Appendix
Appendix1 Information about the 26 tributaries in TGRC
River name / Abbreviation / District/county / Location into the Yangtze River / Watershed area（km2） / Length within the reservoir area（km） / Annual flow（m3/s） / Distance from the Three Gorges Dam(km)Qijiang River / QJ / Jiangjin / Shunjiang / 4394 / 153 / 122 / 654
Jialin River / JL / Yuzhong / Chaotianmen / 157900 / 153.8 / 2120 / 604
Wubu river / WB / Banan / Mudong / 858.2 / 80.8 / 12.4 / 573.5
Yulin River / YL / Yubei / Luozexinhua / 908 / 58.4 / 50.7 / 556.5
Longxi River / LX / Changshou / Yangjiaobao / 3248 / 218 / 54 / 526.2
Lixiangxi river / LXX / Fulin / Ningshi / 850.6 / 13.6 / 13.6 / 506.2
Wujiang River / WJ / Fulin / Maliuzui / 87920 / 65 / 1650 / 484
Quxi River / QX / Fengdu / Quxi / 923.4 / 93 / 14.8 / 459
Long River / LH / Fengdu / Wuyang / 2810 / 114 / 58 / 429
Huangjin River / HJ / Zhongxian / Hongxin / 958 / 71.2 / 14.3 / 361
Ruxi River / RX / Zhongxian / Shibao county / 720 / 11.9 / 11.9 / 337.5
Xiaojiang River / XJ / Yuyang / Shuangjiang / 5172.5 / 117.5 / 116 / 247
Tangxi River / TX / Yuyang / Yunyang / 1810 / 108 / 56.2 / 222
Modaoxi River / MD / Yuyang / Xinghe / 3197 / 170 / 60.3 / 218.8
Changtan River / CT / Yuyang / Guling / 1767 / 93.6 / 27.6 / 206.8
Meixi River / MX / Fengjie / Fengjie / 1972 / 112.8 / 32.4 / 158
Caotangxi River / CTX / Fengjie / Baidicheng / 394.8 / 31.2 / 8 / 153.5
Daxi River / DXH / Wushan / Daxi / 158.9 / 85.7 / 30.2 / 146
Daning River / DL / Wushan / Wushan / 4200 / 142.7 / 98 / 123
Guandu River / GD / Wushan / Qingshi / 315 / 31.9 / 6.2 / 110
Baolong River / BL / Wushan / Butou / 325 / 22.3 / 6.6 / 106.5
Shenglongxi River / SL / Badong / Guandukou / 350 / 60 / 20 / 74
Tongzhuang River / TZ / Zigui / Dengjiaba / 248 / 36.6 / 6.4 / 42
Zhaxi River / ZX / Zigui / Guizhou / 193.7 / 52.4 / 8.3 / 34
Xiangxi River / XX / Zigui / Xiangxi / 3095 / 110.1 / 47.4 / 32
Jiuwanxi River / JW / Zigui / Jiuwanxi / 514 / 42.1 / 17.5 / 20
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Appendix 2 Information of the 57 sampling sites in 26 tributaries in TGRC
Code / Group / Within the river / Location / Latitude/Longitude / Dams present or absent / Elevation / Distance from the river mouth / Temperature / DOmg/L / pHQX1 / Reference / Quxi river / Upstream / 30.241222°/107.798327° / NO / 249 / 81 / 20.39 / 6.85 / 7.95
GD2 / Reference / Guandu River / Middlestream / 30.950447°/109.842374° / NO / 307 / 24.4 / 18.3 / 9.17 / 8.53
BL1 / Reference / Baolong River / Upstream / 30.898209°/109.970935° / No / 282 / 17.6 / 16.1 / 9.13 / 8.58
BL2 / Reference / Baolong River / Downstream / 30.949302°/110.012556° / No / 203 / 8.1 / 16.2 / 9.83 / 8.63
TZ1 / Reference / Tongzhuang Rver / Upstream / 30.809472°/110.645224° / No / 448 / 22.8 / 11.6 / 9.83 / 8.4
TZ2 / Reference / Tongzhuang Rver / Downstream / 30.909242°/110.666166° / No / 193 / 9.8 / 12.6 / 12.68 / 8.17
DXH1 / Reference / Daxi River / Upstream / 30.857528°/109.383287° / No / 346 / 33.5 / 17.52 / 7.93 / 8.26
MX1 / Reference / Meixi River / Upstream / 31.202349°/109.136633° / No / 410 / 57.7 / 14.55 / 7.64 / 8.17
MX2 / Reference / Meixi River / Middlestream / 31.130045°/109.201935° / No / 252 / 42.2 / 16.24 / 7.77 / 8.31
MX3 / Reference / Meixi River / Downstream / 31.120977°/109.338595° / No / 178 / 27 / 16.43 / 7.84 / 8.25
ZX1 / Reference / Zhaxi River / Upstream / 31.224515°/110.547892° / No / 783 / 39.4 / 17 / 9.72 / 8.53
DL1 / Reference / Daning River / Upstream / 31.606006°/109.167963° / Above Zhongliang dam 19.5km / 742 / 129.7 / 17.1 / 9.28 / 8.67
DL2 / Reference / Daning River / Middlestream / 31.532542°/109.623862° / Below Zhongliang dam 30.9km / 282 / 79.7 / 16.7 / 10.04 / 8.75
DL3 / Reference / Daning River / Downstream / 31.323588°/109.625914° / No / 202 / 53.7 / 17.3 / 9.64 / 9.31
MD3 / Reference / Modaoxi River / Upstream / 30.613245°/108.642458° / Above Shuanghe dam 10km / 378 / 74.4 / 16.06 / 8.43 / 8.91
HJ1 / Reference / Huangjin River / Upstream / 30.468170°/107.959558° / No / 275 / 12.3 / 22.92 / 9.69 / 8.5
HJ2 / Reference / Huangjin River / Middlestream / 30.443486°/107.988543° / No / 262 / 18.5 / 22.79 / 8.93 / 8.21
HJ3 / Reference / Huangjin River / Downstream / 30.394663°/107.994232° / No / 213 / 23.6 / 23.06 / 9.11 / 8.55
RX1 / Reference / Ruxi River / Upstream / 30.594594°/107.971826° / No / 245 / 33.3 / 22.74 / 7.64 / 8.25
RX2 / Reference / Ruxi River / Middlestream / 30.508500°/108.044256° / No / 237 / 17.3 / 24.9 / 11.1 / 8.51
CT1 / Reference / Changtan River / Upstream / 30.721549°/109.015401° / No / 290 / 35.5 / 14.99 / 8.16 / 8.05
CTX1 / Reference / Caotabgxi River / Upstream / 31.131582°/109.516364° / No / 326 / 7.4 / 15.5 / 7.32 / 8.18
JW1 / Reference / Jiuwanxi River / Upstream / 30.739311°/110.712840° / No / 488 / 27.2 / 14.4 / 10.15 / 8.71
JW2 / Reference / Jiuwanxi River / Middlestream / 30.762002°/110.773244° / No / 343 / 20 / 15.3 / 10.32 / 8.65
SL2 / Reference / Shenglongxi River / Middlestream / 31.300297°/110.300154° / No / 288 / 33.6 / 13.9 / 9.75 / 8.59
SL3 / Reference / Shenglongxi River / Downstream / 31.238156°/110.301594° / No / 189 / 24.7 / 14.5 / 9.86 / 8.49
XJ1 / Reference / Xiaojiang-Dongjiang River / Upstream / 31.551137°/108.671767° / No / 650 / 145.5 / 13.05 / 7.5 / 7.9
TX1 / Reference / Tangxi River / Upstream / 31.402661°/108.863902° / No / 319 / 53.5 / 15.05 / 7.35 / 8.2
TX2 / Reference / Tangxi River / Middlestream / 31.312191°/108.859545° / No / 238 / 44.7 / 14.52 / 8.1 / 8.17
SL1 / Impaired / Shenglongxi River / Upstream / 31.345827°/110.258725° / No / 758 / 43.6 / 14.3 / 9.4 / 8.45
CTX2 / Impaired / Caotabgxi River / Downstream / 31.095405°/109.559437° / No / 209 / 14.1 / 16.78 / 7.76 / 8.46
CT2 / Impaired / Changtan River / Middlestream / 30.826460°/109.048602° / Below Yanpin dam 0.9km / 269 / 19.5 / 16.72 / 7.86 / 8.14
CT3 / Impaired / Changtan River / Downstream / 30.934493°/109.085240° / No / 110 / 3.5 / 16.82 / 8.12 / 8.14
RX3 / Impaired / Ruxi River / Downstream / 30.478793°/108.090331° / No / 227 / 11 / 27.53 / 14.47 / 9.01
MD1 / Impaired / Modaoxi River / Downstream / 30.777158°/108.783111° / Below Menkantan dam 1km / 197 / 33.4 / 14.84 / 7.61 / 8.17
MD2 / Impaired / Modaoxi River / Middlestream / 30.742017°/108.613549° / Below Changtan dam 1.2km / 261 / 52.4 / 15.14 / 10.06 / 8.61
ZX2 / Impaired / Zhaxi River / Downstream / 31.092350°/110.655862° / No / 266 / 12.6 / 12.6 / 11.88 / 8.62
DXH2 / Impaired / Daxi River / Middlestream / 30.772524°/109.480017° / No / 310 / 36 / 13.4 / 9.22 / 8.82
DXH3 / Impaired / Daxi River / Downstream / 30.891244°/109.560980° / No / 230 / 18.4 / 15.1 / 9.02 / 8.47
GD3 / Impaired / Guandu River / Downstream / 31.001952°/109.934149° / NO / 268 / 8.1 / 16.9 / 9.28 / 8.65
QX2 / Impaired / Quxi river / Middlestream / 30.130777°/107.684835° / NO / 221 / 57 / 14.01 / 10.67 / 8.3
GD1 / Impaired / Guandu River / Upstream / 30.903448°/109.717668° / Above a small dam 1km / 803 / 38.8 / 19.4 / 8.52 / 8.58
TX3 / Impaired / Tangxi River / Downstream / 31.211563°/108.810671° / No / 175 / 35.7 / 15.61 / 7.13 / 8.08
WB2 / Impaired / Wubu River / Middlestream / 29.421093°/106.876001° / Above Yutan dam 10km / 249 / 16.5 / 17.78 / 9.8 / 12
QJ1 / Impaired / Qijiang River / Upstream / 29.094964°/106.407287° / Below Wufu dam 9.2km / 196 / 33.4 / 16.3 / 12.01 / 6.95
QJ2 / Impaired / Qijiang River / Downstream / 29.172138°/106.379727° / NO / 191 / 19.4 / 16.71 / 12.37 / 8.14
YL1 / Impaired / Yulin River / Upstream / 29.858371°/106.845263° / NO / 186 / 52 / 17.4 / 11.3 / 10.06
YL2 / Impaired / Yulin River / Middlestream / 29.807188°/106.879952° / Below Shuangtanzi dam 33.5km / 183 / 40.8 / 18.49 / 11.47 / 10.39
YL3 / Impaired / Yulin River / Downstream / 29.770048°/106.863182° / NO / 175 / 32 / 17.34 / 10.92 / 8.58
LX1 / Impaired / Longxi River / Upstream / 30.061164°/107.207020° / Above Shizitan dam 27.5km / 338 / 55.8 / 17.54 / 11.96 / 7.29
XX1 / Impaired / Xiangxi River / Upstream / 31.444446°/110.820265° / Above Gudongkouyiji dam 13km / 336 / 40.8 / 17.4 / 11.52 / 8.66
XX2 / Impaired / Xiangxi River / Downstream / 31.278059°/110.744550° / Below Gudongkouerji dam 1.3km / 172 / 25.5 / 17.4 / 17.51 / 9.27
LXX1 / Impaired / Lixiangxi River / Upstream / 29.627454°/107.071260° / Below Geyangqiao dam 6.3km / 178 / 10 / 15.57 / 12.38 / 6.34
LXX2 / Impaired / Lixiangxi River / Downstream / 29.630758°/107.096780° / Below Geyangqiao dam 2.8km / 172 / 7.7 / 14.94 / 12.7 / 6.2
LH1 / Impaired / Long River / Upstream / 29.955414°/108.085018° / Above Shiban dam 26 km and below Tongzigou dam 31.5km / 528 / 43 / 11.64 / 11.23 / 8.03
WJ1 / Impaired / Wujiang River / Downstream / 29.259033°/107.883563° / Below Yinpan dam 1km / 196 / 54.6 / 13.09 / 14.34 / 8.05
JL1 / Impaired / Jialing River / Upstream / 30.177425°/106.233003° / Downstream 81km Qinju dam and upstream 44km Caojie dam / 200 / 87.8 / 15.9 / 11.14 / 8.06
Appendix 3 The definitions of candidate metrics and their expected response to perturbation
No. / Metrics code / Definition. / Expected response to increasing perturbationComposition/abundance measures
1 / PMA* / Percent model affinity / Decrease
2 / BivalPct / Percent of individuals of bivalvia / Decrease
3 / ChiroPct / Percent of individuals of midge larvae / Decrease
4 / ColeoPct / Relative abundance of beetle larvae and aquatic adults / Decrease
5 / CrMolPct / Relative abundance of individuals classed as crustaceans or molluscs. / Decrease
6 / DipPct / Percent of all “ture” fly larvae / decrease
7 / GastrPct / Relative abundance of snails / Increase
8 / OdonPct / Percent of individuals of odonata / Increase
9 / OligoPct / Percent of individuals of oligochaeta / Increase
10 / IsoPct / Relative abundance of crustaceans in the order Isopoda / Increase
11 / EphemPct / Percentage of individuals of ephemeroptera / Decrease
12 / EPTPct / Percent composition of mayfly, stonefly, and caddisfly larvae / Decrease
13 / TrichPct / Percentage of individuals in caddisflies / Decrease
14 / PlecoPct / Percent of individuals of stonefly nymphs / Decrease
15 / TanytPct / Percent of individuals of tanypodinae / Decrease
Sensitivity/tolerance measures
1 / HBI* / Hilsenhoff biotic index / Increase
2 / Dom01Pct* / Percentage of individuals of 1st dominant taxa / Increase
3 / NonInPct / Percent of individuals in non-insects / Increase
4 / IntolPct / Percent of individuals in taxa with PTV(pollution tolerant value)<3 / Decrease
5 / IntolTax* / Number of taxa with PTV<3 / Decrease
6 / TolerPct / Percent of individuals in taxa with PTV>7 / Increase
7 / TolerTax / Number of taxa with PTV>7 / Increase
8 / Tnyt/ChiPct / Percent composition within the midge family that are tanypodinae / Vary
9 / CrCh/ChiPct / Percent composition within the midge family that are cricotopus and chironomus / Increase
10 / Orth/ChiPct / Percent composition within the midge family that are orthocladiinae / Increase
11 / Baet/EphPct / Percent pollution tolerant mayflies (baetidae) of all mayflies. / Increase
12 / Hyd/EPTPct / Percent of pollution tolerant caddisflies (hydropsychidae) to all mayflies, stoneflies and caddisflies. / Increase
13 / Hyd/TriPct / Percent of hydropsychidae to trichoptera / Increase
Functional measures
1 / CllctPct / Percentage of the collector functional feeding group / Vary
2 / CllctTax* / Number of taxa of the collector functional feeding group / Vary
3 / FiltrPct / Percent composition of the filterer functional feeding group / Decrease
4 / FiltrTax / Number of taxa in the filterer functional feeding group / Decrease
5 / PredPct / Percent composition of the predator functional feeding group / Decrease
6 / PredTax / Number of taxa of the predator functional feeding group / Vary
7 / ScrapPct / Percent of individuals in the scraper functional feeding group / Decrease
8 / ScrapTax / Number of taxa of the scraper functional feeding group / Decrease
9 / ShredPct / Percent of individuals in the shredder functional feeding group / Decrease
10 / ShredTax / Number of taxa of the shredder functional feeding group / Decrease
Richness/diversity measures
1 / TotalTax* / Total number of taxa in the sample / Increase
2 / ChiroTax / Number of taxa of chironomid(midge) larvae / Decrease
3 / ColeoTax / Number of taxa of beetle larvae and aquatic adults / Decrease
4 / CrMolTax / Number of taxa of crustaceans or molluscs / Decrease
5 / DipTax / Number of “ture” fly taxa, which includes midges / Decrease
6 / OligoTax / Number of taxa in the order Oligochaeta / Increase
7 / OrthoTax / Species number of orthocladiinae / Decrease
8 / TanytTax / Species number of tanypodinae / Decrease
9 / EphemTax* / Number of taxa in the insect order Ephemeroptera / Decrease
10 / EPTTax* / Number of taxa in the insect orders Ephemeroptera, Plecoptera, and Trichoptera. / Decrease
11 / TrichTax / Number of taxa in caddisflies. / Decrease
12 / Shan_2* / Shannon-Wiener diversity index / Decrease
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