Table S1. Ethylene Emission Ratio Between the Nils in This Study and LA4024

Figure 1S. Examples of different patterns of the ration between the lines and the control LA4024 of ethylene emissions across ripening. Ethylene emissions from fruits harvested at breaker (day 1) and in the following days (days 2-8) were determined. The panel on the left shows different patterns of emissions for some lines with high ethylene evolution; the panel on the right illustrates lines with low ethylene emissions. Numbers refer to the QTL designation in Table 2 and Figure 3.

Supplementary Tables

Table S1. Ethylene emission ratio between the NILs in this study and LA4024.

line / stage / ratio / SE / DF / raw p / FDR
LA3913 / 2 / 1.32 / 0.17 / 1690 / 0.01868651 / 0.04724515
6 / 1.31 / 0.17 / 1965 / 0.02728107 / 0.0652797
LA 3918 / 2 / 0.91 / 0.16 / 1690 / 0.37231297 / 0.47514227
6 / 1.08 / 0.17 / 1965 / 0.49475332 / 0.5770755
LA 3921 / 2 / 1 / 0.14 / 1690 / 0.97232638 / 0.97232638
3 / 1.03 / 0.26 / 263 / 0.79538717 / 0.83922741
4 / 1.17 / 0.17 / 361 / 0.19740703 / 0.30328918
5 / 1.29 / 0.14 / 1965 / 0.16470908 / 0.26915874
6 / 1.19 / 0.18 / 338 / 0.08070151 / 0.15578005
LA 3922 / 2 / 3.16 / 0.16 / 1690 / 3.1366E-24 / 4.20E-22
3 / 1.61 / 0.31 / 263 / 0.00296623 / 0.00993686
4 / 1.09 / 0.34 / 167 / 0.49525136 / 0.5770755
5 / 1.1 / 0.17 / 361 / 0.65179902 / 0.72182701
6 / 2.27 / 0.15 / 1965 / 5.9577E-15 / 1.14E-13
LA 3923 / 2 / 2.02 / 0.23 / 338 / 2.0742E-06 / 1.74E-05
3 / 1.37 / 0.21 / 1690 / 0.03374585 / 0.07796455
4 / 1.17 / 0.33 / 263 / 0.17656151 / 0.28505112
5 / 1.28 / 0.35 / 167 / 0.28427283 / 0.38869958
6 / 1.71 / 0.17 / 361 / 9.9368E-05 / 0.00051213
LA 3926 / 2 / 0.83 / 0.20 / 1965 / 0.15664062 / 0.25913387
6 / 1.2 / 0.21 / 338 / 0.20143833 / 0.30328918
LA 3927 / 2 / 1.58 / 0.16 / 1690 / 0.00011729 / 0.00056131
6 / 1.65 / 0.40 / 263 / 5.4876E-05 / 0.00029414
LA 3931 / 2 / 1.43 / 0.32 / 167 / 0.0015184 / 0.00581331
6 / 1.01 / 0.18 / 361 / 0.95268352 / 0.95984655
LA 3932 / 2 / 0.86 / 0.15 / 1965 / 0.20900133 / 0.3077602
6 / 1.36 / 0.20 / 338 / 0.01129111 / 0.03152103
LA 3934 / 2 / 1.25 / 0.19 / 1690 / 0.02878247 / 0.06766404
3 / 0.88 / 0.21 / 1965 / 0.3438726 / 0.44736824
4 / 1.18 / 0.17 / 1690 / 0.20128579 / 0.30328918
5 / 1.19 / 0.18 / 1965 / 0.3682996 / 0.47453987
6 / 1.82 / 0.16 / 1690 / 4.5878E-11 / 6.83E-10
LA 3935 / 2 / 2.84 / 0.17 / 1965 / 5.8627E-23 / 3.93E-21
3 / 1.68 / 0.17 / 1690 / 5.7551E-06 / 4.54E-05
4 / 1.46 / 0.18 / 1965 / 4.6445E-05 / 0.00026108
5 / 2.23 / 0.15 / 1690 / 1.3596E-06 / 1.21E-05
6 / 1.78 / 0.28 / 263 / 5.7049E-11 / 6.95E-10
LA 3936 / 2 / 1.17 / 0.31 / 167 / 0.33375294 / 0.43845974
3 / 1.12 / 0.19 / 361 / 0.32240833 / 0.42774966
4 / 1.06 / 0.13 / 1965 / 0.59586406 / 0.67665919
5 / 1.21 / 0.19 / 338 / 0.26878594 / 0.37518037
6 / 1.21 / 0.15 / 1690 / 0.08028026 / 0.15578005
LA3937 / 2 / 1.57 / 0.23 / 263 / 0.00183299 / 0.00682279
3 / 1.68 / 0.33 / 167 / 6.4821E-06 / 4.83E-05
4 / 1.51 / 0.13 / 361 / 2.0857E-05 / 0.00013309
5 / 1.78 / 0.13 / 1965 / 0.00055937 / 0.0022714
6 / 2.28 / 0.16 / 338 / 1.6062E-15 / 3.59E-14
LA3938 / 2 / 1.45 / 0.24 / 1690 / 0.00210016 / 0.00740584
6 / 1.24 / 0.24 / 263 / 0.07860289 / 0.15578005
LA3939 / 2 / 1.11 / 0.16 / 361 / 0.39154981 / 0.48135481
6 / 1.52 / 0.16 / 1965 / 0.00031272 / 0.00135174
LA3943 / 2 / 1.46 / 0.17 / 338 / 0.00072725 / 0.00286622
6 / 1.27 / 0.21 / 1690 / 0.04869904 / 0.10525277
LA3944 / 2 / 2.69 / 0.24 / 263 / 1.8258E-16 / 5.93E-15
3 / 3.93 / 0.31 / 167 / 8.3227E-06 / 5.87E-05
4 / 1.31 / 0.14 / 361 / 0.04682249 / 0.10285596
5 / 12.44 / 0.15 / 1965 / 1.287E-06 / 1.21E-05
6 / 2.08 / 0.16 / 338 / 5.144E-11 / 6.89E-10
LA3945 / 2 / 1.2 / 0.17 / 1690 / 0.11170355 / 0.19695099
3 / 1.15 / 0.18 / 1965 / 0.2795142 / 0.38613302
4 / 1.32 / 0.18 / 1690 / 0.0060902 / 0.01813525
5 / 1.41 / 0.17 / 1965 / 0.04099452 / 0.09155443
6 / 2.07 / 0.16 / 1690 / 1.2539E-12 / 2.10E-11
LA3949 / 2 / 1.21 / 0.18 / 1965 / 0.11014107 / 0.19678538
6 / 1.18 / 0.17 / 1690 / 0.18504013 / 0.2915223
LA3952 / 2 / 0.94 / 0.73 / 263 / 0.58299783 / 0.66770692
6 / 0.97 / 0.39 / 167 / 0.78142665 / 0.83104104
LA3953 / 2 / 1.06 / 0.19 / 361 / 0.66200759 / 0.72712309
6 / 1.19 / 0.16 / 1965 / 0.30129881 / 0.40781859
LA3954 / 2 / 1.13 / 0.44 / 338 / 0.48447802 / 0.57690287
6 / 1.47 / 0.17 / 1690 / 0.02300415 / 0.05708436
LA3955 / 2 / 1.27 / 0.24 / 263 / 0.01703069 / 0.04388677
3 / 1.38 / 0.31 / 167 / 0.002394 / 0.00822554
4 / 1.1 / 0.15 / 361 / 0.38738282 / 0.48135481
5 / 0.8 / 0.15 / 1965 / 0.1870964 / 0.2915223
6 / 1.02 / 0.19 / 338 / 0.87183693 / 0.88730527
LA3956 / 2 / 1.07 / 0.17 / 1690 / 0.53032705 / 0.61261918
6 / 0.83 / 0.18 / 1965 / 0.0780034 / 0.15578005
LA3957 / 2 / 1.7 / 0.17 / 1690 / 0.01038826 / 0.02961759
LA3958 / 2 / 1.26 / 0.18 / 1965 / 0.05272089 / 0.11213651
6 / 1.28 / 0.2 / 1690 / 0.02424227 / 0.05906298
LA3961 / 2 / 1.26 / 0.24 / 1965 / 0.04080254 / 0.09155443
6 / 0.91 / 0.24 / 1690 / 0.46746159 / 0.56865264
LA3962 / 2 / 1.25 / 0.24 / 1965 / 0.05573173 / 0.11489311
6 / 1.27 / 0.15 / 1690 / 0.05491279 / 0.11489311
LA3964 / 2 / 1.05 / 0.24 / 263 / 0.61206569 / 0.68921683
3 / 1.13 / 0.16 / 361 / 0.23938992 / 0.34125797
4 / 0.93 / 0.14 / 1965 / 0.48649272 / 0.57690287
5 / 0.83 / 0.15 / 338 / 0.2463156 / 0.34743463
6 / 0.98 / 0.15 / 1690 / 0.82519679 / 0.85849564
LA3966 / 2 / 1.7 / 0.16 / 1965 / 1.245E-05 / 8.34E-05
6 / 1.44 / 0.31 / 1690 / 0.0036975 / 0.01208452
LA3967 / 2 / 1.44 / 0.17 / 1690 / 4.676E-05 / 0.00026108
3 / 1.59 / 0.16 / 1965 / 0.00028851 / 0.0012887
4 / 1.18 / 0.16 / 1690 / 0.10243231 / 0.18802643
5 / 0.93 / 0.14 / 1965 / 0.71457802 / 0.76602763
6 / 1.11 / 0.17 / 1690 / 0.22314422 / 0.3228893
LA3968 / 2 / 1.47 / 0.18 / 1965 / 0.00010361 / 0.00051422
3 / 1.20 / 0.15 / 1690 / 0.09324875 / 0.17354629
4 / 1.05 / 0.27 / 263 / 0.63556281 / 0.70971181
5 / 1.15 / 0.16 / 361 / 0.38403094 / 0.48135481
6 / 1.07 / 0.13 / 1965 / 0.47104808 / 0.56865264
LA3969 / 2 / 1.45 / 0.15 / 338 / 0.00053246 / 0.00222968
6 / 2.6 / 0.17 / 1690 / 2.2116E-16 / 5.93E-15
LA3996 / 2 / 1.21 / 0.18 / 1965 / 0.08466882 / 0.1597975
4 / 1.37 / 0.13 / 1690 / 0.00729424 / 0.02124844
6 / 1.16 / 0.28 / 263 / 0.15091833 / 0.2527882
LA4004 / 2 / 1.2 / 0.31 / 167 / 0.13077058 / 0.22757477
6 / 1.29 / 0.14 / 361 / 0.20533403 / 0.30571956
LA4005 / 2 / 2.73 / 0.13 / 1965 / 1.7335E-20 / 7.74E-19
3 / 1.92 / 0.18 / 338 / 1.6422E-07 / 1.69E-06
4 / 1.31 / 0.14 / 1690 / 0.01434658 / 0.03844883
5 / 1.08 / 0.23 / 263 / 0.69111196 / 0.74873844
6 / 1.75 / 0.16 / 361 / 2.0126E-08 / 2.25E-07
LA4006 / 2 / 1.41 / 0.13 / 1965 / 0.00518271 / 0.01602811
6 / 0.96 / 0.16 / 338 / 0.69286243 / 0.74873844
LA4008 / 2 / 1.12 / 0.16 / 1690 / 0.30475205 / 0.40836775
6 / 1.69 / 0.17 / 1965 / 3.5408E-05 / 0.00021567

Fruits of each line were harvested at different stages (2 to 6, as defined by USDA guidelines) and ethylene production ratio relative to control (LA4024) was calculated. Degree of freedom (DF), standard error (SE) raw P value calculated with the Dunnett test and false discovery rate (FDR) are reported. Details of the procedure are reported in Materials and Methods.

Table S2. Ethylene emission ratio of fruits harvested at Stage 2 between NILs and LA4024.

line / time / ratio / SE / DF / raw P / FDR
LA3913 / Day1 / 0.87 / 0.12 / 1515 / 0.083599 / 0.111
Day2 / 1.33 / 0.11 / 1526 / 4.15E-05 / 8.29E-05
Day3 / 1.5 / 0.09 / 1525 / 3.72E-11 / 1.44E-10
Day4 / 1.34 / 0.08 / 1512 / 1.94E-07 / 4.98E-07
Day5 / 1.13 / 0.07 / 1524 / 0.014806 / 0.022017
Day6 / 1.06 / 0.07 / 1511 / 0.240528 / 0.288057
Day7 / 0.9 / 0.08 / 1498 / 0.051477 / 0.070516
Day8 / 0.89 / 0.08 / 1488 / 0.037587 / 0.052753
LA3921 / Day1 / 0.54 / 0.26 / 1515 / 0.000316 / 0.00058
Day2 / 1.05 / 0.22 / 1526 / 0.75203 / 0.783365
Day3 / 1.7 / 0.18 / 1525 / 2.72E-05 / 5.57E-05
Day4 / 1.78 / 0.18 / 1512 / 3.12E-06 / 6.90E-06
Day5 / 1.76 / 0.15 / 1524 / 4.5E-08 / 1.25E-07
Day6 / 1.54 / 0.17 / 1511 / 0.000264 / 0.000494
Day7 / 1.54 / 0.24 / 1498 / 0.008096 / 0.012267
Day8 / 1.2 / 0.2 / 1488 / 0.189485 / 0.234657
LA3922 / Day1 / 2.41 / 0.33 / 1515 / 8.05E-05 / 0.000156
Day2 / 1.72 / 0.29 / 1526 / 0.00403 / 0.006396
Day3 / 1.2 / 0.24 / 1525 / 0.270521 / 0.322048
Day4 / 0.77 / 0.27 / 1512 / 0.177961 / 0.221069
Day5 / 0.73 / 0.19 / 1524 / 0.016364 / 0.023828
Day6 / 0.75 / 0.24 / 1511 / 0.083805 / 0.111
Day7 / 0.82 / 0.31 / 1498 / 0.360273 / 0.410147
Day8 / 1.13 / 0.31 / 1488 / 0.560366 / 0.597723
LA3923 / Day1 / 1.11 / 0.17 / 1515 / 0.360929 / 0.410147
Day2 / 1.33 / 0.14 / 1526 / 0.002633 / 0.004299
Day3 / 1.4 / 0.12 / 1525 / 6.05E-05 / 0.00012
Day4 / 1.44 / 0.12 / 1512 / 9.26E-06 / 1.95E-05
Day5 / 1.37 / 0.1 / 1524 / 3.2E-06 / 7.04E-06
Day6 / 1.25 / 0.1 / 1511 / 0.001842 / 0.003057
Day7 / 1.13 / 0.12 / 1498 / 0.120906 / 0.155507
Day8 / 1.06 / 0.12 / 1488 / 0.487711 / 0.533018
LA3923F1 / Day1 / 0.99 / 0.21 / 1515 / 0.931392 / 0.945575
Day2 / 1.44 / 0.19 / 1526 / 0.003044 / 0.00493
Day3 / 1.45 / 0.15 / 1525 / 0.000514 / 0.00093
Day4 / 1.25 / 0.14 / 1512 / 0.024597 / 0.035391
Day5 / 1.14 / 0.12 / 1524 / 0.142505 / 0.180386
Day6 / 1.11 / 0.12 / 1511 / 0.238042 / 0.285936
Day7 / 0.94 / 0.13 / 1498 / 0.539451 / 0.580055
Day8 / 0.93 / 0.13 / 1488 / 0.406521 / 0.458052
LA3924 / Day1 / 1.01 / 0.12 / 1515 / 0.945818 / 0.957791
Day2 / 1.3 / 0.11 / 1526 / 0.000224 / 0.000425
Day3 / 1.44 / 0.09 / 1525 / 3.45E-09 / 1.08E-08
Day4 / 1.26 / 0.08 / 1512 / 6.8E-05 / 0.000134
Day5 / 1.2 / 0.07 / 1524 / 0.000239 / 0.000449
Day6 / 1.16 / 0.07 / 1511 / 0.003815 / 0.006079
Day7 / 1.15 / 0.08 / 1498 / 0.008457 / 0.012766
Day8 / 1.16 / 0.08 / 1488 / 0.004306 / 0.006808
LA3924F1 / Day1 / 1.09 / 0.13 / 1515 / 0.309461 / 0.361943
Day2 / 1.32 / 0.11 / 1526 / 0.000217 / 0.000413
Day3 / 1.47 / 0.09 / 1525 / 4.32E-09 / 1.32E-08
Day4 / 1.38 / 0.09 / 1512 / 9.93E-08 / 2.70E-07
Day5 / 1.33 / 0.08 / 1524 / 7.88E-08 / 2.16E-07
Day6 / 1.37 / 0.08 / 1511 / 2.8E-09 / 8.90E-09
Day7 / 1.33 / 0.08 / 1498 / 6.06E-07 / 1.41E-06
Day8 / 1.23 / 0.08 / 1488 / 0.000291 / 0.000539
LA3925 / Day1 / 1.13 / 0.17 / 1515 / 0.29541 / 0.347541
Day2 / 1.02 / 0.15 / 1526 / 0.836309 / 0.864403
Day3 / 0.95 / 0.12 / 1525 / 0.538218 / 0.580055
Day4 / 0.9 / 0.11 / 1512 / 0.169343 / 0.212342
Day5 / 0.81 / 0.1 / 1524 / 0.00161 / 0.002717
Day6 / 0.88 / 0.1 / 1511 / 0.068693 / 0.093143
Day7 / 0.99 / 0.11 / 1498 / 0.854896 / 0.881336
Day8 / 1.04 / 0.1 / 1488 / 0.557356 / 0.596103
LA3926 / Day1 / 1.07 / 0.11 / 1515 / 0.34098 / 0.390808
Day2 / 1.01 / 0.1 / 1526 / 0.899704 / 0.915729
Day3 / 0.99 / 0.08 / 1525 / 0.877847 / 0.900356
Day4 / 0.96 / 0.07 / 1512 / 0.458769 / 0.511163
Day5 / 0.91 / 0.07 / 1524 / 0.03146 / 0.044467
Day6 / 0.92 / 0.07 / 1511 / 0.110009 / 0.142869
Day7 / 1 / 0.07 / 1498 / 0.994699 / 0.994699
Day8 / 1.03 / 0.07 / 1488 / 0.515852 / 0.557677
LA3927 / Day1 / 1.61 / 0.11 / 1515 / 4.55E-10 / 1.52E-09
Day2 / 1.63 / 0.1 / 1526 / 3.41E-14 / 1.66E-13
Day3 / 1.39 / 0.08 / 1525 / 5.05E-09 / 1.53E-08
Day4 / 1.34 / 0.07 / 1512 / 2.32E-08 / 6.63E-08
Day5 / 1.27 / 0.07 / 1524 / 2.3E-07 / 5.79E-07
Day6 / 1.4 / 0.07 / 1511 / 2.74E-13 / 1.23E-12
Day7 / 1.58 / 0.07 / 1498 / 2.95E-20 / 2.62E-19
Day8 / 1.89 / 0.07 / 1488 / 5.79E-38 / 1.78E-36
LA3932 / Day1 / 1.01 / 0.11 / 1515 / 0.889955 / 0.91044
Day2 / 1.27 / 0.1 / 1526 / 0.000234 / 0.000442
Day3 / 1.35 / 0.08 / 1525 / 1.6E-07 / 4.20E-07
Day4 / 1.39 / 0.08 / 1512 / 8.09E-10 / 2.65E-09
Day5 / 1.21 / 0.07 / 1524 / 3.09E-05 / 6.27E-05
Day6 / 1.16 / 0.07 / 1511 / 0.001418 / 0.002435
Day7 / 1.03 / 0.07 / 1498 / 0.545791 / 0.585299
Day8 / 0.87 / 0.07 / 1488 / 0.005267 / 0.008198
LA3934 / Day1 / 0.63 / 0.33 / 1515 / 0.041188 / 0.057404
Day2 / 0.79 / 0.29 / 1526 / 0.210498 / 0.258279
Day3 / 0.94 / 0.26 / 1525 / 0.722265 / 0.756298
Day4 / 1.18 / 0.22 / 1512 / 0.291912 / 0.344439
Day5 / 1.42 / 0.21 / 1524 / 0.016382 / 0.023828
Day6 / 1.57 / 0.19 / 1511 / 0.000807 / 0.001428
Day7 / 1.42 / 0.26 / 1498 / 0.058102 / 0.07932
Day8 / 1.67 / 0.26 / 1488 / 0.004526 / 0.007128
LA3935 / Day1 / 1.69 / 0.29 / 1515 / 0.007609 / 0.011573
Day2 / 1.92 / 0.23 / 1526 / 1.63E-05 / 3.37E-05
Day3 / 1.89 / 0.19 / 1525 / 1.38E-06 / 3.16E-06
Day4 / 2.11 / 0.18 / 1512 / 7.63E-09 / 2.28E-08
Day5 / 2.27 / 0.15 / 1524 / 2.94E-14 / 1.45E-13
Day6 / 2.21 / 0.16 / 1511 / 2.68E-12 / 1.12E-11
Day7 / 1.92 / 0.18 / 1498 / 4.15E-07 / 9.83E-07
Day8 / 2.08 / 0.18 / 1488 / 6.91E-09 / 2.08E-08
LA3936 / Day1 / 0.76 / 0.28 / 1515 / 0.148745 / 0.187691
Day2 / 1.15 / 0.21 / 1526 / 0.311842 / 0.363665
Day3 / 1.49 / 0.18 / 1525 / 0.001047 / 0.001821
Day4 / 1.86 / 0.18 / 1512 / 1.55E-06 / 3.51E-06
Day5 / 1.73 / 0.15 / 1524 / 1.18E-07 / 3.18E-07
Day6 / 1.79 / 0.16 / 1511 / 2.37E-07 / 5.92E-07
Day7 / 1.66 / 0.17 / 1498 / 1.34E-05 / 2.80E-05
Day8 / 1.88 / 0.2 / 1488 / 5.22E-06 / 1.13E-05
LA3937 / Day1 / 1.15 / 0.31 / 1515 / 0.506368 / 0.5504
Day2 / 1.88 / 0.25 / 1526 / 0.000141 / 0.000271
Day3 / 1.93 / 0.21 / 1525 / 4.72E-06 / 1.03E-05
Day4 / 1.85 / 0.19 / 1512 / 5.56E-06 / 1.19E-05
Day5 / 1.72 / 0.18 / 1524 / 1.43E-05 / 2.98E-05
Day6 / 1.98 / 0.17 / 1511 / 9.17E-09 / 2.72E-08
Day7 / 1.76 / 0.19 / 1498 / 2.28E-05 / 4.70E-05
Day8 / 1.95 / 0.19 / 1488 / 4.01E-07 / 9.60E-07
LA3939 / Day1 / 0.73 / 0.12 / 1515 / 7.99E-05 / 0.000156
Day2 / 0.95 / 0.1 / 1526 / 0.473565 / 0.523276
Day3 / 1.18 / 0.09 / 1525 / 0.005202 / 0.008128
Day4 / 1.3 / 0.08 / 1512 / 2.31E-06 / 5.13E-06
Day5 / 1.26 / 0.07 / 1524 / 1.49E-06 / 3.38E-06
Day6 / 1.28 / 0.07 / 1511 / 2.18E-07 / 5.52E-07
Day7 / 1.31 / 0.07 / 1498 / 1.52E-07 / 4.03E-07
Day8 / 1.35 / 0.07 / 1488 / 3.64E-09 / 1.12E-08
LA3943 / Day1 / 0.9 / 0.12 / 1515 / 0.164032 / 0.20633
Day2 / 0.99 / 0.1 / 1526 / 0.895337 / 0.91361
Day3 / 1.06 / 0.08 / 1525 / 0.332564 / 0.383359
Day4 / 1.14 / 0.08 / 1512 / 0.015558 / 0.022963
Day5 / 1.1 / 0.07 / 1524 / 0.046601 / 0.0645
Day6 / 1.18 / 0.07 / 1511 / 0.000631 / 0.001131
Day7 / 1.3 / 0.07 / 1498 / 5.06E-07 / 1.19E-06
Day8 / 1.45 / 0.07 / 1488 / 4.36E-13 / 1.90E-12
LA3944 / Day1 / 2.63 / 0.19 / 1515 / 6.61E-14 / 3.15E-13
Day2 / 3.28 / 0.16 / 1526 / 9.46E-27 / 1.65E-25
Day3 / 3.38 / 0.14 / 1525 / 3.33E-36 / 8.89E-35
Day4 / 3.25 / 0.13 / 1512 / 1.1E-38 / 3.67E-37
Day5 / 2.9 / 0.11 / 1524 / 4.13E-41 / 1.65E-39
Day6 / 2.57 / 0.11 / 1511 / 6.11E-33 / 1.44E-31
Day7 / 2.47 / 0.12 / 1498 / 1.9E-26 / 3.17E-25
Day8 / 2.45 / 0.12 / 1488 / 3.6E-25 / 5.14E-24
LA3944F1 / Day1 / 1.28 / 0.22 / 1515 / 0.098698 / 0.12944
Day2 / 1.58 / 0.19 / 1526 / 0.000296 / 0.000545
Day3 / 1.57 / 0.16 / 1525 / 3.62E-05 / 7.31E-05
Day4 / 1.63 / 0.19 / 1512 / 1.6E-06 / 3.60E-06
Day5 / 1.61 / 0.13 / 1524 / 1.23E-07 / 3.30E-07
Day6 / 1.6 / 0.13 / 1511 / 1.94E-07 / 4.98E-07
Day7 / 1.85 / 0.14 / 1498 / 2.46E-10 / 8.55E-10
Day8 / 1.85 / 0.14 / 1488 / 1.88E-10 / 6.73E-10
LA3945 / Day1 / 2.12 / 0.25 / 1515 / 6.6E-06 / 1.40E-05
Day2 / 2.04 / 0.21 / 1526 / 1.75E-07 / 4.55E-07
Day3 / 2.4 / 0.17 / 1525 / 1.98E-13 / 9.22E-13
Day4 / 2.8 / 0.16 / 1512 / 4.36E-19 / 3.17E-18
Day5 / 2.8 / 0.14 / 1524 / 7.45E-26 / 1.10E-24
Day6 / 2.78 / 0.14 / 1511 / 9.12E-24 / 1.14E-22
Day7 / 2.23 / 0.16 / 1498 / 2.18E-13 / 1.00E-12
Day8 / 2.73 / 0.16 / 1488 / 1.41E-19 / 1.11E-18
LA3945F1 / Day1 / 0.86 / 0.15 / 1515 / 0.136939 / 0.173891
Day2 / 1.04 / 0.13 / 1526 / 0.660672 / 0.697279
Day3 / 1.16 / 0.11 / 1525 / 0.040396 / 0.056498
Day4 / 1.23 / 0.1 / 1512 / 0.002686 / 0.004367
Day5 / 1.28 / 0.09 / 1524 / 4.57E-05 / 9.10E-05
Day6 / 1.36 / 0.09 / 1511 / 4.15E-07 / 9.83E-07
Day7 / 1.37 / 0.09 / 1498 / 1.64E-06 / 3.67E-06
Day8 / 1.3 / 0.1 / 1488 / 6.98E-05 / 0.000137
LA3952 / Day1 / 0.74 / 0.13 / 1515 / 0.000434 / 0.000789
Day2 / 0.76 / 0.11 / 1526 / 0.000177 / 0.000339
Day3 / 0.81 / 0.09 / 1525 / 0.001253 / 0.002161
Day4 / 0.85 / 0.08 / 1512 / 0.00592 / 0.009179
Day5 / 0.85 / 0.07 / 1524 / 0.001965 / 0.003248
Day6 / 0.86 / 0.07 / 1511 / 0.003152 / 0.005063
Day7 / 0.9 / 0.08 / 1498 / 0.07015 / 0.094798
Day8 / 1 / 0.08 / 1488 / 0.970847 / 0.979972
LA3953 / Day1 / 0.94 / 0.11 / 1515 / 0.355656 / 0.406465
Day2 / 1.02 / 0.09 / 1526 / 0.787316 / 0.815871
Day3 / 1.06 / 0.08 / 1525 / 0.306409 / 0.359424
Day4 / 1 / 0.07 / 1512 / 0.99134 / 0.994699
Day5 / 0.93 / 0.06 / 1524 / 0.101354 / 0.132057
Day6 / 0.89 / 0.06 / 1511 / 0.006208 / 0.009588
Day7 / 0.87 / 0.07 / 1498 / 0.001783 / 0.002971
Day8 / 0.93 / 0.07 / 1488 / 0.095809 / 0.126064
LA3954 / Day1 / 0.86 / 0.1 / 1515 / 0.025662 / 0.036792
Day2 / 1 / 0.09 / 1526 / 0.485533 / 0.533018
Day3 / 1.10 / 0.08 / 1525 / 0.061793 / 0.084071
Day4 / 1.06 / 0.07 / 1512 / 0.223819 / 0.272121
Day5 / 1.05 / 0.061 / 1524 / 0.237187 / 0.285767
Day6 / 1.07 / 0.06 / 1511 / 0.121881 / 0.156258
Day7 / 1.04 / 0.07 / 1498 / 0.395564 / 0.446965
Day8 / 0.99 / 0.06 / 1488 / 0.771081 / 0.801123
LA3955 / Day1 / 1.18 / 0.31 / 1515 / 0.422312 / 0.474507
Day2 / 1.48 / 0.27 / 1526 / 0.026076 / 0.037252
Day3 / 1.41 / 0.21 / 1525 / 0.016064 / 0.023624
Day4 / 1.14 / 0.19 / 1512 / 0.328077 / 0.380379
Day5 / 1.09 / 0.17 / 1524 / 0.451223 / 0.504159
Day6 / 1.21 / 0.17 / 1511 / 0.099547 / 0.130127
Day7 / 1.05 / 0.20 / 1498 / 0.710954 / 0.746409
Day8 / 1.12 / 0.23 / 1488 / 0.471734 / 0.522697
LA3958 / Day1 / 0.75 / 0.24 / 1515 / 0.07545 / 0.101616
Day2 / 0.94 / 0.21 / 1526 / 0.632136 / 0.670701
Day3 / 1.15 / 0.17 / 1525 / 0.227359 / 0.274755
Day4 / 1.10 / 0.16 / 1512 / 0.37755 / 0.427818
Day5 / 1.34 / 0.14 / 1524 / 0.002612 / 0.004282
Day6 / 1.39 / 0.14 / 1511 / 0.000868 / 0.001523
Day7 / 1.26 / 0.17 / 1498 / 0.046072 / 0.063988
Day8 / 1.34 / 0.17 / 1488 / 0.014996 / 0.022217
LA3961 / Day1 / 1.11 / 0.13 / 1515 / 0.214157 / 0.261966
Day2 / 0.95 / 0.11 / 1526 / 0.515772 / 0.557677
Day3 / 0.93 / 0.09 / 1525 / 0.273616 / 0.324767
Day4 / 0.96 / 0.09 / 1512 / 0.505735 / 0.5504
Day5 / 0.98 / 0.08 / 1524 / 0.65178 / 0.689714
Day6 / 1.15 / 0.08 / 1511 / 0.009418 / 0.014163
Day7 / 1.28 / 0.08 / 1498 / 9.28E-06 / 1.95E-05
Day8 / 1.32 / 0.08 / 1488 / 8.46E-07 / 1.96E-06
LA3962 / Day1 / 1.03 / 0.12 / 1515 / 0.710195 / 0.746409
Day2 / 1.05 / 0.10 / 1526 / 0.451106 / 0.504159
Day3 / 1.09 / 0.08 / 1525 / 0.125966 / 0.160979
Day4 / 1.19 / 0.08 / 1512 / 0.001519 / 0.002575
Day5 / 1.28 / 0.07 / 1524 / 2.99E-07 / 7.39E-07
Day6 / 1.5 / 0.07 / 1511 / 2.44E-19 / 1.84E-18
Day7 / 1.74 / 0.07 / 1498 / 3.44E-26 / 5.50E-25
Day8 / 1.94 / 0.07 / 1488 / 4.72E-37 / 1.35E-35
LA3964 / Day1 / 0.86 / 0.14 / 1515 / 0.114991 / 0.148375
Day2 / 0.85 / 0.12 / 1526 / 0.048413 / 0.066547
Day3 / 0.83 / 0.1 / 1525 / 0.007062 / 0.010824
Day4 / 0.81 / 0.09 / 1512 / 0.001731 / 0.002897
Day5 / 0.82 / 0.08 / 1524 / 0.000672 / 0.0012
Day6 / 0.83 / 0.08 / 1511 / 0.001695 / 0.002848
Day7 / 0.85 / 0.09 / 1498 / 0.010638 / 0.015877
Day8 / 0.83 / 0.09 / 1488 / 0.003476 / 0.005561
LA3965 / Day1 / 0.97 / 0.12 / 1515 / 0.734341 / 0.766936
Day2 / 0.86 / 0.1 / 1526 / 0.026774 / 0.038113
Day3 / 0.94 / 0.09 / 1525 / 0.337591 / 0.388036
Day4 / 0.95 / 0.08 / 1512 / 0.316689 / 0.368243
Day5 / 1.05 / 0.07 / 1524 / 0.282995 / 0.334906
Day6 / 1.29 / 0.07 / 1511 / 2.05E-07 / 5.23E-07
Day7 / 1.42 / 0.07 / 1498 / 2.99E-11 / 1.17E-10
Day8 / 1.61 / 0.07 / 1488 / 7.61E-20 / 6.47E-19
LA3966 / Day1 / 1.39 / 0.1 / 1515 / 3.77E-07 / 9.15E-07
Day2 / 1.35 / 0.08 / 1526 / 4.18E-08 / 1.17E-07
Day3 / 1.38 / 0.07 / 1525 / 1.57E-11 / 6.20E-11
Day4 / 1.43 / 0.06 / 1512 / 8.51E-16 / 4.73E-15
Day5 / 1.45 / 0.06 / 1524 / 1.13E-21 / 1.13E-20
Day6 / 1.7 / 0.06 / 1511 / 1.98E-40 / 7.20E-39
Day7 / 1.87 / 0.06 / 1498 / 1.23E-47 / 5.47E-46
Day8 / 2.08 / 0.06 / 1488 / 6.78E-65 / 6.78E-63
LA3967 / Day1 / 1.27 / 0.11 / 1515 / 0.001058 / 0.001831
Day2 / 1.45 / 0.09 / 1526 / 3.64E-09 / 1.12E-08
Day3 / 1.6 / 0.08 / 1525 / 1.01E-17 / 6.61E-17
Day4 / 1.63 / 0.07 / 1512 / 1.84E-21 / 1.80E-20
Day5 / 1.57 / 0.06 / 1524 / 1.19E-23 / 1.45E-22
Day6 / 1.66 / 0.06 / 1511 / 3.81E-29 / 8.47E-28
Day7 / 1.57 / 0.07 / 1498 / 2.95E-20 / 2.62E-19
Day8 / 1.59 / 0.07 / 1488 / 9.85E-22 / 1.04E-20
LA3967F1 / Day1 / 0.59 / 0.4 / 1515 / 0.048232 / 0.066527
Day2 / 1 / 0.35 / 1526 / 0.994136 / 0.994699
Day3 / 1.11 / 0.29 / 1525 / 0.608043 / 0.646854
Day4 / 1.27 / 0.27 / 1512 / 0.193617 / 0.239033
Day5 / 1.2 / 0.23 / 1524 / 0.248702 / 0.296958
Day6 / 1.33 / 0.23 / 1511 / 0.07583 / 0.101785
Day7 / 1.36 / 0.25 / 1498 / 0.077697 / 0.103943
Day8 / 1.31 / 0.25 / 1488 / 0.113591 / 0.147043
LA3968 / Day1 / 0.79 / 0.26 / 1515 / 0.173105 / 0.215707
Day2 / 1 / 0.22 / 1526 / 0.972623 / 0.979972
Day3 / 1.4 / 0.18 / 1525 / 0.007562 / 0.011545
Day4 / 1.58 / 0.18 / 1512 / 0.000357 / 0.000653
Day5 / 1.39 / 0.15 / 1524 / 0.001469 / 0.002512
Day6 / 1.36 / 0.16 / 1511 / 0.006669 / 0.01026
Day7 / 1.11 / 0.22 / 1498 / 0.486381 / 0.533018
Day8 / 1.18 / 0.2 / 1488 / 0.226756 / 0.274755
LA3969 / Day1 / 0.9 / 0.1 / 1515 / 0.127098 / 0.161909
Day2 / 1.08 / 0.09 / 1526 / 0.206994 / 0.254762
Day3 / 1.16 / 0.07 / 1525 / 0.002483 / 0.004088
Day4 / 1.07 / 0.07 / 1512 / 0.171111 / 0.213889
Day5 / 1.03 / 0.06 / 1524 / 0.478586 / 0.527367
Day6 / 1.16 / 0.06 / 1511 / 0.00029 / 0.000539
Day7 / 1.34 / 0.06 / 1498 / 6.74E-11 / 2.50E-10
Day8 / 1.58 / 0.06 / 1488 / 8.08E-25 / 1.12E-23
LA3996 / Day1 / 1.16 / 0.1 / 1515 / 0.030133 / 0.042742
Day2 / 1.38 / 0.09 / 1526 / 3.17E-08 / 9.00E-08
Day3 / 1.59 / 0.07 / 1525 / 1.25E-19 / 1.02E-18
Day4 / 1.7 / 0.07 / 1512 / 1.92E-28 / 3.66E-27
Day5 / 1.58 / 0.06 / 1524 / 2.54E-27 / 4.61E-26
Day6 / 1.59 / 0.06 / 1511 / 1.07E-28 / 2.13E-27
Day7 / 1.54 / 0.06 / 1498 / 7.07E-22 / 7.86E-21
Day8 / 1.55 / 0.06 / 1488 / 8.04E-23 / 9.46E-22
LA4005 / Day1 / 2.67 / 0.12 / 1515 / 2.99E-33 / 7.48E-32
Day2 / 2.76 / 0.1 / 1526 / 4.44E-48 / 2.22E-46
Day3 / 3.02 / 0.08 / 1525 / 7.91E-72 / 1.05E-69
Day4 / 2.88 / 0.08 / 1512 / 1.56E-75 / 6.23E-73
Day5 / 2.51 / 0.07 / 1524 / 8.71E-75 / 1.74E-72
Day6 / 2.33 / 0.07 / 1511 / 2.61E-64 / 2.09E-62
Day7 / 2.21 / 0.07 / 1498 / 8.68E-50 / 4.96E-48
Day8 / 2.38 / 0.07 / 1488 / 1.34E-59 / 8.93E-58
LA4005-1 / Day1 / 1.87 / 0.18 / 1515 / 1.75E-07 / 4.55E-07
Day2 / 1.86 / 0.15 / 1526 / 1E-09 / 3.26E-09
Day3 / 2.02 / 0.17 / 1525 / 2.33E-15 / 1.26E-14
Day4 / 2.11 / 0.12 / 1512 / 1.3E-19 / 1.04E-18
Day5 / 1.89 / 0.1 / 1524 / 2.11E-18 / 1.45E-17
Day6 / 1.83 / 0.1 / 1511 / 5.06E-17 / 3.11E-16
Day7 / 1.71 / 0.11 / 1498 / 4.46E-12 / 1.80E-11
Day8 / 1.62 / 0.11 / 1488 / 3E-10 / 1.02E-09
LA4005-2 / Day1 / 2.01 / 0.19 / 1515 / 2.6E-07 / 6.45E-07
Day2 / 2.31 / 0.17 / 1526 / 5.61E-13 / 2.41E-12
Day3 / 2.36 / 0.14 / 1525 / 2.04E-17 / 1.30E-16
Day4 / 2.31 / 0.13 / 1512 / 4.18E-19 / 3.10E-18
Day5 / 2.13 / 0.11 / 1524 / 7.15E-20 / 6.22E-19
Day6 / 2.02 / 0.11 / 1511 / 1.11E-17 / 7.15E-17
Day7 / 1.99 / 0.12 / 1498 / 8.57E-15 / 4.40E-14
Day8 / 1.95 / 0.12 / 1488 / 1.72E-14 / 8.61E-14
LA4005-3 / Day1 / 1.3 / 0.16 / 1515 / 0.016271 / 0.023828
Day2 / 1.6 / 0.14 / 1526 / 3.59E-07 / 8.80E-07
Day3 / 1.8 / 0.12 / 1525 / 6.25E-14 / 3.01E-13
Day4 / 1.61 / 0.11 / 1512 / 1.61E-10 / 5.84E-10
Day5 / 1.47 / 0.09 / 1524 / 3.06E-09 / 9.63E-09
Day6 / 1.73 / 0.09 / 1511 / 5.85E-17 / 3.55E-16
Day7 / 1.79 / 0.1 / 1498 / 1.49E-16 / 8.92E-16
Day8 / 1.96 / 0.1 / 1488 / 1.13E-21 / 1.13E-20
LA4005-4 / Day1 / 1.83 / 0.18 / 1515 / 3.93E-07 / 9.47E-07
Day2 / 1.89 / 0.15 / 1526 / 4.01E-10 / 1.35E-09
Day3 / 1.91 / 0.13 / 1525 / 3.04E-13 / 1.35E-12
Day4 / 1.77 / 0.12 / 1512 / 3.93E-12 / 1.61E-11
Day5 / 1.5 / 0.1 / 1524 / 1.82E-08 / 5.23E-08
Day6 / 1.4 / 0.1 / 1511 / 3.39E-06 / 7.40E-06
Day7 / 1.4 / 0.11 / 1498 / 6.52E-06 / 1.39E-05
Day8 / 1.35 / 0.11 / 1488 / 9.54E-05 / 0.000184
LA4005F1 / Day1 / 0.98 / 0.18 / 1515 / 0.870019 / 0.894621
Day2 / 1.26 / 0.15 / 1526 / 0.019938 / 0.028896
Day3 / 1.34 / 0.13 / 1525 / 0.000718 / 0.001277
Day4 / 1.5 / 0.12 / 1512 / 5.84E-07 / 1.37E-06
Day5 / 1.54 / 0.1 / 1524 / 1.77E-09 / 5.65E-09
Day6 / 1.6 / 0.1 / 1511 / 4.91E-11 / 1.87E-10
Day7 / 1.65 / 0.11 / 1498 / 8.43E-11 / 3.10E-10
Day8 / 1.758 / 0.11 / 1488 / 4.32E-13 / 1.90E-12
LA4008 / Day1 / 1.07 / 0.11 / 1515 / 0.33049 / 0.38207
Day2 / 1.19 / 0.09 / 1526 / 0.005169 / 0.008109
Day3 / 1.04 / 0.08 / 1525 / 0.464742 / 0.51638
Day4 / 0.86 / 0.07 / 1512 / 0.001515 / 0.002575
Day5 / 0.7 / 0.06 / 1524 / 5.01E-16 / 2.86E-15
Day6 / 0.7 / 0.06 / 1511 / 1.82E-16 / 1.07E-15
Day7 / 0.65 / 0.07 / 1498 / 1.9E-19 / 1.46E-18
Day8 / 0.59 / 0.07 / 1488 / 1.06E-28 / 2.13E-27

Fruits of NILs were harvested at breaker (S2 and day1) and ethylene production monitored daily (Day 1 to Day 8). Ethylene production is expressed as the ratio of NIL to control (LA4024). Raw P value, SE (standard error), degree of freedom (DF) and false discovery rate (FDR) are also presented. The designation F1 after the line name indicates the F1 progeny obtained from a cross with LA4024.

Table S3. List of CAPS markers used.

Chr. / cM / Name / Origin / Sequence / ToC / length / Enz. / LA4024 / LA1777
1 / 87 / cLED-15-I5F / D / GGACCGTGTCTACCTTAGTTCTCCTA / 58 / 400 / Sml I / 400 / 200
cLED-15-I5R / CTCTACACGACCGGTCCATTCCT
95 / C2_At3g04710F / W / AGGGTGCAGATCCTGCAATACCCAG / 58 / 1300 / Dpn II / 800 +200 / 1300
C2_At3g04710R / TCCAGCCTCACTTTGTAAATCAACATC
160 / C2_At4g34700F / W / TGAAGCATCCTACAATAAGTGGCG / 55 / 1100 / Alu I / 700 / 600
C2_At4g34700R / TCTGTTGAACTTGGAACCACCAGG
2 / 68 / C2_At4g21580F / W / TCGCTGGAAAATTGGTGATCAG / 55 / 1350 / HaeIII / 750+ 570 / 1350
C2_At4g21580R / ATGAAAATGGTCGACCAAACAGTG
69.5 / C2_T1585F / D / TAAATCTGCTCTGTCCCAAG / 52 / 1000 / Xho I / 800 / 840
C2_T1585R / CCGTTAGGATTCACATCAAG
87.5 / C2_At5g66530F / W / TTCAGGAATGGCATTTGCAAGTGTG / 55 / 900 / Apo I / 350 / 600
C2_At5g66530R / ACCATTGAATACAGCATCTGGTCGAAC
89.5 / KFG OVATEF / GTGGGGTGGTGGAGTTAGAAGTC / 58 / 350 / NO / 300 / 350
KFG OVATER / GTTTTCTGTCGTTGAACGGAAAGC
112 / C2_At3g02300F / W / ATGGATATTGATGAAATACTTGGGG / 55 / 1100 / Apo I / 100 / 90
C2_At3g02300R / TCACTTTCTTGGGGTGCTTCCTAGC
113 / C2_At1g19690F / D / GGTCAGAAGATGAGATTCTCCA / 55 / 500 / Kpn I / 200 / 300
C2_At1g19690R / CTGGCCATTTCTCCTCAACC
143 / C2_At1g67730F / W / TGGGATTGATGTGCAATGCCAGG / 60 / 800 / Bgl II / 700 / 600
C2_At1g67730R / AGGGCAGCCCGAGCATAACC
3 / 75.7 / C2_At5g51110F / W / TGGTGGAAGGCACAGGGCAC / 55 / 360 / no / 320 / 400
C2_At5g51110R / TCTTTACTTGATCTATTTTAGCAGC
168 / C2_At5g49830F / W / ACGCTACTTATCTAGAAATTTGCTTC / 55 / 450 / EcoRI / 450 / 400
C2_At5g49830R / TCAATAACTCCCATGAGATCTGACAG
4 / 0 / T0707F / W / TCGTGGATTATGGGCTTCTT / 58 / 458 / Dde I / 458 / 400
T0707R / GGTAAGGCTGCAACACATCA
82.3 / C2_At4g09040 / W / ACAATGGGCTCACATGAGGAAGC / 54 / 350 / AluI / 350 / 250
C2_At4g09040 / TATCATTGAATATGACTTCAGCAGAAAC
82.5 / C2 At4g09010F / W / TAAGGGGCTTGATGCTGCTTTG / 56 / 587 / Alu I / 359+ 132 / 358+ 152
C2 At4g09010R / TAAAGGTCGATTTGACTGCACTTTG
85.5 / T1600F / D / GATTTGGAGAAGGGGGCTATTGCA / 58 / 750 / Apo I / 180 +500 / 300
T1600R / AGGGTTGTCAGGTGCCAATGC
88.3 / C2_At1g27530F / D / GCTCACTCTCTCCAACACT / 58 / 650 / Alu I / 180 +400 / 150 +420
C2_At1g27530R / CTGAGTTCACAACTGAAACC
102 / C2_At1g46480F / W / TAAGAGTTTCATTAGGCCTGAAAG / 55 / 500 / Apo I / 190 / 220
C2_At1g46480R / TATCTGTTCTTGTGTTGGATTCC
102 / SSR146 F / W / TATGGCCATGGCTGAACC / 55 / 250 / no / 250 / 230
SSR146 R / CGAACGCCACCACTATACCT
121 / C2_At1g75350F / D / GCTCACTCTCTCCAACACT / 55 / 600 / Mse I / 400 / 300
C2_At1g75350R / CTGAGTTCACAACTGAAACC
121 / P33F / D / CAAGAGCAGCTAAGGAAGCTAAAGG / 58 / 300 / Apo I / 300 / 150
P33R / GCTTCAGAGAATTGTCCACA
136 / C4_ TG498F / W / CAGCTGGAGCTCACATCACAA / 55 / 400 / no / 375 / 400
C4_TG498R / GTGGAGAAAGGAGGGAGAAAAGT
5 / 22 / Bs4F / W / GGAGCTGAATACGGATTGGA / 55 / 1111 / Ase I / 1000 / 800 +200
Bs4R / ATCGTTCCGATGATTTCTGG
30 / SGN-E343939F C05HBa0168M18.1 / D / TAGTGGCAGATTGATCAGCA / 53 / 800 / Bgl I / 800 / 600 +200
SGN-E343939R C05HBa0168M18.1 / TCTTTTGCCAGAGGAAATCG
51 / C2_At4g24830F / W / ATACTTGCTTGGGACATCAATGGC / 55 / 1400 / Msp I / 200 +1200 / 1400
C2_At4g24830R / TCCAACCTCTCTGGCAACATCAACC
6 / 44 / C2_At1g52200 F / W / ACATTTGGACAAATAGCAGAAGTC / 55 / 221 / Sph I / 180 / 200
C2_At1g52200 R / TGAGAGCAGACAGCAGGCATCATC
46 / C2_At1g22850F / W / ATCATTGTTTCCATTGGTGGAACG / 54 / 200 / Rsa I / 100 / 200
C2_At1g22850R / TGCAAGAAATTTCTTGTTCCCTTC
48 / C2_At1g73885F / D / ACAATGGCTCTCACAGGAAATCGA / 58 / 300 / Mwo I / 150 / 300
C2_At1g73885R / TGTGGTTGCAGAAACCCTATCTTG
50 / TG365F / D / CTTTGGGGCCACAATCGTA / 55 / 416 / Mwo I / 400 / 350
TG365R / TGTCATATTTCTCCTCCGAGCT
55.5 / C2_At5g62530 F / W / TTCGACTGGAAGATCCTTGGG / 55 / 200 / DraI / 100 / 200
C2_At5g62530 R / TGATTCAGCTGAACACTTCTGACC
56 / C2_At5g07960F / W / AAGATCTTCCTATAGATTACTCCG / 54 / 450 / Bgl II / 300 / 400
C2_At5g07960R / TGAATATAATAGCAAGCCACGAGC
57 / T1639F / D / GTTTTCTCTTCAGCAATGGCAGCA / 58 / 600 / Alu I / 180 +220 / 350
T1639R / CCACACAATTCACCGTTATTCCTGTC
59 / T1556F / D / ATCTCCAGGCATGTATAGGCGTTC / 58 / 500 / Hae III / 180 +220 / 200
T1556R / TTTCATGCTATTGCTCGGAGCTGA
62.5 / C2_At4g24690F / W / TTGCCTTCAGGGGAGAAGTTTGG / 54 / 350 / Apo I / 320 / 350
C2At4g24690R / TCAACTAATTCCATGGCAGGGTTTG
62.7 / C2_At1g24360F / W / TCCGGTTGTTATTGTCACTGGAGC / 55 / 720 / Dde I / 350 / 580
C2_At1g24360R / TGGAAACTTCTTCTGCCTCCTTTG
64.5 / C2_At1g12060F / D / CAGAGTGTGGATGTAGAAACTCAAGC / 57 / 360 / DpnII / 200+ 180 / 360
C2_At1g12060R / GTGGCACAGTTCAGTCATCATTCTC
67.3 / C2_At1g09340F / W / ATCCTGTTGAAGAGTGGTTCTTCC / 56 / 500 / MslI / 400 / 600
C2_At1g09340R / TGGCGCAAGCCTTAGCTAATC
69 / C2_At4g34215F / W / TACTCTGGGCAAAGCAACATGG / 55 / 500 / Mwo I / 350+ 150 / 500
C2_At4g34215R / ATGGCAGATGCAAATCAGCACG
72 / T1049F / D / AGAAGGCGTGGATGGTTGGTGT / 60 / 710 / Bcl I / 580 / 300 +310
T1049R / ATCATCCTTTGCCCATCTTCAGGA
73 / C2_At1g65700F / D / AGCCAGCAAATTGTTCAAGC / 56 / 2000 / Apo I / 600 +800 / 500 + 850
C2_At1g65700R / CAGTTTGTGTTCCTAGCTAC
74 / C2_At5g22620F / D / CGGCGATAAAGGGGGCGAAG / 56 / 600 / Apo I / 400+ 200 / 600
C2_At5g22620R / GGTCAATTTCCCTCATGTCAGA
74.5 / B F / W / CCCTTCACCACTCTCCATGT / 60 / 1021 / Hae III / 300+ 500 / 800
B R / AGAAAGGAACCCTTGCCAGT
8 / 15 / T0680F / D / TTGCTGATGAGGGCTGCCAGT / 55 / 1400 / Dde I / 300 +1000 / 200 +600
T0680R / TGACCAACTCTCAAAGCATGCAGT
30.1 / C2_At2g26830F / W / TCAAATCTAGATGGTTCTCACTTCTCTG / 55 / 1500 / Taq I / 1500 / 1300+ 200
C2_At2g26830R / AAGTGCGTGCATCAATAAATGACTG
82 / C2_At4g23840F / W / AGGGGAGAGAGTTATGTGGATGCAG / 57 / 400 / Apo I / 400 / 380
C2_At4g23840R / ACCATCAGCTGTGACACCTGTTTCTG
87 / TG294F / D / ATAAGAGGCTCGTTGAGCTT / 57 / 600 / Nco I / 600 / 400+ 200
TG294R / CATTGTAATTGCTGGCTGAG
10 / 31.1 / TG560F / D / AGAGATGCTGGGGATTGAATATCC / 55 / 400 / Bcl I / 200 / 400
TG560R / TAGGTCAAAACATCCCCAAGTAGC
41.5 / U241700F / W / GAGAATCTCGGGTTTTCGTTAG / 55 / 520 / Dra I / 520 / 300 +200
U241700R / CAAGAAATCTTCCAACTCTTTCGAC
61 / C2_At3g58470F / ATTGCTTGTCCCACACTTTATGC / 55 / 1000 / Hinf I / 1000 / 1200
C2_At3g58470R / TACTGTTCAAACCGTTTGTCATACTC
71.5 / C2_At3g09740F / D / CACGTCTCTATTCTTCTGTTGA / 50 / 2000 / Apo I / 1000 / 500
C2_At3g09740R / CATCTGGTATGGCATTTATTCT
78 / T1521F / D / CAAGTTCTTCAGCCTCCGTGATC / 58 / 900 / no / 800 / 900
T1521R / GTCTTCTGATGTGTTGTGCGCTA
81 / U221455 F / W / AGGCGCTTCTTATTATCTTTCTC / 55 / 870 / no / 750 / 870
U221455 R / ACCACAAGCAATCATTTCTACACC
86 / TG233F / W / CATGCCTTTTTCTTGGGATG / 52 / 300 / Hinc II / 280 / 300
TG233R / TGGAACCCCTTTAACTGTGC
11 / 1 / TG497F / W / CGGAGAGTGAAGATGCATTG / 57 / 900 / Taq I / 500 / 400
TG497R / AAGTTCCAGAGGGAGCACAA
12 / 0 / TG180 F / W / TCTCAGTGGACTAAGGGGTCA / 55 / 1000 / AluI / 580+ 230 / 700+ 300
TG180 R / TCACAGCAGACATGTCGGAC
34 / C2_At2g06005 F / W / AGATGGCGTTGTTGCAATATCAGC / 55 / 400 / Sml I / 450 / 300+ 230
C2_At2g06005 R / TCATTACATCTTTCGTATTTGCTCAAG
54.5 / C12_At5g42740 F / W / AGCACCATTTGAGAAAAATATACCTG / 55 / 1000 / ApoI / 1000 / 765+ 230
C12_At5g42740 R / ATCCAAGGAATGAAACATTCCACAC
59 / C12_At4g18593F / W / AGGTGATTGTTATAATCGTGGAGAAAG / 55 / 850 / HaeIII / 700+ 150 / 850
C12_At4g18593R / TTCACAATGCGCACATAAAAGCTTG
115 / C12_At3g17000F / W / TTCCGATGATTTCATGAGCCTTCC / 56 / 1200 / Rsa I / 500 / 400 +100
C12_At3g17000R / TCGTGGACCCCTGATTGCAAATTG

Chr: chromosome number, cM: location. Origin: W if primer sequence derives from the website, D if designed in this study. Sequences of the forward (F) and reverse (R) primers (5’-3’), annealing temperature (ToC), fragment length (length), restriction enzyme (Enz.) and band pattern in bp in LA4024 and LA1777 are indicated.

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