Supplementary materials

Table S1.Cultural characteristics of strain YIM 63235T

Agar medium / Colour of mycelium / Soluble pigment
Aerial / Substrate
Czapek’s / white / yellowish brown / absent
Potato-glucose agar / white / yellowish brown / yellowish brown
Nutrient agar / white / orange yellow / absent
Yeast extract-malt extract (ISP 2) / white / yellowish brown / absent
Oatmeal (ISP 3) / white / orange yellow / absent
Inorganic salt-starch (ISP 4) / white / orange yellow / absent
Glycerol asparagine (ISP 5) / white pink / yellowish brown / absent

Table S2. Fatty acid profile (%) of strains YIM 63235Tand P. parietis 04-St-002T

Fatty acid / YIM 63235T / P. parietis 04-St-002T
C14: 0 / 0.14 / 0.71
C15: 0 / 0.87 / 2.23
C16: 0 / 1.29 / 12.03
C17: 0 / 0.83 / 3.84
C18: 0 / - / 1.12
C15: 1ω6c / 0.84 / 0.30
C17: 1ω8c / 1.79 / 3.81
C18: 1ω9c / - / 0.54
iso-C14: 0 / 1.98 / 0.51
iso-C15: 0 / 0.91 / 2.50
iso-C16: 0 / 60.91 / 30.10
iso-C17: 0 / 0.83 / 3.18
iso-C18: 0 / 0.53 / 0.32
iso-C16: 1 H / 22.67 / 5.82
anteiso-C15: 0 / 0.14 / 0.24
anteiso-C17: 0 / 0.98 / 3.87
C16: 010-methyl / 1.09 / 11.82
C17: 010-methyl / 2.24 / 6.84
Summed feature 3 / 1.44 / 9.22

Note: -, negative or absent.

†Summed features are groups of two or three fatty acids that cannot be separated by GLC with the MIDI system. Summed feature 3 contains one or more of the fatty acids C16 : 1ω7c and C15 : 0 iso 2-OH.

For the cellular fatty acid analysis, the two strains YIM 63235T and P. parietis 04-St-002T were obtained by cultivation in shaken flasks (about 200 r.p.m) using tryptic soy broth medium at 28 ºC for 1 week.

Figure S1. Scanning electron micrograph of strain Pseudonocardia antimicrobica YIM 63235T culturedon ISP 2medium for 1 week at 28°C. Bar indicates 5µm.

Fig. S2. Two-dimensional thin-layer chromatogram of polar lipids of YIM 63235T.

Note: 1. diphosphatidylglycerol (DPG); 2. phosphatidylglycerol (PG);

3. phosphatidylcholine (PC); 4. phosphatidylinositol (PI);

5. phosphatidylinositol mannosides (PIM);

6. phosphatidylmethylethanolamine (PME) ; 7. unknown phospholipid (PL).

The chromatographic conditions were as follows: Silica Gel 60 thin-layer plates (10×10cm) were spotted with 10.0 μl of a whole-cell lipid extract. Chloroform-methanol-water (65:25:4, vol/vol/vol) was used in the first direction, and chloroform-acetic acid-methanol-water (80:18:12:5, vol/vol/vol/vol) was used in the second direction. The plate was sprayed with molybdatophosphoric acid reagent.

Fig. S3. Phylogenetic relationships of strain YIM 63235T and other closely related Pseudonocardia species based on 16S rRNA gene sequences. The branching pattern was generated by the neighbour-joining method. Bootstrap values (expressed as percentages of 1000 replications) of above 50% are shown at branch points. Bar, 0.005 substitutions per nucleotide position.

Fig. S4. Maximum-parsimony tree showing the relationships between strain YIM 63235T and members of the genus Pseudonocardia. Kutzneria kofuensis NRRL B-24061T (AF114801) was used as outgroup. Bootstrap percentage based on 1000 resamplings are listed at the nodes.

Fig. S5.Minimum-evolution tree showing the relationships between strain YIM 63235T and members of the genus Pseudonocardia. Kutzneria kofuensis NRRL B-24061T (AF114801) was used as outgroup. Bootstrap percentage based on 1000 resamplings are listed at the nodes.

Fig. S6. Maximum likelihood tree (PHYML) showing the relationships between strain YIM 63235T and members of the genus Pseudonocardia. Kutzneria kofuensis NRRL B-24061T (AF114801) was used as outgroup. Bootstrap percentage based on 1000 resamplings are listed at the nodes.