAAGGCTGCCTTGATAT 60
chr3 CCCAAGAAGAAAAGGAGCAGCATGGTATTGTGTGGAAGAAGAAAAAGGCTGCCTTGATAT 60
********** *************************************************
BB_CT-40 CCCATCGTGAACCCACCTTTCCTTCCATGATGAGATAGAGGTGCCCACTCCAACAGAGCC 120
chr3 CCCATCATGAACCCACCTTTCCTTCCATGATGAGATAGAGGTGCCCACTCCAACAGAGCC 120
****** *****************************************************
BB_CT-40 ACCTTCAGATCAAACAAGGCAACCACAGCAGCTCAGCAAGACTTACCTTCTTCGCCAAAA 180
chr3 ACCTTCAGATCAAACAAGGCACCCACAGCAACTCAGCAAGACTTACCTTCTTCGCCAAAA 180
********************* ******** *****************************
BB_CT-40 AGAAATCAGCATCTTTTTACTCAG-AGTGTGTGTGTGTGTGTGTGTGAGAGAGAGAGAGA 239
chr3 AGAAATCAGCATCTTTTTACTCAGGAGTGTGTGTGTGTGTGTGTGTGTGTGAGAGAGAGA 240
************************ ********************** * **********
BB_CT-40 GAGAGAGAGAGAGAGAGAGATGGCCCTCTTGGTAAGTGAAGATACTGACCTTGGGAGAGA 299
chr3 GAGAGAGAGAGAGAGAGAGA------GAGAGAGAGTCGGAGAGAGA 280
******************** ** * ** * ******
BB_CT-40 GAGAGAGAGAGAGGGAAGACGAGGCAGAAGGCATTGGCATCCACATGGTGTGGCTCCTTC 359
chr3 GAGAGAGAGAGAGGGAAGAGGAAGTAGTAGGCATTGGCATCCACATGGTGTGGCTCCTTC 340
******************* ** * ** ********************************
BB_CT-40 GA 361
chr3 GA 342
**
Banana SSR markers – Primers Set 2
HEX / BB_CT-19
F: AGGAATTGCTAGTGGGCAGA 5’to3’
R: ACCATTTTGACCACCGTCTC 5’to3’
SSR-enriched library clone sequence
Accession KM190152
>BB_CT-19
CAGATCCACATGCCCCCAGAATTTGTTGCAGGAATTGCTAGTGGGCAGATCAAGTGGGACAAACCCTTGGAGCTGTCCTGTAATCGTCTGTGGAGGCAGGGAAGCGATTTCCTGTGTCGGGCGTGCTCTCGAAGTAGACCTTCAAATCGAACAGTCTCGGAGAAGGAGGAGGACAAGATGAAAAGAGAGAGAGAGAGAGAGAGAGAGAAGAAGAAGAAGAAGAAGTGGAATTGGTCGCAGTGGGGAGACGGTGGTCAAAATGGTTTACCGTCACAGCGACC
Musa balbisiana ‘Pisung Klutuk Wulung’ sequence at Banana Genome Hub
>chr3:8630981..8631254
GGTCGCTGTGACGGTCAACCATTTTGACCACCGTCTCCCCACTGCGACCAATTCTACTTCTTCTTCTTCTTCTTCTCTCTCTCTCTCTCTCTCTTCGTCTTGTCCTCCTCCTTCTCCGAGACTGTTCGATTTGAAGGTCTACTTAAAGAGCACGCCCGACACAGGAAGCGCTTCCCTGCCTCCACAGACGATTACAGGACAGCTCCAAGGGTTTGTCCCACTTGATCTACCAACTAGCAAAACCTGCAACAAATTCTGGGGGCATGTGGATCTG
CLUSTAL 2.1 multiple sequence alignment
Note: Sequence of BB_CT-19 clone is reverse complemented
BB_CT-19 GGTCGCTGTGACGGTAAACCATTTTGACCACCGTCTCCCCACTGCGACCAATTCCACTTC 60
chr3 GGTCGCTGTGACGGTCAACCATTTTGACCACCGTCTCCCCACTGCGACCAATTCTACTTC 60
*************** ************************************** *****
BB_CT-19 TTCTTCTTCTTCTTCTCTCTCTCTCTCTCTCTCTCTCTTTTCATCTTGTCCTCCTCCTTC 120
chr3 TTCTTCTTCTTCTTCTCTCTCTCTCTCTCTCTCT------TCGTCTTGTCCTCCTCCTTC 114
********************************** ** *****************
BB_CT-19 TCCGAGACTGTTCGATTTGAAGGTCTACTTCGAGAGCACGCCCGACACAGGAAATCGCTT 180
chr3 TCCGAGACTGTTCGATTTGAAGGTCTACTTAAAGAGCACGCCCGACACAGGAAG-CGCTT 173
****************************** ********************* *****
BB_CT-19 CCCTGCCTCCACAGACGATTACAGGACAGCTCCAAGGGTTTGTCCCACTTGATCTGCCCA 240
chr3 CCCTGCCTCCACAGACGATTACAGGACAGCTCCAAGGGTTTGTCCCACTTGATCTACCAA 233
******************************************************* ** *
BB_CT-19 CTAGCAATTCCTGCAACAAATTCTGGGGGCATGTGGATCTG 281
chr3 CTAGCAAAACCTGCAACAAATTCTGGGGGCATGTGGATCTG 274
******* ********************************
Banana SSR markers – Primers Set 2
NED / BB_AAC-9
F: CTAATTTTCCGCTGCCACTG 5’to3’
R: CACGACTGGAAGACGAGTCA 5’to3’
SSR-enriched library clone sequence
Accession KM190153
>BB_AAC-9
CCATCTGAAATTTTTCCTGATAAAAGGATAATCGTCAGATATTTGCACCATGAGATAAATATATGTCCATAATTAGGAAAAACATCTGAATTTCGCTAGACACAAAGGCAACCGCTCAACCAACGTATAAATATATTGACACTGGGCACAATCTTGAAAAGGATCCATTCTCATCAGTTTTCACGAAGAATTCACCAATACAGCCACGTCCGCATCGGGACTTGACCGTCCTAGAGTCCAACAAATGTAAGTTCAACACCTAAATCAGCTGCCATCCCATCATCGACGGAAGGTCCACAAGATCTATGGGAGCAAACGAAGCAGTGAAGGAGAATTGAGCATCGGAAACCATACCGCTCGCTGCCACTCTTCTTTGGGATCTTTATCGACTCGGAAACCCTAATTTTCCGCTGCCACTGGACCCATGCCTGGCTCGCGCCCGATGCCCTCCGCTCCCCGCCGCCGAGGTCTCCGAGAAAGGCAGCCGATTGAGGCGAACGATCGGCGGAGGGCGGGGAAGGAAGGATCGACGGATCGGGCGAGATAGAAGGCTTCGGTATTGGGCGAAGAGAGAGAGAGAGAGAGGCGAATCGTTTGACTCGTCTTCCAGTCGTGGGGCCGTCGAAGGGTGGCAGGAGTTGTACGGCTGCTCGAAGGGGCTTCTGAGAATGAAATAAGAGTGAAGCCGACAATTTCCGTGGGAGGAACAAAAATATAT
Musa balbisiana ‘Pisung Klutuk Wulung’ sequence at Banana Genome Hub
>chr5:7069277..7069992
CCATCTGAAATTTTTCCTGATAAAAGGATAATCGTCTGATATTTGCACCATGAGATAAATATATGTCCATAATTAGGAAAAACATATGAATTTCACTGGACACAAAGGCAACCGCTCAACCAACGTATAAATATATTGACACTGGGCACAATCTTGAAAAGGATCCATTCTCATCAGTTTTCACGAAGAATTCACCAATACAGCCACGTCCGCATCGGGACTTGACCGTCCTAGAGTCCAACAAATGTAAGTTCAACACCTAAATCAGCTGCTATCCCATCATCGACGCAAGGTCCACAAGATCTATGGGAGCAAACGAAGCAGTGAACGAGAATTGAGCATCGGAAACCATACCGCTCGCTGCCACTCTTCTCGGGGATCTTTATCGAGTCGGAAACCCTAATTTTCCGCTGCCACTGGACCCATGCCTGGCTCGCGCCCGATGCCCTCCGCTCCCCGCCGCCGAGGTCTCCGAGAAAGGCGGCCGATTGAGGCGAACGATCGGCGGAGGGCGGGGAAGGAAGGATCGAAGGATCGGGCGAGATAGAAGGTTTCGGTATTGGGCGAAGAGAGAGAGAGAGAGATGAATCGTTTGACTCGTCTTCCAGTCGTGGGGCCGTCCAAGGGTGGCAGATGTTGTACGGCTGCTCGAGGCGGTTTCTGAGAATGAAATAAGAGTGAAGCCGACAATTTCCGTGGGAGGAACAAAAATATAT
CLUSTAL 2.1 multiple sequence alignment
BB_AAC-9 CCATCTGAAATTTTTCCTGATAAAAGGATAATCGTCAGATATTTGCACCATGAGATAAAT 60
chr5 CCATCTGAAATTTTTCCTGATAAAAGGATAATCGTCTGATATTTGCACCATGAGATAAAT 60
************************************ ***********************
BB_AAC-9 ATATGTCCATAATTAGGAAAAACATCTGAATTTCGCTAGACACAAAGGCAACCGCTCAAC 120
chr5 ATATGTCCATAATTAGGAAAAACATATGAATTTCACTGGACACAAAGGCAACCGCTCAAC 120
************************* ******** ** **********************
BB_AAC-9 CAACGTATAAATATATTGACACTGGGCACAATCTTGAAAAGGATCCATTCTCATCAGTTT 180
chr5 CAACGTATAAATATATTGACACTGGGCACAATCTTGAAAAGGATCCATTCTCATCAGTTT 180
************************************************************
BB_AAC-9 TCACGAAGAATTCACCAATACAGCCACGTCCGCATCGGGACTTGACCGTCCTAGAGTCCA 240
chr5 TCACGAAGAATTCACCAATACAGCCACGTCCGCATCGGGACTTGACCGTCCTAGAGTCCA 240
************************************************************
BB_AAC-9 ACAAATGTAAGTTCAACACCTAAATCAGCTGCCATCCCATCATCGACGGAAGGTCCACAA 300
chr5 ACAAATGTAAGTTCAACACCTAAATCAGCTGCTATCCCATCATCGACGCAAGGTCCACAA 300
******************************** *************** ***********
BB_AAC-9 GATCTATGGGAGCAAACGAAGCAGTGAAGGAGAATTGAGCATCGGAAACCATACCGCTCG 360
chr5 GATCTATGGGAGCAAACGAAGCAGTGAACGAGAATTGAGCATCGGAAACCATACCGCTCG 360
**************************** *******************************
BB_AAC-9 CTGCCACTCTTCTTTGGGATCTTTATCGACTCGGAAACCCTAATTTTCCGCTGCCACTGG 420
chr5 CTGCCACTCTTCTCGGGGATCTTTATCGAGTCGGAAACCCTAATTTTCCGCTGCCACTGG 420
************* ************** ******************************
BB_AAC-9 ACCCATGCCTGGCTCGCGCCCGATGCCCTCCGCTCCCCGCCGCCGAGGTCTCCGAGAAAG 480
chr5 ACCCATGCCTGGCTCGCGCCCGATGCCCTCCGCTCCCCGCCGCCGAGGTCTCCGAGAAAG 480
************************************************************
BB_AAC-9 GCAGCCGATTGAGGCGAACGATCGGCGGAGGGCGGGGAAGGAAGGATCGACGGATCGGGC 540
chr5 GCGGCCGATTGAGGCGAACGATCGGCGGAGGGCGGGGAAGGAAGGATCGAAGGATCGGGC 540
** *********************************************** *********
BB_AAC-9 GAGATAGAAGGCTTCGGTATTGGGCGAAGAGAGAGAGAGAGAGAGGCGAATCGTTTGACT 600
chr5 GAGATAGAAGGTTTCGGTATTGGGCGAAGAGAGAGAGAGAGAGA--TGAATCGTTTGACT 598
*********** ******************************** *************
BB_AAC-9 CGTCTTCCAGTCGTGGGGCCGTCGAAGGGTGGCAGGAGTTGTACGGCTGCTCGAAGGGGC 660
chr5 CGTCTTCCAGTCGTGGGGCCGTCCAAGGGTGGCAGATGTTGTACGGCTGCTCGAGGCGGT 658
*********************** *********** ***************** * **
BB_AAC-9 TTCTGAGAATGAAATAAGAGTGAAGCCGACAATTTCCGTGGGAGGAACAAAAATATAT 718
chr5 TTCTGAGAATGAAATAAGAGTGAAGCCGACAATTTCCGTGGGAGGAACAAAAATATAT 716
**********************************************************
Banana SSR markers – Primers Set 2
PET / BB_GAA-31
F: ACCGAAGAAAACGAAGCAGA 5’to3’
R: GACCCTCGGATGTGTGTACC 5’to3’
SSR-enriched library clone sequence
Accession KM190154
>BB_GAA-31
AAGAAGAAATAGGGACTTCAAGAAGAAACAGAAACAGCACAGCTGATCGCGACATAAACCCTAAAGATCGCAACAAAATCCAAGCGTCGAAGAATGGGGAAGAAAGAACTCACACCGAAGAAAACGAAGCAGAAGAGACCCAACGCGAGTACTTTCTTCTACAATTGATTTCTTAGCCCGCGACAGTGTGAGTTTGAGAATAAGCGAAGGAAGAAGAGAAGACGAGGACGGTGAAGAGAGAGAGAGGAGCTATAAATAGGTTAGTATGATCCACGTGGTGGTACACACATCCGAGGGTCATGAATGCGCGAGCGACAATCATCGATGATGATCATCCGTTGATTTGAAGCGAACGGGTACAGCCGCGTCGCGTAGGATTCACGCCAAGCCGGTCCGCCGAGCCATTAGCTCAGATTACATGTACACACCCAAAAACCTGGCTTTTCCTACCCCGGCTTTCGCTACTCCAAATATACCTGGCTTTTTACTGGAGATGGCAATGGGCCACAACAATAAACCTCAAGGTCACTAGCAAGGACGGT
Musa balbisiana ‘Pisung Klutuk Wulung’ sequence at Banana Genome Hub
>chr6:23859599..23860128
ACCGTCCTGGCCAGTGACCTTGAGGTTTATTGTTGTGGCCCAGTGCCATCTCCAGTAAAAAGCCAGGTATCTCTGGAGTAGCAAAAGCCGGGGTAGGAAAAGCCAGGTTTTTGGGTGTGCACATGTAATCTGAGCTAATGGCTCGGCGGACCGGCTCGGCGTGAATCCTAAGCGACGCGGCTATACCCTTTCGCTTCAAATCAACGGACGATCATCATCGATGATTGTCGCTCGCGCATTCATGACCCTCGGATGTGTGTACCACCACGTGGATCATACTAACCTATTTATAGCTCCTCTCTCTCTCTCTCTCTTCATCGTCCTCTTCTCTTCTTCGCTTATTCTCAAACTCACACTCTCGCGGGCTAAGAAATCAATTGTAGAAGAAAGTACTCGCGGTGGGTCTCTTCTGCTGGTGTGAGTTCTTTCTTCCCCGTTCGTGGACGCTCCGATTTTGTTGCGATCTTTAGGATTTATGTCGCGATCAGCTGTGCTGTTTCTGTTTCTTCTTTAAGTCCCTATTTCTTCTT
CLUSTAL 2.1 multiple sequence alignment
Note: Sequence of BB_GAA-31 clone is reverse complemented
BB_GAA-31 ACCGTCCTTGCTAGTGACCTTGAGGTTTATTGTTGTGGCCCATTGCCATCTCCAGTAAAA 60
chr6 ACCGTCCTGGCCAGTGACCTTGAGGTTTATTGTTGTGGCCCAGTGCCATCTCCAGTAAAA 60
******** ** ****************************** *****************
BB_GAA-31 AGCCAGGTATATTTGGAGTAGCGAAAGCCGGGGTAGGAAAAGCCAGGTTTTTGGGTGTGT 120
chr6 AGCCAGGTATCTCTGGAGTAGCAAAAGCCGGGGTAGGAAAAGCCAGGTTTTTGGGTGTGC 120
********** * ********* ************************************
BB_GAA-31 ACATGTAATCTGAGCTAATGGCTCGGCGGACCGGCTTGGCGTGAATCCTACGCGACGCGG 180
chr6 ACATGTAATCTGAGCTAATGGCTCGGCGGACCGGCTCGGCGTGAATCCTAAGCGACGCGG 180
************************************ ************* *********
BB_GAA-31 CTGTACCCGTTCGCTTCAAATCAACGGATGATCATCATCGATGATTGTCGCTCGCGCATT 240
chr6 CTATACCCTTTCGCTTCAAATCAACGGACGATCATCATCGATGATTGTCGCTCGCGCATT 240
** ***** ******************* *******************************
BB_GAA-31 CATGACCCTCGGATGTGTGTACCACCACGTGGATCATACTAACCTATTTATAGCTCCTCT 300
chr6 CATGACCCTCGGATGTGTGTACCACCACGTGGATCATACTAACCTATTTATAGCTCCTCT 300
************************************************************
BB_GAA-31 CTCTCTCTTCACCGTCCTCGTCTTCTCTTCTTCCTTCGCTTATTCTCAAACTCACACTGT 360
chr6 CTCTCTCTCTCTCTTCATCGTCCTCTTCTCTTC-TTCGCTTATTCTCAAACTCACACTCT 359
******** * ** ***** *** ***** ************************ *
BB_GAA-31 CGCGGGCTAAGAAATCAATTGTAGAAGAAAGTACTCGCGTTGGGTCTCTTCTGCTTCGTT 420
chr6 CGCGGGCTAAGAAATCAATTGTAGAAGAAAGTACTCGCGGTGGGTCTCTTCTGCT----- 414
*************************************** ***************
BB_GAA-31 TTCTTCGGTGTGAGTTCTTTCTTCCCCATTCTTCGACGCTTGGATTTTGTTGCGATCTTT 480
chr6 ------GGTGTGAGTTCTTTCTTCCCCGTTCGTGGACGCTCCGATTTTGTTGCGATCTTT 468
********************* *** * ****** ******************
BB_GAA-31 AGGGTTTATGTCGCGATCAGCTGTGCTGTTTCTGTTTCTTCTTGAAGTCCCTATTTCTTC 540
chr6 AGGATTTATGTCGCGATCAGCTGTGCTGTTTCTGTTTCTTCTTTAAGTCCCTATTTCTTC 528
*** *************************************** ****************
BB_GAA-31 TT 542
chr6 TT 530
**
Banana SSR markers – Primers Set 3
FAM / BB_CT-38.1
F: TACTCCCCCACCATAAGAGC 5’to3’
R: CGTTCCTTTGCGTGTCTG5’to3’
SSR-enriched library clone sequence
Accession KM190155
>BB_CT-38.1
TGACCACCAAAGTCATGTAGCATTTAGAAGACTACTCCCCCACCATAAGAGCTCCTTTATCCAATCCTCCATGTCCTATCACACGCATGAATAGAGATATGGCCATATTATATTGCAGCAGTCACTAATTGAGTCTTTGGTGCATTTCTCCTCCTCCTCCACTAGGGGAGAAAGAGTGGGCGCTAAAAGACAGCTGGGTTTGGTGATTATGTTCCGATGCCCTATTAGTTTGCATGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAAGCAGACACGCAAAGGAACGGGAACAACGCAAAGGATTTGACTAGGGCACGGGGGGGTTTGATCACCCCGGGACCCTAAGTGCTATTCTTCCGAGTTG
Musa balbisiana ‘Pisung Klutuk Wulung’ sequence at Banana Genome Hub
>chr9:6707891..6708245
CAACTCGGAAGAATGGCATTTAGGGTCACGGGGTGATCAAACCACCCAGTGCCCTAGTCAATTCCTTTGCGTTGTTCCTGTTCCTTTGCGTGTCTGCTTCTCTCTCTCTCTCTCTCTCTCTCATGCAAACTAATAGGGCATCGGAACATAATCACCAAACCCAGCTGTCTTTTAGCGCCCACTCTTTCTCCTCTAGTGGAGGAGGAGAAATGCAGCAACGACTCAATTAGTGACTGCTGCAATATAATATGGCCATGATCTCTATTCATGCGTGTGATAGGACATGGAGGATTGGATAAAGGAGCTCTTATGGTGGGGGAGTAGTCTTCTAAATGCTACATGACTTTGGTGGTCA
CLUSTAL 2.1 multiple sequence alignment
Note: Sequence of BB_CT-38.1 clone is reverse complemented
BB_CT-38.1 CAACTCGGAAGAATAGCACTTAGGGTCCCGGGGTGATCAAACCCCCCCGTGCCCTAGTCA 60
chr9 CAACTCGGAAGAATGGCATTTAGGGTCACGGGGTGATCAAACCACCCAGTGCCCTAGTCA 60
************** *** ******** *************** *** ************
BB_CT-38.1 AATCCTTTGCGTTGTTCCCGTTCCTTTGCGTGTCTGCTTCTCTCTCTCTCTCTCTCTCTC 120
chr9 ATTCCTTTGCGTTGTTCCTGTTCCTTTGCGTGTCTGCTTCTCTCTCTCTCTCTCT----- 115
* **************** ************************************
BB_CT-38.1 TCTCTCTCTCTCATGCAAACTAATAGGGCATCGGAACATAATCACCAAACCCAGCTGTCT 180
chr9 -----CTCTCTCATGCAAACTAATAGGGCATCGGAACATAATCACCAAACCCAGCTGTCT 170
*******************************************************
BB_CT-38.1 TTTAGCGCCCACTCTTTCTCCCCTAGTGGAGGAGGAGGAGAAATGCACCAAAGACTCAAT 240
chr9 TTTAGCGCCCACTCTTTCTCCTCTAGTGGAGGAGGAG---AAATGCAGCAACGACTCAAT 227
********************* *************** ******* *** ********
BB_CT-38.1 TAGTGACTGCTGCAATATAATATGGCCAT-ATCTCTATTCATGCGTGTGATAGGACATGG 299
chr9 TAGTGACTGCTGCAATATAATATGGCCATGATCTCTATTCATGCGTGTGATAGGACATGG 287
***************************** ******************************
BB_CT-38.1 AGGATTGGATAAAGGAGCTCTTATGGTGGGGGAGTAGTCTTCTAAATGCTACATGACTTT 359
chr9 AGGATTGGATAAAGGAGCTCTTATGGTGGGGGAGTAGTCTTCTAAATGCTACATGACTTT 347
************************************************************
BB_CT-38.1 GGTGGTCA 367
chr9 GGTGGTCA 355
********
Banana SSR markers – Primers Set 3
HEX / BB_CT-11
F: GGCTGTACTCCTGTGGTGGT 5’to3’
R: TGTCACCAATCCATGACCAG 5’to3’
SSR-enriched library clone sequence
Accession KM190156
>BB_CT-11
CACAACCAGACACACACAAACACACAGAGAGAAGCAGCAGTCTCTTCGTACTCTTGTTGAGAACAAAGAGCATACAAGAGAAGCATTTCAGAGAGAAGTGGACGGCAGAGGAAGAGGAAGAGGAGGAGGAGGTGATCGTAGGAGAGGAGTGTTTATAGAATGGATGGTCAGAGTGCAACCAGTACTGGATTCAAACAAAAGGTGAAGGCTGTACTCCTGTGGTGGTGATGGCACACTAGCATATGATGAGCTTTTGGCATCTCTTTCTTTGTGGGCATGGGAGGTGAATGAAGCGTGAGATCTATGAACAGCTTTCCCTTACTTTATCTCTCTCTCTCTCTCTTCTTTTTTGCCTGAGTACAGTATTCTTTATTTCTAGCATCTCTGGTCATGGATTGGTGACAGGTTTGTCTCTTTGACCGAGTTCTTCGACGTCTCTCTGGTTGGTTGCTGTGGTCTTCCACATGGGAGACCCCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTAGCCGGATGAAGGAGGAATAGTGGGCCAAAATGCTTCTGAAGAAAAAGAAGTAGAGTTATCGTGGCTTGA
Musa balbisiana ‘Pisung Klutuk Wulung’ sequence at Banana Genome Hub
>chr6:5004067..5004676
CACAACCAGACACACACAAACACACAGAGAGAAGCAGCAGTCTCTTCGTACTCTTGTTGAGAACAAAGAGCATACAAGAGAAGCATTTCAGAGAGAAGTGGACGGCAGAGGAAGAGGAGGAGGAGGAGGAGAGGAGTGTTTATAGAATGGATGGTCAGAGTGCAACCAGTACTGGATTCAAACAAAAGGTGAAGGCTGTACTCCTGTGGTGGTGATGGCACACTAGCATATGATGAGCTTTTGGCATCTCTTTCTTTGTGGGCATGGGAGGTGAATGAAGCGTGAGATCTATGAACAGCTTTCCCTTACTTTATCTCTCTCTCTCTCTCTCTCTCTCTCTTCTTTTTTGCCTGAGTACAGTATTCTTTATTTCTAGCATCTCTGGTCATGGATTGGTGACAGGTTTGTCTCTTTGACCGAGTTCTTCGACGTCTCTCTGGTTGGTTGCTGTGGTCTTCCACATGGGAGACCTCTCTCTCTCTCCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTAGCCGGATGAAGGAGGAGGAATAGTGGGCCAAAATGCTTCTGAAGAAAAAGAAGTAGAGTTATCGTGGCTTGA
CLUSTAL 2.1 multiple sequence alignment
BB_CT-11 CACAACCAGACACACACAAACACACAGAGAGAAGCAGCAGTCTCTTCGTACTCTTGTTGA 60
chr6 CACAACCAGACACACACAAACACACAGAGAGAAGCAGCAGTCTCTTCGTACTCTTGTTGA 60
************************************************************
BB_CT-11 GAACAAAGAGCATACAAGAGAAGCATTTCAGAGAGAAGTGGACGGCAGAGGAAGAGGAAG 120
chr6 GAACAAAGAGCATACAAGAGAAGCATTTCAGAGAGAAGTGGACGGCAGAGGAAGAGGAGG 120
********************************************************** *
BB_CT-11 AGGAGGAGGAGGTGATCGTAGGAGAGGAGTGTTTATAGAATGGATGGTCAGAGTGCAACC 180
chr6 AGGAGGAGGAG------AGGAGTGTTTATAGAATGGATGGTCAGAGTGCAACC 167
*********** ************************************
BB_CT-11 AGTACTGGATTCAAACAAAAGGTGAAGGCTGTACTCCTGTGGTGGTGATGGCACACTAGC 240
chr6 AGTACTGGATTCAAACAAAAGGTGAAGGCTGTACTCCTGTGGTGGTGATGGCACACTAGC 227
************************************************************
BB_CT-11 ATATGATGAGCTTTTGGCATCTCTTTCTTTGTGGGCATGGGAGGTGAATGAAGCGTGAGA 300
chr6 ATATGATGAGCTTTTGGCATCTCTTTCTTTGTGGGCATGGGAGGTGAATGAAGCGTGAGA 287
************************************************************
BB_CT-11 TCTATGAACAGCTTTCCCTTACTTTATCTCTCTCTCTCTCTCT------TCTTTTT 350
chr6 TCTATGAACAGCTTTCCCTTACTTTATCTCTCTCTCTCTCTCTCTCTCTCTCTTCTTTTT 347
******************************************* *******
BB_CT-11 TGCCTGAGTACAGTATTCTTTATTTCTAGCATCTCTGGTCATGGATTGGTGACAGGTTTG 410
chr6 TGCCTGAGTACAGTATTCTTTATTTCTAGCATCTCTGGTCATGGATTGGTGACAGGTTTG 407
************************************************************
BB_CT-11 TCTCTTTGACCGAGTTCTTCGACGTCTCTCTGGTTGGTTGCTGTGGTCTTCCACATGGGA 470
chr6 TCTCTTTGACCGAGTTCTTCGACGTCTCTCTGGTTGGTTGCTGTGGTCTTCCACATGGGA 467
************************************************************
BB_CT-11 GACCCCTCTCTCTCTC------TCTCTCTCTCTCTCT 501
chr6 GACCTCTCTCTCTCTCCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT 527
**** *********** ***************
BB_CT-11 CTCTCTCTCTAGCCGGATGAAGGAGGA---ATAGTGGGCCAAAATGCTTCTGAAGAAAAA 558
chr6 CTCTCTCTCTAGCCGGATGAAGGAGGAGGAATAGTGGGCCAAAATGCTTCTGAAGAAAAA 587
*************************** ******************************
BB_CT-11 GAAGTAGAGTTATCGTGGCTTGA 581
chr6 GAAGTAGAGTTATCGTGGCTTGA 610
***********************
Banana SSR markers – Primers Set 3
NED / BB_CT-37
F: TGTTGGATTGCCATGGTATG 5’to3’
R: TCCGAGTCTACTCCCAAGGA 5’to3’
SSR-enriched library clone sequence
Accession KM190157
>BB_CT-37
TGGTTTGTTGGATTGCCATGGTATGCTGGTTTGTTCTGGGAGTACTAACGGTACAAAAGGAAAACAATTAGATGAAATGTGTGTGTGTGTGTGTGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGATTGCAAGATTTCCTCCTCTTTGTCTTTTCTCCTTTTTGTAGTGTTCCTTGGGAGTAGACTCGGAGATTGTGTCCTAATCATGTGATTGAAAAAGTTGATTAGTTTGGTCAAGGCTTGTTCAGTTTGGCTTTGGGTCGAGAAATGTCAGGGTTAGAATGATTTTGGTTGAAACTTTTGTTTTTCAAAAGCAACCTGTTGTTTCAAAGGTTCGACTGAAGTAGGGCAGAATTTGTATACTTGAAATAGTCAAAATTCCATGAATCAGAATTCC
Musa balbisiana ‘Pisung Klutuk Wulung’ sequence at Banana Genome Hub
>chr11:8703745..8704129
GGAATTCTGATTCATGGAATTTTGACTATTTCAAGTATACAAATTCTGCCCTACTTCAGTCGAACCTTTGAAACAACAGGTTGCTTTTGAAAAACAAAAGTTTCAACCAAAATCATTCTAACCCTGACATTTCTCGACCCAAAGCCAAACTGAACAAGCCTTGACCAAACTAATCAACTTTTTCAATCACATGATTAGGACACAATCTCCGAGTCTACTCCCAAGGAACACTACAAAAAGGAGAAAAGACAAAGAGGAGGAAATCTTGCAATCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCACATTTCATCTAATTGTTTTCCTTTTGTACCGTTAGTACTCCCAGAACAAACCAGCATACCATGGCAATCCAACAAACCA
CLUSTAL 2.1 multiple sequence alignment
Note: Sequence of BB_CT-37 clone is reverse complemented
BB_CT-37 GGAATTCTGATTCATGGAATTTTGACTATTTCAAGTATACAAATTCTGCCCTACTTCAGT 60
chr11 GGAATTCTGATTCATGGAATTTTGACTATTTCAAGTATACAAATTCTGCCCTACTTCAGT 60
************************************************************
BB_CT-37 CGAACCTTTGAAACAACAGGTTGCTTTTGAAAAACAAAAGTTTCAACCAAAATCATTCTA 120
chr11 CGAACCTTTGAAACAACAGGTTGCTTTTGAAAAACAAAAGTTTCAACCAAAATCATTCTA 120
************************************************************
BB_CT-37 ACCCTGACATTTCTCGACCCAAAGCCAAACTGAACAAGCCTTGACCAAACTAATCAACTT 180
chr11 ACCCTGACATTTCTCGACCCAAAGCCAAACTGAACAAGCCTTGACCAAACTAATCAACTT 180
************************************************************
BB_CT-37 TTTCAATCACATGATTAGGACACAATCTCCGAGTCTACTCCCAAGGAACACTACAAAAAG 240
chr11 TTTCAATCACATGATTAGGACACAATCTCCGAGTCTACTCCCAAGGAACACTACAAAAAG 240
************************************************************
BB_CT-37 GAGAAAAGACAAAGAGGAGGAAATCTTGCAATCTCTCTCTCTCTCTCTCTCTCTCTCTCT 300
chr11 GAGAAAAGACAAAGAGGAGGAAATCTTGCAATCTCTCTCTCTCTCTCTCTCTCTCTCTCT 300
************************************************************
BB_CT-37 CTCTCTCTCTCTCACACACACACACACACATTTCATCTAATTGTTTTCCTTTTGTACCGT 360
chr11 CTCTC------ACATTTCATCTAATTGTTTTCCTTTTGTACCGT 338
***** *********************************
BB_CT-37 TAGTACTCCCAGAACAAACCAGCATACCATGGCAATCCAACAAACCA 407
chr11 TAGTACTCCCAGAACAAACCAGCATACCATGGCAATCCAACAAACCA 385
***********************************************
Banana SSR markers – Primers Set 3
PET / BB_CT-39
F: TTGAGCCTATGATGGGGATG 5’to3’
R: TTGGAGTTTGCCTCTCTCAC 5’to3’
SSR-enriched library clone sequence
Accession KM190158
>BB_CT-39
CGCATTGAGCCTATGATGGGGATGGGGCTGATATTGAGGTCAAGTGGAGACAAAACCAATTCGTTCTTTGGTGCCCAACATGCTTGTTCTCAGGCTAAACATTGCCAAGGCTTGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGTGAGAGAGGCAAACTCCAATAACTTTCCCCACTATTTTTCTTTCTTCCCATATTTCTTTCATTTTGTTTTTTTTTTTCTTCTTTTCATTTTGGTTT
Musa balbisiana ‘Pisung Klutuk Wulung’ sequence at Banana Genome Hub
>chr3:15301070..15301295
AAACCAAAATGAAAAGAAGGAAAAAAAACAAAATGAAAGAAATATGGAAAGAAAGAAAAATAGTGGGGAAAGTTATTGGAGTTTGCCTCTCTCTCTCTCTCTCTCTCTCTCTCTAGCCTTGGCAATGTTTAGCCTGAGAACAAGCATGTTGGGCACCAAAGAACGAATTGGTTTTGTCTCCACTTGACCTCAATATCAGCCCCATCCCCATCATAGGCTCAATGCG
CLUSTAL 2.1 multiple sequence alignment
Note: Sequence of BB_CT-39 clone is reverse complemented
BB_CT-39 AAACCAAAATGAAAAGAAGAAAAAAAAAAACAAAATGAAAGAAATATGGGAAGAAAGAAA 60
chr3 AAACCAAAATGAAAAGAAGGAAAAAAAA--CAAAATGAAAGAAATATGGAAAGAAAGAAA 58
******************* ******** ******************* **********
BB_CT-39 AATAGTGGGGAAAGTTATTGGAGTTTGCCTCTCTCACTCTCTCTCTCTCTCTCTCTCTCT 120
chr3 AATAGTGGGGAAAGTTATTGGAGTTTGCCTCTCT------92
**********************************
BB_CT-39 CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCAAGCCTTGGCAATGTTTAGCCTGAG 180
chr3 ------CTCTCTCTCTCTCTCTCTCTCTAGCCTTGGCAATGTTTAGCCTGAG 138
********************* ************************
BB_CT-39 AACAAGCATGTTGGGCACCAAAGAACGAATTGGTTTTGTCTCCACTTGACCTCAATATCA 240
chr3 AACAAGCATGTTGGGCACCAAAGAACGAATTGGTTTTGTCTCCACTTGACCTCAATATCA 198
************************************************************
BB_CT-39 GCCCCATCCCCATCATAGGCTCAATGCG 268
chr3 GCCCCATCCCCATCATAGGCTCAATGCG 226
****************************
Banana SSR markers – Primers Set 4
FAM / BB_CT-2
F: TTGTTTTGCTGATGCTGACC 5’to3’
R: GCGATAACATTCTCCGCAAT 5’to3’
SSR-enriched library clone sequence
Accession KM190159
>BB_CT-2
TGCAGCCAAATCGGAAAGAAGCTCGTACCTGTGGGATCATAACTTTGTTTTGCTGATGCTGACCATACAACGAGAGTTGGGGTGATGATGGCGAGCAGAGCCCAGAAGAAGAAGATCAGAGCGTAGCCTCTAGGAGGCATATCTCAACTGTACAAGAAGAAACGGGAGCTTTTTGGGAAGAAGGAAGGAAAGAGAGAGAGAGAGAGAGAGAAAATCCGAGGCCACTGATATGTCGATCGACACGTCTATTGGACTCCAGAAATTGCTCGGATTGCGGAGAATGTTATCGCAAATACTCAAACAAATAAAGCCATTTGGCTTTCTGGTCAGCTACGTGATCGCAATTCTCTACACGACTCATTCCGTAGTGTTGCGCATGTCTCAGGGATATAAGTCCGCTGTGTCGTGCGTGTCACCTTTGTCCATCTCGGG
Musa balbisiana ‘Pisung Klutuk Wulung’ sequence at Banana Genome Hub
>chr4:19640163..19640585
TGTCCTCGAGGAAATCATATGATCTCACCTGTGGTGTGGTGACTTTGTTTTCCTGAAGCTGACAGGAAGATGAGAGTCGGGGTGATGACGGTGAGCAGAGCCCAGAAGAAGAAGATCAAAGCGTAACCTCTAGGAGGCATATCTCAATTGTCCTATAAGCAATGGGAGCGGTTCCGGAATCTCTCTTCGGAAGGAGGGAGAAAGAGAAAGGAATAACTCGATGGCAGAGGAGCAGAGAAGGAGGGACAACTGAAAACTGTAGACAGGAGGAACTAATCTCGTTTTTCTCTATGAATTCTAGCATGGCAGGTCACAAACACTTGTAATAAACTAATGAGGAGGAGGTTTGTTGATGAGTAGGTGAGAGACTGGTGAAGGCAGATGTTGACTGAGAGGAAACTCTTCTCCCGTATTTAGCGGGGA
CLUSTAL 2.1 multiple sequence alignment
BB_CT-2 TGCAGCCAAATCGGAA--AGAAGCTCGTACCTGTGGGATCATAACTTTGTTTTGCTGATG 58
chr4 TGTCCTCGAG--GAAATCATATGATCTCACCTGTGGTGTGGTGACTTTGTTTTCCTGAAG 58
** * * * ** * * * ** ******** * * ********** **** *
BB_CT-2 CTGACCATACAACGAGAGTTGGGGTGATGATGGCGAGCAGAGCCCAGAAGAAGAAGATCA 118
chr4 CTGACAGGAAGATGAGAGTCGGGGTGATGACGGTGAGCAGAGCCCAGAAGAAGAAGATCA 118
***** * * ****** ********** ** **************************
BB_CT-2 GAGCGTAGCCTCTAGGAGGCATATCTCAACTGTACAAGAAGAAACGGGAGCTTTTTGGGA 178
chr4 AAGCGTAACCTCTAGGAGGCATATCTCAATTGTCCTATAAGCAATGGGAGCGGTTCCGGA 178
****** ********************* *** * * *** ** ****** ** ***
BB_CT-2 A------GAAGGAAGGAAAGAGAGAGAG------AGAGAGAGAGAAA 213
chr4 ATCTCTCTTCGGAAGGAGGGAGAAAGAGAAAGGAATAACTCGATGGCAGAGGAGCAGAGA 238
* ****** *** * ***** ** **** *** *
BB_CT-2 ATCCGAGGCCACTGATA--TGTCGATCG-----ACACGTCT-ATTGGACTCCAGAAATTG 265
chr4 AGGAGGGACAACTGAAAACTGTAGACAGGAGGAACTAATCTCGTTTTTCTCTATGAATT- 297
* * * * ***** * *** ** * ** *** ** *** * ****
BB_CT-2 CTCGGATTGCGGAGAATGTTATCGCAAATACTC-----AAACAAATAAAGCCATTTGGCT 320
chr4 CTAGCATGGCAGG------TCACAAACACTTGTAATAAACTAATGAGG--AGGAGGTT 347
** * ** ** * ** **** *** **** *** * * * ** *
BB_CT-2 TTCTGGTCAGCTACGTGATCGCAATTCTCTACACGACTCATTCCGTAGTGTTGCGCATGT 380
chr4 TGTTGATGAG-TAGGTGA------GAGACTGGT---GAAG----GCAGATGT 385
* ** * ** ** **** **** * * ** ** ****
BB_CT-2 CT-CAGGGATATAAGTCCGCTGTGTCGTGCGTGTCACCTTTGTCCATCTCGGG 432
chr4 TGACTGAGAGGAAACTCTTCT------CCCGTATTTAGCGGGGA 423
* * ** ** ** ** ** * * * * **
Banana SSR markers – Primers Set 4
HEX / BB_CT-15
F: CACCATTTGTGATGCCACTC 5’to3’
R: TAGGCCACATACCCAGCTTC 5’to3’
SSR-enriched library clone sequence
Accession KM190160
>BB_CT-15
TGATATGTTCATGTGAATTTTAAGAAGTTCAAAAATGCTGTTTCCCCAGGATTTGATCCTGATGTTGACTTAATTAAAGTAGGCATTGCAAATCAGACTACAATGCTCAAAGGTGAAACGGAAGAGATTGGTGAGTTACATGCATCTAATGCTTACTAATTCCTTCTACCATTTCCTGTTTCCAGTCAGATATAATGCTTTGAGATTTCAGGTAAATTGGTTGAGAAGACCATGATGCGAAGATTTGGAATAGAGAACGTCAATGAGCACTTCATTAGCTTCAACACCATTTGTGATGCCACTCAGGTAACTTTACTAAAATCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTGAGGGGTAACTTTCCTGAAATATGTTGTGCTGATGTGGTAGTTGCTTTAGCGACAAGGAACTTGTGCTAGATAATATTTACTGACTTTTTCCCAGATGCCTACACAAAAGTTTCGTTATGTTTTGCCAAGGAAGCTGGGTATGTGGCCTATATTTTTATAGTAAAGACAACTAATTGGTCAAATGAGCATGAGATACACTTTGTTGTAACCGGGATACAGCTTTGTTTCTATATGGATACAATATCCACGAATGACCATAGCTCAGATATTGATCGACAAACGTTGTGCACCAATTTTGCTTGTTGCAGGCCTAACTTCCAACGATACCCACTAGGTCTTGGATTGAGCATATGATTTGTTGCACTTCATA
Musa balbisiana ‘Pisung Klutuk Wulung’ sequence at Banana Genome Hub
>chr8:2868300..2869030
TGATATGTTCATGTGAATTTTACAGAAGTTCAAAAATGCTGTTTCCCCAGGATTTGATCCTGATGTTGACTTAGTTAAAGTAGGCATTGCAAATCAGACTACAATGCTCAAAGGTGAAACGGAAGAGATTGGTGAGTTACATGCATCTAATGCTTACTAATTCCTTCTACCTTTTCCTGTTTCCAGTCAGATATAATGCTTTGAGATTTCAGGTAAATTGGTTGAGAAGACCATGATGCGAAGATTTGGAATAGAGAACGTCAATGAGCACTTCATTAGCTTCAACACCATTTGTGATGCCACTCAGGTAACTTTACTAAAATCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTGAGGGGTAACTTTCCTGAAATATGTTGTTCTGATGTGGTAGTTGCTTTAGCGACAAGGAACTTGTGCTAGATAATATTTACTGACTTTTTCCCAGATGCCTAGACAAAAGTTTCGTTATGTTTTGCCAAGGAAGCTGGGTATGTGGCCTATAGTTTTATAGTAAAGACAACTAATTGGTCAAATGAGCATGAGATACACTTGTTGTAGCCGGGATACAGCTTTGTTCTATATGGATACAATATCCAGGAATGGCCATAGCTCAGATATTGTTCGACAACGTTGTGCACCAATTTGGCTTGTTGCAGGCCTAGCTCCAAGGATATCCACTAGGTCTTGGATTGAGCATATGATTTGTTGCAACTTCATA
NCBI GenBank Database
JQ922096Hordeum chilense isolate H1 4-hydroxy-3-methylbut-2-enyl
diphosphate reductase (Hdr) gene, partial cds.
CLUSTAL 2.1 multiple sequence alignment
BB_CT-15 TGATATGTTCATGTGAATTTTA-AGAAGTTCAAAAATGCTGTTTCCCCAGGATTTGATCC 59
chr8 TGATATGTTCATGTGAATTTTACAGAAGTTCAAAAATGCTGTTTCCCCAGGATTTGATCC 60
JQ922096 ------
BB_CT-15 TGATGTTGACTTAATTAAAGTAGGCATTGCAAATCAGACTACAATGCTCAAAGGTGAAAC 119
chr8 TGATGTTGACTTAGTTAAAGTAGGCATTGCAAATCAGACTACAATGCTCAAAGGTGAAAC 120
JQ922096 ------GGCATTGCCAATCAAACAACAATGCTTAAAGGAGAAAC 38
******** ***** ** ******** ***** *****
BB_CT-15 GGAAGAGATTGGTGAGTTACATGCATCTA--ATGCTTAC-TAATTCCTTCTACCA----T 172
chr8 GGAAGAGATTGGTGAGTTACATGCATCTA--ATGCTTAC-TAATTCCTTCTACCT----T 173
JQ922096 TGAAGAAATTGGTGCGTTTTAC-TATTCATTATGTCTGTGTGATTTTGTACAGTACAGAT 97
***** ******* *** * ** * *** * * *** * * *
BB_CT-15 TTCCTGTT---TCCAGTC--AGATATAATGCTTTGAGATTT-CAGGTAAATTGGTTGAGA 226
chr8 TTCCTGTT---TCCAGTC--AGATATAATGCTTTGAGATTT-CAGGTAAATTGGTTGAGA 227
JQ922096 TTGCTGTTAGTTCCAGTTTTGACTAAAATGCTTTATAATTAACAGGAAAACTTGTTGAGA 157
** ***** ****** ** ******** *** **** *** * *******
BB_CT-15 AGACCATGATGCGAAGATTTGGAATAGAGAACGTCAATGAGCACTTCATTAGCTTCAACA 286
chr8 AGACCATGATGCGAAGATTTGGAATAGAGAACGTCAATGAGCACTTCATTAGCTTCAACA 287
JQ922096 AGACTATGATGCGTAAGTATGGAGTCGAGAATGCCAATGAACACTTTGTTGCCTTCAACA 217
**** ******** * * **** * ***** * ****** ***** ** ********
BB_CT-15 CCATTTGTGATGCCACTCAGGTA-ACTTTACTAAAATCTCTCTCTCTCTCTCTCTCTCTC 345
chr8 CCATTTGTGATGCCACTCAGGTA-ACTTTACTAAAATCTCTCTCTCTCTCTCTCTCTCTC 346
JQ922096 CTATTTGTGATGCCACACAGGTACATTTTCTTATAAGCATATCCTTCCGTTTTATGGAGA 277
* ************** ****** * *** ** ** * ** * * * *
BB_CT-15 TCTCTCTCTCTCTCTCTGAGGGGTAACTTT--CCTGAAATA-----TGTTGTGCTGATGT 398
chr8 TCTCTCTCTCTCTCTCTGAGGGGTAACTTT--CCTGAAATA-----TGTTGTTCTGATGT 399
JQ922096 TTGTGCACTGCATGACTACATGTTATCATTGACCTGTACTACCAACTACTCTGTCAATGC 337
* * ** * ** * ** * ** **** * ** * * * ***
BB_CT-15 GGTAGTTGC------TTTAGCGACAAGGAACTTGTGCTAGATAATATTTACTGACTTTTT 452
chr8 GGTAGTTGC------TTTAGCGACAAGGAACTTGTGCTAGATAATATTTACTGACTTTTT 453
JQ922096 TGCACGTGAGATAAATTCAGTGAGAGCAGAG--GGGC--AATATCATCTTTTGTCCTTTT 393
* * ** ** ** ** * * * ** *** ** * ** * ****
BB_CT-15 CCCAGATGCCTACA-CAAAAGTTTCGTTATGTTTTGCCAAGGAAGCTGGGTATGTGGCCT 511
chr8 CCCAGATGCCTAGA-CAAAAGTTTCGTTATGTTTTGCCAAGGAAGCTGGGTATGTGGCCT 512
JQ922096 ATCTGTTGGGTGGAACTAAAACATAG--ATCTTTTAGC------CTGGTTGGATAGT-T 443
* * ** * * * *** * * ** **** * **** * * * *
BB_CT-15 ATATTTTTATAGTAAAGACAACTA-ATTGGT----CAAATGA------GCATGAGATA 558
chr8 ATAGTTTTATAGTAAAGACAACTA-ATTGGT----CAAATGA------GCATGAGATA 559
JQ922096 GCACTTTTGTAGTAAAGGGAAACATAGTGGTATTTTACTTTGTTTTTATTGCCTAAAAAA 503
* **** ******** ** * * **** * * ** * * * *
BB_CT-15 CACTTTGTT-GTAACCGGG--ATACAGCTTTGTTTCTATATGGATACA-ATATCCACGAA 614
chr8 CACTT-GTT-GTAGCCGGG--ATACAGCTTTGTT-CTATATGGATACA-ATATCCAGGAA 613
JQ922096 TGTTTACTTAGGAGTAAGGTCATAGAGTCGTATCTTCACTTGAACATATATACCGATGAC 563
** ** * * ** *** ** * * * ** * * * *** * * **
BB_CT-15 TGACCATAGCT-CAGATATT--GATCGACAAA-CGTTGTGCACCA------655
chr8 TGGCCATAGCT-CAGATATT--GTTCGACAA--CGTTGTGCACCA------653
JQ922096 ACACTTCAACTTCATATCTCAGGAAAGACAAGATGCGATGTATCACCTAGTGAAGGAAAA 623
* * ** ** ** * * ***** * ** * **
BB_CT-15 ------ATTTTGCTTGTTGCAGGCCTAACTTCCAACGATACC---CACT----AGG 698
chr8 ------ATTTGGCTTGTTGCAGGCCTAGCT-CCAAGGATATC---CACT----AGG 695
JQ922096 GGTTGACCTTATTCTGGTTATTGGAGGGTGGAACTCCAGTAATACCTCTCATTTGCAAGA 683
*** * ** *** *** *** *** * ** * **
BB_CT-15 TCTTGGA---TTGAGCATA----TGATTTGTTGCA-CTTCATA------733
chr8 TCTTGGA---TTGAGCATA----TGATTTGTTGCAACTTCATA------731
JQ922096 AATTGGAGAACTCAGTGGAATTCCGTCCTACTGGA--TTGACAGTGAACAAAGGATTGAC 741
***** * ** * * * ** * ** * *
BB_CT-15 -----
chr8 -----
JQ922096 CCCAG 746
Banana SSR markers – Primers Set 4
NED / BB_CT-35.1
F: CTGCTTCAGTGAGACCCACA 5’to3’
R: ACTAGCAGAAGCGCAGGAAA 5’to3’
SSR-enriched library clone sequence
Accession KM190161
>BB_CT-35.1
AGAAAAGCTTGGAGTTTGAGGGCTTTGCTGCTTCAGTGAGACCCACATCATGCTGAGAGCTCACAAAAAGCCGGGGAGAGAGAGAGAGCGAGAGAGAGAGAGTGGGAGAGGGAAAAGGAAAAGCTAAAAAGTTTGCATCGCCAAGCCAGGATCCTTTGCTTTCTCCCGCTTTGTTATTATATCTGCATGCAATCACCACGGCATGCTGCTTTTCCTGCGCTTCTGCTAGTTTATTTGCATGCAATCCATAGCCCCACTACAAAACCATGAGAACCCCTTTGCGCCATGTTCTGATCCTGGGTCGAAGGTTGCAGGTTGAAAGGGAGTCAGGAGACGGATGCTAAAGAAAGACAGGATAGAGATGGAGAAGAATGCGTTGGTCGGGGGGGGAGAAGAAGAAGAAGAACATGAAGACAGATGTAGGGAATACAGAAAAGGGAACTGGAGTCTTACTGAGACCATGGTTCTGATCGAGGCGAAGAAGATGGATACGAGAGAAGGGCGCAGAGGTTGAAGGAGTTGCCGCGGGGCAGCAGCGGCATCGGCAGTAGCAGGCCACAAGAGATGAGGTGGAAGTGGGTGGAGGACTGTTGCTGGCGACATGGGTGCTACAGGAGCCAAAACCAGTGCAATGACCGGTGGGATAATCTCATGAGGGATTACAAGAAGGTCAGAACATATGAGCTGAGCTTGAGCGCCGGAGGAAGCCCAGGTTTATCTTACTGGAAGCTCGAGAGGCATGAGAGGAAGGAGAAGAACCTGCCCTCCAATCTCTTGCCTGAGATATACGAAGCC
Musa balbisiana ‘Pisung Klutuk Wulung’ sequence at Banana Genome Hub
>chr4:23678379..23679174
AGAAAAGCTTGGAGTTTGAGGGCTTTGCTGCTTCAGTGAGACCCACATCATGCTGAGAGCTCACAAAAAGCCGGGGAGAGAGAGAGAGCGAGAGAGAGAGAGTGGGAGAGGGAAAAGGAAAAGCTAAAAAGTTTGCATCGCCAAGCCAGGATCCTTTGCTTTCTCCCGCTTTGTTATTATATCTGCATGCAATCACCACGGCATGCTGCTTTTCCTGCGCTTCTGCTAGTTTATTTGCATGCAATCCATAGCCCCACTACAAAACCATGAGAACCCCTTTGCGCCATGTTCTGATCCTGGGTCGAAGGTTGCAGGTTGAAAGGGAGTCAGGAGACGGATGCTAAAGAAAGACAGGATAGAGATGGAGAAGAATGCGTTGGTCGGGGGGGGAGAAGAAGAAGAAGAACATGAAGACAGATGTAGGGAATACAGAAAAGGGAACTGGAGTCTTACTGAGACCATGGTTCTGATCGAGGCGAAGAAGATGGATTACGAGAGAAGGGCGCAGAGGTTGAAGGAGTTGCCGCGGGGCAGCAGCGGCATCGGCAGTAGCAGGCCACAAGAGATGAGGTGGAAGTGGGTGGAGGACTGTTGCTGGCGACATGGGTGCTACAGGAGCCAAAACCAGTGCAATGACCGGTGGGATAATCTCATGAGGGATTACAAGAAGGTCAGAACATATGAGCTGAGCTTGAGCGCCGGAGGAAGCCCAGGTTTATCTTACTGGAAGCTCGAGAGGCATGAGAGGAAGGAGAAGAACCTGCCCTCCAATCTCTTGCCTGAGATATACGAAGCC
CLUSTAL 2.1 multiple sequence alignment
BB_CT-35.1 AGAAAAGCTTGGAGTTTGAGGGCTTTGCTGCTTCAGTGAGACCCACATCATGCTGAGAGC 60
chr4 AGAAAAGCTTGGAGTTTGAGGGCTTTGCTGCTTCAGTGAGACCCACATCATGCTGAGAGC 60
************************************************************
BB_CT-35.1 TCACAAAAAGCCGGGGAGAGAGAGAGAGCGAGAGAGAGAGAGTGGGAGAGGGAAAAGGAA 120
chr4 TCACAAAAAGCCGGGGAGAGAGAGAGAGCGAGAGAGAGAGAGTGGGAGAGGGAAAAGGAA 120
************************************************************
BB_CT-35.1 AAGCTAAAAAGTTTGCATCGCCAAGCCAGGATCCTTTGCTTTCTCCCGCTTTGTTATTAT 180
chr4 AAGCTAAAAAGTTTGCATCGCCAAGCCAGGATCCTTTGCTTTCTCCCGCTTTGTTATTAT 180
************************************************************
BB_CT-35.1 ATCTGCATGCAATCACCACGGCATGCTGCTTTTCCTGCGCTTCTGCTAGTTTATTTGCAT 240
chr4 ATCTGCATGCAATCACCACGGCATGCTGCTTTTCCTGCGCTTCTGCTAGTTTATTTGCAT 240
************************************************************
BB_CT-35.1 GCAATCCATAGCCCCACTACAAAACCATGAGAACCCCTTTGCGCCATGTTCTGATCCTGG 300
chr4 GCAATCCATAGCCCCACTACAAAACCATGAGAACCCCTTTGCGCCATGTTCTGATCCTGG 300
************************************************************
BB_CT-35.1 GTCGAAGGTTGCAGGTTGAAAGGGAGTCAGGAGACGGATGCTAAAGAAAGACAGGATAGA 360
chr4 GTCGAAGGTTGCAGGTTGAAAGGGAGTCAGGAGACGGATGCTAAAGAAAGACAGGATAGA 360
************************************************************
BB_CT-35.1 GATGGAGAAGAATGCGTTGGTCGGGGGGGGAGAAGAAGAAGAAGAACATGAAGACAGATG 420
chr4 GATGGAGAAGAATGCGTTGGTCGGGGGGGGAGAAGAAGAAGAAGAACATGAAGACAGATG 420
************************************************************
BB_CT-35.1 TAGGGAATACAGAAAAGGGAACTGGAGTCTTACTGAGACCATGGTTCTGATCGAGGCGAA 480
chr4 TAGGGAATACAGAAAAGGGAACTGGAGTCTTACTGAGACCATGGTTCTGATCGAGGCGAA 480
************************************************************
BB_CT-35.1 GAAGATGGAT-ACGAGAGAAGGGCGCAGAGGTTGAAGGAGTTGCCGCGGGGCAGCAGCGG 539
chr4 GAAGATGGATTACGAGAGAAGGGCGCAGAGGTTGAAGGAGTTGCCGCGGGGCAGCAGCGG 540
********** *************************************************
BB_CT-35.1 CATCGGCAGTAGCAGGCCACAAGAGATGAGGTGGAAGTGGGTGGAGGACTGTTGCTGGCG 599
chr4 CATCGGCAGTAGCAGGCCACAAGAGATGAGGTGGAAGTGGGTGGAGGACTGTTGCTGGCG 600
************************************************************
BB_CT-35.1 ACATGGGTGCTACAGGAGCCAAAACCAGTGCAATGACCGGTGGGATAATCTCATGAGGGA 659
chr4 ACATGGGTGCTACAGGAGCCAAAACCAGTGCAATGACCGGTGGGATAATCTCATGAGGGA 660
************************************************************
BB_CT-35.1 TTACAAGAAGGTCAGAACATATGAGCTGAGCTTGAGCGCCGGAGGAAGCCCAGGTTTATC 719
chr4 TTACAAGAAGGTCAGAACATATGAGCTGAGCTTGAGCGCCGGAGGAAGCCCAGGTTTATC 720
************************************************************
BB_CT-35.1 TTACTGGAAGCTCGAGAGGCATGAGAGGAAGGAGAAGAACCTGCCCTCCAATCTCTTGCC 779
chr4 TTACTGGAAGCTCGAGAGGCATGAGAGGAAGGAGAAGAACCTGCCCTCCAATCTCTTGCC 780
************************************************************
BB_CT-35.1 TGAGATATACGAAGCC 795
chr4 TGAGATATACGAAGCC 796
****************
Banana SSR markers – Primers Set 4
PET / BB_AAC-3
F: AAATTCGGGGGTCAAAAAGT 5’to3’
R: GAGGGATTTATGGGACGACA 5’to3’
SSR-enriched library clone sequence
Accession KM190162
>BB_AAC-3
TACGAGATATAAGGACCGCTACTACAAGCAGTACTACACCTTTGATTACACCTTTGATCATGAAATATCGATTGCTTGTTGAACCCTGCGAATCACGTGAAAGTAGGGTACTCCAAATTCGGGGGTCAAAAAGTTTCATAAAGCGTTCTTGGTGGAAAAAAATGTATTTGATCCACGAATCTAAGAAATAGTGAGAATTCTTGATCTCTCTCAATATCTCTCTCAATTCGACGATCCAGAATTTGAATTGATGTCGTCCCATAAATCCCTCCTAAAGATTTTATTTCAACTAAAGATTTTATTTCAATTGGAATTAGGTTATTTATACATTTACGATATACTTATTTATACGATATACTTATTTACGATATACTTATTTATACGATATACTTATTTATACATTTACGATATACTTATTTATACGATATACTTATTTATACGATATACTTATTTACGATATACTTATTTATACGATATACTTATTTACGATATACTTATTTACGATATACTTATTTATACATTTACGATATACTTATTTATACGATATACTTATTTATACGATATACTTATTTACGATATACTTATTTATACGATATACTTATTTACGATATACTTATTTACGATATACTTATTTACGATATACTTATTTATACGATATACTTATTTACGATATTATTTACGATATACGATGATCTCTG
Musa balbisiana ‘Pisung Klutuk Wulung’ sequence at Banana Genome Hub
>chrUn_random:2274771..2275469
TCTCTTATTTTGATGGAGCAGACTTATCTTACATGATGCCAAAGTCAAAAGGAGCTTCGAGACACATGCACCTTATCTCGATGAAGCATTTGACGGAGGCACTAAGACAACTCCAAATTCGGGGGTCAAAAAGTTTCATAAAGCGTTCTTGATGGAAAAAAATGTATTTGATCCACGAATCTAAGAAATAGTGAGAATTCTTGATCTCTCTCAATATCTCTCTCAATTCGACGATCCAGAATTTGAATTGATGTCGTCCCATAAATCCCTCCTAAAGATTTTATTTCAACTAAAGATTTTATTTCAATTGGAATTTGGTTATTTATACATTTACGATATACTTATTTATACGATATACTTATTTATACGATAACTTATAGAAGCGAAGTTGGGGTCAGAAAGCCTTAGCATAGGACAAGAGGATGTGGAGACGAGTACTTATGAATAATATATCATAGTATTACCATTCGAATTACTATAAAGGAAATGGTATGTAGCAAAGATTGTGTTGGGGCAAGGGCCTATGATCCAAACAATGACACACTAATTTCAATGATGTCAGTGGACTTCGAGAGCTATTAGGTGATGAACTATCCTAGAGTTGTGCTTCATCCATGTGTGATCTAAGAGTGGGTGGATGAAGGTCAATTGTTAAAAGAGCAAACACAATTGGAGATGGCAAAAGCCCTATGATGTGTT
CLUSTAL 2.1 multiple sequence alignment
BB_AAC-3 TACGAGATATAAGGACCGCTACTACAAGCAGTACTACACCTTTGATTACACCTTTGATCA 60
chrUn_random TCTCTTATTTTGATGGAGCAGACTTATCTTACATGATGCCAAAGTCAAAAGGAGCTTCGA 60
* ** * ** * * * ** * * * *
BB_AAC-3 TGAAATATCGATTGCTTGTTGAACCCTGCGAATCACGTGAAAGTAGGGTACTCCAAATTC 120
chrUn_random GACACATGCACCTTATCTCGATGAAGCATTTGACGGAGGCACTAAGACAACTCCAAATTC 120
* * * * * * * ** ***********
BB_AAC-3 GGGGGTCAAAAAGTTTCATAAAGCGTTCTTGGTGGAAAAAAATGTATTTGATCCACGAAT 180
chrUn_random GGGGGTCAAAAAGTTTCATAAAGCGTTCTTGATGGAAAAAAATGTATTTGATCCACGAAT 180
******************************* ****************************
BB_AAC-3 CTAAGAAATAGTGAGAATTCTTGATCTCTCTCAATATCTCTCTCAATTCGACGATCCAGA 240
chrUn_random CTAAGAAATAGTGAGAATTCTTGATCTCTCTCAATATCTCTCTCAATTCGACGATCCAGA 240
************************************************************
BB_AAC-3 ATTTGAATTGATGTCGTCCCATAAATCCCTCCTAAAGATTTTATTTCAACTAAAGATTTT 300
chrUn_random ATTTGAATTGATGTCGTCCCATAAATCCCTCCTAAAGATTTTATTTCAACTAAAGATTTT 300
************************************************************
BB_AAC-3 ATTTCAATTGGAATTAGGTTATTTATACATTTACGATATACTTATTTATACGATATACTT 360
chrUn_random ATTTCAATTGGAATTTGGTTATTTATACATTTACGATATACTTATTTATACGATATACTT 360
*************** ********************************************
BB_AAC-3 ATTTACGATATACTTATTTATACGATATACTTATTTATACATTTACGATATACTTATTTA 420
chrUn_random ATTTATACGATAACTTATAGAAGCGAAGTTGGGGTCAGAAAGCCTTAGCATAGGACAAGA 420
***** *** * * * * * * * * *** *
BB_AAC-3 TACGATATACTTATTTATACGATATACTTATTTACGATATACTTATTTATACGATATACT 480
chrUn_random GGATGTGGAGACGAGTACTTATGAATAATATATCATAGTATTACCATTCGAATTACTATA 480
* * ** * *** * * ** * **
BB_AAC-3 TATTTACGATATACTTATTTACGATATACTTATTTATACATTTACGATATACTTATTTAT 540
chrUn_random AAGGAAATGGTATGTAGCAAAGATTGTGTTGGGGCAAGGGCCTATGATCCAAACAATGAC 540
* * * * * * * * ** *** * * * *
BB_AAC-3 ACGATATACTTATTTATA-CGATATACTTATTTACGATATACTTATTTATACGATATACT 599
chrUn_random ACACTAATTTCAATGATGTCAGTGGACTTCGAGAGCTATTAGGTGATGAACTATCCTAGA 600
** ** * * * ** * * **** * ** * * * **
BB_AAC-3 TATTTACGATATACTTATTTACGATATACTTATTTACGATATACTTATTTATACGATATA 659
chrUn_random GTTGTGCTTCATCCATGTGTGATCTAAGAGTGGGTGGATGAAGGTCAATTGTTAAAAGAG 660
* * * ** * * * * ** * * * * * ** * *
BB_AAC-3 CTTATTTACGATATTATTTACGATATACGATGATCTCTG 698
chrUn_random CAAACACAATTGGAGATGGCAAAAGCCCTATGATGTGTT 699
* * * ** * * ***** * *
Banana SSR markers – Primers Set 5
FAM / BB_CT-8
F: GTTCAAGCATCCTCAGCACA 5’to3’
R: CCGAAAGGAGAAACCAGTTG 5’to3’
SSR-enriched library clone sequence
Accession KM190163
>BB_CT-8
CCCTAACACACTTTGGCTAGCTTGTTCTTTGCATTATTTTCCATGCTCTTCCTCTTCATGAGTTTCTTCACTTCAGATTCCCAGCACGTGTGCAAGTTCTTAGTCTGCTTTCTTCTTTTGCTTGCTTAGTGCAACAGCAATGATGGTGTGGCTCTCAAGAAGGCTAGAGTTCAAGCATCCTCAGCACAGTCTCTTCTCAAGGTTCTCATTTGTGTGATCATCATAATTGCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTAAAAAGGGAAATCTTATTCTATCTGATACATCAGGAGAAAGTATATATACTGGTTTATCTGTATTGGCTTCAGGTTAGGAAGGAGAAGTTGGGAGATAGGATATCAGCTCTTCATCAACTGGTTTCTCCTTTCGGAAAGGTAGTACCATATTACATTCTCCTTAGATCAAGATGCATCATCACTACCTGGTTGTTCGTGTTCTTTCTGCTGCTTCAACTTCCCTTTCCATGACA
Musa balbisiana ‘Pisung Klutuk Wulung’ sequence at Banana Genome Hub
>chr4:806924..807414
CCCTAACACACTTTGGCTAGCTTGATCTTTGCATTATTTTCCATGCTCTTCCTCTTCATGAGTTTCTTCACTTCAGATTCCCAGCACGTGTGCAAGTTCTTAGTCTGCTTTCTTCTTTTGCTTGCTTAGTGCAACAGCAATGATGGTGTGGCTCTCAAGAAGGCTAGAGTTCAAGCATCCTCAGCACAGTCTCTTCTCAAGGTTCTCATTTGTGTGATCATCATAATTGCTCTCTCTCTCTCTCTCTCTCTCTCTCTAAAAAGGGAAATCTTATTGTATCTGATACATCAGGAGAAAGTATTTATACTGGTTTATCTGTATTGGCTTCAGGTTAGGAAGGAGAAGTTGGGAGATAGGATATCAGCTCTTCATCAACTGGTTTCTCCTTTCGGAAAGGTAGTACCATATTACAGTCTCCTTAGATCAAGATGCATCATCACTACCTGGTTGTTCGTGTTCTTTCTGCTGCTTCAACTTCCCTTTCCATGACA
CLUSTAL 2.1 multiple sequence alignment
BB_CT-8 CCCTAACACACTTTGGCTAGCTTGTTCTTTGCATTATTTTCCATGCTCTTCCTCTTCATG 60
chr4 CCCTAACACACTTTGGCTAGCTTGATCTTTGCATTATTTTCCATGCTCTTCCTCTTCATG 60
************************ ***********************************
BB_CT-8 AGTTTCTTCACTTCAGATTCCCAGCACGTGTGCAAGTTCTTAGTCTGCTTTCTTCTTTTG 120
chr4 AGTTTCTTCACTTCAGATTCCCAGCACGTGTGCAAGTTCTTAGTCTGCTTTCTTCTTTTG 120
************************************************************
BB_CT-8 CTTGCTTAGTGCAACAGCAATGATGGTGTGGCTCTCAAGAAGGCTAGAGTTCAAGCATCC 180
chr4 CTTGCTTAGTGCAACAGCAATGATGGTGTGGCTCTCAAGAAGGCTAGAGTTCAAGCATCC 180
************************************************************
BB_CT-8 TCAGCACAGTCTCTTCTCAAGGTTCTCATTTGTGTGATCATCATAATTGCTCTCTCTCTC 240
chr4 TCAGCACAGTCTCTTCTCAAGGTTCTCATTTGTGTGATCATCATAATTGCTCTCTCTCTC 240
************************************************************
BB_CT-8 TCTCTCTCTCTCTCTCTCTCTCTCTAAAAAGGGAAATCTTATTCTATCTGATACATCAGG 300
chr4 TCTCTCTCTCTCTCTCT------AAAAAGGGAAATCTTATTGTATCTGATACATCAGG 292
***************** ****************** ****************
BB_CT-8 AGAAAGTATATATACTGGTTTATCTGTATTGGCTTCAGGTTAGGAAGGAGAAGTTGGGAG 360
chr4 AGAAAGTATTTATACTGGTTTATCTGTATTGGCTTCAGGTTAGGAAGGAGAAGTTGGGAG 352
********* **************************************************
BB_CT-8 ATAGGATATCAGCTCTTCATCAACTGGTTTCTCCTTTCGGAAAGGTAGTACCATATTACA 420
chr4 ATAGGATATCAGCTCTTCATCAACTGGTTTCTCCTTTCGGAAAGGTAGTACCATATTACA 412
************************************************************
BB_CT-8 TTCTCCTTAGATCAAGATGCATCATCACTACCTGGTTGTTCGTGTTCTTTCTGCTGCTTC 480
chr4 GTCTCCTTAGATCAAGATGCATCATCACTACCTGGTTGTTCGTGTTCTTTCTGCTGCTTC 472
***********************************************************
BB_CT-8 AACTTCCCTTTCCATGACA 499
chr4 AACTTCCCTTTCCATGACA 491
*******************
Banana SSR markers – Primers Set 5
HEX / BB_CT-33
F: GGCAATGTCTCATAAGAAAGAGAG 5’to3’
R: TTTTGCACCTTTGCAGAGAA 5’to3’
SSR-enriched library clone sequence
Accession KM190164
>BB_CT-33
ACAAGATGACAAATATCTCATTGTATATTTGAGGCAATGTCTCATAAGAAAGAGAGAGGGAGGAGTGTTGTGTGTGTGTGTGTGAGAGAGAGAGAGAGAGAGAGGGAGAGGGAGAATATAAACAAGATACCAGATATCATGTTCTAGGATTGAAGTGTAATAGGCAAAATACAGGATACTCTTGACTAGGTGGCTAGATGCACTTCTCTGCAAAGGTGCAAAAATTTAGTTCATCGCCGAACTTGTCATTCAGAAAAAGATGCTAAAAGTACTATTCTATTTATAGAGAAAAGATGAATGTAGATGGAAGTGCAATCTTCATCTGGACATTCACCATGGACAACAGCACAACTCTCACTTATGTACAGAT
Musa balbisiana ‘Pisung Klutuk Wulung’ sequence at Banana Genome Hub
>chr4:8894011..8894412
ATCTGTACATAAGTGAGAGTTGTGCTGTTGTCCATGGTGAATGTCCAGATGAAGATTGCACTTCCATCTACATTCATCTTTTCTTTATAAATAGAATAGTACTTTTAGCATCTTTTTCTGAATGACAAGTTCGGTGATGAACTAAATTTTTGCACCTTTGCAGAGAAGTGCATCTAGCCACTTAGTCAAGAGTATCCTGTATTTTGCTTATTACACTTCAATCCTAGAACATGATATCTGGTATCTTGTTTATATTCTCCCTCCCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCACACACACACACACACACACACACAACACTCCTCCCTCTCTCTTTCTTATGAGACATTGCCTCAAATATACAATGAGATATTTGTCATCTTGT
CLUSTAL 2.1 multiple sequence alignment
Note: Sequence of BB_CT-33 clone is reverse complemented
BB_CT-33 ATCTGTACATAAGTGAGAGTTGTGCTGTTGTCCATGGTGAATGTCCAGATGAAGATTGCA 60
chr4 ATCTGTACATAAGTGAGAGTTGTGCTGTTGTCCATGGTGAATGTCCAGATGAAGATTGCA 60
************************************************************
BB_CT-33 CTTCCATCTACATTCATCTTTTCTCTATAAATAGAATAGTACTTTTAGCATCTTTTTCTG 120
chr4 CTTCCATCTACATTCATCTTTTCTTTATAAATAGAATAGTACTTTTAGCATCTTTTTCTG 120
************************ ***********************************
BB_CT-33 AATGACAAGTTCGGCGATGAACTAAATTTTTGCACCTTTGCAGAGAAGTGCATCTAGCCA 180
chr4 AATGACAAGTTCGGTGATGAACTAAATTTTTGCACCTTTGCAGAGAAGTGCATCTAGCCA 180
************** *********************************************
BB_CT-33 CCTAGTCAAGAGTATCCTGTATTTTGCCTATTACACTTCAATCCTAGAACATGATATCTG 240
chr4 CTTAGTCAAGAGTATCCTGTATTTTGCTTATTACACTTCAATCCTAGAACATGATATCTG 240
* ************************* ********************************
BB_CT-33 GTATCTTGTTTATATTCTCCCTCTCCCTCTCTCTCTCTCTCTCTCTC------287
chr4 GTATCTTGTTTATATTCTCCCTCCCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT 300
*********************** * *********************
BB_CT-33 ------ACACACACACACACAACACTCCTCCCTCTCTCTTTCTTATG 328
chr4 CTCTCTCTCACACACACACACACACACACACACAACACTCCTCCCTCTCTCTTTCTTATG 360
*****************************************
BB_CT-33 AGACATTGCCTCAAATATACAATGAGATATTTGTCATCTTGT 370
chr4 AGACATTGCCTCAAATATACAATGAGATATTTGTCATCTTGT 402
******************************************
Banana SSR markers – Primers Set 5
NED / BB_GAA-32
F: TGCCACCACAATATATCACCA 5’to3’
R: CCAGTTGGTGTCCTTGTCCT 5’to3’
SSR-enriched library clone sequence
Accession KM190165
>BB_GAA-32
TCCTGCCACCACAATATATCACCATTCTTCTCTGTATTTTTTCTTCTTCTTCTTCTTCTTCTTCTTTGTATTGCTTTCCTCCAATTCTTCTTGTGTTTGTTCTGAGAGTCTTCTATTGTAATCTTCTTTCGCTGGTTTATGTTGCGAGCAGCGTTGCAGAATGATTATATTTCCTTTGTGGTACAGGCAGGACAAGGACACCAACTGGGAAGGGATCCACTCCAATCATATATAAAAACTAAAAGAAGAAGAAAGCTTTGCCGTCCTAATT
Musa balbisiana ‘Pisung Klutuk Wulung’ sequence at Banana Genome Hub
Database: PKW_pseudochromosome.fa
12 sequences; 402,547,155 total letters
Searching...... done
***** No hits found ******
Database: PKW_pseudochromosome.fa
Posted date: Dec 20, 2013 4:25 PM
Number of letters in database: 402,547,155
Number of sequences in database: 12
Banana SSR markers – Primers Set 5
PET / BB_GT-10
F: GAGTGATCCCACCTTGAGGA 5’to3’
R: AGCCAACCATCATTGGAGAC 5’to3’
SSR-enriched library clone sequence
Accession KM190166
>BB_GT-10
GCATCTTCCAACATGTGCTATCATCTCTTCCTGTACACTACACACACCCACACACAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAAGGAATAATTTTAGCAAAGAGAGTATGAGTTTGAGAGATGTGAGTGATCCCACCTTGAGGAAAGGGAGACAGGATGGTTCTGTTGGAGGTGTTTTGAATGGTTCCCAATCCCCAAAGAACAGTGAGAGAGAGAGGAGACAAGCCCATCAGCAGCAAAAGATTCTGAGAAGAGTCTCCAATGATGGTTGGCTCTTTGAAGACTTCAAGAACCTAGAAAATGAGACCTACATCACACACAGACACACACACTTAGACCTGTGTTTGCCATGATCTTCCATGCTAAGCCCACCACTTGTGTGATTTCATTGGCATCCCACCTACTCTTCCAAGAAATTGAATGCTGCAGCCCTAAAGGACAAAGACCATTGACCTGTGTTTGCTATAATCTTGTTCAGAGCAGTCCTGTCTAAAAGCTCCACTTCTCTGGTCTTGTCTTTTACCTCACAGCTCTACACCAGGAAACCTGTAAAACAGCAGCAGCTTATTCTTTGATGTTCTCCACTACAACCTCCAGACATGTAACCTCCAGACATGTCTAAAAGCTCCTTTTTGATCATTGGTCCCCTTTCTGATACA
Musa balbisiana ‘Pisung Klutuk Wulung’ sequence at Banana Genome Hub
>chr11:4286419..4287061
GCATCTTCCAACATGTGCTATCATCTCTTCCTGTACACTACAGATAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAAGGAATAATTTTAGCAAAGAGAGTATGAGTTTGAGAGATGCGAGTGATCCCACCTTGAGGAAAGGGAGACAGGATGGTTCTGTTGGAGGTGTTTTGAATGGTTCCCAATCCCCAAAGCACAGTGAGAGAGAGAGGAGACAAGCCCATCAGCAGCAAAAGATTCTGAGAAGAGTCTCCGATGATGGTTGGCTCTTTGAAGACTTCAAGAACCTAGAAAATGAGACCTACATCACACACACATCACACACTTAGACCTGTGTTTGCCATGATCTTCCATGCTAAGCCCACCACATGTGTGATTTCATTGGCATCACACCTACTCTTCCAAGAAATTGAATGCTGCAGCCCCAAAGGACAAAGACCATTGACCTGTGTTTGCCATAATCTTGTTTCAGAGCAGTCCTGTCTAAAAGCTCCACTTCTCTGGTCTTGTCTTTTACCTCACAGCTCTTCACCAGGAAACCTGTAAAACAGCAGCAGCTTATTCTTTGATGTTCTCCACTACAACCTCCAGACATGTCTAAAAGCTCCTTTTTGATCATTGGTCCCTTTCTGATACAT
CLUSTAL 2.1 multiple sequence alignment
BB_GT-10 GCATCTTCCAACATGTGCTATCATCTCTTCCTGTACACTACACACACCCACACACAGAGA 60
chr11 GCATCTTCCAACATGTGCTATCATCTCTTCCTGTACACTACAG------ATAGAGAGA 52
****************************************** * * *****
BB_GT-10 GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAAGGAATAATTTTAGCAAAGAGAGTATGAGT 120
chr11 GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAAGGAATAATTTTAGCAAAGAGAGTATGAGT 112
************************************************************
BB_GT-10 TTGAGAGATGTGAGTGATCCCACCTTGAGGAAAGGGAGACAGGATGGTTCTGTTGGAGGT 180
chr11 TTGAGAGATGCGAGTGATCCCACCTTGAGGAAAGGGAGACAGGATGGTTCTGTTGGAGGT 172
********** *************************************************
BB_GT-10 GTTTTGAATGGTTCCCAATCCCCAAAGAACAGTGAGAGAGAGAGGAGACAAGCCCATCAG 240
chr11 GTTTTGAATGGTTCCCAATCCCCAAAGCACAGTGAGAGAGAGAGGAGACAAGCCCATCAG 232
*************************** ********************************
BB_GT-10 CAGCAAAAGATTCTGAGAAGAGTCTCCAATGATGGTTGGCTCTTTGAAGACTTCAAGAAC 300
chr11 CAGCAAAAGATTCTGAGAAGAGTCTCCGATGATGGTTGGCTCTTTGAAGACTTCAAGAAC 292
*************************** ********************************
BB_GT-10 CTAGAAAATGAGACCTACATCACACACAGACACACACACTTAGACCTGTGTTTGCCATGA 360
chr11 CTAGAAAATGAGACCTACATCACACACACAT-CACACACTTAGACCTGTGTTTGCCATGA 351
**************************** * ****************************
BB_GT-10 TCTTCCATGCTAAGCCCACCACTTGTGTGATTTCATTGGCATCCCACCTACTCTTCCAAG 420
chr11 TCTTCCATGCTAAGCCCACCACATGTGTGATTTCATTGGCATCACACCTACTCTTCCAAG 411
********************** ******************** ****************
BB_GT-10 AAATTGAATGCTGCAGCCCTAAAGGACAAAGACCATTGACCTGTGTTTGCTATAATCTTG 480
chr11 AAATTGAATGCTGCAGCCCCAAAGGACAAAGACCATTGACCTGTGTTTGCCATAATCTTG 471
******************* ****************************** *********
BB_GT-10 TT-CAGAGCAGTCCTGTCTAAAAGCTCCACTTCTCTGGTCTTGTCTTTTACCTCACAGCT 539
chr11 TTTCAGAGCAGTCCTGTCTAAAAGCTCCACTTCTCTGGTCTTGTCTTTTACCTCACAGCT 531
** *********************************************************
BB_GT-10 CTACACCAGGAAACCTGTAAAACAGCAGCAGCTTATTCTTTGATGTTCTCCACTACAACC 599
chr11 CTTCACCAGGAAACCTGTAAAACAGCAGCAGCTTATTCTTTGATGTTCTCCACTACAACC 591
** *********************************************************
BB_GT-10 TCCAGACATGTAACCTCCAGACATGTCTAAAAGCTCCTTTTTGATCATTGGTCCCCTTTC 659
chr11 TCCAGACATG------TCTAAAAGCTCCTTTTTGATCATTGGTCCCTTTCT 636
********** ****************************** **
BB_GT-10 TGATACA 666
chr11 GATACAT 643
Banana SSR markers – Primers Set 6
FAM / BB_CT-20
F: GGGAGATAAAGGGCCAACTT 5’to3’
R: GATACAACAGGATCCGAAGCA 5’to3’
SSR-enriched library clone sequence
Accession KM190167
>BB_CT-20
GGGAGATAAAGGGCCAACTTTAGAACACACAAGAGACTAGCACATATAGCAACTTCTAAATGTCGGTGTGTCCAGTTTAGAAAATTCTTTCTTGGCTCAATCAAATAAAGTTATCAAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGATAACATATAATTGAAGAATATATGATATGAGTCCGATAATTGTAGCAGTATGAAGAAAACATGTATGCTGGGGCATGCTTCGGATCCTGTTGTATCTATGAGAGGAAATGGCAAAATGCCACCAAAACTAACCAAGTCTTCTTTCTGTGATACTCCGAGACCTCTTTCACATATTTTATCTCGACCGGCGGATAAGTTGTTGGGCAGTGCAAAGGAGCAGAAATCATGGTG
Musa balbisiana ‘Pisung Klutuk Wulung’ sequence at Banana Genome Hub
>chr4:6032639..6033024
CACCATGATTTCTGCTCCTTTGCACTGCCCAACAACTTATCCGCCGGTCGAGATAAAATATGTGAAAGAGGTCTCGGAGTATCACAGAAAGAAGACTTGGTTAGTTTTGGTGGCATTTTGCCATTTCCTCTCATAGATACAACAGGATCCAAAGCATGCCCCAGCATACATGTTTTCTTCATACTGCTACAATTATCGGACTCATATCATATATTCTTCAATTATATGTTATCTCTCTCTCTCTCGATCGATCGATCGATCGATCGATCTTGATAACTTTATTTGATTGAGCCAAGAAAGAATTTTCTAAACTGGACACACCAACATTTAGAAGTTGCTATATGTGCTAGTCTCTTGTGTGTTCTAAAGTTGGCCCTTTATCTCCC
CLUSTAL 2.1 multiple sequence alignment
Note: Sequence of BB_CT-20 clone is reverse complemented
BB_CT-20 CACCATGATTTCTGCTCCTTTGCACTGCCCAACAACTTATCCGCCGGTCGAGATAAAATA 60
chr4 CACCATGATTTCTGCTCCTTTGCACTGCCCAACAACTTATCCGCCGGTCGAGATAAAATA 60
************************************************************
BB_CT-20 TGTGAAAGAGGTCTCGGAGTATCACAGAAAGAAGACTTGGTTAGTTTTGGTGGCATTTTG 120
chr4 TGTGAAAGAGGTCTCGGAGTATCACAGAAAGAAGACTTGGTTAGTTTTGGTGGCATTTTG 120
************************************************************
BB_CT-20 CCATTTCCTCTCATAGATACAACAGGATCCGAAGCATGCCCCAGCATACATGTTTTCTTC 180
chr4 CCATTTCCTCTCATAGATACAACAGGATCCAAAGCATGCCCCAGCATACATGTTTTCTTC 180
****************************** *****************************
BB_CT-20 ATACTGCTACAATTATCGGACTCATATCATATATTCTTCAATTATATGTTATCTCTCTCT 240
chr4 ATACTGCTACAATTATCGGACTCATATCATATATTCTTCAATTATATGTTATCTCTCTCT 240
************************************************************
BB_CT-20 CTCTCTCTCTCTCTCTCTCTCTCTC--TCTTGATAACTTTATTTGATTGAGCCAAGAAAG 298
chr4 CTCTCGATCGATCGATCGATCGATCGATCTTGATAACTTTATTTGATTGAGCCAAGAAAG 300
***** ** ** ** ** ** *********************************
BB_CT-20 AATTTTCTAAACTGGACACACCGACATTTAGAAGTTGCTATATGTGCTAGTCTCTTGTGT 358
chr4 AATTTTCTAAACTGGACACACCAACATTTAGAAGTTGCTATATGTGCTAGTCTCTTGTGT 360
********************** *************************************
BB_CT-20 GTTCTAAAGTTGGCCCTTTATCTCCC 384
chr4 GTTCTAAAGTTGGCCCTTTATCTCCC 386
**************************
Banana SSR markers – Primers Set 6
HEX / BB_GT-47
F: TGTGGTTCTTAGCCTTGCACT 5’to3’
R: CCAAATACTAGGGACTCCCACA 5’to3’
SSR-enriched library clone sequence
Accession KM190168
>BB_GT-47
TGCTATAACTCTGCTTACAGTTGATGCTTGGATGCCCATGACATATTTTGTGGTTCTTAGCCTTGCACTTGTAATTATTATGGTTTTGTTTATCATGTCATGTATCCTTAATTTTAAGGCCCATGTAAATCTTAGGAGGCTTGAGGTAGTAACTCTGAGGTAAAAGTGGAGATTTGTGTGTGTGATGCACGTGCTTTACCAGATAATTTTTTGTGGTTTTACTTTATTTATATGATTTAGACCTACTTGGAAAACATTATAAAGACTATGTGGGAGTCCCTAGTATTTGGGTAAAAAATATTTATCTATTGCTTATTTCTTGA
Musa balbisiana ‘Pisung Klutuk Wulung’ sequence at Banana Genome Hub
>chr6:5561281..5561578
TGCTATAACTCTCCTTACAGTTGATGCTTGGATGCCCATGACATATTTTGTGGTTCTTAGCCTTGCACTTGTAATTATTATGGTTTTGTTTATCATGTCATTTAGGCTTAAAGGCTTAAGGTAGTAACTCTGAGGTAAAAGTGGAGATTTGTGTGTGTGATGCACATGCTTTACTAGATAAGTTTTTGTGGTTTTACTTGATTTATATGATTTAGACCTATTTGGAAAACATTATAAAGACTATGTGGGAGTCCCTAGTATTTGGGTGAAAAATATTTATCTATTGCTTATTTCTTGA
CLUSTAL 2.1 multiple sequence alignment
BB_GT-47 TGCTATAACTCTGCTTACAGTTGATGCTTGGATGCCCATGACATATTTTGTGGTTCTTAG 60
chr6 TGCTATAACTCTCCTTACAGTTGATGCTTGGATGCCCATGACATATTTTGTGGTTCTTAG 60
************ ***********************************************
BB_GT-47 CCTTGCACTTGTAATTATTATGGTTTTGTTTATCATGTCATGTATCCTTAATTTTAAGGC 120
chr6 CCTTGCACTTGTAATTATTATGGTTTTGTTTATCATGTCATTTAGGCTTAA------111
***************************************** ** *****
BB_GT-47 CCATGTAAATCTTAGGAGGCTTGAGGTAGTAACTCTGAGGTAAAAGTGGAGATTTGTGTG 180
chr6 ------AGGCTTAAGGTAGTAACTCTGAGGTAAAAGTGGAGATTTGTGTG 155
****** *************************************
BB_GT-47 TGTGATGCACGTGCTTTACCAGATAATTTTTTGTGGTTTTACTTTATTTATATGATTTAG 240
chr6 TGTGATGCACATGCTTTACTAGATAAGTTTTTGTGGTTTTACTTGATTTATATGATTTAG 215
********** ******** ****** ***************** ***************
BB_GT-47 ACCTACTTGGAAAACATTATAAAGACTATGTGGGAGTCCCTAGTATTTGGGTAAAAAATA 300
chr6 ACCTATTTGGAAAACATTATAAAGACTATGTGGGAGTCCCTAGTATTTGGGTGAAAAATA 275
***** ********************************************** *******
BB_GT-47 TTTATCTATTGCTTATTTCTTGA 323
chr6 TTTATCTATTGCTTATTTCTTGA 298
***********************
Banana SSR markers – Primers Set 6
NED / BB_GAA-4
F: GATTGCTTGCAGATAATGAACTTT 5’to3’
R: GTACCGAAGCTTCCACCAAA 5’to3’
SSR-enriched library clone sequence
Accession KM190169
>BB_GAA-4
GATTGCTTGCAGATAATGAACTTTCTAGAGCTGGCCTGGCCACAAAGGAAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAATGCACACACAGGCACCACACATGAAAAGGAAGACAGACGTGGAGAGTTATCAGCAGAAACAAGATGAAATATCTTTGGTGGAAGCTTCGGTACCCACAAAAATTGAAGATCCACCTGTAAGCAGGTGAGGGAA
Musa balbisiana ‘Pisung Klutuk Wulung’ sequence at Banana Genome Hub
>chr9:4035113..4035315
GATTGCTTGCAGATAATGAACTTTCTAGAGCTGGCCACAAAGAAAGAGAGAGAGAGAGAGAGAGAGAGAATGCACACACAGGTACCACACATGAAAAGGAACAGAGACGTGGAGAGTTATCAGCAGAAACAAGATGAAATATCTTTGGTGGAAGCTTCGGTATCCACAAAAATTGAAGATCCACCTGTAAGCAGGTGAGGGCA
CLUSTAL 2.1 multiple sequence alignment
BB_GAA-4 GATTGCTTGCAGATAATGAACTTTCTAGAGCTGGCCTGGCCACAAAGGAAGAGAGAGAGA 60
chr9 GATTGCTTGCAGATAATGAACTTTCTAGAGCTGGCC-----ACAAAGAAAGAGAGAGAGA 55
************************************ ****** ************
BB_GAA-4 GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAATGCACACACAGGCACCACACATGAA 120
chr9 GAGAG------AGAGAGAGAATGCACACACAGGTACCACACATGAA 95
***** ********************** ************
BB_GAA-4 AAGGAAGACAGACGTGGAGAGTTATCAGCAGAAACAAGATGAAATATCTTTGGTGGAAGC 180
chr9 AAGGAACAGAGACGTGGAGAGTTATCAGCAGAAACAAGATGAAATATCTTTGGTGGAAGC 155
****** * ***************************************************
BB_GAA-4 TTCGGTACCCACAAAAATTGAAGATCCACCTGTAAGCAGGTGAGGGAA 228
chr9 TTCGGTATCCACAAAAATTGAAGATCCACCTGTAAGCAGGTGAGGGCA 203
******* ************************************** *
Banana SSR markers – Primers Set 6
PET / BB_GT-37
F: CAACATCACGTTGCCCATAA 5’to3’
R: ACATATGTTGGGGCATTGCT 5’to3’
SSR-enriched library clone sequence
Accession KM190170
>BB_GT-37
TGGGTGGCTAATGCATTCTCATCCTTTCTGGTCTGATCTAACATGGAATTCATTTCATCCTCCTCCTACTGCCCTAAAGACTTCCTCCCCCCACATGCCCAAAAGGCCAACATCACGTTGCCCATAAATGTGCACCTATTGTCAAACCAACTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCACACACACACACACACACACACACACACAAACACTAAATCTATCTCCTCCTCTTGCTTGTAGGAGTCCCAACTCTCAAGCAATGCCCCAACATATGTTTTTTCTTTTTTCACATCAACAGATCAACTCTACCTTTTTCAAAACCTTCATGTTTTTTTATTGCGCAGAAGCTTCTCATTTTCCTTCAACCTTGTATTATTTTATTCCCGCAAAGACCAACTGTTACAGAGAAGAGAGAGACAAAGCGAGAGAGGGAGAGAGAGTGTGCGTGTGTGGAAACAAATAGCTCAGTTGGTAGATCTGCTCTCTTGTTGTCTTCCTGCTCTCTTCTTCACCTCAAAACCATCGTATTCCCTCGGGGTGAGCCTCAACATCTCAAATCCATCAACCCACCCATTTGCAGGACTCTCTTTGCTGCTGTGAGTGCATAGAGAGTGTGTTGACATCCAGTGCTGGTTCCTGTGCGGTTGGTGCAGGTGACCACCCTGCATTCCGCGGCCTAAGGATGAAGAATTGAAGCAGGGGTCGAAGATGTTCAAGGCGGCGCGATGGAGGAGCGAGAAGAACAGGAGCAAGGCCGTCTTCAAGCTGCAGTTCCATGCGACGCAGGTCGGTTTGGTGTCACATGTCTGTCAGCTCACCTTACTCTCTCACTCCGACCGACAAATGTTACAGAGATCTGTAGATAGATTATGCCTTTGGGGTGGTTGAATTCTGGTAGGTGCCGCTGTTAGCGTCGGAGGCGATGATGGTCAGCCTGGTCCCGGCGGACGTCGCGAAGCCGA
Musa balbisiana ‘Pisung Klutuk Wulung’ sequence at Banana Genome Hub
>chr1:2115276..2116207
TGGGTGGCTAATGCATTCTCATCCTTTCTGGTCTGATCTAACATGGAATTCATTTCATCCTCCTCCTACTGCCCTAAAGACTTCCTCCCCCCACATGCCCAAAAGGCCAACATCACGTTGCCCATAAATGTGCACCTATTGTCAAACCAACTTCTCTCTCTCTCTCTCTCTCTCTCTCTCACTAAATCTATCTCCTCCTCTTGCTTGTAGGAGTCCCAACTCTCAAGCAATCCCCCAACATATGTTTTTTCTTTTTTCACATCAACAGATCCACTCTACCTTTTTCAAAACCTTCATGTTTTTTTATTGCGCAGAAGCTTCTCATTTTCCTTCAACTTTGTATTATTTTATTCCCGCAAAGACCAACTGTTACAGAGAAGAGAGAGACAAAGAGAGAGAGGGAGAGAGAGTGTGCGTGTGTGGAAACAAATAGCTCAGTTGGTAGATCTGCTCTCTTGTTGTCTTCCTGCTCTCTTCTTCACCTCAAAACCATCGTATTCCCTCGGGGTGAGCCTCAACATCTCAAATCCATCAACCCACCCATTTGCAGGACTCTCTTTGCTGCTGTGAGTGCATAGAGAGTGTGTTGACATCCAGTGCTGGTTCCTGTGCGGTTGGTGCAGGTGACCACCCTGCATTCCGCGGCCTAAGGATGAAGAATTGAAGCAGGGGTCGAAGATGTTCAAGGCGGCGCGATGGAGGAGCGAGAAGAACAGGAGCAAGGCCGTCTTCAAGCTGCAGTTCCATGCGACGCAGGTCGGTTTGGTGTCACATGTCTGTCAGCTCACCTTACTCTCTCACTCCGACCGACAAATGTTACAGAGATCTGTAGATAGATTATGCCTTTGGGGTGGTTGAATTCTGGTAGGTGCCGCTGTTAGCGTCGGAGGCGATGATGGTCAGCCTGGTCCCGGCGGACGTCGCCAAGCCGA
CLUSTAL 2.1 multiple sequence alignment
BB_GT-37 TGGGTGGCTAATGCATTCTCATCCTTTCTGGTCTGATCTAACATGGAATTCATTTCATCC 60
chr1 TGGGTGGCTAATGCATTCTCATCCTTTCTGGTCTGATCTAACATGGAATTCATTTCATCC 60
************************************************************
BB_GT-37 TCCTCCTACTGCCCTAAAGACTTCCTCCCCCCACATGCCCAAAAGGCCAACATCACGTTG 120
chr1 TCCTCCTACTGCCCTAAAGACTTCCTCCCCCCACATGCCCAAAAGGCCAACATCACGTTG 120
************************************************************
BB_GT-37 CCCATAAATGTGCACCTATTGTCAAACCAACTTCTCTCTCTCTCTCTCTCTCTCTCTCTC 180
chr1 CCCATAAATGTGCACCTATTGTCAAACCAACTTCTCTCTCTCTCTCTCTCTCTCTCTCTC 180
************************************************************
BB_GT-37 TCTCTCACACACACACACACACACACACACACACAAACACTAAATCTATCTCCTCCTCTT 240
chr1 ------ACTAAATCTATCTCCTCCTCTT 202
**********************
BB_GT-37 GCTTGTAGGAGTCCCAACTCTCAAGCAATGCCCCAACATATGTTTTTTCTTTTTTCACAT 300
chr1 GCTTGTAGGAGTCCCAACTCTCAAGCAATCCCCCAACATATGTTTTTTCTTTTTTCACAT 262
***************************** ******************************
BB_GT-37 CAACAGATCAACTCTACCTTTTTCAAAACCTTCATGTTTTTTTATTGCGCAGAAGCTTCT 360
chr1 CAACAGATCCACTCTACCTTTTTCAAAACCTTCATGTTTTTTTATTGCGCAGAAGCTTCT 322
********* **************************************************
BB_GT-37 CATTTTCCTTCAACCTTGTATTATTTTATTCCCGCAAAGACCAACTGTTACAGAGAAGAG 420
chr1 CATTTTCCTTCAACTTTGTATTATTTTATTCCCGCAAAGACCAACTGTTACAGAGAAGAG 382
************** *********************************************
BB_GT-37 AGAGACAAAGCGAGAGAGGGAGAGAGAGTGTGCGTGTGTGGAAACAAATAGCTCAGTTGG 480
chr1 AGAGACAAAGAGAGAGAGGGAGAGAGAGTGTGCGTGTGTGGAAACAAATAGCTCAGTTGG 442
********** *************************************************
BB_GT-37 TAGATCTGCTCTCTTGTTGTCTTCCTGCTCTCTTCTTCACCTCAAAACCATCGTATTCCC 540
chr1 TAGATCTGCTCTCTTGTTGTCTTCCTGCTCTCTTCTTCACCTCAAAACCATCGTATTCCC 502
************************************************************
BB_GT-37 TCGGGGTGAGCCTCAACATCTCAAATCCATCAACCCACCCATTTGCAGGACTCTCTTTGC 600
chr1 TCGGGGTGAGCCTCAACATCTCAAATCCATCAACCCACCCATTTGCAGGACTCTCTTTGC 562
************************************************************
BB_GT-37 TGCTGTGAGTGCATAGAGAGTGTGTTGACATCCAGTGCTGGTTCCTGTGCGGTTGGTGCA 660
chr1 TGCTGTGAGTGCATAGAGAGTGTGTTGACATCCAGTGCTGGTTCCTGTGCGGTTGGTGCA 622
************************************************************
BB_GT-37 GGTGACCACCCTGCATTCCGCGGCCTAAGGATGAAGAATTGAAGCAGGGGTCGAAGATGT 720
chr1 GGTGACCACCCTGCATTCCGCGGCCTAAGGATGAAGAATTGAAGCAGGGGTCGAAGATGT 682
************************************************************
BB_GT-37 TCAAGGCGGCGCGATGGAGGAGCGAGAAGAACAGGAGCAAGGCCGTCTTCAAGCTGCAGT 780
chr1 TCAAGGCGGCGCGATGGAGGAGCGAGAAGAACAGGAGCAAGGCCGTCTTCAAGCTGCAGT 742
************************************************************
BB_GT-37 TCCATGCGACGCAGGTCGGTTTGGTGTCACATGTCTGTCAGCTCACCTTACTCTCTCACT 840
chr1 TCCATGCGACGCAGGTCGGTTTGGTGTCACATGTCTGTCAGCTCACCTTACTCTCTCACT 802
************************************************************
BB_GT-37 CCGACCGACAAATGTTACAGAGATCTGTAGATAGATTATGCCTTTGGGGTGGTTGAATTC 900
chr1 CCGACCGACAAATGTTACAGAGATCTGTAGATAGATTATGCCTTTGGGGTGGTTGAATTC 862
************************************************************
BB_GT-37 TGGTAGGTGCCGCTGTTAGCGTCGGAGGCGATGATGGTCAGCCTGGTCCCGGCGGACGTC 960
chr1 TGGTAGGTGCCGCTGTTAGCGTCGGAGGCGATGATGGTCAGCCTGGTCCCGGCGGACGTC 922
************************************************************
BB_GT-37 GCGAAGCCGA 970
chr1 GCCAAGCCGA 932
** *******
Banana SSR markers – Primers Set 7
FAM / BB_CT-38.2
F: TACTCCCCCACCATAAGAGC 5’to3’
R: CAAATCCTTTGCGTTGTTCC 5’to3’
SSR-enriched library clone sequence
Accession KM190171
>BB_CT-38.2
TGACCACCAAAGTCATGTAGCATTTAGAAGACTACTCCCCCACCATAAGAGCTCCTTTATCCAATCCTCCATGTCCTATCACACGCATGAATAGAGATATGGCCATATTATATTGCAGCAGTCACTAATTGAGTCTTTGGTGCATTTCTCCTCCTCCTCCACTAGGGGAGAAAGAGTGGGCGCTAAAAGACAGCTGGGTTTGGTGATTATGTTCCGATGCCCTATTAGTTTGCATGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAAGCAGACACGCAAAGGAACGGGAACAACGCAAAGGATTTGACTAGGGCACGGGGGGGTTTGATCACCCCGGGACCCTAAGTGCTATTCTTCCGAGTTG
Musa balbisiana ‘Pisung Klutuk Wulung’ sequence at Banana Genome Hub
>chr9:6707891..6708245
CAACTCGGAAGAATGGCATTTAGGGTCACGGGGTGATCAAACCACCCAGTGCCCTAGTCAATTCCTTTGCGTTGTTCCTGTTCCTTTGCGTGTCTGCTTCTCTCTCTCTCTCTCTCTCTCTCATGCAAACTAATAGGGCATCGGAACATAATCACCAAACCCAGCTGTCTTTTAGCGCCCACTCTTTCTCCTCTAGTGGAGGAGGAGAAATGCAGCAACGACTCAATTAGTGACTGCTGCAATATAATATGGCCATGATCTCTATTCATGCGTGTGATAGGACATGGAGGATTGGATAAAGGAGCTCTTATGGTGGGGGAGTAGTCTTCTAAATGCTACATGACTTTGGTGGTCA
CLUSTAL 2.1 multiple sequence alignment
Note: Sequence of BB_CT-38 clone is reverse complemented
BB_CT-38.2 CAACTCGGAAGAATAGCACTTAGGGTCCCGGGGTGATCAAACCCCCCCGTGCCCTAGTCA 60
chr9 CAACTCGGAAGAATGGCATTTAGGGTCACGGGGTGATCAAACCACCCAGTGCCCTAGTCA 60
************** *** ******** *************** *** ************
BB_CT-38.2 AATCCTTTGCGTTGTTCCCGTTCCTTTGCGTGTCTGCTTCTCTCTCTCTCTCTCTCTCTC 120
chr9 ATTCCTTTGCGTTGTTCCTGTTCCTTTGCGTGTCTGCTTCTCTCTCTCTCTCTCT----- 115
* **************** ************************************
BB_CT-38.2 TCTCTCTCTCTCATGCAAACTAATAGGGCATCGGAACATAATCACCAAACCCAGCTGTCT 180
chr9 -----CTCTCTCATGCAAACTAATAGGGCATCGGAACATAATCACCAAACCCAGCTGTCT 170
*******************************************************
BB_CT-38.2 TTTAGCGCCCACTCTTTCTCCCCTAGTGGAGGAGGAGGAGAAATGCACCAAAGACTCAAT 240
chr9 TTTAGCGCCCACTCTTTCTCCTCTAGTGGAGGAGGAG---AAATGCAGCAACGACTCAAT 227
********************* *************** ******* *** ********
BB_CT-38.2 TAGTGACTGCTGCAATATAATATGGCCAT-ATCTCTATTCATGCGTGTGATAGGACATGG 299
chr9 TAGTGACTGCTGCAATATAATATGGCCATGATCTCTATTCATGCGTGTGATAGGACATGG 287
***************************** ******************************
BB_CT-38.2 AGGATTGGATAAAGGAGCTCTTATGGTGGGGGAGTAGTCTTCTAAATGCTACATGACTTT 359
chr9 AGGATTGGATAAAGGAGCTCTTATGGTGGGGGAGTAGTCTTCTAAATGCTACATGACTTT 347
************************************************************
BB_CT-38.2 GGTGGTCA 367
chr9 GGTGGTCA 355
********
Banana SSR markers – Primers Set 7
HEX / BB_GAA-35
F: GGGTTTTCACCCACCTCTTT 5’to3’
R: CGAAACCATTTCCCTCTGAT 5’to3’
SSR-enriched library clone sequence
Accession KM190172
>BB_GAA-35
GGGTTTTCACCCACCTCTTTCTGCAGCCTCTCCCTCGTACGAGAGAGGGGTTTCTGGTGAAACAGAGAGGAGAGGAGGAGGGAAGGAAGAGAAGAAGAAAAGAAGAAAGGGAGAAAGGAAGATAGAAGATAGGGAGAAGGCTTCGGCTGTGAGAACGGAGGAGAAGAAAGATAATGGTAAGAAAGAAAGAGAAGAAGAAGAAACAGATCAGAGGGAAATGGTTTCGGCGTGCAGAGAAGAAAGAATAGAGAGAAGAAGAAGAAAAATAAAGAAAAACTATATTGAGGACTTGATTTGGTAGCAAATGAATTGAGATCGATTTCATGATAATTATATTAGGTATGATTTTTATTTTTTTAGATTTTTATTTTTTTTATTTTTTTAT
Musa balbisiana ‘Pisung Klutuk Wulung’ sequence at Banana Genome Hub
>chr2:14121176..14121453
AATCTATAATTCAAAACATAAATCTAATCTAAAAAAATAAAAATCATACCTAATATAATTATCATGAAATTGATCTCAATTCATTTGATACCCTACCAAGTTCTCAATATAATTTTGCTAATTTTCTTTTTTTTTCTCTCTTATCTTTCTTCTCCTCCGTTCTCACAGCCGAAGCCTTCTCCCTTTCTTCTTTTCTTCTTCTCTTCCTTCCCTCCTCCTCTCCTCTCTGTTTCACCAGAAACCCCTCTCTCGTACGAGGGAGAGGTGGGTGAAAACCC
CLUSTAL 2.1 multiple sequence alignment
Note: Sequence of BB_GAA-35 clone is reverse complemented
BB_GAA-35 ATAAAAAAATAAAAAAAATAAA---AATCTAAAAAAATAAAAATCATACCTAATATAATT 57
chr2 AATCTATAATTCAAAACATAAATCTAATCTAAAAAAATAAAAATCATACCTAATATAATT 60
* * *** **** ***** ***********************************
BB_GAA-35 ATCATGAAATCGATCTCAATTCATTTGCTACCAAATCAAGTCCTCAATATAGTTTTTCTT 117
chr2 ATCATGAAATTGATCTCAATTCATTTGATACCCTACCAAGTTCTCAATATAATTTTGCTA 120
********** **************** **** * ***** ********* **** **
BB_GAA-35 TATTTTTCTTCTTCTTCTCTCTATTCTTTCTTCTCTGCACGCCGAAACCATTTCCCTCTG 177
chr2 -ATTTTCTTTTTTTTTCTCTCT------141
***** ** ** ********
BB_GAA-35 ATCTGTTTCTTCTTCTTCTCTTTCTTTCTTACCATTATCTTTCTTCTCCTCCGTTCTCAC 237
chr2 ------TATCTTTCTTCTCCTCCGTTCTCAC 166
*************************
BB_GAA-35 AGCCGAAGCCTTCTCCCTATCTTCTATCTTCCTTTCTCCCTTTCTTCTTTTCTTCTTCTC 297
chr2 AGCCGAAGCCTTCTCCCT------TTCTTCTTTTCTTCTTCTC 203
****************** *******************
BB_GAA-35 TTCCTTCCCTCCTCCTCTCCTCTCTGTTTCACCAGAAACCCCTCTCTCGTACGAGGGAGA 357
chr2 TTCCTTCCCTCCTCCTCTCCTCTCTGTTTCACCAGAAACCCCTCTCTCGTACGAGGGAGA 263
************************************************************
BB_GAA-35 GGCTGCAGAAAGAGGTGGGTGAAAACCC 385
chr2 GG------TGGGTGAAAACCC 278
** *************
Banana SSR markers – Primers Set 7
NED / BB_CT-36
F: GGAGCTTGTGATGTGGGAAT 5’to3’
R: CCAAGATGTTGATGCTGACC 5’to3’
SSR-enriched library clone sequence
Accession KM190173
>BB_CT-36
GGCAGGAACCAATTTTATTTTTATTCAATTGGAAAACCAGCAAGAACAATATCAAAAAGTCTAATCTGGGAGCTTGTGATGTGGGAATTTACAGTTCAAAATAAGGAAACCCAATTTTGACCGCAACAGTAGGCGGGGAGAGAGAGAGAGAGAGAGAGAGAGAAGAGACGTAGTAAGTTTCGATACACCACGACAAAATTCCAACCAAGCAAGCCCCAGAACACACGCGACCGATCCAGCGGTCAGCATCAACATCTTGGAAGATCGGACGGTGGAGGAGGGAGCTTCTGGTTGGGGAGTGGCCCGTCGTTGACCACCCACACCATCTTCAACCCCCAGCTGAGATGCAAGTCGATGTGGCAGTGCATGAGCCATGCACCTGTTCCGTACCCAAGTCAACGTCCAGCAACTAAAGAAACCACCAGTGAAGATAGTAGTATCTGTCACTTACCGGGGTTGTCAGCTCGGAAGCGAATGGCGAGCCATCCGCCCACCGGGACGCCGGCGGTGTTCTTCTCCACAGGGTCGACGAGATTGAAGTTACAAGGCTCGGTTACCGGGTCATAGTTTCCGAAGCCTTGTCCCACCACGTGGAAGTTGTGGCCATGGAAGTGCATCGGATGGTTCTCGATTCCTTGAATGCTGGTGTCCTGCAGCACCAACTCCACGCTGGTGTTGAACGGAAGCACCACCACCTTGGTTCCCTTGCTCACGAACGTGTTGTTGGGTGGAGTCCCCGTGTAGTTGAAGGGGAACGGAGGGATGTCCGGGAAGTCTGTGGTGTAGACCCCGTTCGACTGATTGTAGTAGTGAGCCTGGAGAAAAGCAGTGGGGGGGAACACAAAGGAGGCGTTGTTGACCGATGCAGCAAACTTGGTGCCATTGGGGCCTTGACAGGTCTGGTTCGTGGGACAGGGGTCCGTGCCGAGGCCGATCGTGAAGTAAAATCGCCGATCGACGGTTCGTGGTACGTCGGAGGGTACCGATCATCCGCGAGGCTGCAAAGCTTGCTGGTGAAGTTCGGGACGTTTGCAGTGTCGTACTCAGGACTCATCATTCTTTCTAAAGAATCCCCC
Musa balbisiana ‘Pisung Klutuk Wulung’ sequence at Banana Genome Hub
>chr9:2094599..2095660
AGCTGCCGCTGCTGAGACCGGCACTTCCGTCCCTCAACGACACTGCTAACGTCACGAACTTCACCAACAAGCTTCGCAGCCTCGCGGATGATCGGTACCCCTCCGACGTACCACGAACCGTCGACCAGCGATTCTACTTCACGGTCGGCCTCGGCACGGACCCCTGTCCCACGAACCGAACCTGTCAAGGCCCCAATGGCACCAAGTTTGCTGCATCGGTCAACAACGCCTCCTTTGTGTTTCCCTCCACTGCTCTTCTCCAGGCTCACTACTACTCGCAGTCGAACGGGGTCTACACCACAGACTTCCCGGACATCCCTCCGTTCCCCTTCAACTACACGGGGACTCCACCCAACAACACGTTCGTGAGCAAGGGAACCAAGGTGGTGGTGCTTCCGTTCAACACCAGCGTGGAGTTGGTGCTGCAGGACACCAGCATTCAAGGAATCGAGAACCACCCGATGCACCTCCATGGCCACAACTTCCACGTGGTGGGACAAGGCTTCGGAAACTATGACCCGGTGACCGATCCTTCTAACTTCAATCTCGTCGACCCCGTGGAGAAGAACACCGCCGGCGTCCCGGTCGGCGGATGGCTCGCCATTCGCTTCCGAGCTGACAACCCCGGTAAGTGATACTACTATCTTCAGTGGTGGTTTCTTTAGTTGCTGACGTTGACTTGGGTACGTAACAGGTGCATGGCTCATGCACTGCCACATCGACTTGCATCTCAGCTGGGGATTGAAGATGGTGTGGGTGGTCAACGACGGGCCACTCCCCAACCAGAAGCTCCCTCCTCCGCCGTCCGATCTTCCAAGATGTTGATTCTGACCGCTGGATCGGTCGCCTGTGTCCTGGGTCTTGCTGGTTGGTATTTTGTCGTGGTGTATCGAAACTTACTACGTATCTTCTCTCTCTCTCTCTCTCTCTCCCCGCCTACTGTTGCGGTCAAAATTGGGTTTCCTTATTTTGCACTGTAAATTTCCACATCACAAGCTCCCAAATTAGACTTTTTGATCTTGTTCTTGCTGGTTTTCCAATTGAATAAAATTGGTTCCTGCC
CLUSTAL 2.1 multiple sequence alignment
Note: Sequence of BB_CT-36 clone is reverse complemented
BB_CT-36 GGGGGATTCTTTAGAAA---GAATGATGAGTCCTGAGTACGACACTGCAAACGTCCCGAA 57
chr9 AGCTGCCGCTGCTGAGACCGGCACTTCCGTCCCTCA--ACGACACTGCTAACGTCACGAA 58
* * ** ** * * * *** * ********** ****** ****
BB_CT-36 CTTCACCAGCAAGCTTTGCAGCCTCGCGGATGATCGGTACCC-TCCGACGTACCACGAAC 116
chr9 CTTCACCAACAAGCTTCGCAGCCTCGCGGATGATCGGTACCCCTCCGACGTACCACGAAC 118
******** ******* ************************* *****************
BB_CT-36 CGTCGATCGGCGATTTTACTTCACGATCGGCCTCGGCACGGACCCCTGTCCCACGAACCA 176
chr9 CGTCGACCAGCGATTCTACTTCACGGTCGGCCTCGGCACGGACCCCTGTCCCACGAACCG 178
****** * ****** ********* *********************************
BB_CT-36 GACCTGTCAAGGCCCCAATGGCACCAAGTTTGCTGCATCGGTCAACAACGCCTCCTTTGT 236
chr9 AACCTGTCAAGGCCCCAATGGCACCAAGTTTGCTGCATCGGTCAACAACGCCTCCTTTGT 238
***********************************************************
BB_CT-36 GTTCCCCCCCACTGCTTTTCTCCAGGCTCACTACTACAATCAGTCGAACGGGGTCTACAC 296
chr9 GTTTCCCTCCACTGCTCTTCTCCAGGCTCACTACTACTCGCAGTCGAACGGGGTCTACAC 298
*** *** ******** ******************** ********************
BB_CT-36 CACAGACTTCCCGGACATCCCTCCGTTCCCCTTCAACTACACGGGGACTCCACCCAACAA 356
chr9 CACAGACTTCCCGGACATCCCTCCGTTCCCCTTCAACTACACGGGGACTCCACCCAACAA 358
************************************************************
BB_CT-36 CACGTTCGTGAGCAAGGGAACCAAGGTGGTGGTGCTTCCGTTCAACACCAGCGTGGAGTT 416
chr9 CACGTTCGTGAGCAAGGGAACCAAGGTGGTGGTGCTTCCGTTCAACACCAGCGTGGAGTT 418
************************************************************
BB_CT-36 GGTGCTGCAGGACACCAGCATTCAAGGAATCGAGAACCATCCGATGCACTTCCATGGCCA 476
chr9 GGTGCTGCAGGACACCAGCATTCAAGGAATCGAGAACCACCCGATGCACCTCCATGGCCA 478
*************************************** ********* **********
BB_CT-36 CAACTTCCACGTGGTGGGACAAGGCTTCGGAAACTATGACCCGGTAACCGAGCCTTGTAA 536
chr9 CAACTTCCACGTGGTGGGACAAGGCTTCGGAAACTATGACCCGGTGACCGATCCTTCTAA 538
********************************************* ***** **** ***
BB_CT-36 CTTCAATCTCGTCGACCCTGTGGAGAAGAACACCGCCGGCGTCCCGGTGGGCGGATGGCT 596
chr9 CTTCAATCTCGTCGACCCCGTGGAGAAGAACACCGCCGGCGTCCCGGTCGGCGGATGGCT 598
****************** ***************************** ***********
BB_CT-36 CGCCATTCGCTTCCGAGCTGACAACCCCGGTAAGTGACAGATACTACTATCTTCACTGGT 656
chr9 CGCCATTCGCTTCCGAGCTGACAACCCCGGTAAGTGA----TACTACTATCTTCAGTGGT 654
************************************* ************** ****
BB_CT-36 GGTTTCTTTAGTTGCTGGACGTTGACTTGGGTACGGAACAGGTGCATGGCTCATGCACTG 716
chr9 GGTTTCTTTAGTTGCTG-ACGTTGACTTGGGTACGTAACAGGTGCATGGCTCATGCACTG 713
***************** ***************** ************************
BB_CT-36 CCACATCGACTTGCATCTCAGCTGGGGGTTGAAGATGGTGTGGGTGGTCAACGACGGGCC 776
chr9 CCACATCGACTTGCATCTCAGCTGGGGATTGAAGATGGTGTGGGTGGTCAACGACGGGCC 773
*************************** ********************************
BB_CT-36 ACTCCCCAACCAGAAGCTCCCTCCTCCACCGTCCGATCTTCCAAGATGTTGATGCTGACC 836
chr9 ACTCCCCAACCAGAAGCTCCCTCCTCCGCCGTCCGATCTTCCAAGATGTTGATTCTGACC 833
*************************** ************************* ******
BB_CT-36 GCTGGATCGGTCGCGTGTGTTCTGGGGCTTGCTTGGTTGGAATTTTGTCGTGGTGTATCG 896
chr9 GCTGGATCGGTCGCCTGTGTCCTGGGTCTTGCT-GGTTGGTATTTTGTCGTGGTGTATCG 892
************** ***** ***** ****** ****** *******************
BB_CT-36 AAACTTACTACGTCTCTTCTCTCTCTCTCTCTCTCTCTCTCTCCCCGCCTACTGTTGCGG 956
chr9 AAACTTACTACGTATCTTCTCTCTCTCTCTCTCTCT----CTCCCCGCCTACTGTTGCGG 948
************* ********************** ********************
BB_CT-36 TCAAAATTGGGTTTCCTTATTTTGAACTGTAAATTCCCACATCACAAGCTCCCAGATTAG 1016
chr9 TCAAAATTGGGTTTCCTTATTTTGCACTGTAAATTTCCACATCACAAGCTCCCAAATTAG 1008
************************ ********** ****************** *****
BB_CT-36 ACTTTTTGATATTGTTCTTGCTGGTTTTCCAATTGAATAAAAATAAAATTGGTTCCTGCC 1076
chr9 ACTTTTTGATCTTGTTCTTGCTGGTTTTCCAATTGAA------TAAAATTGGTTCCTGCC 1062
********** ************************** *****************
Banana SSR markers – Primers Set 7
PET / BB_CT-6
F: GGCTTGGTCATCAGAGGAAG 5’to3’
R: TGAAGCCAAACCTTTATTGC 5’to3’
SSR-enriched library clone sequence
Accession KM190174
>BB_CT-6
TACCACATTGGTCCATGAAAAAATAGATTTCACTCTTCTTATCATGTAACTTCCAGTTACACTTACTTAGTATTCTCACCAACCCTTCTCTGTTTATTCTGCAGAGTATTTCACCACATTTATCCTTGGGCACTGAAATATTTTGGCTTGGTCATCAGAGGAAGTCTGGTATTGGTTTTGCTGCTCGGTACAATACTGATAATATGGTAAATCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCACAGACACACACACCCTCCCAAACGGTAAATAATTGCAATAAAGGTTTGGCTTCATTTTTG
Musa balbisiana ‘Pisung Klutuk Wulung’ sequence at Banana Genome Hub
>chr10:9116593..9116912
CAAAAATGAAGCCAAACCTTTATTGCAATTATTTACCGTTTGGGAGGGTGTGTGTGTCTGTGTCTGTGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGATTTACCATATTATCAGTATTGTACCGAGCAGCAAAACCAATACCAGACTTCCTCTGATGACCAAGCCAAAATATTTCAGTGCCCAAGGATAAATGTGGTGAAATACTCTGCAGAATAAACAGAGAAGGGTTGGTGAGAATACTAAGTAAGTGTAACTGGAAGTTACATGATAAGAAGAGTGAAATCTATTTTTTCATGGACCAATGTGGTA
CLUSTAL 2.1 multiple sequence alignment
Note: Sequence of BB_CT-6 clone is reverse complemented
BB_CT-6 CAAAAATGAAGCCAAACCTTTATTGCAATTATTTACCGTTTGGGAGGGTGTGTGTGTCTG 60
chr10 CAAAAATGAAGCCAAACCTTTATTGCAATTATTTACCGTTTGGGAGGGTGTGTGTGTCTG 60
************************************************************
BB_CT-6 TG------AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGATTTACCATATT 110
chr10 TGTCTGTGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGATTTACCATATT 120
** ************************************************
BB_CT-6 ATCAGTATTGTACCGAGCAGCAAAACCAATACCAGACTTCCTCTGATGACCAAGCCAAAA 170
chr10 ATCAGTATTGTACCGAGCAGCAAAACCAATACCAGACTTCCTCTGATGACCAAGCCAAAA 180
************************************************************
BB_CT-6 TATTTCAGTGCCCAAGGATAAATGTGGTGAAATACTCTGCAGAATAAACAGAGAAGGGTT 230
chr10 TATTTCAGTGCCCAAGGATAAATGTGGTGAAATACTCTGCAGAATAAACAGAGAAGGGTT 240
************************************************************
BB_CT-6 GGTGAGAATACTAAGTAAGTGTAACTGGAAGTTACATGATAAGAAGAGTGAAATCTATTT 290
chr10 GGTGAGAATACTAAGTAAGTGTAACTGGAAGTTACATGATAAGAAGAGTGAAATCTATTT 300
************************************************************
BB_CT-6 TTTCATGGACCAATGTGGTA 310
chr10 TTTCATGGACCAATGTGGTA 320
********************