Table S1. B. Subtilis Strains Used in This Study

Table S1. B. subtilis strains used in this study

Strains / Relevant phenotype / Reference and/or source
JH642 / Prototroph, domesticated strain / (1)
RG4365 / Natto, prototroph, undomesticated strain / (2)
NCIB3610 / Prototroph, undomesticated strain / Bacillus Genetic Stock Center (BGSC)
GP901 / 168 hag::aphA3 / Jörg Stülke, laboratory collection
RG4387 / natto hag::aphA3 / This work, GP901→RG4365
(DNA from donor strain → receptor strain)
TB24 / 3610 hag::aphA3 / This work, GP901→NCIB3610
RG551 / 642 srfAA::cat / B. Lazazzera, laboratory collection
RG4390 / natto srfAA::cat / This work, RG551→RG4365
RG4391 / 3610 srfAA::cat / This work, RG551→NCIB3610
SS995 / Δspo0A::eryr / (3)
RG4370 / natto Δspo0A::eryr / This work, SS995→RG4365
RG4399 / 3610 Δspo0A::eryr / This work, SS995→NCIB3610
RG432 / 642 sinR::pheo / (4)
RG4373 / natto sinR::pheo / This work, RG432→RG4365
RG4392 / 3610 sinR::pheo / This work, RG432→NCIB3610
RG19148 / 642 spoIIAC::kan / (3)
RG4372 / natto spoIIAC::kan / This work, RG19148→RG4365
RG4380 / natto Δspo0A::ery, sinR::pheo / This work, RG4370→RG4373
RG12607 / 642 abrB::spc / (3)
RG12617 / Natto abrB::spc / This work, RG12607→RG4365
RG4381 / natto Δspo0A::ery, abrB::cat / This work, RG4370→RG12607
SIK31 / 642 Δspo0A::ery Pspac-spo0Asad67 / (1)
RG4382 / natto Δspo0A::ery/ Pspac-spo0A-sad67cat / This work, SIK31→RG4365
RG4385 / 3610 hag::aphA3, Δspo0A::ery / This work, RG4399→TB24
AGS207 / 168 tasA::spc / (5)
NRS1502 / 3610 epsG::pBL601 (spc) / (6)
NRS2097 / 3610 bslA::cat / (6)
TB21 / natto blsA::cat / This work, NRS2097→RG4365
TB22 / natto epsG::pBL601 (spc) / This work, NRS1502→RG4365
TB23 / natto tasA::spc / This work, AGS207→RG4365
TB25 / 3610 bslA::cat, hag::aphA3 / This work, NRS2097→TB24
TB26 / 3610 epsG::pBL601 (spc), hag::aphA3 / This work, NRS1502→TB24
TB163 / 3610 tasA::spc / This work, AGS207→NCIB3610
TB228 / 3610 hag::aphA3 tasA::spc / This work, ADS207→TB24
JH12638 / kinA::ery / (7)
RG5000 / natto kinA::ery / This work, JH12638→RG4365
JH19980 / kinB::tet / (7)
RG5001 / natto kinB::tet / This work, JH19980→RG4365
BAL393 / kinC::spc / B. Lazazzera, laboratory collection
RG5002 / natto kinC::spc / This work, BAL393→RG4365
BAL691 / kinD::cat / B. Lazazzera, laboratory collection
RG5003 / natto kinD::cat / This work, BAL691→RG4365
BAL692 / kinE::cat / B. Lazazzera, laboratory collection
RG5004 / natto kinE::cat / This work, BAL692→RG4365
RG5005 / natto kinB::tet kinC::spc / This work, RG5002→RG5001
BAL370 / spo0F::cat / B. Lazazzera, laboratory collection
RG4371 / natto spo0F::cat / This work, BAL370→RG4365
TB40 / 168 amyE::kinB(wt) kan / This work
TB41 / 168 amyE::kinB(ΔK*) kan / This work
TB42 / 168 amyE::kinB(K*→A) kan / This work
TB43 / 168 amyE::kinC (wt)kan / This work
RG5011 / natto kinB::tet amyE::kinB(wt) kan / This work, TB40→RG5001
RG5012 / natto kinB::tet amyE::kinB(K*→A) kan / This work, TB42→RG5001
RG5013 / natto kinB::tet amyE::kinB(ΔK*) kan / This work, TB41→RG5001
RG5021 / natto kinC::spc amyE::kinC (wt)kan / This work, TB43→RG5002
RG5051 / natto kinB::tet kinC::spc amyE::kinB(wt) kan / This work, TB40→RG5005
RG5052 / natto kinB::tet kinC::spc amyE::kinB(K*→A) kan / This work, TB42→RG5005
RG5053 / natto kinB::tet kinC::spc amyE::kinB(ΔK*) kan / This work, TB41→RG5005
RG5006 / natto kinA::ery kinB::tet / This work, RG5000→RG5001
RG5061 / natto kinA::ery kinB::tet amyE::kinB(wt) kan / This work, TB40→RG5001
RG5062 / natto kinA::ery kinB::tet amyE::kinB(K*→A) kan / This work, TB42→RG5001
RG5063 / natto kinA::ery kinB::tet amyE::kinB(ΔK*) kan / This work, TB41→RG5001
RG12604 / natto amyE::abrB-lacZ cat / (2)

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