Table S1 List of GFLV isolates and accession numbers used in degenerate primer (corresponding to partial MPgene) design

Origin / Isolates / GenBank accessionnumber / Reference
France / GFLV-F13 / X16907 / SerghiniMA. et al.(1990)
B19a, A10a / AY780903, AY780902 / VigneE.et a.l(2005)
AB20b, AB13a, AB8a, AB4a, AB11a / EF426819, EF426813, EF426807, EF426805, EF426811 / VigneE.et al.(2009)
Germany / NP2, Sl2, Sl1A / AF438350, AF438354,AF438351 / WetzelT. et al.(2002)
Iran / S33-2, S32, Kh12-7 / FJ831671-3 / Sokhandan Bashir,N. et al.(2012)
X400-I1C3 / DQ286905 / Sokhandan Bashir,N. et al.(2007)
Italy / NE83, SG10 / DQ362929, DQ362923 / TerlizziF. at al.(2006)
Slovenia / Vol47c4, Vol51c3, Vol57c4, Vol55c3 / DQ922656, DQ922664, DQ92267,7 DQ922673 / Pompe-Novak,M et al.(2007)
USA / CAZINA4, CAZINA2, CAZINA1 / GU972574, GU972572, GU972571 / OliverJ.E et al.(2010)
WAME1492 / GQ332370 / MekuriaTA. et al.(2009)

Table S2 List of GFLV isolates and accession numbers used in degenerate primer (corresponding to partial CPgene) design

Origin / Isolates / GenBank accessionnumber / Reference
Africa / W5, W1, S2 / EU702443, EU702444, EU702441 / LiebenbergA(2009)
France / GFLV-F13 / X16907 / SerghiniMA(1990)
C8b, C8d / EF426835, EF426837 / VigneE(2009)
Iran / KH-12-2-3, KH-11-8-2, MG-28-1-3 / FJ513386, FJ513377, FJ513378 / Bashir(2011)
Takestan / JQ071377 / Nourinejhad ZarghaniS(2013)
Italy / SG16, SG12, SG11 / DQ362926, DQ362925, DQ362924 / TerlizziF(2006)
Slovenia / Vol54c2, Vol55c1, Vol52c1 / DQ922669, DQ922671, DQ922667 / Pompe-NovakM(2007)
USA / WAPN8133 / GQ332369 / MekuriaTA(2009)
CACSB4, CAZINA1 / GU972579, GU972571 / OliverJE(2010)
R6 / KC162001 / GottulaJ(2013)

Table S3 Primers used in RT-PCR assay for the detection of GFLV in grapevine

Primers / Sequence(5’- 3’)a / Location / Products size(bp) / References
GFLV-MP-F / ACCTTTCTCTATCAGRAHYCG / MP gene / 1760 / A.Liebenberg et al. (2009)
GFLV-NC-R / ACAAACAACACACTGTCGCC
GFLV V1/F / ACCGGATTGACGTGGGTGAT / CP gene / 311 / Sabche et al.(1991)
GFLV C1/R / CCAAAGTTGGTTTCCCAAGA
GFU / TCGGGTGAGACTGCGCAACTTCCTA / CP gene / 312 / Mackenzie et al.(1997)
GFD / GATGGTAACGCTCCCGCTGCTCTT
3’ primer P1 / GGGTGTATGTGGAAGAGGACG / CP gene / 722 / Y B Zhang et al.(2002)
5’ primer P2 / TTAAAGTCAGATACCATAGACTGG
CP 433V / GAACTGGCAAGCTGTCGTAGAAC / CP gene / 481 / N.S.Bashir et al.(2007)
CP 912C / GCTCATGTCTCTCTGACTTTGACC
FL-F1 / TAGGGCTGTTGGGGCTTT / HP gene / 219 / This study
FL-R1 / CGCTCATCACTAGTGTCATA
FL-F2 / CGGGGTGTATGTGGAAGAAG / CP gene / 299 / This study
FL-R2 / AACGAGAACCAATCTCA
FL-F3 / CAGACTGGCAAGCTGTCGT / CP gene / 390 / This study
FL-R3 / CCACATACACCCCGGGATAC
FL-F4 / TTTTGAGCAGGCCAGGAA / MP gene / 548 / This study
FL-R4 / CGTGTTGACGCAAAGTGCCAA
FL-F5 / GCGGCAGACTGGCAAGCTGT / CP gene / 515 / This study
FL-R5 / GGTCCTGCTATTGCATAAAAG
FL-F6 / GAGTCGAGAGCTGCGGCACT / HP gene / 569 / This study
FL-R6 / CCTTGCTCAATCATGTCG

a: R=A+G，Y=C+T，H=A+C+T;

Table S4 The results of detection of GFLV from different regions in China

Origin / Cultivar / DAS-ELISA / Primer pair
FL-MP1A/B / FL-MPn1A/B / FL-CP1A/B / FL-CPn1A/B
Anhui / Kyoho / - / - / - / - / -
Total（1）
Beijing / Chardonnay / - / - / - / - / -
Chardonnay / - / - / - / - / -
Chardonnay / - / - / - / - / -
Cabernet Franc / + / - / + / - / -
Cabernet Franc / - / - / - / - / -
Christmas Rose / - / - / - / - / -
Cabernet Sauvignon / - / - / - / - / -
Cabernet Sauvignon / - / - / - / - / -
Hongshuangwei / - / - / - / - / -
Hongshuangwei / - / - / - / - / -
MuscatHamburg / + / - / - / - / -
MuscatHamburg / - / - / - / - / -
Merlot / - / - / - / - / -
Total（13）
Gansu / Zuirenxiang / - / - / - / - / -
Total（1）
Guangxi / Kyoho / - / - / - / - / -
Total（1）
Hebei / Cabernet Sauvignon / - / - / - / - / -
Marselen / + / - / + / - / -
Marselen / - / - / - / -
Kyoho / - / - / - / - / -
Kyoho / - / - / - / - / -
Total（5）
Liaoning / Amber / + / - / - / - / -
Arman rose / - / - / - / - / -
Beda / + / - / - / - / -
Beda / - / - / - / - / -
Beda / - / - / - / - / -
Beda / - / - / - / - / -
Beda / - / - / - / - / -
Beda / - / - / - / - / -
Beda / - / - / - / - / -
Beda / - / - / - / - / -
Beda / - / - / - / - / -
Baiwashaka / + / - / - / - / +
Crimson Seedless / - / - / - / - / -
Guibao-1 / + / - / - / - / -
Guibao-2 / + / - / + / - / +
Halfords / + / - / - / - / -
Hongdan / + / - / + / - / +
Heihuxiang / + / - / + / - / +
Hongxinagfei / + / - / - / - / -
Jingzaojing / + / - / + / - / +
Kaiotome / - / - / + / - / -
Kilar / + / - / - / - / -
Keshenmaishen / + / - / + / - / +
Longan / + / - / + / - / -
Lizixiang / + / - / - / - / -
MuscatHamburg / + / - / - / - / -
Muscat Kyoho 1 / + / - / - / - / -
Muscat Kyoho 2 / + / - / - / - / +
Muscat Kyoho / - / - / - / - / -
Niuxin / + / - / + / - / +
Ora queen-1 / - / - / + / - / +
Ora queen-2 / + / - / + / - / +
Qiaobao-2 / + / - / + / - / +
Qiumi / + / - / + / - / -
Qiaqiwahe-1 / - / - / - / - / -
Qiaqiwahe-2 / + / - / + / - / +
Qiuwuhe / - / - / - / - / -
Red Fuji / + / - / + / - / +
Red Globe / - / - / - / - / -
Santos hani-1 / + / + / + / + / +
Santos hani-2 / + / + / + / + / +
Seneca / + / - / - / - / -
Sushanmeigui / + / - / + / - / +
Su-46 / + / - / - / - / -
Sy1 / - / - / - / - / -
Tamina / + / - / + / - / +
Uzbek rose / + / - / + / - / -
White Malaga-1 / + / - / - / - / -
White Malaga-2 / + / - / - / - / +
Yangbaimi / + / - / + / - / +
Yangbaimi / + / - / - / - / -
Zhengfu / + / - / - / - / -
Zhuosexiang / + / - / + / - / -
Zifeng / + / - / + / - / +
Zixiang Seedless / + / - / + / - / +
Zizhenzhu / + / - / - / - / -
102459 / + / - / - / - / -
228-1 / + / - / - / - / -
228-2 / + / - / + / - / +
426-1 / - / - / - / - / -
426-2 / - / - / - / - / -
87-1 / + / - / - / - / -
Total(62) / - / -
Ningxia / Chardonnay / + / - / - / - / -
Cabernet Sauvignon / - / - / - / - / -
Cabernet Sauvignon / - / - / - / - / -
Cabernet Sauvignon / - / - / - / - / -
Cabernet Sauvignon / - / - / - / - / -
Cabernet Gernischt / - / - / - / - / -
Cabernet Franc / - / - / - / - / -
Merlot / - / - / - / - / -
Total（8）
Shandong / Beibinghong / - / - / + / - / -
Beibinghong / - / - / - / - / -
Beibinghong / - / - / - / - / -
Beibinghong / - / - / - / - / -
Beibinghong / - / - / - / - / -
Beibinghong / - / - / - / - / -
Beibinghong / - / - / - / - / -
Beibinghong / - / - / - / - / -
Beibinghong / - / - / - / - / -
Beibinghong / - / - / - / - / -
Beibinghong / - / - / - / - / -
Beibinghong / - / - / - / - / -
Beibinghong / - / - / - / -
Beibinghong / - / - / - / - / -
Cabernet Sauvignon / - / - / - / - / -
Gamay / + / - / + / - / -
Italian Riesling / + / - / + / - / -
Italian Riesling / + / - / + / - / +
Italian Riesling / + / - / - / - / -
Italian Riesling / - / - / - / - / -
Italian Riesling / - / - / - / - / -
Italian Riesling / - / - / - / - / -
Italian Riesling / - / - / - / -
Italian Riesling / - / - / -
Italian Riesling / - / - / - / - / -
Italian Riesling / - / - / - / - / -
Italian Riesling / - / - / - / - / -
Italian Riesling / - / - / - / - / -
Italian Riesling / - / - / - / - / -
Italian Riesling / - / - / - / - / -
Italian Riesling / - / - / - / - / -
Italian Riesling / - / - / - / - / -
Ruby Seedless / - / - / - / - / -
Total（33）
Shanghai / Hupei-1 / - / - / - / - / -
Italy / - / - / - / - / -
Summer Black / - / - / - / - / -
Tamina / - / - / - / - / -
Total（4）
Sichuan / Red Globe / - / - / - / - / -
Total（1）
Tianjing / Cabernet Sauvignon / + / - / + / - / -
Cabernet Sauvignon / - / - / + / - / -
Cabernet Sauvignon / - / - / - / - / -
Itingian Riesling / + / - / + / - / -
Total（4）
Xinjiang / Bai Seedless / - / - / - / - / -
Crimson Seedless / + / - / - / - / -
Cabernet Sauvignon / - / - / + / - / -
Thompson Seedless / - / - / - / - / -
Thompson Seedless / - / - / - / - / -
Thompson Seedless / - / - / - / - / -
Red Globe / - / - / - / - / -
Zixiang Seedless / - / - / + / - / -
Total（8）
Yunnan / Crimson Seedless / - / - / - / - / -
Total（1）

Table S5Details of GFLV isolates generated and analyzed in this study

Isolate / Source（province /region） / Host cultivar / Genomic region / GenBank accessionnumber
LN-JFYN / Liaoning / Kaiotome / MP / KP995135
LN-SDHN1 / Liaoning / Santos hani / MP, CP / KP995136; KP995172
LN-QM / Liaoning / Qiumi / MP / KP995141
LN-HFS / Liaoning / Red Fuji / MP, CP / KP995142; KP995173
LN-LY / Liaoning / Longan / MP / KP995143
LN-YBM / Liaoning / Yangbaimi / MP / KP995144
LN-WMG / Liaoning / Uzbek rose / MP / KP995146
LN-HD / Liaonin / Hongdan / MP, CP / KP995147; KP995175
LN-ZSX / Liaoning / Zhuosexiang / MP / KP995148
LN-QB2 / Liaoning / Qiaobao-2 / MP, CP / KP995149; KP995150; KP995176
LN-QQWH / Liaoning / Qiaqiwahe / MP, CP / KP995151; KP995152; KP995177
LN-ZF / Liaoning / Zifeng / MP, CP / KP995153; KP995154; KP995178
LN-NX / Liaoning / Niuxin / MP, CP / KP995155; KP995179
LN-KSMS / Liaoning / Keshenmaishen / MP,CP / KP995156; KP995180
LN-BJML / Liaoning / White malaga / CP / KP995181
LN-DMN / Liaoning / Tamina / MP, CP / KP995157; KP995182
LN-ALHH1 / Liaoning / Ora queen / MP, CP / KP995158; KP995183
LN-JZJ / Liaoning / Jingzaojing / MP, CP / KP995159; KP995184
LN-SSMG / Liaoning / Sushanmeimi / MP, CP / KP995160; KP995185
LN-BWSK / Liaoning / Baiwashaka / CP / KP995186
LN-ZXWH / Liaoning / Zixiangwuhe / MP, CP / KP995161; KP995187
LN-ALHH2 / Liaoning / Ora queen / MP, CP / KP995162; KP995188
LN-SDHN2 / Liaoning / Santos hani / MP, CP / KP995163; KP995164; KP995165; KP995189
LN-GB / Liaoning / Guibao / MP, CP / KP995166; KP995190
LN-228 / Liaoning / 228 / MP, CP / KP995167; KP995191
LN-HFX / Liaoning / Heihuxiang / MP, CP / KP995168; KP995192
LN-JMG2 / Liaoning / Muscat Kyoho 2 / CP / KP995193
XJ-CXZ / Xinjiang / Cabernet Sauvignon / MP / KP995170;
XJ-ZXWH / Xinjiang / Zixiangwuhe / MP / KP995133;
SD-GRX1 / Shandong / Italian Riesling / MP / KP995134
SD-GRX2 / Shandong / Italian Riesling / MP, CP / KP995145; KP995174
SD-BBY / Shandong / Beibinghong / MP / KP995169
TJ-GRX / Tianjing / Itingian Riesling / MP / KP995139
TJ-CXZ1 / Tianjing / Cabernet Sauvignon / MP / KP995140
TJ-CXZ2 / Tianjing / Cabernet Sauvignon / MP / KP995171
BJ-PLZ / Beijing / Cabernet Franc / MP / KP995137
HB-MSL / Hebei / Marselen / MP / KP995138