Tables1. Putative Orfs on Cosmid Clone 9E7and Their Information (E Value0.01)

TableS1. Putative ORFs on cosmid clone 9E7and their information (E value0.01)

ORF / Homologous protein blast search information
Name / Length
(aa) / Homologous Protein / length (aa) / accession No. / Source / E-value / Identity(aa%) /
positive (aa%)
9E7-1 / >588 / RelA/SpoT protein (GTP diphosphokinase) / 735 / ZP_00866464 / Alkalilimnicola ehrlichei / 3e-165 / 299/592 (50%) / 425/592 (71%)
9E7-2 / 476 / Dihydrolipoamide dehydrogenase / 478 / YP_235092 / Pseudomonas syringae / 1e-156 / 275/475 (57%) / 357/475 (75%)
9E7-3 / 412 / SucB, dihydrolipoamide succinyltransferase, E2 subunit / 409 / CAH14810 / Legionella pneumophila str. Lens / 6e-108 / 227/414 (54%) / 311/414 (75%)
9E7-4 / 954 / Oxoglutarate dehydrogenase / 990 / YP_200683 / Xanthomonas oryzae pv. oryzae KACC10331 / 0.0 / 534/926 (57%) / 658/926 (71%)
9E7-5 / 88 / Unknown
9E7-6 / 45 / Unknown
9E7-7 / 168 / Cytochrome c family protein / 155 / NP_420024 / Caulobacter crescentus CB15 / 9e-21 / 45/100 (45%) / 63/100 (63%
9E7-8 / 424 / Alkane 1-monooxygenase / 404 / CAC38027 / Alcanivorax borkumensis / 8e-113 / 208/375 (55%) / 276/375 (73%)
9E7-9 / 54 / Rubredoxin 2 / 175 / CAB51049 / Pseudomonas putida / 4e-10 / 25/50 (50%) / 39/50 (78%)
9E7-10 / 272 / CAAX amino terminal protease family / 337 / ZP_00237276 / Bacillus cereus G9241 / 9e-05 / 51/184 (27%) / 87/184 (47%)
9E7-11 / 65 / Unknown
9E7-12 / 226 / Conserved hypothetical protein BF3725 / 249 / YP_101002 / Bacteroides fragilis YCH46 / 4e-09 / 58/225 (25%) / 99/225 (44%)
9E7-13 / 456 / Putative oxidoreductase / 452 / CAE31970 / Bordetella bronchiseptica RB50 / 1e-68 / 165/443 (37%) / 230/443 (51%)
9E7-14 / 165 / Unknown
9E7-15 / 392 / Hypothetical drug transport transmembrane protein / 400 / YP_130092 / Photobacterium profundum SS9 / 6e-52 / 135/344 (39%) / 202/344 (58%)
9E7-16 / 139 / Protein chain release factor B / 137 / ZP_00317715 / Microbulbifer degradans 2-40 / 3e-25 / 80/136 (58%) / 112/136 (82%)
9E7-17 / 444 / Probable transcriptional regulator / 667 / NP_773183 / Bradyrhizobium japonicum USDA 110 / 3e-14 / 57/181 (31%) / 96/181 (53%)
9E7-18 / 192 / Transcriptional regulator / 1113 / AAY47209 / Xanthomonas campestris pv. / 8e-17 / 46/102 (45%) / 62/102 (60%)
9E7-19 / 628 / TPR repeat / 560 / ZP_00262997 / Pseudomonas fluorescens PfO-1 / 5e-21 / 87/312 (27%) / 147/312(47%)
9E7-20 / 84 / Unknown
9E7-21 / 140 / Hypothetical protein CV1954 / 147 / AAQ59626 / Chromobacterium violaceum / 1e-14 / 42/129 (32%) / 64/129 (49%)
9E7-22 / 559 / Probable formate-tetrahydrofolate ligase protein / 568 / CAC46969 / Sinorhizobium meliloti / 0.0 / 365/555 (65%) / 451/555 (81%)
9E7-23 / 374 / Formaldehyde dehydrogenase / 473 / CAC85637 / 2e-31 / 114/351 (32%) / 169/351 (48%)
9E7-24 / 44 / Unknown
9E7-25 / 78 / Unknown
9E7-26 / 434 / Metal-dependent amidase / aminoacylase /carboxypeptidase / 445 / ZP_00170653 / Ralstonia eutropha / 5e-106 / 216/408 (52%) / 276/408 (67%)
9E7-27 / 385 / Conserved hypothetical protein / 372 / AAN57237 / Shewanella oneidensis MR-1 / 7e-116 / 214/350 (61%) / 274/350 (78%)
9E7-28 / 251 / Unknown
9E7-29 / 403 / Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit / 413 / ZP_00223612 / Burkholderia cepacia R1808 / 2e-61 / 143/414 (34%) / 205/414 (49%)
9E7-30 / 551 / Acyl-CoA synthetases (AMP-forming) / AMP-acid ligases II / 564 / ZP_00356116 / Chloroflexus aurantiacus / 1e-52 / 151/470 (32%) / 226/470 (48%)
9E7-31 / 334 / Hypothetical protein plu4509 / 351 / NP_931675 / Photorhabdus luminescens subsp. / 4e-04 / 60/242 (24%) / 99/242 (40%)
9E7-32 / 368 / Hypothetical protein (BtaA, S-adenosylmethionine:diacylglycerol 3-amino-3-carboxypropyl transferase) / 377 / BAD56289 / Nocardia farcinica IFM 10152 / 2e-25 / 89/347 (25%) / 161/347 (46%)
9E7-33 / 710 / Predicted exporters of the RND superfamily / 763 / ZP_00333969 / Thiobacillus denitrificans / 1e-15 / 154/578 (26%) / 253/578 (43%)
9E7-34 / 655 / Phosphoenolpyruvate synthase / pyruvate phosphate dikinase / 894 / CAE80911 / Bdellovibrio bacteriovorus HD100 / 2e-101 / 221/640 (34%) / 347/640 (54%)
9E7-35 / 169 / Phosphoenolpyruvate synthase/pyruvate phosphate dikinase / 770 / NP_613539 / Methanopyrus kandleri AV19 / 5e-19 / 73/216 (33%) / 101/216 (46%)
9E7-36 / 309 / Putative manganese transport system permease protein / 287 / CAE80910 / Bdellovibrio bacteriovorus HD100 / 4e-13 / 69/229 (30%) / 115/229 (50%)
9E7-37 / 410 / Hydroxymethylglutaryl-CoA reductase / 419 / BAC96651 / Vibrio vulnificus YJ016 / 6e-109 / 213/368 (57%) / 269/368 (73%)
9E7-38 / 120 / Unknown
9E7-39 / >203 / Short-chain dehydrogenase/reductase SDR / 254 / ZP_01103692 / gamma Proteobacterium KT 71 / 3e-34 / 77/175 (44%) / 112/175 (64%)

TableS2. Putative ORFs on cosmid clone21G8 and their information (E value0.01)

ORF / Homologous protein blast search information
Name / Length
(aa) / Homologous Protein / length (aa) / accession No. / Source / E-value / Identity(aa%) /
positive (aa%)
21G8-1 / >104 / Hypothetical protein MED297_00645
conserved hypothetical drug transport transmembrane protein / 401
393 / EAR08151
ABD82736 / Reinekea sp. MED297
Saccharophagus degradans 2-40 / 3e-18
3e-17 / 39/67 (58%) / 52/67 (77%)
40/67 (59%) / 51/67 (76%)
21G8-2 / 149 / Unknown
21G8-3 / 460 / Putative oxidoreductase / 452 / CAE31970 / Bordetella bronchiseptica RB50 / 6e-64 / 157/436 (36%) / 224/436 (51%)
21G8-4 / 220 / Unknown
21G8-5 / 460 / Alkane-1 monooxygenase / 401 / CAB54050 / Pseudomonas putida / 3e-120 / 214/377 (56%) / 274/377 (72%)
21G8-6 / 112 / Hypothetical protein / 153 / CAD84302 / Nitrosomonas europaea / 4e-07 / 34/90 (37%) / 47/90 (52%)
21G8-7 / 960 / 2-oxoglutarate dehydrogenase complex / 935 / ZP_00139211 / Pseudomonas aeruginosa / 0.0 / 507/894 (56%) / 642/894 (71%)
21G8-8 / 398 / 2-oxoglutarate dehydrogenase, E2 component, dihydrolipoamide succinyltransferase / 381 / AAU92043 / Methylococcus capsulatus str. Bath / 1e-122 / 233/397 (58%) / 299/397 (75%)
21G8-9 / 476 / 2-oxoglutarate dehydrogenase, E3 component, lipoamide dehydrogenase / 478 / AAO55717 / Pseudomonas syringae pv. tomato str. DC3000 / 5e-144 / 278/475 (58%) / 359/475 (75%)
21G8-10 / 132 / Arylsulfatase A and related enzymes / 536 / ZP_00262854 / Pseudomonas fluorescens PfO-1 / 2e-33 / 65/95 (68%) / 79/95 (83%)
21G8-11 / 162 / Unknown
21G8-12 / 236 / Unknown
21G8-13 / 150 / Hypothetical protein blr5132 / 193 / BAC50397 / Bradyrhizobium japonicum USDA 110 / 1e-30 / 72/151 (47%) / 103/151 (68%)
21G8-14 / 157 / Unknown
21G8-15 / 377 / Beta-lactamase class C and other penicillin binding proteins / 537 / ZP_00159285 / Anabaena variabilis / 2e-65 / 141/349 (40%) / 201/349 (57%)
21G8-16 / 307 / Transposase and inactivated derivatives, IS30 family / 248 / ZP_00203772 / Dechloromonas aromatica RCB / 2e-87 / 161/246 (65%) / 199/246 (80%)
21G8-17 / 614 / Predicted metal-dependent hydrolase with the TIM-barrel fold / 539 / ZP_00294260 / Thermobifida fusca / 1e-53 / 179/543 (32%) / 261/543 (48%)
21G8-18 / 71 / Unknown
21G8-19 / 113 / Unknown
21G8-20 / 72 / Uncharacterized conserved membrane protein / 66 / AAV82079 / Idiomarina loihiensis L2TR / 1e-06 / 25/63 (39%) / 40/63 (63%)
21G8-21 / 744 / Probable adenylate/guanylate cyclase protein / 634 / CAC47348 / Sinorhizobium meliloti / 7e-44 / 145/409 (35%) / 227/409 (55%)
21G8-22 / 549 / Hypothetical protein PJS6w01001636
TPR repeat / 532
537 / ZP_00364118
CAD85115 / Polaromonas sp. JS666
Nitrosomonas europaea / 3e-85
2e-81 / 195/507 (38%)/ 274/507 (54%)
195/528 (36%) / 275/528 (52%)
21G8-23 / 156 / Hypothetical protein / 186 / AAP58626 / 0.002 / 34/145 (23%) / 59/145 (40%)
21G8-24 / 254 / Unknown
21G8-25 / 161 / Unknown
21G8-26 / 74 / Unknown
21G8-27 / 501 / FAD/FMN-containing dehydrogenases / 479 / ZP_00194885 / Mesorhizobium sp. BNC1 / 6e-95 / 193/473 (40%) / 276/473 (58%)
21G8-28 / 174 / Unknown
21G8-29 / 139 / Hypothetical protein Desu02004052 / 136 / ZP_00103189 / Desulfitobacterium hafniense DCB-2 / 8e-04 / 35/132 (26%) / 58/132 (43%)
21G8-30 / 362 / Predicted hydrolases or acyltransferases
putative haloalkane dehalogenase / 282
303 / ZP_00213591
CAG19874 / Burkholderia cepacia
Photobacterium profundum SS9 / 7e-103
3e-30 / 184/278 (66%) / 212/278 (76%)
93/306 (30%) /154/306 (50%)