Supplementary 1

Table 1: Primers and restriction enzymes as described by Milner and Campbell; [1992].

S.No / Polymorphism / Primers / Amplicon Length / Restriction Enzyme and fragment lengths
1 / HSP-70-1 +190G/C
(rs1043618) / F: CGCCATGGAGACCAACACCC
R: GCGGTTCCCTGCTCTCTGTC / 488bp / BsrBI: 461+27
2 / HSP-70-2 +1267A/G
(rs1061581) / F: CATCGACTTCTACACGTCCA
R: CAAAGTCCTTGAGTCCCAAC / 1118bp / Pst1: 934+184
3 / HSP-70-hom +2437T/C
(rs2227956) / F: GTCCCTGGGGCTGGAGACGG
R: GATGATAGGGTTACACATCTGCT / 627bp / NcoI: 354+273

Table 2: Ion channel gene defects in correlation to HSP-70 risk alleles

SNP / Risk Allele / Cases / KCNQ1 / KCNE1 / SCN5A
IVS13+36A/G / S546S / S38G / C3417A (S1074A)
AA / AG / GG / AA / AG / GG / AA / AG / GG / CC / CA
rs1043618
HSP-70-1 / C / 34 / 13 / 11 / 10 / 13 / 11 / 10 / 5 / 16 / 13 / 21 / 13
rs1061581
HSP-70-2 / G / 39 / 14 / 10 / 15 / 14 / 10 / 15 / 5 / 17 / 17 / 23 / 16
rs2227956
HSP-70-hom / C / 41 / 14 / 11 / 16 / 14 / 12 / 15 / 5 / 19 / 17 / 26 / 15
SNP / Risk Allele / FDRs / KCNQ1 / KCNE1 / SCN5A
IVS13+36A/G / S546S / S38G / C3417A (S1074A)
AA / AG / GG / AA / AG / GG / AA / AG / GG / CC / CA
rs1043618
HSP-70-1 / C / 49 / 9 / 20 / 20 / 9 / 20 / 20 / 5 / 27 / 17 / 17 / 32
rs1061581
HSP-70-2 / G / 56 / 9 / 19 / 28 / 9 / 19 / 28 / 4 / 33 / 19 / 18 / 38
rs2227956
HSP-70-hom / C / 52 / 9 / 15 / 28 / 9 / 15 / 28 / 6 / 29 / 17 / 15 / 37

*The table represents a part of the mutation analysis data from the ongoing study.

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