Table S1. Proteins Identified by MS/MS Analysis

Table S1. Proteins identified by MS/MS analysis.

High Mg2+, low salt conditions / Low Mg2+, high salt conditions
Protein Name / Accession Number / Total peptides / Unique peptides / Total peptides / Unique peptides
30S ribosomal protein S1 / P02349 / 46 / 5 / 23 / 8
30S ribosomal protein S2 / P02351 / 57 / 5 / 13 / 5
30S ribosomal protein S3 / P02352 / 34 / 4 / 27 / 5
30S ribosomal protein S4 / P02354 / 1 / 1 / 24 / 7
30S ribosomal protein S5 / P02356 / 14 / 5 / 16 / 3
30S ribosomal protein S6 / P02358 / 3 / 2 / 20 / 3
30S ribosomal protein S7 / P02359 / 18 / 5 / 21 / 4
30S ribosomal protein S8 / P02361 / 2 / 1 / 7 / 4
30S ribosomal protein S9 / P02363 / 5 / 4 / 8 / 4
30S ribosomal protein S10 / P02364 / 25 / 1 / 3 / 1
30S ribosomal protein S11 / P02366 / 8 / 3 / 6 / 2
30S ribosomal protein S13 / P02369 / 214 / 1 / 27 / 6
30S ribosomal protein S14 / P02370 / 10 / 4 / 7 / 2
30S ribosomal protein S15 / P02371 / 2 / 1 / 3 / 2
30S ribosomal protein S16 / P02372 / 11 / 1
30S ribosomal protein S17 / P02373 / 6 / 3 / 2 / 1
30S ribosomal protein S18 / P02374 / 1 / 1 / 16 / 2
30S ribosomal protein S19 / P02375 / 20 / 4 / 3 / 2
30S ribosomal protein S20 / P02378 / 19 / 4
50S ribosomal protein L5 / P62401 / 92 / 15 / 45 / 12
50S ribosomal protein L6 / P02390 / 144 / 5 / 31 / 8
50S ribosomal protein L7/L12 / P02392 / 19 / 4
50S ribosomal protein L9 / P02418 / 26 / 5 / 30 / 9
50S ribosomal protein L10 / P02408 / 5 / 2 / 23 / 5
50S ribosomal protein L11 / P02409 / 39 / 3 / 40 / 4
50S ribosomal protein L13 / P02410 / 34 / 6 / 14 / 4
50S ribosomal protein L14 / P02411 / 19 / 7 / 26 / 4
50S ribosomal protein L15 / P02413 / 56 / 6 / 56 / 9
50S ribosomal protein L16 / P02414 / 7 / 5 / 110 / 4
50S ribosomal protein L17 / P02416 / 18 / 4 / 17 / 3
50S ribosomal protein L18 / P02419 / 26 / 3 / 10 / 5
50S ribosomal protein L19 / P02420 / 139 / 6 / 21 / 4
50S ribosomal protein L20 / P02421 / 21 / 7 / 19 / 5
50S ribosomal protein L21 / P02422 / 83 / 4 / 22 / 4
50S ribosomal protein L23 / P02424 / 8 / 2 / 9 / 3
50S ribosomal protein L24 / Q8FD03 / 82 / 3 / 6 / 3
50S ribosomal protein L25 / P68919 / 161 / 5 / 38 / 4
50S ribosomal protein L27 / P02427 / 356 / 4 / 19 / 4
50S ribosomal protein L28 / P02428 / 115 / 4 / 19 / 1
50S ribosomal protein L29 / P02429 / 89 / 2 / 17 / 2
50S ribosomal protein L30 / P02430 / 48 / 3 / 44 / 1
50S ribosomal protein L31 / P02432 / 196 / 2
50S ribosomal protein L32 / P02435 / 224 / 2 / 4 / 2
50S ribosomal protein L33 / P02436 / 110 / 4 / 7 / 1
50S ribosomal protein L34 / P02437 / 14 / 3 / 4 / 1
AccC, Biotin carboxylase / P24182 / 3 / 1
AceE, Pyruvate dehydrogenase, E1 / P06958 / 260 / 25 / 71 / 19
AceF, Dihydrolipoyllysine-residue acetyltransferase, E2 / P06959 / 582 / 15 / 83 / 19
AhpF, Alkyl hydroperoxide reductase subunit F / P35340 / 2 / 1
AtpD, ATP synthase beta chain / P00824 / 1 / 1 / 3 / 2
CgtA, GTP-binding protein / P42641 / 16 / 2 / 2 / 1
CsdA, Cold-shock DEAD-box protein A / Q8XA87 / 55 / 8 / 43 / 4
Eno, Enolase / P08324 / 11 / 2 / 2 / 1
FabI, Enoyl-[acyl-carrier-protein] reductase / P29132 / 2 / 1
FolX, D-erythro-7,8-dihydroneopterin triphosphate epimerase / P80449 / 2 / 1
GlpD, Glycerol-3-phosphate dehydrogenase / P13035 / 5 / 1
GuaA, GMP synthas / P04079 / 5 / 1
HslR, Hsp15, Heat shock protein 15 / P45802 / 7 / 2
CycB, transcriptional regulator / P06613 / 2 / 1
IclR, Acetate operon repressor / P16528 / 2 / 1
IncC, ParA-like / P07673 / 8 / 2
InfC, Tranlation Initiation factor 3 / P02999 / 4 / 1
LacZ, Beta-galactosidase / P00722 / 6 / 2
LipA, Lipoyl synthase / P60717 / 2 / 2
LpdA, Dihydrolipoyl dehydrogenase, E3 / P00391 / 141 / 12 / 32 / 8
MdoH, membrane glycosyltransferase / Q8FIS3 / 4 / 1
NagC, N-acetylglucosamine repressor / P15301 / 5 / 1
OmpA, outer membrane protein A / P02934 / 41 / 5
OmpC, outer membrane protein C / Q8XE41 / 15 / 3
OppD, Oligopeptide transport ATP-binding protein / P76027 / 2 / 1
Pal, Peptidoglycan-associated lipoprotein / P07176 / 5 / 4
PheT, Phenylalanyl-tRNA synthetase beta chain / Q8XE32 / 2 / 1
Pnp, Polyribonucleotide nucleotidyltransferase / P05055 / 11 / 2 / 2 / 1
ProQ, ProP effector / P45577 / 4 / 1 / 1 / 1
Prs, Ribose-phosphate pyrophosphokinase / P08330 / 12 / 2 / 2 / 1
PssA, Phosphatidylserine synthase / P23830 / 2 / 2
Pta, Phosphate acetyltransferase / P39184 / 2 / 1
RhlB, ATP-dependent RNA helicase / P24229 / 10 / 3
RhlE, ATP-dependent RNA helicase / P25888 / 2 / 2
RluB, Pseudouridine synthase B / Q8X4Q8 / 6 / 3 / 4 / 2
RluC, Pseudouridine synthase C / Q8X8J3 / 25 / 5
Rne, RnaseE, Ribonuclease E / P21513 / 26 / 5 / 16 / 5
RpoA, RNAP alpha subunit / P0A7Z4 / 28 / 3 / 11 / 4
RpoB, RNAP beta subunit / P0A8V3 / 64 / 6 / 19 / 7
RpoC, RNAP beta' subunit / Q8FB83 / 35 / 8 / 21 / 9
RrmJ, Ribosomal RNA large subunit methyltransferase J / P28692 / 2 / 1
SrmB, ATP-dependent RNA helicase / P21507 / 25 / 4
SucA, 2-oxoglutarate dehydrogenase, E1 / P07015 / 18 / 7 / 5 / 2
SucB, Dihydrolipoyllysine-residue succinyltransferase, E2 / P07016 / 3 / 1 / 15 / 2
SucD, Succinyl-CoA synthetase alpha chain / P07459 / 4 / 1
Tig, Trigger factor (TF) / P22257 / 8 / 2 / 4 / 2
TkrA, 2-ketogluconate reductase / P37666 / 7 / 1
TnaA, Tryptophanase / Q8XB34 / 6 / 1
TopA, DNA topoisomerase I Omega-protein / P06612 / 2 / 1
TufB, Elongation factor Tu / P02990 / 16 / 2 / 10 / 3
YbaN, Hypothetical protein / P45808 / 5 / 1
YbeB, Hypothetical protein / P05848 / 4 / 1
YbjL, Putative transport protein / P60871 / 2 / 1
YdbL, Hypothetical protein / P76076 / 3 / 1
YeiP, Elongation factor P-like protein / P33028 / 7 / 1
YejB, Hypothetical ABC transporter permease / P77279 / 2 / 2
YggN, Hypothetical protein / P46143 / 4 / 1
YghO, Hypothetical protein / Q46840 / 4 / 1
YhbY, Hypothetical protein / P42550 / 42 / 2 / 3 / 2
YhcD Hypothetical outer membrane usher protein / P45420 / 1 / 1 / 3 / 1
YibL, Hypothetical protein / P36564 / 15 / 1
YjaH, Hypothetical protein / P32681 / 2 / 1
YjfJ, Hypothetical protein / P39292 / 2 / 1
YjgA, Hypothetical protein / P26650 / 42 / 4
YneE, Hypothetical protein / Q8XAZ3 / 4 / 1

Table S2. Proteins identified by a single parent ion.

Protein Name / Accession Number
High Mg2+, low salt conditions
30S ribosomal protein S12 / P02367
Cdh, CDP-diacylglycerol pyrophosphatase / P06282
GapA, Glyceraldehyde-3-phosphate dehydrogenase A / P06977
GltA, Citrate synthase / P00891
InfB, IF-2, Translation initiation factor / P59587
LysS, Lysyl-tRNA synthetase / P13030
NrdA, Ribonucleoside-diphosphate reductase 1 alpha subunit / P00452
NusG, Transcription antitermination protein / P16921
Ppa, Inorganic pyrophosphatase / P17288
PtsP, Phosphoenolpyruvate-protein phosphotransferase / P37177
PurA, Adenylosuccinate synthetase / P12283
Rnr, RnaseR, Ribonuclease / P21499
SdhB, Succinate dehydrogenase iron-sulfur protein / P07014
Tgt, Queuine tRNA-ribosyltransferase / P19675
YdfG, NADP-dependent L-serine/L-allo-threonine dehydrogenase / Q8X505
YeaP, Hypothetical protein / P76245
YfiF, Hypothetical tRNA/rRNA methyltransferase / P33635
Zwf, Glucose-6-phosphate 1-dehydrogenase / P22992
Low Mg2+, high salt conditions
AceA, Isocitrate lyase / P05313
Bla, Beta-lactamase / P14558
CpxA, histidine kinase / P08336
EutT, Ethanolamine utilization cobalamin adenosyltransferase / P65643
FabZ, (3R)-hydroxymyristoyl-[acyl carrier protein / P21774
FumA, Fumarate hydratase class I / P00923
GroEL, 60 kDa chaperonin / P06139
IciA, Chromosome initiation inhibitor / P24194
MobA, Molybdopterin-guanine dinucleotide biosynthesis protein A / P32173
OmpX, Outer membrane protein X / P36546
PtsA, Phosphoenolpyruvate-protein phosphotransferase / P32670
RecA, Recombinase A / P03017
SecD, Protein-export membrane protein / P19673
TkrA, 2-ketogluconate reductase / P58220
TyrA, T-protein / P07023
YagH, Putative beta-xylosidase / P77713
YajO, Hypothetical protein / P77735
YlbA, Hypothetical protein / P75713
YraM, Hypothetical protein / P45464