Table S1. Percentage of cells with O2- in roots treated with specific inhibitors and infected by different species of H. annosum sensu lato (average value ± SE). Values with the same uppercase letter within each horizontal row or the same lowercase letter within each vertical column do not differ statistically from one another (Tukey’s test, P 0.05).

4h / P for the row / 48h / P for the row
control / H. annosum s.s. / H. parviporum / H. abietinum / control / H. annosum s.s. / H. parviporum / H. abietinum
control / 0
b / 3.18 ± 0.99 / 6.44 2.67 / 0
b / 0.39 ± 0.34 / 1.78 ± 1.34
KCN / 0.48 ± 0.17
b, B / 14.44 ± 2.94
a, A / 1.14 ± 0.26
B / 6.35 ± 2.20
B / P < 0.001 / 0
b, AB / 4.62 ± 2.46
b, A / 0.37 ± 0.25
B / 0.95 ± 0.62
AB / P < 0.020
NaN3 / 2.10 ± 0.47
ab, B / 21.85 ± 4.85
a, A / 0.83 ± 0.27
B / 6.49 ± 2.23
B / P < 0.001 / 0
b, AB / 2.35 ± 1.44
b, A / 0.10 ± 0.15
B / 0.13 ± 0.15
B / P < 0.006
DPI / 0.33 ± 0.19
b / 7.28 ± 3.86
b / 1.25 ± 0.59 / 5.17 ± 1.78 / P < 0.051 / 0.17 ± 0.16
b / 0.25 ± 0.43
b / 0.63 ± 0.61 / 0.13 ± 0.19 / P < 0.450
ATZ / 7.33 ± 1.58
a, A / 5.76 ± 2.32
b, A / 1.22 ± 0.61
B / 5.56 ± 1.86
A / P < 0.042 / 1.67 ± 0.62
a, B / 14.57 ± 4.88
a, A / 0.78 ± 0.51
B / 0.17 ± 0.28
B / P < 0.001
acid / 2.00 ± 0.61
ab, AB / 19.00 ± 6.54
a, A / 1.24 ± 0.52
B / 5.42 ± 2.86
B / P < 0.001 / 0
B / 10.74 ± 3.97
b, A / 0.83 ± 0.51
B / 1.68 ± 1.76
B / P < 0.001
DFO / 2.86 ± 1.67
b, B / 0.67 ± 0.28
b, B / 2.92 ± 1.24
B / 10.13 ± 2.33
A / P < 0.001 / 0.29 ± 0.17
ab / 1.24 ± 0.99
b / 1.02 ± 0.74 / 0.19 ± 0.17 / P < 0.400
P for the column / P < 0.006 / P < 0.001 / P < 0.069 / P < 0.081 / P < 0.007 / P < 0.001 / P < 0.355 / P < 0.178

Table S2. Percentage of cells with Fe3+ in roots treated with specific inhibitors and infected by different species of H. annosum sensu lato (average value ± SE). Values with the same uppercase letter within each horizontal row or the same lowercase letter within each vertical column do not differ statistically from one another (Tukey’s test, P 0.05).

4h / P for the row / 48h / P for the row
control / H. annosum s.s. / H. parviporum / H. abietinum / control / H. annosum s.s. / H. parviporum / H. abietinum
control / 11.56 ± 3.80
abc / 7.83 ± 3.00
a / 8.91 ± 3.39
a / 1.94 ± 1.72
b / 3.62 ± 3.01 / 13.95 ± 3.91
a
KCN / 0
b / 3.86 ± 1.58
c / 0.45 ± 0.53
b / 2.57 ± 1.42
b / P < 0.001 / 1.75± 1.62
b / 12.99 ± 2.95
a / 0.90 ± 0.88 / 3.81 ± 1.88
b / P < 0.020
NaN3 / 0.78 ± 0.72
ab / 12.49 ± 1.81
ab / 0.32 ± 0.55
b / 3.51 ± 1.61
b / P < 0.001 / 31.19± 1.19
a / 15.32 ± 3.85
a / 0.87 ± 0.86 / 8.10 ± 2.38
ab / P < 0.006
DPI / 0
b / 16.61 ± 3.93
a / 2.36 ± 1.48
b / 2.97 ± 2.18
b / P < 0.051 / 2.99± 1.95
b / 15.30 ± 4.67
a / 0.76 ± 0.74 / 6.54 ± 2.71
b / P < 0.450
ATZ / 4.30 ± 1.64
a / 11.17 ± 5.44
abc / 4.39 ± 2.37
ab / 2.15 ± 1.41
b / P < 0.042 / 2.96± 1.49
b / 10.39 ± 2.91
a / 0.71 ± 1.23 / 2.55 ± 1.53
b / P < 0.001
acid / 2.01 ± 0.95
ab / 7.93 ± 2.62
bc / 4.67 ± 1.88
ab / 2.02 ± 1.49
b / P < 0.001 / 0
b / 18.88 ± 5.47
a / 0 / 5.16 ± 2.56
b / P < 0.001
DFO / 0.67 ± 0.67
b / 5.10 ± 1.55
bc / 3.05 ± 1.44
b / 2.51 ± 1.49
b / P < 0.001 / 0.67± 0.67
b / 2.58 ± 1.23
b / 1.72 ± 1.50 / 2.40 ± 1.51
b / P < 0.400
P for the column / P < 0.009 / P < 0.001 / P < 0.001 / P < 0.001 / P < 0.001 / P < 0.001 / P < 0.058 / P < 0.001

Table S3. Percentage of cells with Fe3+ and H2O2 co-localizationin roots treated with specific inhibitors and infected by different species of H. annosum sensu lato (average value ± SE). Values with the same uppercase letter within each horizontal row or the same lowercase letter within each vertical column do not differ statistically from one another (Tukey’s test, P 0.05).

4h / P for the row / 48h / P for the row
control / H. annosum s.s. / H. parviporum / H. abietinum / control / H. annosum s.s. / H. parviporum / H. abietinum
control / 6.13 ± 2.92
a / 0.55 ± 0.75 / 2.16 ± 1.31
ab / 8.42 ± 2.59
a / 1.76 ± 1.12
ab / 7.25 ± 2.22
a
KCN / 0 / 0.39 ± 0.50
b / 0.41 ± 0.69 / 0.78 ± 0.83
bc / P < 0.718 / 0
B / 3.52 ± 1.50
b, A / 0.83 ± 0.83
ab, B / 1.85 ± 1.20
b, AB / P < 0.019
NaN3 / 0 / 0
b / 0 / 0
c / - / 0 / 0
b / 0.47 ± 0.55
ab / 1.12 ± 1.02
b / P < 0.183
DPI / 0 / 0
b / 0.70 ± 0.65 / 0.19 ± 0.35
c / P < 0.170 / 0
B / 2.72 ± 1.74
b, A / 2.45 ± 1.70
a, AB / 0
b, B / P0.022
ATZ / 0 / 0.94 ± 0.98
b / 0.61 ± 0.76 / 0.53 ± 0.69
bc / P < 0.743 / 0
B / 0
b, B / 0
b, B / 1.04 ± 0.94
b, A / P < 0.045
acid / 0
B / 0
b, B / 0.67 ± 0.64
A / 0
c, B / P < 0.044 / 0 / 0.89 ± 1.03
b / 0
b / 0
b / P < 0.126
DFO / 0
B / 0.45 ± 0.53
b / 0.54 ± 0.65
B / 3.00 ± 1.28
a, A / P < 0.001 / 0 / 1.50 ± 1.43
b / 0
b / 1.76 ± 1.38
b / P < 0.153
P for the column / - / P < 0.001 / P < 0.870 / P < 0.001 / - / P < 0.001 / P < 0.002 / P < 0.001

Table S4.Percentage of cells with Fe2+ in roots treated with specific inhibitors and infected by different species of H. annosum sensu lato (average value ± SE). Values with the same uppercase letter within each horizontal row or the same lowercase letter within each vertical column do not differ statistically from one another (Tukey’s test, P 0.05).

4h / P for the row / 48h / P for the row
control / H. annosum s.s. / H. parviporum / H. abietinum / control / H. annosum s.s. / H. parviporum / H. abietinum
control / 3.27 ± 2.51 / 5.38 ± 2.38
a / 1.30 ± 0.92 / 10.99 ± 2.39
a / 0
b / 0.32 ± 0.55
b
KCN / 0.67 ± 0.67
ab / 1.37 ± 0.99 / 2.04 ± 1.21
bc / 1.12 ± 0.96 / P < 0.592 / 2.78 ± 1.36
ab, AB / 0.67 ± 0.85
b, B / 4.01 ± 2.23
a, A / 0
b, B / P < 0.004
NaN3 / 0
b / 1.88 ± 1.68 / 0.50 ± 0.91
c / 0.67 ± 0.64 / P < 0.334 / 0.67 ± 0.67
bc, AB / 3.72 ± 2.30
b, A / 0.49 ± 0.63
b, B / 0
b, B / P < 0.005
DPI / 0
b / 2.38 ± 1.63 / 1.08 ± 0.88
bc / 1.60 ± 1.16 / P < 0.324 / 2.96 ± 1.08
a, AB / 8.41 ± 3.19
a, A / 0.57 ± 0.67
b, B / 0
b, B / P < 0.001
ATZ / 2.56 ± 1.23
a, AB / 2.64 ± 1.85
AB / 3.63 ± 1.28
ab, A / 0.54 ± 0.65
B / P < 0.048 / 0
c / 0.82 ± 1.00
b / 1.02 ± 0.89
b / 0
b / P < 0.279
acid / 0
b, B / 4.72 ± 1.68
A / 0.76 ± 0.74
bc, B / 0.22 ± 0.39
B / P < 0.001 / 0
c, B / 2.49 ± 1.65
b, A / 1.87 ± 1.17
ab, AB / 0
b, B / P < 0.028
DFO / 0
b / 0.54 ± 0.65 / 0
c / 0 / P < 0.197 / 0
c, B / 0
b, B / 0
b, B / 1.93 ± 1.04
a, A / P < 0.001
P for the column / P < 0.021 / P < 0.073 / P < 0.001 / P < 0.115 / P < 0.012 / P < 0.001 / P < 0.001 / P < 0.001

Table S5.Percentage of cells with Fe2+ and H2O2 co-localization in roots treated with specific inhibitors and infected by different species of H. annosum sensu lato (average value ± SE). Values with the same uppercase letter within each horizontal row or the same lowercase letter within each vertical column do not differ statistically from one another (Tukey’s test, P 0.05).

4h / P for the row / 48h / P for the row
control / H. annosum s.s. / H. parviporum / H. abietinum / control / H. annosum s.s. / H. parviporum / H. abietinum
control / 33.68 ± 7.14
a / 0.49 ± 0.36
ab / 0.48 ± 0.25 / 17.52 ± 4.49
a / 0.06 ± 0.08
b / 0.70 ± 0.39
ab
KCN / 1.24 ± 1.03
AB / 6.88 ± 4.40
b, A / 0.25 ± 0.25
b, B / 0.03 ± 0.05
B / P < 0.002 / 0
B / 0.77 ± 0.69
bc, A / 0.44 ± 0.33
b, AB / 0.06 ± 0.08
b, B / P < 0.041
NaN3 / 0.48 ± 0.32
AB / 1.57 ± 1.45
bc, A / 0.10 ± 0.09
b, B / 0.06 ± 0.08
B / P < 0.019 / 0
B / 0.76 ± 0.52
bc, A / 0.10 ± 0.09
b, B / 0.09 ± 0.12
b, B / P < 0.003
DPI / 3.14 ± 1.50
A / 0.97 ± 0.83
bc, AB / 0.13 ± 0.17
b, B / 0.24 ± 0.18
B / P < 0.002 / 0
B / 0.22 ± 0.15
c, A / 0
b, B / 0.16 ± 0.16
ab, AB / P < 0.026
ATZ / 0
B / 6.89 ± 3.95
b, A / 0
b, B / 0.19 ± 0.23
B / P < 0.001 / 0.10 ± 0.10
B / 4.03 ± 1.89
b, A / 0.06 ± 0.11
b, B / 0.79 ± 0.47
ab, B / P < 0.001
acid / 0.86 ± 0.56
A / 0
c, B / 0.16 ± 0.19
b, AB / 0.16 ± 0.14
AB / P < 0.042 / 0.67 ± 0.48 / 0.14 ± 0.16
c / 0
b / 0.13 ± 0.13
ab / P < 0.065
DFO / 0.76 ± 0.47 / 0.38 ± 0.32
bc / 1.30 ± 0.73
a / 0.57 ± 0.72 / P < 0.153 / 0.67 ± 0.38 / 1.59 ± 1.05
bc / 2.25 ± 1.11
a / 1.05 ± 0.70
a / P < 0.454
P for the column / P < 0.191 / P < 0.001 / P < 0.001 / P < 0.163 / P < 0.112 / P < 0.001 / P < 0.001 / P < 0.002