The University of Texas Medical Branch Clinical Chemistry Objectives

The University of Texas Medical Branch Clinical Chemistry Objectives

School of Allied Health Sciences Page 1 of 4

Department of Clinical Laboratory Sciences Revised 10/3/18

Clinical ChemistryPRECEPTORSHIP

OBJECTIVES

Purpose

The UTMB CLS student is required to meet cognitive (knowledge), psychomotor (skill) and affective (attitude) objectives of each preceptorship. This document outlines the cognitive and psychomotor objectives of the program. Cognitive goals are evaluated through the use of a quiz based on the study guide questions, which are based on these preceptorship objectives.

Preceptorship Objectives

Upon successful completion of this course, at a level of 70% the student will be able to:

GENERAL PROCEDURES / Objective met
Safety

1. Use appropriate personal protective equipment at all times when working with patient samples.

1. Locate all fire extinguishers, eye wash stations and safety showers.

1. Locate Materials Safety Data Sheets, chemicals carcinogens list, and hazardous chemicals list.

Specimen Preparation

1. Recognize unacceptable specimens and follow procedures required when such specimens are received.

1. Collect or recognize and process without assistance appropriate specimens for testing.

1. Separate, aliquot, label and distribute specimen according to laboratory standard operating procedure (SOP).

1. Organize patient workload and manage time to complete task assignments in specific time allowed.

1. Apply requirements for proper urine collection and preservation to sample processing.

1. Dilute specimens, repeat analysis and calculate true result for all procedures performed as required.

Reference Laboratory Send-Out

1. Using reference laboratory procedure manuals, process specimens for transport.

Laboratory Information System (LIS)

1. Use or observe LIS to enter patient demographic and laboratory results.

Quality Assurance

1. Accurately and legible log in and maintain required records at all times.

1. Pipette accurately at all times.

1. Reconstitute control sera and standards with accuracy and reliability without direct supervision.

1. Perform quality assurance procedures for each test analysis and consistently maintain required documentation.

1. Evaluate quality control data, recognize out-of-control data and perform troubleshooting measures according to laboratory policies for all laboratory procedures.

1. Apply Westgard rules to quality control decisions, recognize out-of-control situations, and perform actions outlined in the SOP when these situations occur.

1. Determine and implement the course of action to be taken when delta checks are not correlated.

1. Recognize test results that need to be checked by repeat testing and those that are beyond the limits of linearity and perform procedures as defined by SOP when these occur.

ReferenceRanges

1.Recognize reference ranges, therapeutic ranges, and critical values and perform procedures at all times that are required by the SOP when these occur.

AUTOMATION

End-Point and Kinetic Spectrophotometric Analysis

1.Perform end-point chemistry analysis accurately on patient specimens using the automated chemistry analyzer.

1. Perform kinetic analysis of body fluids, such as blood, pericardial or pleural

fluid.

3.Perform instrument functional check procedures as required for each instrument, maintaining required documentation at all times.

4.Evaluate test results for their validity.

5.Perform daily and weekly maintenance and troubleshooting on automated chemistry instruments.

6.Maintain instrument logs as required on automated instruments.

1. Discuss special sample preparation and handling for tests such as ammonia and lactate.

Electrochemistry

1. Perform analysis of patient specimens by the ion-selective electrode analyzer.

1. Perform or verify calibration or standardization of electrochemical method.

1. Recognize electrochemistry instrument problems and warnings and perform procedures to correct these problems.

1. Following instructor demonstration and using the electrochemistry instrument manual and maintenance manual, perform, with minimal supervision, maintenance and troubleshooting procedures assigned by the instructor.

Indicator-labelled Immunoassay

1. Perform analysis of patient specimens by automated immunoassay analyzer.

1. Perform or verify calibration or standardization of immunoassay method.

1. Recognize instrument problems and warnings and perform procedures to correct these problems.

1. Following instructor demonstration and using the instrument manual and maintenance manual, perform, with minimal supervision, maintenance and troubleshooting procedures assigned by the instructor.

SPECIAL CHEMISTRY

Immunochemical Assay (Immunodiffusion, Nephelometry/turbidimetry)

1. Discuss/observe immunofixation procedures.
2. Discuss/observe immunodiffusion patterns, with interpretation.
3. Discuss/observe nephelometry and turbidometric procedures.

Electrophoresis

1.Discuss/observe serum protein electrophoresis (SPE), the measurement of separated fractions, and procedures for identifying the five major protein fractions in the order in which they migrate on the electrophoresed agarose gel.

2.Discuss/observe densitometric quantitation of separated electrophoresis fractions.

3.Discuss/observe steps needed to prepare CSF and urine samples as well as other adaptations needed for protein electrophoresis of body fluids.

Chromatography
1. discuss maintenance procedures for analyzing body fluids by chromatography.
2. discuss chromatography procedures, including reagent and sample preparation.

Osmometry

1.Discuss/observe the analysis of patient urine and serum specimens by osmometry.

2.Discuss/observe calibration or standardization of the osmometry instrument.

3.Recognize instrument problems and warnings and discuss procedures to correct these problems.

ACID BASE/ABG’s

1.Discuss/observe the analysis of patient specimens by the analyzer.

2.Discuss/observe the process of calibration or standardization of electrochemical methods.

3.Discuss/observe instrument problems and warnings and procedures to correct these problems.

4.Discuss/observe maintenance and troubleshooting procedures assigned by the instructor.

POINT OF CARE TESTING (POCT)

1.Discuss/observe the analysis of body fluids at the bedside.

2.Discuss/observe calibration or standardization of the POCT instrument.

3.Recognize instrument problems and warnings and discuss procedures to correct these problems.

4.Following instructor demonstration and using the instrument manual and maintenance manual, perform, with minimal supervision, maintenance and troubleshooting procedures assigned by the instructor.

Urinalysis PRECEPTORSHIP
OBJECTIVES

Purpose

The UTMB CLS student is required to meet cognitive (knowledge), psychomotor (skill) and affective (attitude) objectives of each preceptorship. This document outlines the cognitive and psychomotor objectives of the program. A checklist for evaluation of the psychomotor objectives is included in this document. Cognitive goals are evaluated through the use of study guide questions, which are based on the preceptorship objectives.

Urinalysis Preceptorship Objectives

Upon successful completion of this course, at a level of 70% the student will be able to:

I. Automated Urinalysis

1. perform the analysis of patient urine specimens using the automated system.

2.perform or verify calibration or standardization of the instrument.

3.recognize instrument problems and warnings and perform procedures to correct these problems.

4.following instructor demonstration and using the instrument manual and maintenance manual, perform, with minimal supervision, maintenance and troubleshooting procedures assigned by the instructor.

1. following instructor demonstration and using the procedure manual, perform without direct assistance, urine sample preparation prior to testing.

II. Physical and Chemical Urine Testing

1. following instructor demonstration and using the procedure manual, perform without direct assistance, urine sample preparation prior to testing.

1. using the procedure manual, perform without direct assistance assessment of physicalcharacteristics of urine.

1. using the procedure manual, perform without direct assistance, manual chemical testing of urine

1. using the procedure manual, perform without direct assistance, reducing substance

III. Microscopic Urine Exam

1. using the procedure manual, perform without direct assistance, urine samplepreparation prior to microscopic examination

1. using the procedure manual, perform with minimal supervision, proper adjustment ofthe microscope to be used for microscopic exam.

1. using the procedure manual, perform with minimal supervision, routine microscopic urine exam.

IV. Urine Pregnancy Test

1. using the procedure manual, perform without direct assistance, urine sample preparation prior to pregnancy testing

1. using the procedure manual, and following instructor demonstration, perform with minimal supervision, pregnancy testing of urine

.