Table S1Details of experimental evidence provided in the reports demonstrating the beneficial effect of silicon amendment for the soybean cultivation
Sr. No. / Observed effect of silicon in soybean cultivation / referenceInsect resistance
1 / Resistance induced on against Bemisiatabaci / Ferreiraet al., 2011
Disease resistance
1 / Resistance to soybean rust / Lemeset al., 2011
2 / Resistance to soybean rust / Arsenault-Labrecqueet al., 2012
3 / Resistance to soybean rust / Rodrigues et al., 2009
4 / Resistance to soybean rust / Visseret al., 2011
Abiotic stress tolerance
1 / Salinity tolerance / Hamayunet al., 2010
2 / Salinity tolerance / Lee et al., 2010
3 / Elevate aluminium toxicity / Baylis 1994
4 / Drought tolerance / Hamayunet al., 2010
5 / Ultraviolet-B tolerance / Shenet al., 2010
6 / Decrease the Mn concentration in leaf / Kluthcouski and Nelson 1980
Agronomical performance
1 / Enhanced vegetative development / Ferreiraet al., 2011
2 / Enhanced soybean seedlings grown in potassium-deficient medium / Miao et al., 2010
3 / Growth promoting in nutrient solution / Miyake and Takahashi 1985
4 / Enhanced seed germination and seedlings growth rate / Li et al., 2004
References:
Arsenault-Labrecque G, Menzies JG, Bélanger RR.2012.Effect of Silicon Absorption on Soybean Resistance to Phakopsorapachyrhizi in Different Cultivars.Plant Disease96: 37-42.
Baylis AD, Gragopoulou C, Davidson KJ, Birchall JD. 1994.Effects of silicon on the toxicity of aluminium to soybean.Communications in Soil Science & Plant Analysis25: 537-546.
Ferreira RS, Moraes JC. 2011.Silicon influence on resistance induction against Bemisiatabaci biotype B (Genn.)(Hemiptera: Aleyrodidae) and on vegetative development in two soybean cultivars.Neotropical Entomology40: 495-500.
Hamayun, M, Sohn, EY, Khan SA, Shinwari ZK, Khan AL, LeeIJ. 2010. Silicon alleviates the adverse effects of salinity and drought stress on growth and endogenous plant growth hormones of soybean (Glycine Max L.). Pakisthan Journal of Botany42: 1713-1722.
Kluthcouski J, Nelson LE. 1980.The effect of silicon on the manganese nutrition of soybeans (Glycine max (L.) Merrill).Plant and Soil,56: 157-160.
Lee SK, Sohn EY, Hamayun M, Yoon JY, Lee IJ. 2010.Effect of silicon on growth and salinity stress of soybean plant grown under hydroponic system.Agroforestry systems80: 333-340.
Lemes EM, Mackowiak CL, Blount A, Marois JJ, Wright DL, Coelho L, Datnoff LE. 2011. Effects of silicon applications on soybean rust development under greenhouse and field conditions.Plant Disease95: 317-324.
Li Q, Ma C, Li H, Xiao Y, Liu X. 2004.Effects of soil available silicon on growth, development and physiological functions of soybean.Chinese Journal of Applied Ecology1: 015.
Miao BH, Han XG, ZhangWH. 2010.The ameliorative effect of silicon on soybean seedlings grown in potassium-deficient medium.Annals of Botany105: 967-973.
Miyake Y, Takahashi E. 1985.Effect of silicon on the growth of soybean plants in a solution culture.Soil science and plant nutrition31: 625-636.
Rodrigues FA, Duarte HS, Domiciano GP, Souza CA, Korndörfer GH, Zambolim L. 2009. Foliar application of potassium silicate reduces the intensity of soybean rust.Australasian Plant Pathology38: 366-372.
Shen X, Zhou Y, Duan L, Li Z, Eneji AE, Li J. 2010. Silicon effects on photosynthesis and antioxidant parameters of soybean seedlings under drought and ultraviolet-B radiation. Journal of plant physiology167: 1248-1252.
Visser D, Caldwell P, Laing M. 2011.The effect of potassium silicate on the control of Phakopsorapachyrhizi on soybean.Proceedings of The 5th International Conference on Silicon in Agriculturepp198.
Table S2 Details of 70 major intrinsic proteins (MIPs) used as query in BLAST search performed to identify MIPs in soybean genome
Sr. No. / Gene ID / NCBI Acc. No. / Protein length1. / AtNIP1-1 / AEE84127.1 / 296
2. / AtNIP1-2 / AEE84106.1 / 294
3. / AtNIP2-1 / CAC81707.2 / 288
4. / AtNIP3-1 / AEE31413.1 / 323
5. / AtNIP4-2 / AED94236.1 / 283
6. / AtNIP5-1 / AEE82874.1 / 304
7. / AtNIP6-1 / AEE36445.1 / 305
8. / AtNIP7-1 / AEE74343.1 / 275
9. / AtPIP1-1 / AEE80201.1 / 286
10. / AtPIP1-2 / NP001078066.1 / 274
11. / AtPIP1-3 / AEE27312.1 / 286
12. / AtPIP1-4 / Q39196.1 / 287
13. / AtPIP1-5 / AEE84748.1 / 287
14. / AtPIP2-1 / AEE79083.1 / 287
15. / AtPIP2-2 / AEC09362.1 / 285
16. / AtPIP2-3 / AEC09363.1 / 285
17. / AtPIP2-4 / Q9FF53.1 / 291
18. / AtPIP2-5 / Q9SV31.1 / 286
19. / AtPIP2-6 / Q9ZV07.1 / 289
20. / AtPIP2-7 / AEE86464.1 / 280
21. / AtPIP2-8_ / NP179277.1 / 280
22. / AtPIP3 AA / B36949.1 / 280
23. / AtSIP1-1 / AEE74036.1 / 240
24. / AtSIP1-2 / AED92534.1 / 243
25. / AtSIP2-1 / AEE79591.1 / 237
26. / AtTIP1-1 / AEC09303.1 / 251
27. / AtTIP1-2 / AEE77174.1 / 253
28. / AtTIP1-3 / AEE82031.1 / 252
29. / AtTIP2-1 / AEE75789.1 / 250
30. / AtTIP2-2 / AEE83878.1 / 250
31. / AtTIP2-3 / AED95514.1 / 250
32. / AtTIP3-1 / AEE35427.1 / 268
33. / AtTIP3-2_ / O22588.1 / 267
34. / AtTIP4-1_ / AEC07755.1 / 249
35. / AtTIP5-1_ / AEE78281.1 / 256
36. / LjXIP / CCI69207 / 299
37. / SlXIP / NP001238800 / 328
38. / OsNIP1_1_ / BAD27715.1 / 284
39. / OsNIP1_2_ / BAD73177.1 / 303
40. / OsNIP1_3_ / AAV44140.1 / 286
41. / OsNIP1_4_ / BAD53665.1 / 273
42. / OsNIP2_1_ / BAD16128.1 / 298
43. / OsNIP2_2_ / BAD37471.1 / 298
44. / OsNIP3_1_ / AAG13499.1 / 241
45. / OsNIP3_2_ / BAC99758.1 / 305
46. / OsNIP3_3_ / BAC65382.1 / 278
47. / OsNIP4_1_ / BAB61180.1 / 286
48. / OsPIP1_1_ / BAD28398.1 / 289
49. / OsPIP1_2_ / Q7XSQ9.3 / 288
50. / OsPIP1_3_ / BAD22920.1 / 288
51. / OsPIP2_1_ / BAC15868.1 / 290
52. / OsPIP2_2_ / BAD23735.1 / 288
53. / OsPIP2_3_ / CAD41442.1 / 290
54. / OsPIP2_4_ / BAC16113.1 / 286
55. / OsPIP2_5_ / BAC16116.1 / 283
56. / OsPIP2_6_ / CAE05002.2 / 282
57. / OsPIP2_7_ / BAD46581.1 / 290
58. / OsPIP2_8_ / AAP44741.1 / 280
59. / OsSIP1_1_ / BAB32914.1 / 177
60. / OsSIP2_1_ / NP001049950 / 250
61. / OsTIP1_1_ / AAK98737.1 / 250
62. / OsTIP1_2_ / BAB63833.1 / 252
63. / OsTIP2_1_ / BAD25765.1 / 248
64. / OsTIP2_2_ / BAD61899.1 / 248
65. / OsTIP3_1_ / AAG13544.1 / 264
66. / OsTIP3_2_ / CAE05657.2 / 265
67. / OsTIP4_1_ / AAS98488.1 / 251
68. / OsTIP4_2_ / BAA92993.1 / 251
69. / OsTIP4_3_ / BAA92991.1 / 251
70. / OsTIP5_1_ / NP001053493.1 / 269
Table S3 Details of primers used for the quantitative PCR analysis of GmNIP2-1 and GmNIP2-2 genes in roots of soybean plant grown with or without silicon amendment in hydroponic conditions
Sr. No. / Gene ID / Primer ID / Primer Sequence / Product Size1 / Actin / GmActin-F / GAGCTATGAATTGCCTGATGG / 118
GmActin-R / GCTTTCATGAATTCCAGTAGC
2 / GmNIP2-1 / GmNIP2-1F / GAAGGTGATGTTGATTTGGGCGGTG / 149
GmNIP2-1R / CATTTCACTATCCTCCATTTCATCCAAGG
3 / GmNIP2-2 / GmNIP2-2F / TCCAAGACAAGACATTCAAGGGTGTGG / 146
GmNIP2-2R / CATTTCACTATCCTCCATTTCATCCAAGG
Table S4 Details of results obtained through BLASTp search performed using 70 known MIPs against the amino acid sequences representing soybean proteome
Sr. No. / BLAST Hit / Query / E-value / Score (bits)1 / Glyma01g27970 / AtPIP1-2_(NP_001078066.1) / 8.00E-127 / 453
2 / Glyma01g41670 / AtTIP2-2_(AEE83878.1) / 5.00E-109 / 394
3 / Glyma01g42950 / AtPIP1-3_(AEE27312.1) / 1.00E-145 / 515
4 / Glyma02g07680 / AtSIP1-1_(AEE74036.1) / 2.00E-72 / 272
5 / Glyma02g08110 / AtPIP2-2_(AEC09362.1) / 1.00E-138 / 492
6 / Glyma02g08120 / AtPIP2-2_(AEC09362.1) / 4.00E-138 / 491
7 / Glyma02g10520 / AtTIP1-3_(AEE82031.1) / 4.00E-124 / 444
8 / Glyma02g15870 / AtNIP7-1_(AEE74343.1) / 3.00E-72 / 272
9 / Glyma02g41400 / AtNIP4-2_(AED94236.1) / 1.00E-82 / 306
10 / Glyma02g42220 / OsPIP1-2(Q7XSQ9.3) / 2.00E-99 / 362
11 / Glyma03g14150 / AtPIP1-3_(AEE27312.1) / 6.00E-144 / 510
12 / Glyma03g27340 / AtSIP2-1_(AEE79591.1) / 4.00E-78 / 291
13 / Glyma03g33800 / AtPIP2-1_(AEE79083.1) / 3.00E-125 / 448
14 / Glyma03g34310 / AtTIP1-1_(AEC09303.1) / 1.00E-117 / 422
15 / Glyma04g00450 / AtPIP2-7_(AEE86464.1) / 2.00E-142 / 505
16 / Glyma04g08830 / AtTIP4-1(AEC07755.1) / 6.00E-108 / 390
17 / Glyma05g29500 / AtNIP1-2_(AEE84106.1) / 3.00E-88 / 325
18 / Glyma05g29510 / AtNIP1-2_(AEE84106.1) / 4.00E-101 / 368
19 / Glyma05g37730 / OsPIP1-2(Q7XSQ9.3) / 5.00E-148 / 523
20 / Glyma06g00550 / AtPIP2-7_(AEE86464.1) / 4.00E-140 / 497
21 / Glyma06g08910 / AtTIP4-1(AEC07755.1) / 1.00E-107 / 389
22 / Glyma06g46340 / AtSIP1-1_(AEE74036.1) / 8.00E-54 / 210
23 / Glyma07g02060 / AtTIP2-1_(AEE75789.1) / 1.00E-113 / 409
24 / Glyma07g02760 / AtNIP6-1_(AEE36445.1) / 4.00E-68 / 258
25 / Glyma07g02800 / AtNIP6-1_(AEE36445.1) / 1.00E-57 / 224
26 / Glyma07g03030 / AtNIP6-1_(AEE36445.1) / 2.00E-62 / 239
27 / Glyma07g34150 / AtNIP4-2_(AED94236.1) / 5.00E-79 / 294
28 / Glyma08g01860 / OsPIP1-2(Q7XSQ9.3) / 2.00E-148 / 525
29 / Glyma08g12650 / AtNIP1-2_(AEE84106.1) / 2.00E-96 / 352
30 / Glyma08g12660 / AtNIP1-2_(AEE84106.1) / 2.00E-102 / 372
31 / Glyma08g21730 / AtTIP2-1_(AEE75789.1) / 1.00E-113 / 409
32 / Glyma08g23230 / AtNIP6-1_(AEE36445.1) / 2.00E-121 / 436
33 / Glyma09g28930 / AtTIP3-1_(AEE35427.1) / 3.00E-105 / 381
34 / Glyma09g35860 / AtTIP5-1(AEE78281.1) / 4.00E-82 / 304
35 / Glyma09g37280 / OsNIP2-1(BAD16128.1) / 6.00E-74 / 278
36 / Glyma10g03870 / AtNIP7-1_(AEE74343.1) / 1.00E-70 / 266
37 / Glyma10g31750 / AtTIP3-1_(AEE35427.1) / 2.00E-103 / 375
38 / Glyma10g35520 / AtPIP2-2_(AEC09362.1) / 2.00E-139 / 495
39 / Glyma10g36560 / AtNIP5-1_(AEE82874.1) / 8.00E-119 / 427
40 / Glyma10g43680 / AtTIP1-3_(AEE82031.1) / 4.00E-118 / 424
41 / Glyma11g02530 / AtPIP1-3_(AEE27312.1) / 2.00E-144 / 512
42 / Glyma11g03690 / AtTIP2-2_(AEE83878.1) / 4.00E-110 / 397
43 / Glyma11g10350 / LjXIP_(CCI69207.1) / 2.00E-72 / 273
44 / Glyma11g10360 / LjXIP _(CCI69207.1) / 4.00E-106 / 384
45 / Glyma11g15200 / AtTIP1-3_(AEE82031.1) / 3.00E-111 / 401
46 / Glyma11g20600 / OsPIP2-2(BAD23735.1) / 7.00E-137 / 486
47 / Glyma11g35030 / OsPIP1-2(Q7XSQ9.3) / 4.00E-145 / 514
48 / Glyma12g01490 / AtTIP5-1(AEE78281.1) / 9.00E-45 / 180
49 / Glyma12g02640 / LjXIP _(CCI69207.1) / 3.00E-113 / 408
50 / Glyma12g02650 / LjXIP _(CCI69207.1) / 8.00E-71 / 267
51 / Glyma12g07120 / AtTIP1-1_(AEC09303.1) / 4.00E-106 / 384
52 / Glyma12g08040 / OsPIP2-2(BAD23735.1) / 2.00E-136 / 485
53 / Glyma12g10430 / AtSIP1-1_(AEE74036.1) / 6.00E-54 / 211
54 / Glyma12g29510 / OsPIP2-2(BAD23735.1) / 6.00E-134 / 477
55 / Glyma13g01800 / OsNIP1-1(BAD27715.1) / 1.00E-29 / 130
56 / Glyma13g20940 / AtTIP1-1_(AEC09303.1) / 2.00E-92 / 339
57 / Glyma13g29690 / AtNIP1-2_(AEE84106.1) / 4.00E-102 / 371
58 / Glyma13g40100 / OsPIP2-2(BAD23735.1) / 2.00E-134 / 479
59 / Glyma13g40820 / AtTIP1-3_(AEE82031.1) / 1.00E-113 / 409
60 / Glyma13g43250 / AtTIP2-1_(AEE75789.1) / 4.00E-108 / 390
61 / Glyma14g06680 / AtPIP1-4_(Q39196.1) / 1.00E-140 / 499
62 / Glyma14g07560 / AtNIP4-2_(AED94236.1) / 9.00E-84 / 310
63 / Glyma14g35030 / OsNIP1-1(BAD27715.1) / 5.00E-55 / 214
64 / Glyma15g00620 / AtNIP6-1_(AEE36445.1) / 4.00E-126 / 451
65 / Glyma15g02090 / AtTIP2-1_(AEE75789.1) / 2.00E-109 / 395
66 / Glyma15g04630 / AtTIP1-3_(AEE82031.1) / 2.00E-64 / 246
67 / Glyma15g09370 / AtNIP1-2_(AEE84106.1) / 1.00E-100 / 366
68 / Glyma16g04800 / AtSIP1-1_(AEE74036.1) / 1.00E-79 / 296
69 / Glyma16g26720 / AtSIP1-1_(AEE74036.1) / 6.00E-73 / 274
70 / Glyma16g27130 / AtPIP2-2_(AEC09362.1) / 2.00E-138 / 491
71 / Glyma16g27140 / AtPIP2-2_(AEC09362.1) / 1.00E-138 / 493
72 / Glyma16g33530 / AtTIP3-1_(AEE35427.1) / 8.00E-106 / 383
73 / Glyma18g42630 / OsPIP1-2(Q7XSQ9.3) / 4.00E-144 / 511
74 / Glyma18g49410 / OsNIP2-1(BAD16128.1) / 1.00E-74 / 280
75 / Glyma18g52360 / AtTIP1-3_(AEE82031.1) / 8.00E-123 / 439
76 / Glyma19g04450 / AtTIP2-1_(AEE75789.1) / 6.00E-105 / 380
77 / Glyma19g28430 / AtSIP1-1_(AEE74036.1) / 9.00E-81 / 300
78 / Glyma19g30320 / AtSIP2-1_(AEE79591.1) / 2.00E-81 / 302
79 / Glyma19g36530 / AtPIP2-1_(AEE79083.1) / 2.00E-126 / 452
80 / Glyma19g37000 / AtTIP1-1_(AEC09303.1) / 1.00E-117 / 422
81 / Glyma20g01750 / AtNIP4-2_(AED94236.1) / 4.00E-35 / 149
82 / Glyma20g31040 / AtNIP5-1_(AEE82874.1) / 6.00E-95 / 347
83 / Glyma20g32000 / AtPIP2-5_(Q9SV31.1) / 6.00E-139 / 493
84 / Glyma20g35860 / AtTIP3-1_(AEE35427.1) / 2.00E-100 / 365
Table S5 Functional annotation of soybean major intrinsic protein performed using BLAST2GO annotation tool
Sr. No. / Gene_ID / Sequence Description / min E Value / min Sim / #GOs / InterPro1 / Glyma01g27970 / mip pip subfamily / 0.00 E+00 / 94.80 / 9 / IPR000425; IPR022357; IPR023271; PTHR19139:SF56 (PANTHER)
2 / Glyma01g41670 / aquaporin tip2-3 / 1.60 E-142 / 92.70 / 3 / IPR000425; IPR022357; IPR023271; PTHR19139:SF57 (PANTHER)
3 / Glyma01g42950 / probable aquaporin pip1-4-like / 0.00 E+00 / 96.50 / 3 / IPR000425; IPR022357; IPR023271; PTHR19139:SF56 (PANTHER)
4 / Glyma02g07680 / mip sip subfamily / 3.10 E-165 / 85.00 / 3 / IPR000425; IPR023271; PTHR19139:SF3 (PANTHER)
5 / Glyma02g08110 / aquaporin pip2-2-like / 0.00 E+00 / 97.00 / 3 / IPR000425; IPR022357; IPR023271; PTHR19139:SF27 (PANTHER)
6 / Glyma02g08120 / aquaporin pip2-2-like / 0.00 E+00 / 96.40 / 3 / IPR000425; IPR022357; IPR023271; PTHR19139:SF27 (PANTHER)
7 / Glyma02g10520 / aquaporin tip1-3-like / 5.40 E-175 / 93.00 / 5 / IPR000425; IPR022357; IPR023271; PTHR19139:SF55 (PANTHER)
8 / Glyma02g15870 / probable aquaporin nip7-1-like / 0.00 E+00 / 79.40 / 3 / IPR000425; IPR022357; IPR023271; PTHR19139:SF23 (PANTHER)
9 / Glyma02g41400 / aquaporin mip family nip subfamily / 2.60 E-150 / 89.80 / 3 / IPR000425; IPR022357; IPR023271; PTHR19139:SF22 (PANTHER)
10 / Glyma02g42220 / aquaporin protein pip11 / 8.50 E-145 / 92.85 / 5 / IPR000425; IPR022357; IPR023271; PTHR19139:SF56 (PANTHER)
11 / Glyma03g14150 / mip pip subfamily / 0.00 E+00 / 95.00 / 3 / IPR000425; IPR022357; IPR023271; PTHR19139:SF56 (PANTHER)
12 / Glyma03g27340 / mip sip subfamily / 6.50 E-153 / 87.60 / 3 / IPR000425; IPR023271; PTHR19139:SF3 (PANTHER)
13 / Glyma03g33800 / aquaporin pip2-1 / 0.00 E+00 / 89.50 / 3 / IPR000425; IPR022357; IPR023271; PTHR19139:SF27 (PANTHER)
14 / Glyma03g34310 / tonoplast intrinsic protein / 1.10 E-150 / 95.20 / 3 / IPR000425; IPR022357; IPR023271; PTHR19139:SF52 (PANTHER), tmhmm (TMHMM)
15 / Glyma04g00450 / aquaporin pip2-7 / 0.00 E+00 / 95.90 / 3 / IPR000425; IPR022357; IPR023271; PTHR19139:SF50 (PANTHER)
16 / Glyma04g08830 / tonoplast intrinsic / 4.70 E-132 / 91.20 / 6 / IPR000425; IPR022357; IPR023271; PTHR19139:SF51 (PANTHER), SignalP-NN(euk) (SIGNALP), tmhmm (TMHMM)
17 / Glyma05g29500 / aquaporin nip1-2 / 3.00 E-136 / 86.30 / 5 / IPR000425; IPR022357; IPR023271; PTHR19139:SF22 (PANTHER)
18 / Glyma05g29510 / aquaporin nip1-2 / 4.90 E-174 / 91.00 / 3 / IPR000425; IPR022357; IPR023271; PTHR19139:SF22 (PANTHER)
19 / Glyma05g37730 / aquaporin pip1-2 / 0.00 E+00 / 95.40 / 3 / IPR000425; IPR022357; IPR023271; PTHR19139:SF56 (PANTHER), tmhmm (TMHMM)
20 / Glyma06g00550 / aquaporin pip2-7 / 0.00 E+00 / 95.20 / 3 / IPR000425; IPR022357; IPR023271; PTHR19139:SF50 (PANTHER)
21 / Glyma06g08910 / tonoplast intrinsic / 2.00 E-140 / 91.10 / 6 / IPR000425; IPR022357; IPR023271; PTHR19139:SF51 (PANTHER)
22 / Glyma06g46340 / mip sip subfamily / 6.10 E-170 / 82.20 / 3 / IPR000425; IPR023271; PTHR19139:SF3 (PANTHER)
23 / Glyma07g02060 / aquaporin tip2-1 / 9.00 E-141 / 93.90 / 3 / IPR000425; IPR022357; IPR023271; PTHR19139:SF58 (PANTHER), SignalP-NN(euk) (SIGNALP), tmhmm (TMHMM)
24 / Glyma07g02760 / mip nip subfamily / 1.00 E-98 / 84.70 / 3 / IPR000425; IPR022357; IPR023271; PTHR19139:SF22 (PANTHER)
25 / Glyma07g02800 / mip nip subfamily / 6.30 E-119 / 81.50 / 3 / IPR000425; IPR023271; PTHR19139:SF22 (PANTHER)
26 / Glyma07g03030 / mip nip subfamily / 3.20 E-102 / 74.60 / 3 / IPR000425; IPR023271; PTHR19139:SF22 (PANTHER)
27 / Glyma07g34150 / mip nip subfamily / 0.00 E+00 / 76.50 / 3 / IPR000425; IPR022357; IPR023271; PTHR19139:SF22 (PANTHER)
28 / Glyma08g01860 / aquaporin pip1-2 / 0.00 E+00 / 94.90 / 3 / IPR000425; IPR022357; IPR023271; PTHR19139:SF56 (PANTHER), tmhmm (TMHMM)
29 / Glyma08g12650 / aquaporin nip1-2 / 0.00 E+00 / 89.30 / 5 / IPR000425; IPR022357; IPR023271; PTHR19139:SF22 (PANTHER)
30 / Glyma08g12660 / aquaporin nip1-2 / 2.00 E-178 / 90.70 / 3 / IPR000425; IPR022357; IPR023271; PTHR19139:SF22 (PANTHER)
31 / Glyma08g21730 / aquaporin tip2-1 / 4.00 E-139 / 93.60 / 3 / IPR000425; IPR022357; IPR023271; PTHR19139:SF58 (PANTHER)
32 / Glyma08g23230 / mip nip subfamily / 0.00 E+00 / 85.00 / 11 / IPR000425; IPR022357; IPR023271; PTHR19139:SF22 (PANTHER)
33 / Glyma09g28930 / probable aquaporin tip-type alpha-like / 7.10 E-170 / 92.10 / 4 / IPR000425; IPR022357; IPR023271; PTHR19139:SF30 (PANTHER), tmhmm (TMHMM)
34 / Glyma09g35860 / probable aquaporin tip5-1-like / 9.40 E-166 / 86.90 / 3 / IPR000425; IPR022357; IPR023271; PTHR19139:SF54 (PANTHER)
35 / Glyma09g37280 / aquaporin nip2-1 / 0.00 E+00 / 79.55 / 4 / IPR000425; IPR022357; IPR023271; PTHR19139:SF22 (PANTHER)
36 / Glyma10g03870 / probable aquaporin nip7-1-like / 0.00 E+00 / 81.00 / 3 / IPR000425; IPR022357; IPR023271; PTHR19139:SF23 (PANTHER)
37 / Glyma10g31750 / probable aquaporin tip-type alpha-like / 2.50 E-167 / 91.00 / 4 / IPR000425; IPR022357; IPR023271; PTHR19139:SF30 (PANTHER), tmhmm (TMHMM)
38 / Glyma10g35520 / aquaporin pip2-2-like isoform 1 / 0.00 E+00 / 95.90 / 3 / IPR000425; IPR022357; IPR023271; PTHR19139:SF27 (PANTHER)
39 / Glyma10g36560 / aquaporin nip3-1 / 0.00 E+00 / 85.80 / 3 / IPR000425; IPR022357; IPR023271; PTHR19139:SF22 (PANTHER)
40 / Glyma10g43680 / aquaporin tip1-3-like / 2.10 E-176 / 91.40 / 5 / IPR000425; IPR022357; IPR023271; PTHR19139:SF55 (PANTHER)
41 / Glyma11g02530 / probable aquaporin pip1-4-like / 0.00 E+00 / 96.40 / 3 / IPR000425; IPR022357; IPR023271; PTHR19139:SF56 (PANTHER)
42 / Glyma11g03690 / aquaporin tip2-3 / 8.70 E-143 / 93.30 / 3 / IPR000425; IPR022357; IPR023271; PTHR19139:SF57 (PANTHER)
43 / Glyma11g10350 / mip pip subfamily / 4.30 E-79 / 82.30 / 3 / IPR000425; IPR023271
44 / Glyma11g10360 / mip pip subfamily / 4.60 E-166 / 80.00 / 3 / IPR000425; IPR023271; PS51257 (PROFILE)
45 / Glyma11g15200 / aquaporin tip1-1 / 5.30 E-174 / 94.40 / 3 / IPR000425; IPR022357; IPR023271; PTHR19139:SF55 (PANTHER), SignalP-NN(euk) (SIGNALP), tmhmm (TMHMM)
46 / Glyma11g20600 / aquaporin pip2-7 / 0.00 E+00 / 93.10 / 4 / IPR000425; IPR022357; IPR023271; PTHR19139:SF27 (PANTHER), tmhmm (TMHMM)
47 / Glyma11g35030 / aquaporin protein pip11 / 0.00 E+00 / 94.60 / 5 / IPR000425; IPR022357; IPR023271; PTHR19139:SF56 (PANTHER)
48 / Glyma12g01490 / probable aquaporin tip5-1-like / 1.80 E-107 / 63.50 / 4 / IPR000425; IPR022357; IPR023271; PTHR19139:SF54 (PANTHER), tmhmm (TMHMM)
49 / Glyma12g02640 / mip pip subfamily / 0.00 E+00 / 82.10 / 3 / IPR000425; IPR023271
50 / Glyma12g02650 / mip pip subfamily / 7.00 E-114 / 83.30 / 3 / IPR000425; IPR023271; SignalP-NN(euk) (SIGNALP), tmhmm (TMHMM)
51 / Glyma12g07120 / aquaporin tip1-1 / 1.20 E-166 / 90.10 / 3 / IPR000425; IPR022357; IPR023271; PTHR19139:SF55 (PANTHER)
52 / Glyma12g08040 / aquaporin pip2-7 / 0.00 E+00 / 92.75 / 4 / IPR000425; IPR022357; IPR023271; PTHR19139:SF27 (PANTHER)
53 / Glyma12g10430 / mip sip subfamily / 2.80 E-169 / 81.50 / 3 / IPR000425; IPR023271; PTHR19139:SF3 (PANTHER)
54 / Glyma12g29510 / aquaporin pip2-7 / 0.00 E+00 / 93.55 / 4 / IPR000425; IPR022357; IPR023271; PTHR19139:SF27 (PANTHER)
55 / Glyma13g01800 / aquaporin nip1-2 / 2.90 E-85 / 52.65 / 1 / IPR000425; IPR023271; PTHR19139:SF22 (PANTHER)
56 / Glyma13g20940 / tonoplast intrinsic protein / 1.50 E-159 / 81.50 / 3 / IPR000425; IPR022357; IPR023271; PTHR19139:SF52 (PANTHER)
57 / Glyma13g29690 / aquaporin nip1-2 / 0.00 E+00 / 92.20 / 3 / IPR000425; IPR022357; IPR023271; PTHR19139:SF22 (PANTHER)
58 / Glyma13g40100 / aquaporin pip2-7 / 0.00 E+00 / 93.55 / 4 / IPR000425; IPR022357; IPR023271; PTHR19139:SF27 (PANTHER), tmhmm (TMHMM)
59 / Glyma13g40820 / aquaporin tip1-1 / 1.50 E-174 / 93.20 / 3 / IPR000425; IPR022357; IPR023271; PTHR19139:SF55 (PANTHER)
60 / Glyma13g43250 / aquaporin tip2-1-like / 2.80 E-143 / 94.30 / 3 / IPR000425; IPR022357; IPR023271; PTHR19139:SF58 (PANTHER), SignalP-NN(euk) (SIGNALP), tmhmm (TMHMM)
61 / Glyma14g06680 / probable aquaporin pip-type 7a-like / 0.00 E+00 / 95.10 / 5 / IPR000425; IPR022357; IPR023271; PTHR19139:SF56 (PANTHER)
62 / Glyma14g07560 / aquaporin mip family nip subfamily / 1.10 E-150 / 90.20 / 3 / IPR000425; IPR022357; IPR023271; PTHR19139:SF22 (PANTHER)
63 / Glyma14g35030 / aquaporin nip1-2 / 4.30 E-154 / 74.20 / 3 / IPR000425; IPR022357; IPR023271; PTHR19139:SF22 (PANTHER), SignalP-NN(euk) (SIGNALP), tmhmm (TMHMM)
64 / Glyma15g00620 / mip nip subfamily / 9.90 E-150 / 88.50 / 3 / IPR000425; IPR022357; IPR023271; PTHR19139:SF22 (PANTHER)
65 / Glyma15g02090 / aquaporin tip2-1-like / 7.90 E-148 / 94.20 / 3 / IPR000425; IPR022357; IPR023271; PTHR19139:SF58 (PANTHER)
66 / Glyma15g04630 / aquaporin tip1-1 / 4.20 E-97 / 94.40 / 3 / IPR000425; IPR022357; IPR023271; PTHR19139:SF55 (PANTHER), SignalP-NN(euk) (SIGNALP), tmhmm (TMHMM)
67 / Glyma15g09370 / aquaporin nip1-2 / 0.00 E+00 / 92.30 / 3 / IPR000425; IPR022357; IPR023271; PTHR19139:SF22 (PANTHER)
68 / Glyma16g04800 / mip sip subfamily / 6.10 E-127 / 87.20 / 3 / IPR000425; IPR023271; PTHR19139:SF3 (PANTHER)
69 / Glyma16g26720 / mip sip subfamily / 1.50 E-163 / 85.40 / 3 / IPR000425; IPR023271; PTHR19139:SF3 (PANTHER)
70 / Glyma16g27130 / aquaporin pip2-2-like / 0.00 E+00 / 97.00 / 3 / IPR000425; IPR022357; IPR023271; PTHR19139:SF27 (PANTHER)
71 / Glyma16g27140 / aquaporin pip2-2-like / 0.00 E+00 / 97.00 / 3 / IPR000425; IPR022357; IPR023271; PTHR19139:SF27 (PANTHER)
72 / Glyma16g33530 / probable aquaporin tip-type alpha-like / 4.80 E-147 / 92.40 / 4 / IPR000425; IPR022357; IPR023271; PTHR19139:SF30 (PANTHER), tmhmm (TMHMM)
Table S6 Details of transmembrane domains identified in soybean MIPs using TMHMM and SOSUI servers.
Gene ID / length / TMHMM / SOSUI / Max TMExpAA / First60 / TMH / prediction / TMH
Glyma01g27970 / 254 / 110.27 / 7.92 / 5 / Membrane / 5 / 5
Glyma01g41670 / 249 / 146.42 / 28.58 / 7 / Membrane / 6 / 7
Glyma01g42950 / 286 / 129.39 / 8.24 / 6 / Membrane / 6 / 6
Glyma02g07680 / 247 / 134.96 / 32.44 / 6 / Membrane / 6 / 6
Glyma02g08110 / 285 / 136.57 / 22.39 / 6 / Membrane / 5 / 6
Glyma02g08120 / 285 / 136.26 / 22.39 / 6 / Membrane / 5 / 6
Glyma02g10520 / 252 / 136.59 / 25.27 / 7 / Membrane / 6 / 7
Glyma02g15870 / 293 / 135.34 / 1.3 / 6 / Membrane / 6 / 6
Glyma02g41400 / 215 / 127.28 / 37.26 / 7 / Membrane / 6 / 7
Glyma02g42220 / 316 / 118.74 / 3.26 / 5 / Membrane / 6 / 6
Glyma03g14150 / 284 / 131.97 / 7.76 / 6 / Membrane / 6 / 6
Glyma03g27340 / 230 / 106.82 / 41.2 / 5 / Membrane / 5 / 5
Glyma03g33800 / 286 / 131.1 / 20.75 / 6 / Membrane / 6 / 6
Glyma03g34310 / 250 / 146.85 / 25.76 / 6 / Membrane / 6 / 6
Glyma04g00450 / 275 / 131.57 / 22.17 / 6 / Membrane / 6 / 6
Glyma04g08830 / 246 / 145.39 / 34.01 / 7 / Membrane / 6 / 7
Glyma05g29500 / 243 / 108.35 / 36.71 / 5 / Membrane / 5 / 5
Glyma05g29510 / 270 / 127.53 / 20.52 / 6 / Membrane / 6 / 6
Glyma05g37730 / 287 / 129.5 / 7.26 / 6 / Membrane / 6 / 6
Glyma06g00550 / 278 / 131.09 / 22.14 / 6 / Membrane / 6 / 6
Glyma06g08910 / 246 / 146.91 / 33.93 / 7 / Membrane / 6 / 7
Glyma06g46340 / 239 / 119.37 / 40.82 / 5 / Membrane / 4 / 5
Glyma07g02060 / 248 / 150.6 / 29.25 / 6 / Membrane / 6 / 6
Glyma07g02760 / 181 / 89.55 / 21.1 / 4 / Membrane / 4 / 4
Glyma07g02800 / 184 / 88.82 / 22.33 / 4 / Soluble / - / 4
Glyma07g03030 / 248 / 111.63 / 18.49 / 5 / Membrane / 5 / 5
Glyma07g34150 / 268 / 148.68 / 24.92 / 7 / Membrane / 7 / 7
Glyma08g01860 / 289 / 129.71 / 5.27 / 6 / Membrane / 6 / 6
Glyma08g12650 / 271 / 125.96 / 17.48 / 6 / Membrane / 6 / 6
Glyma08g12660 / 274 / 128.16 / 20.13 / 6 / Membrane / 6 / 6
Glyma08g21730 / 248 / 150.52 / 29.25 / 7 / Membrane / 6 / 7
Glyma08g23230 / 306 / 127 / 0.07 / 5 / Membrane / 6 / 6
Glyma09g28930 / 255 / 137.08 / 23.61 / 6 / Membrane / 6 / 6
Glyma09g35860 / 247 / 141.02 / 25.84 / 6 / Membrane / 7 / 7
Glyma09g37280 / 293 / 111.35 / 9.14 / 5 / Membrane / 6 / 6
Glyma10g03870 / 276 / 136.03 / 17.84 / 6 / Membrane / 6 / 6
Glyma10g31750 / 254 / 125.38 / 16.25 / 5 / Membrane / 6 / 6
Glyma10g35520 / 296 / 138.42 / 11.6 / 6 / Membrane / 5 / 6
Glyma10g36560 / 290 / 117.55 / 0 / 5 / Membrane / 6 / 6
Glyma10g43680 / 252 / 144.48 / 26.31 / 7 / Membrane / 6 / 7
Glyma11g02530 / 286 / 130.27 / 8.39 / 6 / Membrane / 6 / 6
Glyma11g03690 / 249 / 147.89 / 28.65 / 7 / Membrane / 6 / 7
Glyma11g10350 / 201 / 101.69 / 43.77 / 5 / Membrane / 5 / 5
Glyma11g10360 / 270 / 137.76 / 42.9 / 6 / Membrane / 6 / 6
Glyma11g15200 / 252 / 142.28 / 25.05 / 6 / Membrane / 6 / 6
Glyma11g20600 / 286 / 132.3 / 19.87 / 6 / Membrane / 5 / 6
Glyma11g35030 / 289 / 127.84 / 4.35 / 6 / Membrane / 6 / 6
Glyma12g01490 / 187 / 93.33 / 24.67 / 4 / Membrane / 4 / 4
Glyma12g02640 / 312 / 128.16 / 12.01 / 6 / Membrane / 6 / 6
Glyma12g02650 / 170 / 70.04 / 24.69 / 3 / Membrane / 5 / 5
Glyma12g07120 / 245 / 140.83 / 25.15 / 6 / Membrane / 7 / 7
Glyma12g08040 / 286 / 131.95 / 19.85 / 6 / Membrane / 5 / 6
Glyma12g10430 / 239 / 126.72 / 42.71 / 6 / Membrane / 6 / 6
Glyma12g29510 / 287 / 128.51 / 19.78 / 6 / Membrane / 6 / 6
Glyma13g01800 / 226 / 90.54 / 40.03 / 4 / Membrane / 5 / 5
Glyma13g20940 / 250 / 134.45 / 26.93 / 6 / Membrane / 6 / 6
Glyma13g29690 / 273 / 131.67 / 17.5 / 6 / Membrane / 6 / 6
Glyma13g40100 / 287 / 129.38 / 19.77 / 6 / Membrane / 6 / 6
Glyma13g40820 / 252 / 142.21 / 24.73 / 7 / Membrane / 6 / 7
Glyma13g43250 / 247 / 138.23 / 28.77 / 6 / Membrane / 6 / 6
Glyma14g06680 / 289 / 124.82 / 3.33 / 6 / Membrane / 6 / 6
Glyma14g07560 / 216 / 133.11 / 42.17 / 7 / Membrane / 6 / 7
Glyma14g35030 / 221 / 115.45 / 37.8 / 5 / Membrane / 6 / 6
Glyma15g00620 / 304 / 130.07 / 0.09 / 6 / Membrane / 6 / 6
Glyma15g02090 / 247 / 138.37 / 28.83 / 6 / Membrane / 6 / 6
Glyma15g04630 / 153 / 97.18 / 33.89 / 4 / Membrane / 4 / 4
Glyma15g09370 / 267 / 131.86 / 22.24 / 6 / Membrane / 6 / 6
Glyma16g04800 / 248 / 132.52 / 32.56 / 6 / Membrane / 6 / 6
Glyma16g26720 / 245 / 134.93 / 32.78 / 6 / Membrane / 6 / 6
Glyma16g27130 / 285 / 136.53 / 22.36 / 6 / Membrane / 5 / 6
Glyma16g27140 / 285 / 136.54 / 22.36 / 6 / Membrane / 5 / 6
Glyma16g33530 / 255 / 138.09 / 21.97 / 6 / Membrane / 6 / 6
Glyma18g42630 / 304 / 129.29 / 0 / 6 / Membrane / 5 / 6
Glyma18g49410 / 295 / 109.15 / 7.18 / 5 / Membrane / 6 / 6
Glyma18g52360 / 252 / 135.82 / 25.69 / 6 / Membrane / 6 / 6
Glyma19g04450 / 237 / 137.64 / 28.6 / 6 / Membrane / 6 / 6
Glyma19g28430 / 248 / 131.98 / 33.97 / 6 / Membrane / 6 / 6
Glyma19g30320 / 236 / 104.51 / 40.74 / 5 / Membrane / 5 / 5
Glyma19g36530 / 285 / 130.19 / 20.86 / 6 / Membrane / 6 / 6
Glyma19g37000 / 250 / 151.09 / 26.7 / 7 / Membrane / 6 / 7
Glyma20g01750 / 238 / 131.76 / 35.07 / 6 / Membrane / 6 / 6
Glyma20g31040 / 263 / 111.47 / 0.04 / 5 / Soluble / - / 5
Glyma20g32000 / 284 / 134.13 / 20.84 / 6 / Membrane / 5 / 6
Glyma20g35860 / 254 / 128.59 / 19.09 / 6 / Membrane / 6 / 6
ExpAA: The expected number of amino acids intransmembrane helices.
First60: The expected number of amino acids in transmembrane helices in the first 60 amino acids of the protein.
TMH:The number of predicted transmembrane helices.
MAX TMH: The maximum number among the TMH predicted by TMHMM and SOSUI
Table S7 Details of transmembrane domains identified using TMHMM and SOSUI servers in the 70 MIPs including rice, Arabidopsis, lotus and tomato.
Sr. No. / Gene ID / NCBI Acc. No. / ProteinLength / TMHMM / SOSUI / MAX TMH
ExpAA / First60 / TMH / prediction / TMH
AtNIP1-1 / AEE84127.1 / 296 / 130.88 / 3.76 / 6 / Membrane / 6 / 6
AtNIP1-2 / AEE84106.1 / 294 / 123.04 / 5.74 / 6 / Membrane / 6 / 6
AtNIP2-1 / CAC81707.2 / 288 / 110.88 / 10.22 / 5 / Membrane / 6 / 6
AtNIP3-1 / AEE31413.1 / 323 / 127.52 / 14.96 / 6 / Membrane / 6 / 6
AtNIP4-2 / AED94236.1 / 283 / 132.12 / 10.84 / 6 / Membrane / 6 / 6
AtNIP5-1 / AEE82874.1 / 304 / 120.77 / 0.8 / 5 / Membrane / 6 / 6
AtNIP6-1 / AEE36445.1 / 305 / 127.7 / 0.03 / 6 / Membrane / 6 / 6
AtNIP7-1 / AEE74343.1 / 275 / 143.19 / 13.4 / 6 / Membrane / 6 / 6
AtPIP1-1 / AEE80201.1 / 286 / 129.83 / 7.79 / 6 / Membrane / 6 / 6
AtPIP1-2 / NP001078066.1 / 274 / 108.17 / 8.66 / 5 / Membrane / 5 / 5
AtPIP1-3 / AEE27312.1 / 286 / 130.86 / 7.41 / 6 / Membrane / 6 / 6
AtPIP1-4 / Q39196.1 / 287 / 130.83 / 6.43 / 6 / Membrane / 6 / 6
AtPIP1-5 / AEE84748.1 / 287 / 131.24 / 6.9 / 6 / Membrane / 5 / 6
AtPIP2-1 / AEE79083.1 / 287 / 134.9 / 20.74 / 6 / Membrane / 5 / 6
AtPIP2-2 / AEC09362.1 / 285 / 137.58 / 22.38 / 6 / Membrane / 5 / 6
AtPIP2-3 / AEC09363.1 / 285 / 138.22 / 22.2 / 6 / Membrane / 5 / 6
AtPIP2-4 / Q9FF53.1 / 291 / 133.06 / 21.09 / 6 / Membrane / 5 / 6
AtPIP2-5 / Q9SV31.1 / 286 / 135.64 / 21.76 / 6 / Membrane / 6 / 6
AtPIP2-6 / Q9ZV07.1 / 289 / 141.12 / 21.89 / 6 / Membrane / 5 / 6
AtPIP2-7 / AEE86464.1 / 280 / 131.52 / 22 / 6 / Membrane / 4 / 6
AtPIP2-8_ / NP179277.1 / 280 / 131.52 / 22 / 6 / Membrane / 5 / 6
AtPIP3 AA / B36949.1 / 280 / 132.61 / 22.08 / 6 / Membrane / 4 / 6
AtSIP1-1 / AEE74036.1 / 240 / 131.94 / 38.39 / 6 / Membrane / 6 / 6
AtSIP1-2 / AED92534.1 / 243 / 122.5 / 39.19 / 5 / Membrane / 5 / 5
AtSIP2-1 / AEE79591.1 / 237 / 121.28 / 38.93 / 6 / Membrane / 5 / 6
AtTIP1-1 / AEC09303.1 / 251 / 154.17 / 25.42 / 7 / Membrane / 6 / 7
AtTIP1-2 / AEE77174.1 / 253 / 147.62 / 25.42 / 7 / Membrane / 6 / 7
AtTIP1-3 / AEE82031.1 / 252 / 143.36 / 25.97 / 7 / Membrane / 6 / 7
AtTIP2-1 / AEE75789.1 / 250 / 148.24 / 28.92 / 6 / Membrane / 6 / 6
AtTIP2-2 / AEE83878.1 / 250 / 142.43 / 28.27 / 6 / Membrane / 6 / 6
AtTIP2-3 / AED95514.1 / 250 / 142.63 / 28.19 / 6 / Membrane / 7 / 7
AtTIP3-1 / AEE35427.1 / 268 / 126.67 / 12.96 / 5 / Membrane / 6 / 6
AtTIP3-2_ / O22588.1 / 267 / 131.42 / 19.11 / 6 / Membrane / 6 / 6
AtTIP4-1_ / AEC07755.1 / 249 / 149.49 / 33.76 / 7 / Membrane / 6 / 7
AtTIP5-1_ / AEE78281.1 / 256 / 135.69 / 26.01 / 6 / Membrane / 7 / 7
LjXIP / CCI69207 / 299 / 132.13 / 9.67 / 6 / Membrane / 7 / 7
SlXIP / NP001238800 / 328 / 147.59 / 0 / 6 / Membrane / 6 / 6
OsNIP1_1_ / BAD27715.1 / 284 / 129.06 / 10.55 / 6 / Membrane / 6 / 6
OsNIP1_2_ / BAD73177.1 / 303 / 134.58 / 0 / 6 / Membrane / 6 / 6
OsNIP1_3_ / AAV44140.1 / 286 / 118.56 / 5.08 / 5 / Membrane / 6 / 6
OsNIP1_4_ / BAD53665.1 / 273 / 136.7 / 4.59 / 6 / Membrane / 6 / 6
OsNIP2_1_ / BAD16128.1 / 298 / 131.92 / 9.04 / 6 / Membrane / 6 / 6
OsNIP2_2_ / BAD37471.1 / 298 / 132.14 / 6.28 / 6 / Membrane / 6 / 6
OsNIP3_1_ / AAG13499.1 / 241 / 135.73 / 38.9 / 6 / Membrane / 6 / 6
OsNIP3_2_ / BAC99758.1 / 305 / 127.25 / 0.01 / 6 / Membrane / 6 / 6
OsNIP3_3_ / BAC65382.1 / 278 / 129.96 / 0 / 6 / Membrane / 6 / 6
OsNIP4_1_ / BAB61180.1 / 286 / 133.85 / 5.72 / 6 / Membrane / 6 / 6
OsPIP1_1_ / BAD28398.1 / 289 / 129.9 / 4.26 / 6 / Membrane / 6 / 6
OsPIP1_2_ / Q7XSQ9.3 / 288 / 128.03 / 5.42 / 6 / Membrane / 5 / 6
OsPIP1_3_ / BAD22920.1 / 288 / 127.31 / 5.37 / 6 / Membrane / 6 / 6
OsPIP2_1_ / BAC15868.1 / 290 / 138.44 / 17.97 / 6 / Membrane / 6 / 6
OsPIP2_2_ / BAD23735.1 / 288 / 134.46 / 18.84 / 6 / Membrane / 5 / 6
OsPIP2_3_ / CAD41442.1 / 290 / 131.9 / 17.89 / 6 / Membrane / 5 / 6
OsPIP2_4_ / BAC16113.1 / 286 / 144.65 / 20.81 / 7 / Membrane / 5 / 7
OsPIP2_5_ / BAC16116.1 / 283 / 135.24 / 22.23 / 6 / Membrane / 5 / 6
OsPIP2_6_ / CAE05002.2 / 282 / 135.34 / 21.56 / 6 / Membrane / 5 / 6
OsPIP2_7_ / BAD46581.1 / 290 / 136.82 / 16.2 / 6 / Membrane / 6 / 6
OsPIP2_8_ / AAP44741.1 / 280 / 129.04 / 20.27 / 6 / Membrane / 6 / 6
OsSIP1_1_ / BAB32914.1 / 177 / 71.95 / 40.8 / 3 / Membrane / 3 / 3
OsSIP2_1_ / NP001049950 / 250 / 120.71 / 27.96 / 6 / Membrane / 5 / 6
OsTIP1_1_ / AAK98737.1 / 250 / 144.99 / 27.08 / 6 / Membrane / 6 / 6
OsTIP1_2_ / BAB63833.1 / 252 / 154.68 / 26.4 / 7 / Membrane / 7 / 7
OsTIP2_1_ / BAD25765.1 / 248 / 137.64 / 29.18 / 6 / Membrane / 6 / 6
OsTIP2_2_ / BAD61899.1 / 248 / 141.92 / 26.86 / 6 / Membrane / 6 / 6
OsTIP3_1_ / AAG13544.1 / 264 / 132.43 / 20.96 / 6 / Membrane / 5 / 6
OsTIP3_2_ / CAE05657.2 / 265 / 127.16 / 19.87 / 6 / Membrane / 6 / 6
OsTIP4_1_ / AAS98488.1 / 251 / 134.42 / 27.65 / 6 / Membrane / 6 / 6
OsTIP4_2_ / BAA92993.1 / 251 / 135.96 / 29.99 / 6 / Membrane / 6 / 6
OsTIP4_3_ / BAA92991.1 / 251 / 132.73 / 29.19 / 6 / Membrane / 6 / 6
OsTIP5_1_ / NP001053493.1 / 269 / 143.95 / 28.71 / 6 / Soluble / 0 / 6
ExpAA: The expected number of amino acids intransmembrane helices.
First60: The expected number of amino acids in transmembrane helices in the first 60 amino acids of the protein.
TMH:The number of predicted transmembrane helices.
MAX TMH: The maximum number among the TMH predicted by TMHMM and SOSUI
TableS8Details of predicted sub-cellular location of soybean major intrinsic protein identified by using Wolfpsort, Cello and TargetP servers
Gene_ID / Wolfpsort1 / Cello2 / TargetP3NIP1-1 / Vacuole / plasma membrane / _
NIP1-2 / Vacuole / plasma membrane / _
NIP1-3 / Vacuole / plasma membrane / _
NIP1-4 / Extra / plasma membrane / Secretory
NIP1-5 / Vacuole / plasma membrane / _
NIP1-6 / plasma membrane / plasma membrane / _
NIP1-7 / Cytoplasm / plasma membrane / Secretory
NIP1-8 / plasma membrane / plasma membrane / _
NIP1-9 / Vacuole / plasma membrane / _
NIP1-10 / plasma membrane / plasma membrane / _
NIP1-11 / plasma membrane / plasma membrane / _
NIP1-12 / plasma membrane / plasma membrane / _
NIP2-1 / plasma membrane / plasma membrane / Chloroplast
NIP2-2 / plasma membrane / plasma membrane / Chloroplast
NIP3-1 / plasma membrane / plasma membrane / Chloroplast
NIP3-2 / plasma membrane / plasma membrane / Chloroplast
NIP3-3 / Cytoplasm / plasma membrane / _
NIP3-4 / plasma membrane / plasma membrane / _
NIP3-5 / plasma membrane / plasma membrane / _
NIP3-6 / plasma membrane / plasma membrane / Chloroplast
NIP4-1 / plasma membrane / plasma membrane / _
NIP4-2 / plasma membrane / plasma membrane / Secretory
PIP1-1 / plasma membrane / plasma membrane / _
PIP1-2 / plasma membrane / plasma membrane / _
PIP1-3 / plasma membrane / plasma membrane / _
PIP1-4 / plasma membrane / plasma membrane / _
PIP1-5 / plasma membrane / plasma membrane / _
PIP1-6 / plasma membrane / plasma membrane / _
PIP1-7 / plasma membrane / plasma membrane / _
PIP1-8 / plasma membrane / plasma membrane / _
PIP2-1 / plasma membrane / plasma membrane / _
PIP2-2 / plasma membrane / plasma membrane / _
PIP2-3 / plasma membrane / plasma membrane / _
PIP2-4 / plasma membrane / plasma membrane / _
PIP2-5 / plasma membrane / plasma membrane / _
PIP2-6 / plasma membrane / plasma membrane / _
PIP2-7 / plasma membrane / plasma membrane / _
PIP2-8 / plasma membrane / plasma membrane / _
PIP2-9 / plasma membrane / plasma membrane / _
PIP2-10 / plasma membrane / plasma membrane / _
PIP2-11 / plasma membrane / plasma membrane / _
PIP2-12 / plasma membrane / plasma membrane / _
PIP2-13 / plasma membrane / plasma membrane / _
PIP2-14 / plasma membrane / plasma membrane / _
SIP1-1 / Vacuole / plasma membrane / Secretory
SIP1-2 / Vacuole / plasma membrane / Secretory
SIP1-3 / plasma membrane / plasma membrane / Secretory
SIP1-4 / plasma membrane / plasma membrane / Secretory
SIP1-5 / plasma membrane / plasma membrane / _
SIP1-6 / plasma membrane / plasma membrane / Secretory
SIP2-1 / plasma membrane / plasma membrane / Secretory
SIP2-2 / Vacuole / plasma membrane / Secretory
TIP1-1 / Endoplasmic reticulum / plasma membrane / Mitochondrion
TIP1-2 / Cytoplasm / plasma membrane / _
TIP1-3 / Cytoplasm / plasma membrane / _
TIP1-4 / Vacuole / plasma membrane / _
TIP1-5 / Vacuole / plasma membrane / _
TIP1-6 / Vacuole / plasma membrane / _
TIP1-7 / plasma membrane / plasma membrane / Mitochondrion
TIP1-8 / Cytoplasm / plasma membrane / _
TIP1-9 / Cytoplasm / plasma membrane / _
TIP2-1 / plasma membrane / plasma membrane / Secretory
TIP2-2 / Cytoplasm / plasma membrane / Secretory
TIP2-3 / plasma membrane / plasma membrane / Secretory
TIP2-4 / Vacuole / plasma membrane / _
TIP2-5 / Vacuole / plasma membrane / _
TIP2-6 / plasma membrane / plasma membrane / Secretory
TIP2-7 / plasma membrane / plasma membrane / Secretory
TIP3-1 / Cytoplasm / plasma membrane / _
TIP3-2 / Cytoplasm / plasma membrane / _
TIP3-3 / Mitochondrion / plasma membrane / _
TIP3-4 / Cytoplasm / plasma membrane / _
TIP4-1 / Cytoplasm / plasma membrane / _
TIP4-2 / Vacuole / plasma membrane / _
TIP5-1 / Chloroplast / plasma membrane / _
XIP1-1 / Cytoplasm / plasma membrane / _
XIP2-1 / Extra / plasma membrane / _
1 2 3
TableS9 Details of serine phosphorylation sites detected in major intrinsic proteins of soybean. The Z score was estimated by an artificial neural network method using NetPhos 2.0 Server phosphorylation prediction server (
Gene_ID / Protein Size / Phosphorylation sites / Serine Position / Sequence / Z scorePIP1-1 / 284 / 6 / 46 / GELSSWSFY / 0.641
79 / FKSKSKCST / 0.914
127 / ARKLSLTRA / 0.928
193 / YTVFSATDA / 0.977
204 / NARDSHVPI / 0.993
238 / NPARSLGAA / 0.925
282 / AIPFSSK-- / 0.905
PIP1-2 / 287 / 8 / 49 / RELTSWSFY / 0.932
80 / GVAKSPSKC / 0.927
82 / AKSPSKCST / 0.878
130 / ARKLSLTRT / 0.983
175 / SKGYSKGDG / 0.991
196 / YTVFSATDA / 0.977
207 / NARDSHVPI / 0.993
241 / NPARSLGAA / 0.928
PIP1-3 / 286 / 7 / 15 / AQKFSERQA / 0.827
26 / TGAKSDKDY / 0.998
46 / GELKSWSFY / 0.789
127 / ARKLSLTRA / 0.962
194 / YTVFSATDA / 0.977
205 / NARDSHVPI / 0.993
239 / NPARSLGAA / 0.925
PIP1-4 / 286 / 6 / 15 / AQKFSERQA / 0.827
46 / GELKSWSFY / 0.789
127 / ARKLSLTRA / 0.926
194 / YTVFSATDA / 0.977
205 / NARDSHVPI / 0.993
239 / NPARSLGAA / 0.925
PIP1-5 / 287 / 9 / 16 / ATKFSERQP / 0.871
47 / GELKSWSFY / 0.789
78 / GVNRSPSKC / 0.577
80 / NRSPSKCAS / 0.989
128 / ARKLSLTRA / 0.926
195 / YTVFSATDA / 0.977
206 / NARDSHVPI / 0.993
240 / NPARSLGAA / 0.925
PIP1-6 / 289 / 8 / 16 / ANKFSERQP / 0.692
49 / GELKSWSFY / 0.789
80 / GVNRSPSKC / 0.577
82 / NRSPSKCAS / 0.989
130 / ARKLSLTRA / 0.926
197 / YTVFSATDA / 0.977
208 / NARDSHVPI / 0.993
242 / NPARSLGAA / 0.925
PIP1-7 / 289 / 11 / 9 / EEDVSLGAN / 0.959
16 / ANKFSERQP / 0.692
27 / TAAQSQDDG / 0.995
50 / SELTSWSFY / 0.956
82 / VNRSSSKCA / 0.996
83 / NRSSSKCAT / 0.994
131 / ARKLSLTRA / 0.926
198 / YTVFSATDA / 0.977
205 / DAKRSARDS / 0.997
209 / SARDSHVPI / 0.992
243 / NPARSLGAA / 0.925
PIP1-8 / 289 / 9 / 9 / EQDVSLGAN / 0.868
27 / TAAQSQDDG / 0.995
50 / TEFTSWSFY / 0.829
83 / AGAKSKCST / 0.874
131 / ARKLSLPRA / 0.739
198 / YTVFSATDA / 0.977
205 / DAKRSARDS / 0.997
209 / SARDSHVPI / 0.992
243 / NPARSLGAA / 0.925
PIP2-1 / 285 / 9 / 10 / VEGGSFSAK / 0.537
12 / GGSFSAKDY / 0.994
62 / IGYKSQSDV / 0.985
64 / YKSQSDVKA / 0.603
119 / ARKVSLIRA / 0.915
185 / YTVFSATDP / 0.973
196 / NARDSHVPV / 0.983
230 / NPARSLGAA / 0.965
278 / KALGSFRSN / 0.653
PIP2-2 / 285 / 8 / 62 / IGYKSQSDV / 0.985
64 / YKSQSDVKA / 0.603
119 / ARKVSLIRA / 0.915
185 / YTVFSATDP / 0.973
196 / NARDSHVPV / 0.983
230 / NPARSLGAA / 0.965
271 / ILRASAAKA / 0.537
278 / KALGSFRSN / 0.653
PIP2-3 / 285 / 9 / 10 / VEGGSFSAK / 0.537
12 / GGSFSAKDY / 0.994
62 / IGYKSQSDV / 0.985
64 / YKSQSDVKA / 0.603
119 / ARKVSLIRA / 0.915
185 / YTVFSATDP / 0.973
196 / NARDSHVPV / 0.983
230 / NPARSLGAA / 0.965
278 / KALGSFRSN / 0.653
PIP2-4 / 285 / 10 / 10 / VEGGSFSAK / 0.537
12 / GGSFSAKDY / 0.994
62 / IGYKSQSDV / 0.985
64 / YKSQSDVKA / 0.603
119 / ARKVSLIRA / 0.915
185 / YTVFSATDP / 0.973
196 / NARDSHVPV / 0.983
230 / NPARSFGAA / 0.958
271 / ILRASAAKA / 0.736
278 / KAVGSFRSN / 0.777
PIP2-5 / 296 / 7 / 21 / AERGSFSGK / 0.916
23 / RGSFSGKDY / 0.997
130 / ARKVSLIRA / 0.915
196 / YTVFSATDP / 0.973
207 / NARDSHVPV / 0.983
241 / NPARSLGAA / 0.965
289 / KALGSFRSN / 0.653
PIP2-6 / 284 / 8 / 12 / AERGSFSGK / 0.916
14 / RGSFSGKDY / 0.997
118 / ARKVSLIRA / 0.915
157 / GGANSLADG / 0.919
184 / YTVFSATDP / 0.973
195 / NARDSHVPV / 0.983
229 / NPARSLGAA / 0.965
277 / KALGSFRSN / 0.653
PIP2-7 / 286 / 8 / 15 / GGEYSAKDY / 0.961
122 / GRKVSLIRA / 0.873
150 / GFQKSYYNR / 0.684
161 / GGANSVADG / 0.972
188 / YTVFSATDP / 0.973
199 / NARDSHVPV / 0.983
233 / NPARSFGAA / 0.958
281 / KALGSFRSN / 0.723
PIP2-8 / 286 / 7 / 15 / GGEYSAKDY / 0.961
122 / GRKVSLIRA / 0.873
161 / GGANSVADG / 0.972
188 / YTVFSATDP / 0.973
199 / NARDSHVPV / 0.983
233 / NPARSFGAA / 0.958
281 / KALGSFRSN / 0.723
PIP2-9 / 287 / 9 / 15 / QGEYSAKDY / 0.996
67 / YKRQSDTKI / 0.992
123 / GRKVSLVRA / 0.758
151 / GFQKSYYNR / 0.684
189 / YTVFSATDP / 0.973
196 / DPKRSARDS / 0.998
200 / SARDSHVPV / 0.982
234 / NPARSFGPA / 0.946
282 / KALGSFRSN / 0.724
PIP2-10 / 287 / 8 / 15 / QGEYSAKDY / 0.996
67 / YKRQSDTKI / 0.992
123 / GRKVSLVRA / 0.758
189 / YTVFSATDP / 0.973
200 / NARDSHVPV / 0.983
234 / NPARSFGPA / 0.946
275 / ILRGSAIKA / 0.66
282 / KALGSFRSN / 0.723
PIP2-11 / 286 / 7 / 36 / LRKWSFYRA / 0.932
120 / ARKVSLIRA / 0.956
148 / ALQKSYYNR / 0.788
186 / YTVFSATDP / 0.973
197 / VARDSHVPV / 0.965
231 / NPARSLGPA / 0.955
279 / KALGSFRSS / 0.597
PIP2-12 / 285 / 7 / 36 / LRKWSFFRA / 0.572
119 / ARKVSLTRA / 0.963
147 / ALQKSYYNR / 0.788
185 / YTVFSATDP / 0.973
196 / VARDSHVPV / 0.965
230 / NPARSLGPA / 0.955
278 / KALGSFRSS / 0.597
PIP2-13 / 275 / 9 / 6 / SKEVSQQRK / 0.96
109 / ARKVSLIRA / 0.913
141 / HSYNSLGGG / 0.881
148 / GGANSVSAG / 0.663
175 / YTVFSATDP / 0.973
182 / DPKRSARDS / 0.998
186 / SARDSHIPV / 0.989
220 / NPARSFGAA / 0.958
268 / KALGSFRSN / 0.869
PIP2-14 / 278 / 9 / 6 / SKEVSQEGL / 0.986
112 / ARKVSLIRA / 0.956
144 / HSYNSLGGG / 0.881
151 / GGANSVSAG / 0.663
178 / YTVFSATDP / 0.973
185 / DPKRSARDS / 0.998
189 / SARDSHVPV / 0.982
223 / NPARSLGAA / 0.965
271 / KALGSFRSN / 0.869
TIP1-1 / 250 / 8 / 15 / AQEASHRDT / 0.995
165 / VDPRSRRGS / 0.995
169 / SRRGSLGVM / 0.995
202 / NPAASFGPA / 0.843
211 / VVGWSWKNH / 0.926
239 / LIFVSHSRQ / 0.563
241 / FVSHSRQRF / 0.904
248 / RFRRSYY-- / 0.921
TIP1-4 / 245 / 5 / 62 / VVAASLSHA / 0.687
129 / TVGLSPGVG / 0.915
190 / FSGASMNPA / 0.6
196 / NPAVSFGPA / 0.865
236 / ITPNSYEQL / 0.971
TIP1-5 / 252 / 5 / 62 / VVAASLSHA / 0.687
134 / TSAFSLSPG / 0.945
136 / AFSLSPGVG / 0.886
203 / NPAVSFGPA / 0.865
243 / ITPNSYEHL / 0.963
TIP1-6 / 252 / 2 / 136 / AFALSPGVE / 0.872
203 / NPAVSFGPA / 0.865
TIP1-7 / 252 / 3 / 10 / IAIGSPREA / 0.995
134 / TSAFSLSSG / 0.966
248 / HEPLSNSDF / 0.987
TIP1-8 / 252 / 4 / 134 / TSGFSLSPG / 0.846
136 / GFSLSPGVS / 0.905
203 / NPAVSFGPA / 0.865
248 / HEPLSSSDF / 0.993
TIP1-9 / 252 / 3 / 136 / GFSLSPGVS / 0.965
203 / NPAVSFGPA / 0.865
248 / HEPLSSSDF / 0.993
TIP2-1 / 247 / 3 / 12 / SFNDSVSFA / 0.868
17 / VSFASIKAY / 0.89
200 / NPARSFGPA / 0.916
TIP2-2 / 247 / 2 / 17 / FSLASIKAY / 0.803
200 / NPARSFGPA / 0.916
TIP2-3 / 237 / 3 / 12 / NFNDSVSFA / 0.88
17 / VSFASIKAY / 0.89
200 / NPARSFGPA / 0.916
TIP2-4 / 248 / 6 / 12 / RFDDSFSLT / 0.581
14 / DDSFSLTSI / 0.743
17 / FSLTSIKAY / 0.903
135 / HSVASGVGA / 0.873
201 / NPARSFGPA / 0.916
245 / HAPLSSEF- / 0.88
TIP2-5 / 248 / 5 / 12 / RLDDSFSLT / 0.599
17 / FSLTSIKAY / 0.903
135 / HSVASGVGA / 0.873
201 / NPARSFGPA / 0.916
245 / HAPLSSEF- / 0.88
TIP2-6 / 249 / 2 / 126 / ITAKSIPSH / 0.51
200 / NPARSFGPA / 0.916
TIP2-7 / 249 / 4 / 12 / TLDDSFSAA / 0.806
17 / FSAASLKAY / 0.972
131 / IPSHSPANG / 0.99
200 / NPARSFGPA / 0.916
TIP3-1 / 254 / 5 / 15 / MNEASHPDS / 0.974
19 / SHPDSIRAA / 0.987
54 / EPGSSAGEL / 0.987
99 / GGRISVLRA / 0.559
170 / PKRGSIGSI / 0.978
TIP3-2 / 254 / 5 / 15 / MNEASHPDS / 0.974
19 / SHPDSIRAA / 0.987
54 / EPGSSAGEL / 0.987
99 / GGRISVLRA / 0.748
170 / PKRGSIGSI / 0.978
TIP3-3 / 255 / 5 / 7 / TRRYSFGRA / 0.997
19 / THPDSMRAT / 0.993
54 / DSAFSAGEL / 0.968
99 / GGRISVLRA / 0.977
170 / PKRGSVSNI / 0.992
TIP3-4 / 255 / 4 / 19 / THPDSMRAT / 0.993
54 / DSAFSAGEL / 0.968
99 / GGRISVLRA / 0.977
172 / RGAVSNIAP / 0.507
TIP4-1 / 246 / 3 / 8 / IALGSTREA / 0.99
191 / YSAASMNPA / 0.89
197 / NPARSFGPA / 0.834
TIP4-2 / 246 / 3 / 8 / IALGSTREA / 0.983
191 / YSAASMNPA / 0.89
197 / NPARSFGPA / 0.834
TIP5-1 / 247 / 11 / 15 / RFHESVTRN / 0.804
23 / NALRSYLSE / 0.686
146 / GFGASVLEG / 0.873
172 / RGPMSSTGI / 0.943
212 / AIAGSFRNQ / 0.55
NIP1-1 / 148 / 21 / MILPSESDL / 0.954
57 / TKYLSSTSH / 0.624
58 / KYLSSTSHL / 0.539
60 / LSSTSHLEA / 0.949
89 / DHRGSKDLT / 0.987
121 / NPARSLGPA / 0.862
NIP1-2 / 277 / 10 / 12 / NFDSSIKSE / 0.98
15 / SSIKSEFST / 0.968
18 / KSEFSTEQA / 0.993
32 / EAKHSPSNI / 0.933
141 / TKYLSSTSD / 0.514
142 / KYLSSTSDL / 0.993
144 / LSSTSDLEA / 0.926
173 / DHRGSKDLT / 0.987
205 / NPARSLGPA / 0.862
244 / EGRPSGGGI / 0.778
NIP1-3 / 268 / 4 / 147 / IPSGSYIQS / 0.852
202 / ISGASMNPA / 0.58
208 / NPARSLGPA / 0.862
255 / EIGASSKIF / 0.882
NIP1-4 / 238 / 3 / 117 / TPARSHIES / 0.995
178 / VSRVSMNPT / 0.988
184 / NPTRSLGPT / 0.786
NIP1-5 / 215 / 8 / 51 / LRRISGAHF / 0.867
70 / FRRFSYKEV / 0.998
134 / ISAVSTDDR / 0.981
166 / VSGASMNPA / 0.702
172 / NPARSIGPA / 0.914
212 / SIDKSSSE- / 0.991
213 / IDKSSSE-- / 0.952
214 / DKSSSE--- / 0.898
NIP1-6 / 311 / 12 / 14 / EEMPSMEEG / 0.995
20 / EEGVSSPSP / 0.526
21 / EGVSSPSPS / 0.989
23 / VSSPSPSRT / 0.997
25 / SPSPSRTCN / 0.966
47 / AQKVSDHDH / 0.943
134 / FRRFSYKQV / 0.997
198 / ISAVSTDDK / 0.985
262 / VSGASMNPA / 0.702
268 / NPARSIGPA / 0.914
304 / NFLRSPYKP / 0.958
310 / YKPPSE--- / 0.724
NIP1-7 / 243 / 5 / 82 / IAFASTRRF / 0.94
185 / NPVRSLGPA / 0.541
232 / EITKSASFL / 0.808
234 / TKSASFLKG / 0.86
242 / GRGGST--- / 0.569
NIP1-8 / 271 / 9 / 10 / AGTESQEVV / 0.555
22 / TKNTSETIQ / 0.72
30 / QRSDSLVSV / 0.986
106 / IAFASTRRF / 0.94
213 / NPARSLGPA / 0.929
255 / DKPLSEITK / 0.962
260 / EITKSASFL / 0.621
262 / TKSASFLKG / 0.86
270 / GRAASK--- / 0.884
NIP1-9 / 270 / 8 / 22 / NRDVSRTTQ / 0.971
30 / QASRSCVNV / 0.55
106 / IAFASTKRF / 0.981
138 / RLLFSGKEA / 0.967
213 / NPARSIGPA / 0.914
246 / WVYNSIRYT / 0.62
260 / EITKSASFL / 0.808
262 / TKSASFLKG / 0.715
NIP1-10 / 274 / 9 / 22 / NKDVSRTTQ / 0.898
30 / QPSRSCVNV / 0.66
74 / HPGISIVWG / 0.522
106 / IAFASTRRF / 0.94
138 / RLLFSGKET / 0.986
213 / NPARSIGPA / 0.914
260 / EITKSTSFL / 0.925
262 / TKSTSFLKG / 0.861
272 / GRSGSSR-- / 0.658
NIP1-11 / 273 / 6 / 31 / KCDDSANQD / 0.588
77 / QPGISIVWG / 0.793
153 / GTLPSGSDL / 0.846
216 / NPARSLGPA / 0.862
263 / EITKSASFL / 0.808
265 / TKSASFLKG / 0.628
NIP1-12 / 267 / 6 / 19 / NGDASKKCD / 0.992
25 / KCDDSSNQD / 0.691
26 / CDDSSNQDC / 0.792
71 / QPGISIVWG / 0.793
210 / NPARSLGPA / 0.862
257 / EITKSASFL / 0.808
NIP2-1 / 293 / 20 / 15 / AFIPSTIET / 0.923
21 / IETPSPSIP / 0.975
23 / TPSPSIPEI / 0.913
28 / IPEISSSSS / 0.759
30 / EISSSSSPG / 0.745
31 / ISSSSSPGS / 0.644
32 / SSSSSPGSL / 0.995
35 / SSPGSLARI / 0.858
72 / SAGLSKIDE / 0.996
80 / ERMVSKLGA / 0.901
112 / NPAVSLAFT / 0.595
151 / LLRPSNEIG / 0.889
158 / IGGTSPAGS / 0.77
162 / SPAGSHIQA / 0.952
231 / AIATSYYKG / 0.697
270 / PISLSSISS / 0.504
271 / ISLSSISSK / 0.501
273 / LSSISSKVR / 0.679
274 / SSISSKVRQ / 0.675
279 / KVRQSIGGT / 0.996
NIP2-2 / 295 / 19 / 15 / TFIPSTIET / 0.974
21 / IETPSPSIP / 0.975
23 / TPSPSIPEI / 0.913
29 / PEISSSSPS / 0.66
31 / ISSSSPSPG / 0.988
33 / SSSPSPGGS / 0.995
44 / RVAQSYPPG / 0.512
74 / SAGLSKIDE / 0.993
79 / KIDESMVSK / 0.593
114 / NPAVSLAFT / 0.595
153 / LLRPSDEIG / 0.975
160 / IGGTSPAGS / 0.77
164 / SPAGSHIQA / 0.952
233 / AIATSYYKG / 0.697
272 / PISLSSISS / 0.504
273 / ISLSSISSK / 0.501
275 / LSSISSKVR / 0.679
276 / SSISSKVRQ / 0.675
281 / KVRQSIGGT / 0.996
NIP3-1 / 304 / 7 / 33 / RNNNSKKSL / 0.888
36 / NSKKSLLKS / 0.671
40 / SLLKSCRCF / 0.772
45 / CRCFSVEEW / 0.996
50 / VEEWSLEDG / 0.995
298 / KAKTSISSF / 0.942
301 / TSISSFRR- / 0.905
NIP3-2 / 306 / 8 / 11 / PSLPSTSAT / 0.781
13 / LPSTSATPG / 0.604
40 / VVRKSSFLK / 0.83
41 / VRKSSFLKS / 0.744
45 / SFLKSCKCF / 0.921
65 / MPRVSCSLP / 0.675
165 / QVLASVSAA / 0.969
298 / KTPSSAPNG / 0.978
NIP3-3 / 248 / 5 / 16 / LNVASTKEW / 0.95
39 / LSSLSSKMI / 0.989
72 / KNDWSCYDD / 0.945
119 / QVLASVSAA / 0.799
154 / VAEFSVSFT / 0.605
NIP3-4 / 449 / 6 / 45 / QVLASVSAA / 0.799
61 / HPYMSGGVT / 0.766
227 / EKEKSQGSV / 0.944
287 / QVLASVSAA / 0.799
342 / TRVVSARVP / 0.972
363 / NRGGSHCHD / 0.93
NIP3-5 / 290 / 6 / 13 / PTAASVPAT / 0.943
33 / LRVDSLSHE / 0.983
35 / VDSLSHERE / 0.997
40 / HERESFSMA / 0.991
229 / SGPTSGGSM / 0.823
287 / RQVRSFRR- / 0.625
NIP3-6 / 263 / 6 / 13 / PTAASVPAT / 0.943
33 / LRVDSLSHE / 0.983
35 / VDSLSHERD / 0.997
40 / HERDSFAMA / 0.991
202 / SGPTSGGSM / 0.823
260 / RQVRSFRR- / 0.625
NIP4-1 / 340 / 13 / 11 / KHQSSDSSN / 0.95
14 / SSDSSNYAS / 0.837
18 / SNYASSSGL / 0.665
36 / RAATSKHRY / 0.915
149 / QTVGSMSAT / 0.799
202 / SESQSVGHL / 0.948
226 / TGPVSGGSM / 0.53
229 / VSGGSMNPA / 0.539
235 / NPARSLGPA / 0.862
281 / STLSSPNII / 0.632
298 / CSRRSGPMI / 0.944
312 / NWSLSYGRV / 0.561
331 / TKGISDGVY / 0.619
NIP4-2 / 282 / 10 / 91 / QTVGSMSAT / 0.799
140 / AALTSESQS / 0.766
144 / SESQSVGHL / 0.948
168 / TGPVSGGSM / 0.53
171 / VSGGSMNPA / 0.539
177 / NPARSLGPA / 0.862
223 / SILSSPNIS / 0.712
240 / CSRRSGPMI / 0.944
253 / KNWSSFSER / 0.764
273 / TKGISEGVY / 0.744
SIP1-3 / 248 / 2 / 132 / IGGPSLKVD / 0.804
239 / FPPPSPPEV / 0.886
SIP1-4 / 248 / 1 / 132 / IGGPSLKVD / 0.804
SIP1-5 / 239 / 4 / 76 / NPSTSLSFY / 0.644
88 / LRPDSSLSS / 0.945
91 / DSSLSSMAV / 0.561
134 / VDLHSGAVA / 0.64
SIP1-6 / 239 / 4 / 76 / NPSTSLSFY / 0.644
88 / LRPDSSLSS / 0.945
91 / DSSLSSMAV / 0.561
134 / VDLHSGAVA / 0.64
SIP2-1 / 230 / 1 / 127 / IHQGSLTEG / 0.942
SIP2-2 / 236 / 4 / 229 / KEDKSTSKS / 0.994
231 / DKSTSKSKS / 0.993
233 / STSKSKSD- / 0.997
235 / SKSKSD--- / 0.976
XIP1-1 / 312 / 6 / 6 / DDQFSTVHS / 0.619
10 / STVHSHSRE / 0.991
12 / VHSHSREPM / 0.996
25 / VHEKSSEPK / 0.761
143 / FVLKSVMEP / 0.947
167 / KGQSSGLRP / 0.746
XIP2-1 / 270 / 4 / 29 / ACLDSHEID / 0.763
62 / GGHMSPVFT / 0.923
160 / DKKRSRDLG / 0.666
256 / NTSVSKDHQ / 0.555