This Antibody Is Intended for Use to Qualitatively Identify Δnp63(P40) Isoforms by Light

/
Rev: A
Release Date: 11/4/2016
IVD
Clone / ZR8
Source / Rabbit Monoclonal
Cat # / PR256-6ml RTU
PR256-3ml RTU
CR256-0.1ml Conc
CR256-0.5ml Conc
HAR256-6ml RTU
HAR256-3ml RTU
Regulatory Status / IVD

p40 (ΔNp63)

Intended Use:

This antibody is intended for use to qualitatively identify ΔNp63(p40) isoforms by light microscopy in formalin fixed, paraffin embedded tissue sections using immunohistochemical detection methodology. Interpretation of any positive or negative staining must be complemented with the evaluation of proper controls and must be made within the context of the patient’s clinical history and other diagnostic tests. A qualified pathologist must perform evaluation of the test.

Summary and Explanation:

p63 consists of two major isoforms-TAp63 and ΔNp63. These isoforms differ in the structure of the N terminal domains. The TAp63 isoform (identified by anti-p63 antibody) contains a transactivationcompetent ‘TA’ domain with homology to p53, which regulates the expression of the growth-inhibitory genes. In contrast, ΔNp63 isoform (identified by anti-p40 antibody) contains an alternative transcriptionally-inactive ‘ΔN’ domain, which antagonizes the activity of TAp63 and p53. The p40 (clone ZR8) antibody recognizes exclusively ΔNp63 but not TAp63. p40 is a squamous cell carcinoma ‘specific’ antibody. It reacts with the vast majority of cases of squamous cell carcinomas of various origins, but not with adenocarcinomas. It is particularly useful in differentiating lung squamous cell carcinoma from lung adenocarcinoma. p40 antibody can also be used as an alternative basal cell/myoepithelial cell marker, which has similar sensitivity and specificity as that of p63 antibody. Therefore, p40 antibody may also be used as an alternative immunohistochemical marker for determining prostate adenocarcinoma vs. benign prostate glands and for determining breast intraductal carcinoma vs. invasive breast ductal carcinoma.

Immunogen: Synthesized polypeptides from N-terminal domain of p63

Isotype: Rabbit IgG

Reagent Provided:
Concentrated format: Antibody to p40(ΔNp63) is diluted in antibody diluent, with 1% bovine serum
albumin (BSA) and 0.05% sodium azide (NaN3). Recommended dilutions: 1:50 –
1:100.The antibody dilution and protocol may vary depending on thespecimen
preparation and specific application. Optimal conditions should be
determined by individual laboratory.

Pre-diluted format: PathnSitu ready to use antibodies are pre tittered to optimal staining
conditions. Further dilution may loose the activity and may yield to sub
optimal staining.

Storage Recommendations: Store at 2°-8°C. Do not use after expiration date provided on the vial.

Staining Recommendations:

Antigen Retrieval Solution: Use Tris -EDTA Buffer(PathnSitu Cat # PS009) as antigen retrieval
solution Heat Retrieval Method: Retrieve sections under steam pressure
for 15 min using PathnSitu’s MERS (Multi Epitope Retrieval System) then
allow solution to cool for 10 minutes then transfer tissue sections/slides to
distilled water.

Primary Antibody: Cover the tissue sections with primary antibody and incubate for 30
min at room temperature when used PathnSitu PolyExcel Detection
System.

Detection System: Refer to PathnSitu PolyExcel detection system protocol or manufacturer’s

detection kit staining protocol when used other vendor detection system.

Cellular Localization: Nuclear

Positive Control: Lung Squamous cell carcimona

Troubleshooting: Follow the antibody specific protocol recommendations according to data sheet provided. If unusual results occur, contact PathnSitu Technical Support at 040-2701 5544 or

Limitations and Warranty: There are no warranties, expressed or implied, which extend beyond this
description. PathnSitu is not liable for property damage, personal injury, or
economic loss caused by this product.

Bibliography: 1. Guojun Wu et al. (2003) ΔNp63α and TAp63α Regulate Transcription of Genes with Distinct Biological Functions in Cancer and Development Cancer Res 2003 63; 2351.
2. Justin ABishop et al. (2011) p40 (ΔNp63) is superior to p63 for the diagnosis of pulmonary squamous cell carcinoma. Modern Pathology 2012, 405–415
3. Verena Sailer et al.Comparison of p40 (ΔNp63) and p63 expression in prostate tissues – which one is the superior diagnostic marker for basal cells? Histopathology 2013, 50–56

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