The colonization history of the Mediterranean dwarf palm (Chamaerops humilis L., Palmae)

Beatriz Guzmán, José M. Fedriani, Miguel Delibes, Pablo Vargas

Appendix S1: Supplementary Tables S1-S3

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Table S1. Summary of the results from a pilot study (n = 5) performed to select a plastid region with the highest variation at the species level.

Sequence length / No. variable positions
rpL32
3'trnV-ndhC / 888
212 / 1
0
trnQ-50rps16 / 114 / 1
rpS16-trnK / 720 / 0
ndhF-rpL32
psbD-trnT
petA-psbJ
petL-psbE
atpI-atpH / 379
1071
1008
920
824 / 0
0
27
1
0

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Table S2. GenBank accession number of 218 individuals (29 localities) of Chamaerops humilis and Trachycarpus fortunei, as outgroup.

Population code / Accesion no.
Chamaerops humilis
Andratx / KX277228-KX277224
AR / KX277223-KX277221
Artá / KX277220-KX277211
AT / KX277210-KX277201
BE / KX277200-KX277195
BF / KX277194-KX277185
BI / KX277184-KX277177
BM / KX277176-KX277167
BP / KX277166-KX277164
BR / KX277163-KX277156
CC / KX277155-KX277146
CG / KX277145-KX277136
FE / KX277135-KX277126
GR / KX277125-KX277116
JS / KX277115-KX277106
MA / KX277105-KX277097
PI / KX277096-KX277090
PND / KX277089-KX277080
SA / KX277079-KX277070
Saler / KX277069-KX277060
SC / KX277059-KX277057
SI / KX277056, KX277055
SM / KX277054-KX277051
SN / KX277050-KX277046
Stanger / KX277045-KX277036
TKA / KX277035-KX277026
TTE1 / KX277025-KX277016
TTE2 / KX277015-KX277006
ZI
OUTGROUP
Trachycarpus fortunei / KX277005-KX277001
KX277229

Table S3. Analysis of molecular variance (AMOVA) based upon the ptDNA marker petA-psbJ variation surveyed in a total of 14 populations (140 individuals) of Chamaerops humilis. df = degrees of freedom, and SS = sum of squared deviations.

Source of variation / df / SS / Variance components / Total variance (%) / Fixation indices
Among regions
Among populations within regions
Within populations / 1
12
126 / 10.293
508.714
279.300 / - 0.4586
4.0176
2.2167 / - 7.94
69.56
38.38 / Fct = - 0.079
Fst = 0.616*
Fsc = 0.644*
Total / 139 / 798.307 / 5.65710

* P 0.001, 10,000 permutations.

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