Table 1 PCR Primers Used to Amplify CSB Cdna

Forward (5’– 3’) / Reverse (5’– 3’) / Product size (bp)
CGG TAG CGT CTC TGT TTC CT / ACC TCT GCT CCT CCA AGG AT / 668
CGT TGA CGT GGA GAA GGA GT / AAC TGC AAA GCC CTC TTC TG / 648
TGG CAG ATC AAG CAA AAC TG / AAT CGT CCT CCA GCT TCA GA / 633
GAA ACG GCA GAA GAA AGT GC / CCA TAA ACA CAG GCA ACG TG / 881
CAT TCG ATT GAT GCA GGA TG / TTT TCC CAG AAC GTT TCC AG / 641
TGA GCC TTT CTT TGC CAG AT / ATC ATG GTC TGC TCC AAA GG / 903
GGC CAG AAG AAG CAA GTG AC / AGG CTT TTG CTT TGG TCT CA / 765
GGA AAT GGG GAA TGT TCA AA / TCC CTC CTT TTT CAT GAT GC / 714
AAA GCA CTG AGG CTC TCT CG / GCC CGT TAG AAA AAG GGA CT / 644

Table 1 PCR primers used to amplify CSB cDNA.

CSB
exons / Forward (5’– 3’) / Reverse (5’– 3’) / Product size (bp)
1 / CCC AAG CTG ACT TTC TCT GC / GCT ACT TCC GGT CTC AGG TG / 642
2
(part 1) / GTG GCT GGC TTT CTT AGC TG / TAG ACG TCC ACA CCA AAC CC / 580
2
(part 2) / CTT TCG TTC TGT GGG TGA CG / GCT GGG ACT CAA AGA TGA GG / 452
3 / TGA AGC AGC TTA CAA ATG GG / TTT GTC GGG ATG TAA CCC TG / 649
4 / GGT AGT TGC TCA CAT AGG GC / TGA GAG GGA TTT GGT TCT GC / 462
5
(part 1) / AGC CTA TCT TTG GAT GGC AG / CTT CAC CCT CAG AGT CTC CC / 734
5
(part 2) / ACC AAA CAA GAA AGC CAG AG / GGT TAA GGC TGC AGA AAT CC / 602
6 / ATG AAT TGA GAT GGC TGG AG / TGC TTT TAG TCT GCT GAG GC / 579
7 / ATC CCG CAT GTT TCT CTG AC / CTG CCT ACA GCT CCA TTG TC / 525
8 / TGT GGC TTG AAA ACT CTT GG / GCC CTG TCT GAG ATG ATT CG / 827
9 / CTC CTT CCC CTA ATA ACG GC / TAA CAA CTG GCC CCT ACA AG / 605
10 / TTG TAG GGG CCA GTT GTT AG / CCC AGG AAC AAT GCC AAA AC / 727
11 / CCA TTT TGC AAT CAT TTC CC / GAA GAA TCC ACT GAG GGC AG / 421
12 / TGG TGA GCA GGG TAT GGT GA / CAT CTA CCA TGC GGG ACT TC / 417
13 / CAG CAA TTG AAT ATG ACC CC / GAA ATC GGT AGA CTG CCA CC / 612
14 / CCT GAG CTT TGG CTG TTT AC / CAA CAA GTC TCC CCT TAG GC / 457
15 / GCC TAA GGG GAG ACT TGT TG / GCT GAA GAT GGT TCC TGT CC / 635
16 / GGA CAG GAA CCA TCT TCA GC / CAA CAC ACC ATA GCA GGC AG / 673
17 / TCT GCA AGA CCT GGG AGA TT / ATC ATG GTC TGC TCC AAA GG / 501
18
(part 1) / GAC TAG TCC TGA TGC ATC CC / TTG AGC CTG GCT GGG TCT TT / 557
18
(part 2) / TGC AGT ATC TGG ACC AGA AG / CCA TGA GTG GCC ATA AGT AC / 681
19 / GGC TTT ACA CAT TGC TTG GG / TGG AAA ACA GGG GAT GAA AG / 722
20 / AAA TGG CAT GGC TGT GAA AG / GCG TGC AAC ACA AAT ATC AG / 347
21 / TCA GTC TCA CCC TGT CAA CC / TGC AAA CAA ACA TCA AGT GC / 669

Table 2 PCR primers used to amplify CSB exons.

Forward (5’– 3’) / Reverse (5’– 3’) / Product size (bp)
TGG TGA TTG AGC CTG TCA GC / GGT CTC ATT TGG AGG GTT GG / 647
TAG GTC ATG CCA TCA TCA GC / AGG TAG ATG TGG GGT CAA GG / 761
TTC ACA CGT TGT TTC CTT GC / GGA GGA TCA CGA GGT CAG AA / 600
CCT AGT ACA TTG CCT GGT GT / AAC ACT GAT GAT TGC TGT GG / 774
GAG ATT GTT CAG CCC TGG AG / GTT TCA GTG GAG CGA TAG GG / 615
TGT CTC CCT CCA GCC ATT AC / AAA AGG ATG CCA ATT CAA CC / 742
TCA TTC ACT GGA CGC TCT TC / TCT GCT GAA AGG AAG TGA GG / 676
GCA TGC TGC AAA TGG TAC TG / CAA ATC AGG ATG GGG AAG AG / 699
TCT CCT TAA AGC AGC AGG TC / CAT TCC AGC ATA GGC AAC AG / 572
GCT TGA CAA AGG TCA TGT GC / CCA GCC TGG GAA AGA TAA TG / 757
TCA GCC CCT TGA GTA ACT GG / TTC CCT CAT TTG GCA TTC TC / 757

Table 3 PCR primers used to amplify CSB intron 1.