ID / Genotype / Functional category and protein matched name / Theoretical pI/MW(kDa) / Experimental pI/MW(kDa) / MS Score / % Coverage / gi|Number
Photosynthesis-related proteins
2 / Afzal / ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit / 8.92/17.3 / 5.7/14 / 361 / 56 / gi|4038699
3 / Afzal / ribulose bisphosphate carboxylase small chain / 8.98/19.4 / 5.7/10 / 287 / 41 / gi|3914588
4 / Afzal / ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit / 8.92/17.3 / 5.4/13 / 366 / 53 / gi|4038699
8 / Afzal / ribulose bisphosphate carboxylase small chain / 7.62/20.5 / 4.6/24 / 78 / 49 / gi|585791
16 / Afzal / ribulose bisphosphate carboxylase small chain / 5.84/13.3 / 5.3/32 / 188 / 49 / gi|132107
27 / Line527 / ribulose bisphosphate carboxylase small chain / 5.8/13.3 / 5.9/15 / 121 / 30 / gi|132107
30 / Line527 / ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit / 8.9/17.5 / 5.4/13 / 366 / 53 / gi|4038699
31 / Line527 / ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit / 9.1/19.7 / 5.4/14 / 437 / 60 / gi|11990893
39 / Line527 / ribulose bisphosphate carboxylase small chain / 5.8/13.3 / 5.9/30 / 264 / 31 / gi|132107
40 / Line527 / ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit / 8.8/19.7 / 5.9/34 / 378 / 42 / gi|11990897
41 / Line527 / ribulose bisphosphate carboxylase small chain, / 8.9/19.7 / 5.9/28 / 132 / 40 / gi|3914588
47 / Line527 / ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit / 8.8/19.7 / 5.7/33 / 397 / 41 / gi|11990897
48 / Line527 / ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit / 8.8/19.7 / 5.7/31 / 377 / 41 / gi|11990897
50 / Line527 / ribulose bisphosphate carboxylase small chain, / 8.9/19.7 / 6.3/39 / 142 / 26 / gi|3914588
21 / Line527 / ribulose bisphosphate carboxylase/oxygenase activase / 7.59/47.4 / 5.5/43 / 360 / 44 / gi|10720253
49 / Line527 / ribulose 1,5-bisphosphate carboxylase activase isoform 1 [Hordeum vulgare subsp. vulgare] / 8.6/47.3 / 5.6/45 / 202 / 17 / gi|167096
1 / Afzal / oxygen-evolving enhancer protein 2, chloroplast / 8.84/27.3 / 6.34/26 / 312 / 30 / gi|131394
Table 2. Salt responsive proteins of Afzal and Line 527 leaves (resistant and susceptible genotype, respectively) during long-term salt stress with MALDI-TOF-TOF analysis
Table 2. continued
SpotID / Genotype / Functional category and protein matched name / Theoretical pI/MW(kDa) / Experimental pI/MW(kDa) / MS Score / % Coverage / gi|Number
43 / Line527 / oxygen-evolving enhancer protein 2, chloroplas / 8.8/27.4 / 6.3/25 / 259 / 29 / gi|131394
Protein translation, processing, and degradation
7 / Afzal / ribosomal protein P1 [Triticum aestivum] / 4.52/11.2 / 4.4/16 / 80 / 10 / gi|57471718
19 / Afzal / 30S ribosomal protein S1 [Oryza sativa ] / 5.03/23.2 / 4.6/47 / 244 / 33 / gi|149391139
22 / Afzal / ribosomal protein L12 homolog [Oryza sativa] / 5.36/16.3 / 4.9/19 / 324 / 44 / gi|2331135
23 / Afzal / ribosomal protein L12 homolog [Oryza sativa] / 5.36/16.3 / 4.8/19 / 433 / 50 / gi|2331135
35 / Line527 / 30S ribosomal protein S1 [Oryza sativa (indica cultivar-group)] / 5.0/23.2 / 4.6/46 / 244 / 33 / gi|149391139
36 / Line527 / 30S ribosomal protein S1 [Oryza sativa (indica cultivar-group)] / 5.0/23.3 / 4.6/47 / 224 / 26 / gi|149391139
11 / Afzal / cp31AHv protein [Hordeum vulgare subsp. vulgare] / 4.61/31.9 / 4.5/31 / 164 / 25 / gi|3550467
12 / Afzal / cp31BHv [Hordeum vulgare subsp. vulgare] / 4.76/30.6 / 4.5/29 / 410 / 37 / gi|3550483
34 / Line527 / cp31AHv protein [Hordeum vulgare subsp. vulgare] / 4.6/31.9 / 4.4/29 / 475 / 31 / gi|3550467
32 / Line527 / chloroplast-localized cyclophilin [Triticum aestivum] / 8.5/16.5 / 6.8/18 / 362 / 40 / gi|83700338
20 / Afzal / subtilisin-chymotrypsin inhibitor CI-1B, putative, expressed [Oryza sativa (japonica cultivar-group)] / 5.24/8.1 / 4.9/42 / 73 / 42 / gi|77556084
Signal transduction
13 / Afzal / translationally-controlled tumor protein homolog / 4.53/18.9 / 4.7/25 / 120 / 27 / gi|20140865
Cell wall-related proteins
18 / Afzal / germin-like protein 1 [Oryza sativa] / 6.01/22.0 / 5.6/22 / 94 / 27 / gi|4239821
28 / Line527 / profilin-1 / 5.0/14.4 / 4.8/10 / 167 / 24 / gi|1709779
42 / Line527 / oxalate oxidase-like protein or germin-like protein [Hordeum vulgare subsp. vulgare] / 6.0/24.8 / 6.5/26 / 243 / 37 / gi|1070358
Table 2. continued
SpotID / Genotype / Functional category and protein matched name / Theoretical pI/MW(kDa) / Experimental pI/MW(kDa) / MS Score / % Coverage / gi|Number
Energy metabolism
15 / Afzal / nucleoside diphosphate kinase [Lolium perenne] / 6.30/16.5 / 6.5/14 / 256 / 35 / gi|9652119
Transcription factor
10 / Afzal / NACA3 (Nascent polypeptide associated complex subunit alpha-like 3) [Arabidopsis thaliana] / 4.96/17.9 / 5.1/17.9 / 102 / 28 / gi|15240731
33 / Line527 / NACA3 (Nascent polypeptide associated complex subunit alpha-like 3) [Arabidopsis thaliana] / 4.96/17.9 / 5.1/17.9 / 102 / 28 / gi|15240731
ROS scavenging and defense
5 / Afzal / putative glycine decarboxylase subunit [Triticum aestivum] / 4.99/21.2 / 4.5/14 / 167 / 28 / gi|22204118
6 / Afzal / putative glycine decarboxylase subunit [Triticum aestivum] / 4.99/21.2 / 4.5/13 / 257 / 28 / gi|22204118
37 / Line527 / Os06g0232600 [Oryza sativa (japonica cultivar-group)] / 7.7/29.8 / 5.1/27 / 80 / 22 / gi|115467258
25 / Afzal / Os04g0530600 [Oryza sativa (japonica cultivar-group)] / 9.16/19.4 / 5.01/11 / 155 / 19 / gi|115459582
29 / Line527 / chain A, crystal structure of barley thioredoxin H isoform 2 / 5.1/13.3 / 5.0/9 / 83 / 16 / gi|186972808
38 / Line527 / putative peptide methionine sulfoxide reductase [Oryza sativa] / 6.2/23.1 / 5.7/25 / 85 / 15 / gi|12597894
Unclassified proteins
17 / Afzal / polyamine oxidase [Hordeum vulgare subsp. vulgare] / 6.19/65.5 / 5.1/27 / 81 / 26 / gi|14485487
26 / Line527 / USP family protein [Triticum aestivum] / 5.8/13.3 / 5.9/15 / 121 / 30 / gi|60100214
Spots identified by MALDI-TOF-TOF MS analysis from 2-DE gels are reported. Spot number, genotype, protein name, theoretical and experimental pI and Mr values, MS Score, % coverage and gi|Number are listed. Increasing/decreasing index (fold change) was calculated as the ratio of spot intensities (relative volumes) for salt treatment with respect to control. Proteins were considered as differentially expressed when a relative fold change > 1.5 or < 0.5 was measured.