TAK1 inhibitor5Z-7-oxozeaenol sensitizes neuroblastoma to chemotherapy
Yihui Fan1, †, Jin Cheng1, 2, †, Sanjeev A. Vasudevan3, †, Roma H. Patel3, Li Liang4, Xin Xu 1, Yanling Zhao1, Wei Jia4, Fengmin Lu2, Hong Zhang4, Jed G. Nuchtern 3, Eugene S. Kim3, Jianhua Yang1, *
1Texas Children's Cancer Center, Department of Pediatrics, Dan L. Duncan Cancer Center, Baylor College of Medicine,Houston, Texas 77030, USA
2Department of Microbiology, Peking University Health Science Center, Beijing 100191, China
3Michael E. DeBakey Department of Surgery, Division of Pediatric Surgery, Dan L. Duncan Cancer Center, Baylor College of Medicine,Houston, Texas 77030, USA
4Department of Pathology, University of Texas MD Anderson Cancer Center,Houston, Texas 77030, USA
Running head: TAK1 inhibition in neuroblastoma
† Yihui Fan, Jin Cheng and Sanjeev A. Vasudevancontributedequally to this work.
*Correspondence:
Conflict of Interest
The authors declare no conflict of interest.
Legend for Supplementary Figures
Figure S15Z-7-oxozeaenolenhances the cytotoxic effectof Dox and VP-16 on neuroblastoma cells. (A, B) NGP, (C, D) NB-19, (E, F)CHLA-255, (G, H)SK-N-AS cells were seeded into 96-well plates at a concentration of 1x104/well. After 24 hrs, cells were incubated with drugs for 24 hrs at indicated concentrations and cell viability was assessed by CCK-8 assay.Results presented as % vehicle± SD (n=6). * P<0.05, **P<0.01, ***P<0.001 (Student’s t test, two-tailed).
Figure S2Relative low doses of 5Z-7-oxozeaenoldo nothave obvious cytotoxic effect on neuroblastoma cells. IMR32, NGP, NB-19, CHLA-255, SK-N-AS and SH-SY5Y neuroblastoma cells were seeded in 96-well plates at a concentration of 1x104/well. After 24 hrs, cells were incubated with 5Z-7-oxozeaenol for 24 hrs at indicated concentrations and cell viability was assessed by CCK-8 assay.
Figure S35Z-7-oxozeaenolinhibitscolony formation of neuroblastoma cell. (A) IMR32 cells wereplated in 0.3% agarose/RPMI-1640 media containing 5Z-7-oxozeaenol with the indicated concentration on top of a 0.5% agarose/RPMI-1640 layer. After 14 days of growth, colonies were stained with MTTand counted. (B) Quantification of the results from A. These experiments were performed in sextuplicate and reported as the mean with SDs. ***P<0.001 (Student’s t test, two-tailed). (C) SH-SY5Y cells wereplated in 0.3% agarose/RPMI-1640 media containing 5Z-7-oxozeaenol with indicated concentration on top of a 0.5% agarose/RPMI-1640 layer. After 14 days of growth, colonies were stained with MTT andcounted. (D) Quantification of the results from C.
Figure S4Cytotoxic effect of DoxonLA-N-6 cells. LA-N-6 cells were seeded in 96-well plates at a concentration of 1x104/well. After 24 hrs, cells were incubated with drugs for 24 hrs at indicated concentrations and growth inhibition was assessed by CCK-8 assay.