Table S1 Sequence Verification on the G237t Site in Wild Rice

Table S1Sequence verification on the g237t site in wild rice.

Species / Accession / Origin / g237t / Phenotype
O. rufipogon / YJCWR / Yunnan, China / G / shattering
O. rufipogon / B-2 / Yunnun, China / G / shattering
O. rufipogon / B-5 / Yunnun, China / G / shattering
O. rufipogon / B-10 / Yunnun, China / G / shattering
O. rufipogon / C-11 / Yunnun,
China / G / shattering
O. rufipogon / C-21 / Yunnun,
China / G / shattering
O. rufipogon / A-4 / Guangdong,China / G / shattering
O. rufipogon / C-15 / Guangdong, China / G / shattering
O. alta / IRRI-100161 / Brazil / G / shattering
O. latifolia / IRRI-100168 / Costa Rica / G / shattering
O. rufipogon / B-22 / Guangdong, China / G / shattering
O. rufipogon / S9049 / Guangdong, China / G / shattering
O. rufipogon / YD2-0225 / Guangxi, China / G / shattering
O. rufipogon / S0501 / Guangxi, China / G / shattering
O. rufipogon / S0502 / Guangxi, China / G / shattering
O. rufipogon / CL-1 / Hunan, China / G / shattering
O. rufipogon / CL-2 / Hunan, China / G / shattering
O. rufipogon / IRGC100204 / India / G / shattering
O. rufipogon / IRGC100189 / India / G / shattering
O. rufipogon / IRGC100208 / India / G / shattering
O. rufipogon / YD2-65 / Jiangxi, China / G / shattering
O. rufipogon / 94W-1 / Jiangxi, China / G / shattering
O. rufipogon / 94W-4 / Jiangxi, China / G / shattering
O. rufipogon / 94W-12 / Jiangxi, China / G / shattering
O. longistaminata / IRGC101199 / Cameroon / G / shattering

The p5gt and p3gt primers were used to amplify and sequence DNA fragments containing the g237t site from these wild rice alleles. *, The full length SHA1 cDNA YJCWR was sequenced.

Table S2 Sequence verification on the g237t site in domesticatedjaponica cultivars.

Accession / Origin / g237t / Phenotype
Handao 297 / China / T / non-shattering
Liangpishui handao / China / T / non-shattering
xiaobaimang / China / T / non-shattering
Changbaigu / China / T / non-shattering
Huangcungu / China / T / non-shattering
Anxin Putaohuang / China / T / non-shattering
Yelicanghua / China / T / non-shattering
Mirong / China / T / non-shattering
Buluodao / China / T / non-shattering
Xiaojingzi / China / T / non-shattering
Laoguangtou / China / T / non-shattering
Laohandao / China / T / non-shattering
Hongmang Handao / China / T / non-shattering
Dachangmang Handao / China / T / non-shattering
Xiaoluohan / China / T / non-shattering
Changzhong / China / T / non-shattering
Sanchaoqi / China / T / non-shattering
Zaoyedao / China / T / non-shattering
Niumiaohuang / China / T / non-shattering
Huangdao / China / T / non-shattering
Xueliqing / China / T / non-shattering
Putaolvzhong / China / T / non-shattering
Shidao / China / T / non-shattering
Muxiaoqiu / China / T / non-shattering
Jinlaotou / China / T / non-shattering
Sanyiqian / China / T / non-shattering
Baikeairihong / China / T / non-shattering
Tianjiqing / China / T / non-shattering
Chuheitouhong / China / T / non-shattering
Chenjianuo / China / T / non-shattering
Cunsanli / China / T / non-shattering
Ganshangchen / China / T / non-shattering
Fukuyama / Japan / T / non-shattering
Kahei / Japan / T / non-shattering
Tannkonnmochi / Japan / T / non-shattering
Gaoli Handao / Korea / T / non-shattering
Sin Heng Ho Yeng / Korea / T / non-shattering
Korye Chu / Korea / T / non-shattering
Zhonghua 15* / China / T / non-shattering
JX17* / China / T / non-shattering
Liangmangdao / China / T / non-shattering
Bayuemang / China / T / non-shattering
Baishami / China / T / non-shattering
Tiepazi / China / T / non-shattering
Mandao / China / T / non-shattering
Zaoshengfuguo / China / T / non-shattering
Modai / China / T / non-shattering
Hongguang / China / T / non-shattering
Min92-1 / China / T / non-shattering
Min101-1 / China / T / non-shattering
Mendaihuang / China / T / non-shattering
Changmaogu / China / T / non-shattering
Koshitomi / Japan / T / non-shattering
Akihikari / Japan / T / non-shattering
Nipponbare* / Japan / T / non-shattering
Minomasari / Japan / T / non-shattering
Kiyokaze / Japan / T / non-shattering
Hayakogane / Japan / T / non-shattering
Tomoyutaka / Japan / T / non-shattering
Shirakawa / Japan / T / non-shattering
Inachikara / Japan / T / non-shattering
Miyaganemochi / Japan / T / non-shattering
Sewon 392* / Korea / T / non-shattering
Shangzhou 11 / Korea / T / non-shattering
Gye Tong 400 / Korea / T / non-shattering
Pyeng Yang 10 / Korea / T / non-shattering
Huang Hae 33 / Korea / T / non-shattering
Pyeng Buk 5 / Korea / T / non-shattering
Pyang Buk 9 / Korea / T / non-shattering
Zek Kyeng / Korea / T / non-shattering
Mir Rib Zu / Korea / T / non-shattering
Sin Cen Zek Char / Korea / T / non-shattering
Menggudao / Mongolia / T / non-shattering
IRAT114 / Africa / T / non-shattering
Jabonl / Bangladesh / T / non-shattering
IRAT109 / Cote Divoire / T / non-shattering
ARC7042 / India / T / non-shattering
ARC10025 / India / T / non-shattering
ARC100376 / India / T / non-shattering
Abdey / India / T / non-shattering
Padi Bulu / Indonesia / T / non-shattering
Padi Bulu Gogo / Indonesia / T / non-shattering
Padi Pen Bulu / Indonesia / T / non-shattering
Padi Penda / Indonesia / T / non-shattering
Padi Tamput / Indonesia / T / non-shattering
Sagi / Indonesia / T / non-shattering
Sipapak / Indonesia / T / non-shattering
Djalawara / Indonesia / T / non-shattering
Dupanyui / Indonesia / T / non-shattering
Heritam / Indonesia / T / non-shattering
Dara Maman / Indonesia / T / non-shattering
IRAT13 / Ivory Coast (Africa) / T / non-shattering
Mack Kouk / Laos / T / non-shattering
Methi Noi / Laos / T / non-shattering
Do Khao / Laos / T / non-shattering
Kenteng / Laos / T / non-shattering
Lepsang / Laos / T / non-shattering
Belao / Malaysia / T / non-shattering
Rasi Pagalou / Malaysia / T / non-shattering
Padi Lasat / Malaysia / T / non-shattering
Libu / Philippines / T / non-shattering
Kutube / Philippines / T / non-shattering
Bunian / Philippines / T / non-shattering
Lepqu / Philippines / T / non-shattering
Kasinkan / Philippines / T / non-shattering
Agami M1 / Philippines / T / non-shattering
Lukip / Philippines / T / non-shattering
Balsamo / Philippines / T / non-shattering
Rathu Heenati / Sri Lanka / T / non-shattering
Chabli Chabol Glang / Thailand / T / non-shattering
Kao King Lang / Thailand / T / non-shattering
Neplun / Vietnam / T / non-shattering

The p5gt and p3gt primers were used to amplify and sequence DNA fragments containing the g237t site from these rice cultivars. *, The full length sha1 cDNA from Nipponbare, Zhonghua15, Sewon392 and JX17 were sequenced.

Table S3Sequence verification on the g237t site in domesticated indica cultivars.

Accession / Origin / g237t / Phenotype
DV 85 / Bangladesh / T / non-shattering
Aus 317 / Bangladesh / T / non-shattering
Aus 337 / Bangladesh / T / non-shattering
Aus 529 / Bangladesh / T / non-shattering
Beri / Bangladesh / T / non-shattering
Achar Bhog / Bangladesh / T / non-shattering
Bhat Mukhu / Bangladesh / T / non-shattering
Chakula / Bangladesh / T / non-shattering
Kangpur / Bangladesh / T / non-shattering
Jabahulo / Bangladesh / T / non-shattering
Kele / Bangladesh / T / non-shattering
Teqing* / China / T / non-shattering
Guangluai 4* / China / T / non-shattering
Guichao 2* / China / T / non-shattering
9311* / China / T / non-shattering
Minghui63 / China / T / non-shattering
Hezuihongnuo / China / T / non-shattering
Lucaihao / China / T / non-shattering
Heikeshu / China / T / non-shattering
Zhaiyeqing / China / T / non-shattering
Shuyazhan / China / T / non-shattering
Bayuehong / China / T / non-shattering
Hongmi / China / T / non-shattering
Huangke Xianzhan / China / T / non-shattering
Baijiaomagu / China / T / non-shattering
Menjiayang / China / T / non-shattering
Wanhuangdao / China / T / non-shattering
Yinggunian / China / T / non-shattering
Shangannian / China / T / non-shattering
Nanjing 11 / China / T / non-shattering
Zhongnong-4 / China / T / non-shattering
Xiangu / China / T / non-shattering
Zhongmaxian / China / T / non-shattering
Mengliangu / China / T / non-shattering
Dawanggu / China / T / non-shattering
Qihuangdao / China / T / non-shattering
Colombo / India / T / non-shattering
Barkat / India / T / non-shattering
Jamuna / India / T / non-shattering
Padi Kea / Indonesia / T / non-shattering
Padi Kesamint / Indonesia / T / non-shattering
Padi Adavt Dva Apt / Indonesia / T / non-shattering
GH 319 / Indonesia / T / non-shattering
Cikapunduag / Indonesia / T / non-shattering
Camor / Indonesia / T / non-shattering
Beok Montor / Indonesia / T / non-shattering
Gendjah Banten / Indonesia / T / non-shattering
Ase Bolon Kamandi / Indonesia / T / non-shattering
Bali Ketumbaran / Indonesia / T / non-shattering
Balejan / Indonesia / T / non-shattering
Kathemax / Indonesia / T / non-shattering
Bandar Tjilenma / Indonesia / T / non-shattering
Milyang 46 / Korea / T / non-shattering
Milyang 80 / Korea / T / non-shattering
RiRi 356 / Korea / T / non-shattering
RiRi 357 / Korea / T / non-shattering
Mat Camda(Zao) / Malaysia / T / non-shattering
Mab Suri / Malaysia / T / non-shattering
Muda / Malaysia / T / non-shattering
Sein Ta lay / Myanmar / T / non-shattering
Mollika(sel) / Nepal / T / non-shattering
KK15-37-C / Nepal / T / non-shattering
NR10045-20-3-2 / Nepal / T / non-shattering
O-Goo-Thou / Nepal / T / non-shattering
Liri Palmen / Pakistan / T / non-shattering
Kashmin Basmali / Pakistan / T / non-shattering
Basmati Dhan / Pakistan / T / non-shattering
Basmati Kamon / Pakistan / T / non-shattering
Basmati Hunar / Pakistan / T / non-shattering
Kalu Murunga / Pakistan / T / non-shattering
Lateefy / Pakistan / T / non-shattering
IR24 / Philippines / T / non-shattering
DoDo / Philippines / T / non-shattering
Hr33 / Philippines / T / non-shattering
R2389 / Philippines / T / non-shattering
Aclt / Philippines / T / non-shattering
Lchsannde / Philippines / T / non-shattering
Rikn-2 / Philippines / T / non-shattering
Agbede / Philippines / T / non-shattering
Hoshikalmi / Philippines / T / non-shattering
Lampung Pucih / Philippines / T / non-shattering
Tcanuto / Philippines / T / non-shattering
Barah / Philippines / T / non-shattering
Horomamawee / Sri Lanka / T / non-shattering
Polayal / Sri Lanka / T / non-shattering
RD23 / Thailand / T / non-shattering
Geluo 4 / Thailand / T / non-shattering
Naw Sadui / Thailand / T / non-shattering
XX-4 / Thailand / T / non-shattering
DM36 / Vietnam / T / non-shattering
Chiem Bua / Vietnam / T / non-shattering
Re Them Ha Dong / Vietnam / T / non-shattering
Sai Duong Thamhheai / Vietnam / T / non-shattering
Tep1 / Vietnam / T / non-shattering
Tep2 / Vietnam / T / non-shattering
DR32 / Vietnam / T / non-shattering

The p5gt and p3gt primers were used to amplify and sequence DNA fragments containing the g237t site from these rice cultivars. *, The full length sha1 cDNA from Teqing, 9311, Guangluai4 and Guichao2 were sequenced.