ESM Table S1 Original identification, localities and accession nos. for cyt b and RAG1 for sequences used in the phylogenetic analyses of Cobitis, Koreocobitis, Microcobitis, Misgurnus, Niwaella, Pangio, Paramisgurnus, and Sabanejewia
Identification / Locality (Country, region, river) / Cyt b mtDNA identification / Cyt b Accession No. / RAG1 ncDNAidentification / RAG1
Accession No. / References
Cobitis biwae (Eastern group, 2n) / JP, Honshu, Mena River, Mutsu River drainage / C. biwae / AB039344 / – / – / Kitagawa et al. (2001)
C. biwae (Western group, 2n) / JP, Honshu, Toki R., Shonai R. d. / C. biwae / AB039345 / – / – / Kitagawa et al. (2001)
C. biwae (Western group, 4n) / JP, Honshu, Ota R. / C. biwae / AB039346 / – / – / Kitagawa et al. (2001)
C. biwae (Kochi group, 2n) / JP, Shikoku, Uranouchi R. / C. biwae / AB039347 / – / – / Kitagawa et al. (2001)
C. biwae / JP, Honshu, Aomori, Iwaki R. / C. biwae / EF508495 / – / – / Šlechtová et al. (2008)
C. biwae (Tetraploid race, 4n) / JP, Honshu, Kyoto, Sonobe R., Ooi R. d. / – / – / C. biwae / AB531331 / Saitoh et al. (2010)
C. biwae (Diploid race, 2n) / JP, Honshu, Hyogo, Kishida R. / – / – / C. biwae / AB531336–37 / Saitoh et al. (2010)
Cobitis choii / KR, Baekgok stream / C. choii / EU656112 / – / – / Kim et al. (2008)
C. choii / KR, Baekgok stream / C. choii / EU670757 / C. choii / EU670833 / Lee (2009)
Cobitis cf. sibirica / RU, Krasnodarskij Kraj, R. Sinukha, Kuban R. d. / C. melanoleuca / AF263068 / C. melanoleuca / EF672428 / Perdices and Doadrio (2001)
Perdices et al. (2008)
Cobitis hankugensis / KR, Namwon-gun, Chollabuk-do / C. hankugensis / AB120176–77 / – / – / Saitoh et al. (2004)
C. hankugensis / KR, Doekcheon R. / C. hankugensis / EU670753 / C. hankugensis / EU670829 / Lee (2009)
Cobitis lutheri / CN, Heihe, Heilongjiang / C. lutheri / DQ105231 / Tang et al. (2006)
C. lutheri / KR, Oa U, Jin Am R. / Not C. lutheri / EF508498–99 / Not C. lutheri / EF508613–14 / Šlechtová et al. (2008)
C. lutheri / KR, Ibpo Stream / Not C. lutheri / EU670754 / – / – / Lee (2009)
Cobitis melanoleuca / RU, Rostov, Don R. / C. melanoleuca / EF508500 / C. melanoleuca / EF056343 / Šlechtová et al. (2008)
Šlecthová et al. (2007)
C. melanoleuca / RU, Rostov, Don R. / – / – / C. melanoleuca / EF508615 / Šlechtová et al. (2008)
Cobitis rara / CN, ornamental fish trade / C. rara / EF508507 / C. rara / EF508619 / Šlechtová et al. (2008)
Cobitis sinensis / Taiwan, South Taiwan, KaoPing / C. sinensis / AM922280 / – / – / Chiang et al. (2010)
C. sinensis / Taiwan, South Taiwan, TzengWen / C. sinensis / AM924181 / – / – / Chiang et al. (2010)
Cobitis striata (4n) / JP, Honshu, Shiga, Hamabun, Lake Biwa / C. striata / AB054125 / C. striata / AB373204–5, 9 / Saitoh et al. (2003),
Saitoh and Chen (2008)
C. striata (Biwa small race, 2n) / JP, Honshu, Shiga, Takashima, Makino, Takagihama, irrigation creek near Lake Biwa / C. striata / AP010782 / C. striata / AB531315–16 / Saitoh et al. (2010)
C. striata (Large race, 4n) / JP, Honshu, Shiga, Hamabun, Lake Biwa / – / – / C. striata / AB373206
AB531290–91 / Saitoh and Chen (2008)
Saitoh et al. (2010)
C. striata (Middle race, 2n) / JP, Honshu, Kyoto, Yagi, Nishida, irrigation creek near Ooi R. / – / – / C. striata / AB531325–26 / Saitoh et al. (2010)
Cobitis shikokuensis (2n) / JP, Shikoku, Ehime, Hiji R. / C. shikokuensis / AP009306 / C. shikokuensis / AB531329–30 / Saitoh et al. (2010)
Cobitis takatsuensis (2n) / JP, Honshu, Misumi R. / C. takatsuensis / AB039337 / – / – / Kitagawa et al. (2001)
C. takatsuensis (2n) / JP, Honshu, Tsutsuga R., Ota R. d. / C. takatsuensis / AB039338 / – / – / Kitagawa et al. (2001)
C. takatsuensis (2n) / JP, Honshu, Shimane, Misumi R. / – / – / C. takatsuensis / EU409616 / Chen et al. (2008)
Iksookimia choii / KR, Yeo Cheong, Mi Ho R. / C. choii / EF508510 / C. choii / EF508622 / Šlechtová et al. (2008)
Koreocobitis naktongensis / KR, Nae Hoa, Geum R. / – / – / K. naktongensis / EF508631 / Šlechtová et al. (2008)
K. naktongensis / KR, Nae Hoa, Geum R. / K. naktongensis / EF508520 / K. naktongensis / EF508632 / Šlechtová et al. (2008)
K. naktongensis / KR, Doekcheon R. / K. naktongensis / EU670764 / K. naktongensis / EU670840 / Lee (2009)
Koreocobitis rotundicaudata / KR, Gyo Hang, Jeon R. / K. rotundicaudata / EF508521
EF508522 / K. rotundicaudata / EF508633
EF508634 / Šlechtová et al. (2008)
K. rotundicaudata / KR, Heuk Stream / – / – / K. rotundicaudata / EU670841 / Lee (2009)
Microcobitis misgurnoides / VN, Thua Thin Hue, Thua Luu R. / M. misgurnoides / EF508503 / M. misgurnoides / EF508616 / Šlechtová et al. (2008)
Misgurnus anguillicaudatus / JP, Hokkaido / M. cf. nikolskyi / AB473261–63 / – / – / Koizumi et al. (2009)
M. anguillicaudatus / JP, Hokkaido / M. anguillicaudatus / AB473264 / – / – / Koizumi et al. (2009)
M. anguillicaudatus / JP, Hokkaido (=Akita, Yamagata and Niigata from Honshu) / M. anguillicaudatus / AB473265 / – / – / Koizumi et al. (2009)
M. anguillicaudatus / JP, Hokkaido (=Aomori and Iwate from Honshu) / M. cf. nikolskyi / AB473277 / – / – / Koizumi et al. (2009)
M. anguillicaudatus / JP, Honshu, Fukushima / M. cf. nikolskyi / AB473308 / – / – / Koizumi et al. (2009)
M. anguillicaudatus / JP, Honshu, Yamaguchi / M. anguillicaudatus / AB473383 / – / – / Koizumi et al. (2009)
M. anguillicaudatus / JP, Shikoku, Tokushima / M. anguillicaudatus / AB473395 / – / – / Koizumi et al. (2009)
M. anguillicaudatus / JP, Kyushu, Fukuoka / M. anguillicaudatus / AB473400 / – / – / Koizumi et al. (2009)
M. anguillicaudatus / JP, Kyushu, Miyazaki / M. anguillicaudatus / AB473407 / – / – / Koizumi et al. (2009)
M. anguillicaudatus / CN, Sichuan, Hejiang / M. anguillicaudatus / AY625700 / – / – / Tang et al. (2005)
M. anguillicaudatus / CN, Shanxi, Luonan / M. mohoity / DQ105238 / – / – / Tang et al. (2006)
M. anguillicaudatus / CN, Hubei, Xianning / M. anguillicaudatus / DQ105240 / – / – / Tang et al. (2006)
M. anguillicaudatus / CN, Guangxi / M. anguillicaudatus / DQ105241 / – / – / Tang et al. (2006)
M. anguillicaudatus / CN, Enshi / M. anguillicaudatus / EF424608 / – / – / Yang et al. Unpub.
M. anguillicaudatus / JP, Honshu, Miyagi, stream in Imaizumi / M. anguillicaudatus / EF508556 / M. anguillicaudatus / EF508651 / Šlechtová et al. (2008)
M. anguillicaudatus / JP, Honshu, Miyagi, stream in Imaizumi / M. cf. nikolskyi / EF508557 / M. anguillicaudatus / EF056344 / Šlechtová et al. (2008)
Šlechová et al. (2007)
M. anguillicaudatus / Taiwan, unknown river / M. anguillicaudatus / EF508559 / M. anguillicaudatus / EF508652 / Šlechtová et al. (2008)
M. anguillicaudatus / CN, Shandong / M. anguillicaudatus / EF595982 / Yang et al. Unpub.
M. anguillicaudatus / CN, Jiangxi, Poyang Lake / M. anguillicaudatus / EU131134 / – / – / Zeng et al. Unpub.
M. anguillicaudatus / CN, Jiangxi, Poyang Lake / M. anguillicaudatus / EU131136 / – / – / Zeng et al. Unpub.
M. anguillicaudatus / CN, Jiangxi, Poyang Lake / M. anguillicaudatus / EU145021–24 / – / – / Zeng et al. Unpub.
M. anguillicaudatus / KR, Okcheon-myeon / M. mohoity / EU670766 / M. anguillicaudatus / EU670842 / Lee (2009)
M. anguillicaudatus / CN, Hubei / M. anguillicaudatus / GU583669 / – / – / Jakovlic et al. Unpub.
Misgurnus bipartitus / CN, Heilongjiang, Yanjie / M. mohoity / DQ105237, 39 / – / – / Tang et al. (2006)
Misgurnus fossilis / CZ, Kosárske' Louky, Dyje R., Danube R. d. / M. fossilis / AF263097 / – / Perdices and Doadrio (2001)
M. fossilis / CZ, D, PL, SK, SLO, UA / M. fossilis / DQ915190 / – / – / Bohlen et al. (2007)
M. fossilis / HR, Poganovo polje / M. fossilis / DQ915197 / – / – / Bohlen et al. (2007)
M. fossilis / UA, Bus'k, channel, Vistula R. d. / M. fossilis / EF508560 / M. fossilis / EF508653 / Šlechtová et al. (2008)
M. fossilis / PL, Warmia-Masuria, Jeglowek Lake / M. fossilis / EF508561 / M. fossilis / EF056339 / Šlechtová et al. (2008) Šlechtová et al. (2007)
M. fossilis / UA, Zhytomyr, Zolochiovka R. / M. fossilis / EF508562 / M. fossilis / EF508656 / Šlechtová et al. (2008)
M. fossilis / UA, Zhytomyr, small stream / M. fossilis / EF508563 / M. fossilis / EF508655 / Šlechtová et al. (2008)
M. fossilis / PL, Warmia-Masuria, Jeglowek Lake / – / – / M. fossilis / EF508654 / Šlechtová et al. (2008)
Misgurnus mizolepis / KR, Biin-myeon / M. dabryanus / EU670767 / M. dabryanus / EU670843 / Lee (2009)
M. cf. mizolepis / CN, Hubei / – / – / M. dabryanus / GU583714 / Jakovlic et al. Unpub.
Misgurnus mohoity / RU, Amur R. / M. mohoity / EF508564 / – / – / Šlechtová et al. (2008)
M. mohoity / RU, Amur R. / M. mohoity / EF508565 / M. mohoity / EF056392 / Šlechtová et al. (2008)
Šlechtová et al. (2007)
Misgurnus nikolskyi / CN, Heilongjiang, Nehe, Nenjiang, Amur R. d. / M. nikolskyi / AB242171 / M. nikolskyi / AB531312 / Saitoh et al. (2006),
Saitoh et al. (2010)
M. nikolskyi / CN, Nenjiang, Nehe, Amur R. b. / – / – / M. nikolskyi / EU711140 / Mayden et al. (2008)
Misgurnus sp. / CN / M. anguillicaudatus / AY281265 / – / – / Wang and Tzeng Unpub.
Misgurnus sp. 1 / RU, Khabarovsk D., Spasovka R. / M. nikolskyi / EF508566 / – / – / Šlechtová et al. (2008)
Misgurnus sp. 1 / RU, Primorye, Karasik R. / M. nikolskyi / EF508567 / M. nikolskyi / EF508658 / Šlechtová et al. (2008)
Misgurnus sp. 2 / KR, Sang Won, Chuk San R. / Misgurnus sp. 2 / EF508569 / Misgurnus sp. 2 / EF508660 / Šlechtová et al. (2008)
Misgurnus sp. 3 / KR, Cheon Gon, Han Tan R. / M. mohoity / EF508568 / M. cf. anguillicaudatus / EF508659 / Šlechtová et al. (2008)
Niwaella delicata / JP, Gifu, Takahara R. / N. delicata / EF508571 / N. delicata / EF508661 / Šlechtová et al. (2008)
N. delicata / JP, Gifu, Obora R. / N. delicata / EF508572 / N. delicata / EF508662 / Šlechtová et al. (2008)
Niwaella multifasciata / KR, Hyeong, Geum R. / N. multifasciata / EF508573 / N. multifasciata / EF508663 / Šlechtová et al. (2008)
N. multifasciata / KR, Hyeong, Geum R. / N. multifasciata / EF508574 / – / – / Šlechtová et al. (2008)
N. multifasciata / KR, Daepyeong-myeon / N. multifasciata / EU670768 / N. multifasciata / EU670844 / Lee (2009)
Pangio oblonga / TH, Chantaburi, unkown river / P. oblonga / EF508581 / P. oblonga / EF508669 / Šlechtová et al. (2008)
Paramisgurnus dabryanus / CN, Anhui, Huangshan / M. dabryanus / AY625701 / Tang et al. (2005)
P. dabryanus / CN, fish market in Wuhan / M. dabryanus / EF508589, 91 / M. dabryanus / EF508676, 75 / Šlechtová et al. (2008)
P. dabryanus / CN, fish market in Shanghai / M. dabryanus / EF508590 / – / – / Šlechtová et al. (2008)
P. dabryanus / Ornamental fish trade / M. dabryanus / EF508592 / – / – / Šlechtová et al. (2008)
Sabanejewia balcanica / RO, Curceni, R. Timis, Danube R. d. / S. balcanica / AF263093 / – / – / Perdices and Doadrio (2001)
S. balcanica / SK, Presov, Ublianka R. / – / – / S. balcanica / EF956336 / Šlechtová et al. (2007)
Sabanejewia romanica / RO, Dragomiresti, R. Dimbovitza, Danube R. d. / S. romanica / AF263095 / – / – / Perdices and Doadrio (2001)
S. romanica / RO, Olt, Oltul R., Danube R. d. / – / – / S. romanica / EF056338 / Šlechtová et al. (2007)
Cyt b and RAG-1 identification refers to phylogenetic position recovered in our analyses
Locality abbreviations: CN China, CZ Czech Republic, HR Croatia, JP Japan, KR (South) Korea, PL Poland, RO Romania, RU Russia, SK Slovakia, SLO Slovenia, TH Thailand, UA Ukraine, VN Vietnams
References:
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