Table S1. General collection localities and number of rotan Perccottus glenii sampled from five regions of Russia. All fish from the Ilev site were genotyped at nine microsatellite DNA loci. Two fish from each collection were all pooled to generate a genomic DNA shotgun library for sequencing on the Ion Torrent Personal Genome Machine.
General collection location(water body) / Drainage
basin / Province / Coordinates / Sample
Size (N)
Latitude / Longitude
Tarakanovo Pond / Volga / Moscow / 55º 44’ / 36º 34’ / 31
Ilev / Volga / Nizhniy Novgorod / 54º 57’ / 43º 02’ / 35
Lake Bolshoe Klukvennoe / Volga / Samara / 53º 14’ / 49º 51’ / 35
Lake Maloe Vetino / Volga / Ryazan / 54º 42’ / 40º 58’ / 29
Lake Bolshoe Shchuryachie / Don / Voronezh / 51º 13’ / 41º 42’ / 29
Table S2. Summarization of the quality-based variant detection resulting from Ion Torrent Personal Genome Machine reads generated in 10 fish sampled from five Russian populations and mapped to the complete mtDNA sequence of rotan (Perccottus glenii) (accession number NC_020350) (Xue et al. 2013). The table includes reference position of the variant (relative to NC_020350), type of variant (single (SNV) or multiple (MNV) nucleotide), reference allele, variant allele, the number of occurrences of the variant allele, the sequence read coverage at that position, frequency of the variant allele, average quality (PHRED) score at the reference position, and the annotated region of the mtDNA sequence variant. Annotation abbreviations are as follows: NADH dehydrogenase (NAD) and cytochrome oxidase (COX). The putative phylogeographic utility of each site based on whether the variant is monomorphic within the Eurasian samples yet diagnostic relative to the Asian sequence and thus potentially of use in determining the source of the introduction or if the site is polymorphic and of utility in describing population genetic structure among the Russian collections.
Reference Position / Type / Reference allele / Variant allele(s) / Count / Coverage / Frequency / Average quality / Annotation / Phylogeographic utility260 / SNV / G / A / 72 / 72 / 100.0 / 31 / 12S rRNA / Diagnostic
544 / SNV / C / T / 36 / 51 / 70.6 / 30 / 12S rRNA / Polymorphic
714 / SNV / T / C / 68 / 68 / 100.0 / 31 / 12S rRNA / Diagnostic
795 / SNV / C / T / 72 / 77 / 93.5 / 31 / 12S rRNA / Polymorphic
858 / SNV / A / G / 63 / 63 / 100.0 / 31 / 12S rRNA / Diagnostic
949 / SNV / T / C / 32 / 37 / 86.5 / 31 / 12S rRNA / Polymorphic
1323 / SNV / C / T / 59 / 61 / 96.7 / 31 / 12S rRNA / Diagnostic
1524 / SNV / G / A / 45 / 45 / 100.0 / 31 / 12S rRNA / Diagnostic
1676 / SNV / C / T / 76 / 76 / 100.0 / 31 / 12S rRNA / Diagnostic
1723 / SNV / C / T / 70 / 71 / 98.6 / 32 / 12S rRNA / Diagnostic
2260 / SNV / C / T / 67 / 67 / 100.0 / 32 / 12S rRNA / Diagnostic
2274 / SNV / T / C / 54 / 57 / 94.7 / 31 / 12S rRNA / Polymorphic
2369 / SNV / T / C / 46 / 46 / 100.0 / 30 / 12S rRNA / Diagnostic
2678 / SNV / T / C / 38 / 40 / 95.0 / 31 / 12S rRNA / Diagnostic
2842 / SNV / T / C / 68 / 68 / 100.0 / 31 / NAD1 / Diagnostic
3194 / SNV / A / G / 42 / 49 / 85.7 / 31 / NAD1 / Polymorphic
3392 / SNV / A / G / 40 / 47 / 85.1 / 31 / NAD1 / Polymorphic
3491 / SNV / T / C / 37 / 43 / 86.0 / 31 / NAD1 / Polymorphic
3509 / SNV / C / T / 30 / 33 / 90.9 / 31 / NAD1 / Polymorphic
3629 / MNV / TT / CC / 30 / 35 / 85.7 / 31 / NAD1 / Diagnostic
3785 / SNV / A / G / 26 / 26 / 100.0 / 31 / NAD1 / Diagnostic
4314 / SNV / C / T / 28 / 28 / 100.0 / 31 / NAD2 / Diagnostic
4347 / SNV / T / C / 17 / 25 / 68.0 / 32 / NAD2 / Polymorphic
4374 / SNV / A / G / 49 / 49 / 100.0 / 32 / NAD2 / Diagnostic
4467 / SNV / T / C / 54 / 57 / 94.7 / 30 / NAD2 / Polmorphic
4491 / SNV / T / C / 49 / 49 / 100.0 / 32 / NAD2 / Diagnostic
4495 / SNV / G / A / 54 / 54 / 100.0 / 31 / NAD2 / Diagnostic
4626 / SNV / A / G / 32 / 34 / 94.1 / 29 / NAD2 / Polymorphic
4677 / SNV / A / G / 41 / 49 / 83.7 / 31 / NAD2 / Polymorphic
4776 / SNV / A / G / 8 / 32 / 25.0 / 17 / NAD2 / Polymorphic
4806 / SNV / A / G / 41 / 49 / 83.7 / 31 / NAD2 / Polymorphic
4828 / SNV / G / A / 48 / 48 / 100.0 / 31 / NAD2 / Diagnostic
4881 / SNV / T / C / 45 / 45 / 100.0 / 31 / NAD2 / Diagnostic
5016 / SNV / C / A / 33 / 35 / 94.3 / 32 / NAD2 / Polymorphic
5031 / SNV / C / A / 34 / 35 / 97.1 / 31 / NAD2 / Polymorphic
5239 / SNV / C / A / 32 / 41 / 78.0 / 31 / tRNA-Asn / Polymorphic
5279 / SNV / G / A / 62 / 62 / 100.0 / 31 / tRNA-Asn / Diagnostic
5471 / SNV / G / A / 39 / 39 / 100.0 / 32 / COX1 / Diagnostic
5574 / SNV / T / C / 64 / 64 / 100.0 / 31 / COX1 / Diagnostic
5630 / SNV / A / G / 41 / 41 / 100.0 / 29 / COX1 / Diagnostic
5750 / SNV / T / C / 52 / 62 / 83.9 / 31 / COX1 / Polymorphic
5837 / SNV / T / A / 55 / 59 / 93.2 / 32 / COX1 / Polymorphic
5933 / SNV / G / A / 56 / 56 / 100.0 / 32 / COX1 / Diagnostic
5936 / SNV / T / C / 44 / 44 / 100.0 / 30 / COX1 / Diagnostic
6152 / SNV / T / C / 65 / 65 / 100.0 / 32 / COX1 / Diagnostic
6266 / SNV / G / A / 67 / 67 / 100.0 / 31 / COX1 / Diagnostic
6320 / SNV / C / T / 85 / 85 / 100.0 / 31 / COX1 / Diagnostic
6674 / SNV / C / T / 83 / 83 / 100.0 / 32 / COX1 / Diagnostic
6698 / SNV / G / A / 57 / 57 / 100.0 / 32 / COX1 / Diagnostic
6755 / MNV / T / G/A / 19/7 / 26 / 73.1/26.9 / 31 / COX1 / Polymorphic
6767 / SNV / G / A / 28 / 28 / 100.0 / 31 / COX1 / Diagnostic
7037 / SNV / G / A / 46 / 46 / 100.0 / 31 / tRNA-Ser / Diagnostic
7354 / SNV / G / A / 57 / 57 / 100.0 / 29 / COX2 / Diagnostic
7435 / SNV / C / T / 11 / 32 / 34.4 / 32 / COX2 / Polymorphic
7471 / SNV / G / A / 68 / 68 / 100.0 / 31 / COX2 / Diagnostic
7477 / SNV / G / A / 61 / 61 / 100.0 / 30 / COX2 / Diagnostic
7741 / SNV / G / A / 64 / 64 / 100.0 / 32 / COX2 / Diagnostic
7825 / SNV / G / A / 56 / 56 / 100.0 / 33 / COX2 / Diagnostic
8272 / SNV / C / T / 41 / 41 / 100.0 / 31 / ATPase6 / Diagnostic
8284 / SNV / C / T / 34 / 34 / 100.0 / 31 / ATPase6 / Diagnostic
8347 / SNV / G / A / 51 / 51 / 100.0 / 32 / ATPase6 / Diagnostic
8392 / SNV / G / A / 48 / 48 / 100.0 / 31 / ATPase6 / Diagnostic
8927 / SNV / A / G / 68 / 70 / 97.1 / 32 / COX3 / Polymorphic
8983 / SNV / T / C / 55 / 55 / 100.0 / 30 / COX3 / Diagnostic
8999 / SNV / T / C / 45 / 50 / 90.0 / 31 / COX3 / Polymorphic
9083 / SNV / A / G / 30 / 30 / 100.0 / 28 / COX3 / Diagnostic
9116 / SNV / C / T / 40 / 40 / 100.0 / 32 / COX3 / Diagnostic
9152 / SNV / G / A / 39 / 39 / 100.0 / 32 / COX3 / Diagnostic
9185 / SNV / G / A / 49 / 63 / 77.8 / 31 / COX3 / Polymorphic
9482 / SNV / G / A / 42 / 42 / 100.0 / 30 / COX3 / Diagnostic
9488 / SNV / T / C / 31 / 39 / 79.5 / 31 / COX3 / Polymorphic
9707 / SNV / C / T / 61 / 61 / 100.0 / 31 / NAD3 / Diagnostic
9710 / SNV / C / A / 67 / 67 / 100.0 / 31 / NAD3 / Diagnostic
9737 / SNV / G / A / 51 / 51 / 100.0 / 30 / NAD3 / Diagnostic
9923 / SNV / G / A / 33 / 40 / 82.5 / 30 / NAD3 / Polymorphic
10086 / SNV / A / G / 51 / 51 / 100.0 / 29 / NAD4L / Diagnostic
10218 / SNV / T / C / 38 / 38 / 100.0 / 31 / NAD4L / Diagnostic
10278 / SNV / C / T / 77 / 77 / 100.0 / 31 / NAD4L / Diagnostic
10472 / SNV / C / T / 35 / 37 / 94.6 / 32 / NAD4 / Polymorphic
10527 / SNV / C / T / 38 / 38 / 100.0 / 29 / NAD4 / Diagnostic
10559 / SNV / A / G / 29 / 33 / 87.9 / 31 / NAD4 / Polymorphic
10592 / SNV / G / A / 27 / 35 / 77.1 / 32 / NAD4 / Polymorphic
10623 / SNV / T / C / 41 / 47 / 87.2 / 32 / NAD4 / Polymorphic
10856 / SNV / A / C / 33 / 33 / 100.0 / 30 / NAD4 / Diagnostic
10911 / SNV / T / C / 30 / 30 / 100.0 / 31 / NAD4 / Diagnostic
11126 / SNV / C / A / 48 / 48 / 100.0 / 33 / NAD4 / Diagnostic
11138 / MNV / CG / TA / 38 / 38 / 100.0 / 32 / NAD4 / Diagnostic
11252 / SNV / G / A / 46 / 57 / 80.7 / 31 / NAD4 / Polymorphic
11294 / SNV / A / G / 57 / 66 / 86.4 / 32 / NAD4 / Polymorphic
11361 / SNV / C / T / 46 / 46 / 100.0 / 30 / NAD4 / Diagnostic
11402 / SNV / G / A / 61 / 61 / 100.0 / 31 / NAD4 / Diagnostic
11528 / SNV / A / G / 30 / 30 / 100.0 / 31 / NAD4 / Diagnostic
11600 / SNV / T / C / 27 / 31 / 87.1 / 31 / NAD4 / Polymorphic
11636 / SNV / A / G / 25 / 30 / 83.3 / 32 / NAD4 / Polymorphic
11712 / SNV / G / A / 39 / 39 / 100.0 / 31 / tRNA-His / Diagnostic
11725 / SNV / C / T / 36 / 36 / 100.0 / 32 / tRNA-His / Diagnostic
11765 / SNV / C / T / 33 / 35 / 94.3 / 30 / tRNA-His / Polymorphic
11947 / SNV / G / A / 55 / 55 / 100.0 / 29 / NAD5 / Diagnostic
12181 / SNV / G / A / 38 / 38 / 100.0 / 30 / NAD5 / Diagnostic
12206 / SNV / G / A / 60 / 67 / 89.6 / 31 / NAD5 / Polymorphic
12232 / SNV / G / A / 12 / 59 / 20.3 / 32 / NAD5 / Polymorphic
12349 / SNV / G / A / 60 / 64 / 93.8 / 31 / NAD5 / Polymorphic
12376 / SNV / T / G / 59 / 59 / 100.0 / 32 / NAD5 / Diagnostic
12481 / SNV / G / A / 39 / 45 / 86.7 / 31 / NAD5 / Polymorphic
12550 / SNV / A / T / 95 / 95 / 100.0 / 32 / NAD5 / Diagnostic
12578 / SNV / T / C / 74 / 81 / 91.4 / 31 / NAD5 / Polymorphic
12626 / SNV / T / C / 48 / 48 / 100.0 / 30 / NAD5 / Diagnostic
12697 / SNV / G / A / 63 / 63 / 100.0 / 32 / NAD5 / Diagnostic
12778 / SNV / T / C / 48 / 48 / 100.0 / 30 / NAD5 / Diagnostic
13018 / SNV / G / A / 58 / 58 / 100.0 / 31 / NAD5 / Diagnostic
13087 / SNV / A / G / 23 / 27 / 85.2 / 31 / NAD5 / Polymorphic
13123 / SNV / T / C / 36 / 36 / 100.0 / 30 / NAD5 / Diagnostic
13240 / SNV / G / A / 41 / 41 / 100.0 / 31 / NAD5 / Diagnostic
13666 / SNV / T / C / 29 / 29 / 100.0 / 32 / NAD5 / Diagnostic
14090 / SNV / T / C / 30 / 30 / 100.0 / 33 / NAD5 / Diagnostic
14584 / SNV / C / T / 43 / 43 / 100.0 / 30 / cytochrome b / Diagnostic
14601 / SNV / C / T / 51 / 56 / 91.1 / 31 / cytochrome b / Polymorphic
14721 / SNV / T / C / 54 / 62 / 87.1 / 31 / cytochrome b / Polymorphic
14749 / SNV / C / T / 43 / 43 / 100.0 / 29 / cytochrome b / Diagnostic
14790 / SNV / C / T / 33 / 40 / 82.5 / 29 / cytochrome b / Polymorphic
14958 / SNV / A / G / 68 / 74 / 91.9 / 31 / cytochrome b / Polymorphic
14988 / SNV / T / C / 60 / 67 / 89.6 / 31 / cytochrome b / Polymorphic
15030 / SNV / T / C / 66 / 66 / 100.0 / 31 / cytochrome b / Diagnostic
15040 / SNV / C / T / 68 / 68 / 100.0 / 33 / cytochrome b / Diagnostic
15171 / SNV / C / T / 52 / 52 / 100.0 / 30 / cytochrome b / Diagnostic
15261 / SNV / G / A / 36 / 44 / 81.8 / 31 / cytochrome b / Polymorphic
15264 / SNV / A / G / 34 / 40 / 85.0 / 31 / cytochrome b / Polymorphic
15276 / SNV / C / T / 33 / 33 / 100.0 / 31 / cytochrome b / Diagnostic
15862 / SNV / G / A / 87 / 87 / 100.0 / 32 / control region / Diagnostic
16071 / SNV / T / A / 69 / 69 / 100.0 / 29 / control region / Diagnostic