Table S2: Oligonucleotide Primers Used in This Study

Table S2: Oligonucleotide primers used in this study

Name / Sequence (5’→3’) / Function
actABpFa / TCGTCGTCTAGAGCTTCAACGGAACTCATGG / Forward primer for actAB promoter cloning
actABpR / CTTCGCGGTACC GACACGTGCTCCTCATCGTA / Reverse primer for actAB promoter cloning
KOactIF / AGCGCGTGAGGGACCACGAGGCAGGGAGGGGAACACATGATTCCGGGGATCCGTCGACC / Forward primer for actI deletion by Redirect mutagenesis
KOactIR / GGGTGTGGACCACCCGGTGTTCTCCCGGCCGGCTCATGCTGTAGGCTGGAGCTGCTTC / Reverse primer for actI deletion by Redirect mutagenesis
KOactVI-1F / AGCCGGGTTCGAGTGGGCGCTGCCAGGCTCGTCGCCATGATTCCGGGGATCCGTCGACC / Forward primer for actVI-1 deletion by Redirect mutagenesis
KOactVI-1R / GGTCCCCGTACCGGTCGAACTGCACGGCACGCATCACTTTGTAGGCTGGAGCTGCTTC / Reverse primer for actVI-1 deletion by Redirect mutagenesis
KOactVA-6F / ACGCCGGAGAGCTGTTGAGGAGGAGCAACGATGGCTGAAATTCCGGGGATCCGTCGACC / Forward primer for actVA-6 deletion by Redirect mutagenesis
KOactVA-6R / AGGTCAGGACGGCAGGATCGCCCCCCGCGGAGTCATGAATGTAGGCTGGAGCTGCTTC / Reverse primer for actVA-6 deletion by Redirect mutagenesis
KOactVA-5F / GGCCAGGACCGGCTGGACCCCGAGGGACCCGGCATGAGCATTCCGGGGATCCGTCGACC / Forward primer for actVA-5 deletion by Redirect mutagenesis
KOactVA-5R / GCGACGAAGCCCACCCGGGGATCGTTCACTTCAGCCATCTGTAGGCTGGAGCTGCTTC / Reverse primer for actVA-5 deletion by Redirect mutagenesis
KOactRF / AACGGTGGCCCGTTCATGCCATACGATGAGGAGCACGTGATTCCGGGGATCCGTCGACC / Forward primer for actR deletion by Redirect mutagenesis
KOactRR / TGTTCATGACTCCGCGGGGGGCGATCCTGCCGTCCTGACTGTAGGCTGGAGCTGCTTC / Reverse primer for actR deletion by Redirect mutagenesis
AR-TP1F / CACCGTGTCGCGAAGCGAGGAAGGGAGGC / Forward primer for cloning point mutated actRs in pET151
AR-TP1R / TCATGACTCCGCGGGGGGCGATCC / Reverse primer for cloning point mutated actRs in pET151
AR-V90WRb / CGGGACCTCCCACTCGGTCAGCTCG / Reverse internal mutagenic primer for actRV90W by megaprimer mutagenesis
AR-V92WR / CCGGTTCCGGCCACTCCACCTCGGTC / Reverse internal mutagenic primer for actRV92W by megaprimer mutagenesis
AR-V92YR / CCGGTTCCGGGTACTCCACCTCGGTC / Reverse internal mutagenic primer for actRV92Y by megaprimer mutagenesis
AR-M107WR / CGACCGGCACCACTCCTTGACCTGC / Reverse internal mutagenic primer for actRM107W by megaprimer mutagenesis
AR-L111WR / CATCCTCCGCCACGACCGGCACATC / Reverse internal mutagenic primer for actRL111W by megaprimer mutagenesis
AR-G135AF / GGACCCAACGCGATGGTGGGGATGG / Forward internal mutagenic primer for actRG135A by megaprimer mutagenesis
AR-G138AF / CGGGATGGTGGCGATGGAGCGCACC / Forward internal mutagenic primer for actRG138A by megaprimer mutagenesis
AR-G138FF / CGGGATGGTGTTCATGGAGCGCACC / Forward internal mutagenic primer for actRG138F by megaprimer mutagenesis
AR-G138LF / CGGGATGGTGCTGATGGAGCGCACC / Forward internal mutagenic primer for actRG138L by megaprimer mutagenesis
AR-G138WF / CGGGATGGTGTGGATGGAGCGCACC / Forward internal mutagenic primer for actRG138W by megaprimer mutagenesis
AR-G138YF / CGGGATGGTGTACATGGAGCGCACC / Forward internal mutagenic primer for actRG138Y by megaprimer mutagensis
AR-T142WF / GGATGGAGCGCTGGATGAACCTGCTGC / Forward internal mutagenic primer for actRT142W by megaprimer mutagenesis
AR-T142YF / GGATGGAGCGCTACATGAACCTGCTGC / Forward internal mutagenic primer for actRT142Y by megaprimer mutagenesis
AR-GTYWF / CGGGATGGTGTACATGGAGCGCTGGATGAACCTGCTGC / Forward internal mutagenic primer for actRGTYW by megaprimer mutagenesis
AR-GTWYF / CGGGATGGTGTGGATGGAGCGCTACATGAACCTGCTGC / Forward internal mutagenic primer for actRGTWY by megaprimer mutagenesis
AR-GTWWF / CGGGATGGTGTGGATGGAGCGCTGGATGAACCTGCTGC / Forward internal mutagenic primer for actRGTWWby megaprimer mutagenesis
AR-G160WF / CGCCTACGGGTGGGACCTGCTGTCG / Forward internal mutagenic primer for actRG160Wby megaprimer mutagenesis
AR-S164WF / CGACCTGCTGTGGACCTTCGTCACG / Forward internal mutagenic primer for actRS164W by megaprimer mutagenesis
AR-S164YF / CGACCTGCTGTACACCTTCGTCACG / Forward internal mutagenic primer for actRS164Y by megaprimer mutagenesis
AR-R225AF / CTCCGACCGCGCGTTCGAGCTCGGC / Forward internal mutagenic primer for actRR225A by megaprimer mutagenesis
TET-EMSA-F / CGTATCACGAGGCCCTTTCG / Forward primer for EMSA probe
TET-EMSA-R / TCATATTTGCCATCCATTTGC / Reverse primer for EMSA probe
AR/F / GGAGCACGGATCCGTGTCGCGAAGCGAGG / Forward primer for cloning wt or mutated actR into pMU1-actABplux
AR/R / CCGTGTTCTAGATCATGACTCCAGGCC / Reverse primer for cloning wt or mutated actR into pMU1-actABplux
actRFlk/F / ATTCCGTCTAGAGAGACGCCGGTGAA / Forward internal primer for cloning point mutated actR in pOJ260 by PCR driven overlapping extension
T142WRSD / GCGCAGCAGGTTCATCCAGCGCTCCATC / Reverse internal primer for cloning actRT142W in pOJ260 by PCR driven overlapping extension
T142WFSD / GTGGGGATGGAGCGCTGGATGAACCTGC / Forward internal primer for cloning actRT142W in pOJ260 by PCR driven overlapping extension
GTYWRSD / GCGCAGCAGCTTCATCCAGCGCTCCATGTACACCATC / Reverse internal primer for cloning actRGTYW in pOJ260 by PCR driven overlapping extension
GTYWFSD / CCCAACGGGATGGTGTACATGGAGCGCTGGATGAAC / Forward internal primer for cloning actRGTYW in pOJ260 by PCR driven overlapping extension
actRFlk/R / CGTCAGGAATTCCGTGGATCACT / Reverse primer for cloning point mutated actR in pOJ260 by PCR driven overlapping extension

a Restriction site for cloning is boldfaced.

b Point mutation in actR is underlined.