Table S2 One hundred primer pair sequences used for amplification in initial screening tests of potential EST-SSR markers

Primer code / Unigene ID / Repeat Motif / Forward primer / Reverse primer / Expected Product Length (bp) / Amplified PCR products
S1 / KA304235 / (CGG)5 / GAGAACGTGTACATGGCGAA / GATCTTCGTTGCCTCGACTC / 226 / N
S2 / KA289564 / (CAG)5 / GGACTGCCACCATGAAAGAT / TGCAGCCAATGTAAAAGCTG / 230 / L
S3 / KA289625 / (AG)10 / TAGAATGTGCAAGCATTGGC / GCCTTTCCAACTCTGTCTGC / 191 / L
S4 / KA289693 / (AT)6 / TTGGATGGGTTGGATGAGAT / CCATACGCGAACATACGTCA / 219 / E
S5 / KA289739 / (ACA)8 / CATCTGAGAATGTTCCTGCAA / CTTCCATAGCTTTCCGCTTG / 252 / L
S6 / KA289762 / (AAG)5 / AGATGAGCCACCAGATTTGC / AAACTCCCCAGAAGGGAAAA / 136 / L
S7 / KA289813 / (CAC)5 / ACAACACCAATCGCAAATGA / ATGCGGCCATAAGTGTAAGG / 109 / N
S8 / KA289857 / (AAG)5 / GCCTGATGCCAACAAAAAGA / AAACACGGTATCGGAAGCAC / 302 / E
S9 / KA289863 / (CAA)5 / TGAATAGCAGCAGCAACACC / TGTCTGCCGCTAATCTTGTG / 238 / N
S10 / KA289881 / (AT)6 / GACATGCAGGTAATTTTTCCAA / GGGAATCAAGTTGTGGCAGT / 185 / L
S11 / KA289956 / (GGT)5 / TTTGGATTCAAATGTTAATGGC / AATGAATTGGTGGCTCTTGG / 101 / N
S12 / KA290104 / (TC)10 / ATACGGTGACGTTTCGTTCC / GTAGAGCTGCCCTTGACCTG / 282 / L
S13 / KA290265 / (TCG)5 / TAAGGGTTGGTGGTGGAGTC / TCTCTGAGATCCGAGGAAGC / 104 / N
S14 / KA290448 / (ACC)5 / GCAATGGCAATGTTGTTCTG / ACCTCACTGTGGGGAAACAG / 262 / N
S15 / KA290584 / (CT)7 / CGCTCAAGGTTCTCACATCA / TGCTGCAATTACACATCCAGA / 223 / N
S16 / KA290712 / (AG)12 / CAGTTGGGTCTGTCATGTGC / TTTTGCTGGAGGAAGCTTGT / 293 / E
S17 / KA290782 / (CA)6 / CCATTGCCATCTCTAGCAAA / CCAAGCCAAACCAAGATGAT / 174 / N
S18 / KA290884 / (CAG)5 / CCCCAAATGCAACATCTCTT / AGTTTCCTCCTCACATTCGC / 134 / E
S19 / KA290928 / (AT)7 / CACCACCAGTTGCACCAATA / CGTTATACCCTTGGTGTGGG / 215 / E
S20 / KA291018 / (AAT)5 / CGAGTCGAAGGCATTTATCA / GTGGCTAGAACCAGGCTGAG / 191 / E
S21 / KA291056 / (TCA)5 / TCTCCAACATCGTTCAGCAG / AATCCAAACACAGACCAGCC / 181 / E
S22 / KA291381 / (GAA)5 / ATCTTTGGCACTTGCATTCC / TCAAACCCTCGACTACCAGG / 206 / E
S23 / KA291382 / (TC)9 / GACCGATGAGGCAATGAGAT / GGAAACAGAGACAAGCAGAGAGA / 263 / E
S24 / KA291475 / (CT)10 / CTTTTTCACTCTCGTCCCGA / CCAACTTGTCGACCCAAACT / 173 / N
S25 / KA291488 / (GAA)5 / CCAAAAGCTAGGAAGACAAGG / AAGCACAACTAGCCCTCCAA / 222 / E
S26 / KA291571 / (ACA)5 / AACAAATTACAAGGGCAGCAA / AGGACCGCTTACAATCCCTT / 145 / E
S27 / KA291717 / (TGA)5 / GCACATTTCCAAAGCCATTT / TAACCCCATAAAGCTCACGC / 221 / N
S28 / KA291759 / (CCA)5 / CTAGCCGAGATCCATCAACC / GCGCTGAAGAGTTTGTCTCC / 115 / E
S29 / KA291807 / (GGT)6 / TCAAGCTGGCTTCTGTGAAA / TTCATTGTTTCCGCATCAAA / 166 / L
S30 / KA291862 / (TC)7 / CACCGACCAAGGAAACAACT / GACAAAAACCGAAGGCAAAA / 131 / E
S31 / KA291971 / (CT)6 / CTACGCCGCTGTTCTCTCTC / AAGATGATCTGGGGTTGCAG / 110 / N
S32 / KA292005 / (CAT)6 / GCTTTCGCCTCATCAGAATC / TCAGACGGATGAGGCTTTCT / 130 / E
S33 / KA292128 / (TC)12 / AGCGACTTCTCTTCTGCGAG / GCGAACATCAACACCAACAC / 183 / E
S34 / KA292346 / (AAG)15 / CTGAGGCTCATCAACGCATA / GCATCTGTACAGCTCCTCCC / 136 / E
S35 / KA292456 / (AGC)6 / TCTCCTTCAGCTGCCAAGTT / TTGCTGTCCTTGTGGAACTG / 294 / E
S36 / KA292554 / (TC)11 / TCCAAATTAGCTTGCTTGCC / TGCCCTCAAATCAGTGCATA / 180 / E
S37 / KA293043 / (GA)6 / TCCGAAGGAGTGGTTGAATC / ATGGCCTCCTCCAAATCTCT / 186 / E
S38 / KA293044 / (TTTC)5 / AACAGAGAGAACAGCGGTGG / ACCTTCCCAAAAATCAACCC / 296 / E
S39 / KA293089 / (GCT)5 / AGCCCACATCATTCTGAAGC / CAACCTCACCTCTGATGGGT / 270 / E
S40 / KA293119 / (ATA)6 / ATGGGGGTTCAAGTCATTCA / CGGGGTTACTTCCAGACAAA / 133 / E
S41 / KA293139 / (TGGT)5 / CCAAGCCAAACTCTCCAGTC / TGAATGGGGTCAAAGGGTTA / 110 / E
S42 / KA304228 / (TA)6 / GGTTCCATACAACTCAACCAAA / AACACCAACTGATTACCGCC / 216 / N
S43 / KA293270 / (TA)7 / TACAAAGGCATTCCACCACA / GAATCCCTGTAGTTGCTATGGG / 304 / S
S44 / KA293317 / (TC)16 / AACTAATTACTTCTTGCCAAGGG / AGAAGGGGGTTGCAAATTCT / 219 / E
S45 / KA293348 / (TCT)5 / GACGGTGCTGATTACTGGGT / ATCCGGAACACCCTTCTTTT / 260 / N
S46 / KA293474 / (AAG)5 / CGGGATAAGTAACCACAGCC / CACTTGGTGTCTCATGTGGG / 259 / N
S47 / KA293534 / (AT)6 / GGGTTAGGGTTTGATTTGTACG / TCTCCATCTCCATCACCACA / 189 / L
S48 / KA293596 / (TC)13 / GTGAATTTAGGCGAACCCAA / AGTTGGAGATTGGTTGCCAG / 211 / E
S49 / KA293715 / (CTC)5 / CTCTCCTCTTCCCCAAATCC / GAACGTCTCGAGGTTTTTGC / 263 / E
S50 / KA293786 / (CCT)6 / GATGATTGGTGGGAATTTGG / ATTTGGCAAGTCAAACCCAG / 196 / E
S51 / KA294145 / (CT)7 / TATAGCAAATTCATGCGCCA / TCCTGAGATGCAAGCGTATG / 268 / N
S52 / KA294185 / (AG)7 / CTGCAAGGAAAAGGAGATGC / GCTCTCACGATGCTCAAACC / 301 / S
S53 / KA294212 / (GGC)5 / TGTTGCTGAGACAAATTCGC / AATGGCGTCTGGGACATTAG / 217 / E
S54 / KA294250 / (CTT)5 / GCCTTGTCGAGGATCTTGAG / GAGGAGGAGAGGGAAGGTTG / 207 / E
S55 / KA294338 / (CT)8 / AACTCAGGACGAGGAGACCA / CAACTCTCCAATCCCTGCTC / 181 / L
S56 / KA294511 / (ACA)9 / GGCTGATGCTTCTGGCTTAC / AAACACCATTCCAGGTCCAA / 293 / E
S57 / KA294614 / (TC)9 / CCAAAGTTCTCGATCCTCCA / TAAAGACCGAGAGCAGCAGG / 107 / E
S58 / KA294804 / (TCG)5 / GAGGACGTGATTGACCCCTA / AACTTCCGCACAAATTCCAG / 244 / L
S59 / KA294900 / (TC)12 / CTCTCATCGACCTTGGCTTC / TGAGCATGGTTGTACGCTTC / 283 / E
S60 / KA295640 / (GAT)5 / GGCTGTTGTTTAGGGGTTCA / CATCAGAAGTCACAGCCAAA / 279 / E
S61 / KA295663 / (TCT)5 / TCACCTTTCTCTTCTTCCTCTTC / CATTTCTGGGGATGATCTCG / 304 / E
S62 / KA295686 / (AAG)5 / CAAGCAAGAAACACCAACGA / TGTGAGAGCCAATTCCTTCC / 254 / E
S63 / KA295695 / (AT)6 / AAGATGAGGATTACAATGCAAAA / TCACATTTTTGTAACAACTTGGAA / 275 / N
S64 / KA296017 / (AGC)5 / GATTCTGATCTGAGCGAGGC / GCGACGTTTTCTGTGAGACA / 171 / S
S65 / KA296076 / (TGG)5 / AAGAGAAGAGAAAAGAGAGAAAGGC / TAGACTTCGACTCACCCGCT / 213 / E
S66 / KA296128 / (AT)9 / TTGTTGGATGGTGCTAGCCT / GGCACCAATTACAAGCAACA / 224 / E
S67 / KA296397 / (AAT)5 / GGACGGCCAGCATAAATAGT / TCAAGAGACTTGCAAACTTACAGAA / 301 / N
S68 / KA296405 / (GA)7 / GCCTCCTCTAGCAACCTCAA / AGTAGACAACGCCATGGACC / 164 / E
S69 / KA296546 / (TC)7 / TCTCCTTCGCTCTCTCCAAA / CTTCTCAGGCAGTTCCTTGG / 261 / N
S70 / KA296546 / (CAA)5 / CAAGGAGCCCAGTGAAGAAG / TCGTTGGCATAGAATCCAGA / 211 / L
S71 / KA296642 / (AAC)7 / CGACTCTTGACTCCTCTCTGC / AGTCGGACTGGGAATAGGCT / 207 / E
S72 / KA296764 / (TA)6 / ACTGTTCACCACCACGATGA / TGCATCCTAGCAGATAGCAAA / 194 / E
S73 / KA296818 / (GA)6 / TCACTGTTCCACTGGTCTGG / CAAAAGATAATCGGCCCTGA / 271 / N
S74 / KA296852 / (TG)10 / GCTCCAAGGTGCATTGAGAT / AGCTTGTTGTCGACGGAGAT / 267 / N
S75 / KA296909 / (TC)6 / GGAGCCTTATGGCTATTCTCG / TCATGAAACTTCCCCTGTCC / 121 / E
S76 / KA296941 / (ATA)9 / GAGAAACAACAATAAAATGGAGGC / AACCAGACGTTTGGAGCAAC / 233 / E
S77 / KA296999 / (TCT)7 / TTGATAGCACCACAGCTTGC / CGTTTCGGTGGTTCTGTTTT / 182 / E
S78 / KA297326 / (TC)6 / GCTCCTGTTTCTCTTGCACC / TGGTAATAATGCATGCCCAA / 274 / L
S79 / KA297349 / (AG)6 / TGCCATTGCCATTACCATTA / AATGTGCAGGGTGTCAACAA / 122 / E
S80 / KA297356 / (ATG)9 / TATTCCCACCACTCCTGCTC / CGACGATGATTACGAGGACA / 210 / E
S81 / KA297398 / (GGT)6 / TGGGGATTTGGAGTTGAAAG / AGCCAATCGCCTTTACAAGA / 152 / E
S82 / KA297425 / (ATC)6 / CAAATGGAGCTCAGTGACGA / CCGAACCCAATAGCACAATC / 162 / N
S83 / KA297558 / (GC)6 / CCTTCATCTCTCCGAGTTGC / TCACCACCAGATTCACTTGC / 127 / E
S84 / KA297607 / (AG)11 / ATCCGCAAAGGAGGAATCTT / TCGAAGAGGTCAAGGTCGAG / 298 / N
S85 / KA297608 / (AG)6 / CAAACGGCGATACTGCTACA / TCCTAACTAGGGTTTATTCTATCTTCA / 278 / E
S86 / KA297755 / (CTT)5 / AAATCACCAACACCAGCACC / CAAACAGAACCCTAGCAGCC / 195 / L
S87 / KA297878 / (CAACAG)6 / GTATTGGGAGCGCAAGATCA / GAGTCTTGACGGAGTCGAGG / 133 / E
S88 / KA297916 / (TA)7 / CAGTCTTGCACTCGCAAAGT / TCGATCTTCTTCTCTACTGGTTCA / 194 / E
S89 / KA298009 / (GCT)6 / GCCCTTTGTGGTTAGGTTCA / TCATGCATCGAAATAGCCAG / 280 / L
S90 / KA298112 / (GAT)5 / CAAAAGGTGGAGGAGGGAAT / GCAAAATCTCCGCTTGTCTC / 109 / E
S91 / KA298195 / (AAAT)5 / TCCGATGCATCATCAACAAT / CACAAGGATCCATCCTAATCAA / 297 / L
S92 / KA298226 / (GTG)5 / TCCTTTTGAGCTTGACCTGG / CTCAACGTGACACCATGGAC / 196 / E
S93 / KA298249 / (CT)6 / GTGGTGAAAGATGGCTTGGT / CAGCTTGAAACCAGCATGAA / 102 / N
S94 / KA298395 / (GTG)6 / TTAGGGGAAGTGTTTGGCAC / TTCTTCACACCTCAACAGCG / 180 / L
S95 / KA298440 / (TGA)5 / CGTGTACATGCAATGGAAGC / TGGTGTCAGGTCCATCAAAA / 175 / L
S96 / KA298824 / (AC)10 / AGTGCCTTAGGACTCTCCTTG / GTCATGCCTCCTTGTGGAAT / 196 / E
S97 / KA298842 / (CAT)6 / TGGCCTTTGTTCTTCTGTCG / GCACATCTCCGGCATATTTT / 125 / E
S98 / KA299081 / (TC)9 / CTTTCACCACCACCCTTTGT / TCCTCAGCTCTCCCTCCATA / 110 / E
S99 / KA299087 / (CTT)7 / CGATATAAAGTGGCCCTCCA / TTTCAAGCAAGCATGACCAC / 234 / N
S100 / KA299150 / (CAC)10 / CAAGAGCTCCATGGGAATGT / AGGGTTGGGGAGTGAAGACT / 151 / E

E indicates amplified PCR products of the expected size. L and Sindicateamplified PCR productslarger and smaller than anticipated, respectively. N indicates no amplification.