Table 1: List of Up- Or Down-Regulated Genes in Smuvick Microarray Relative to UA159

SUPPLEMENTARY MATERIAL

Tables and Figures

Table 1: List of up- or down-regulated genes in SmuvicK microarray relative to UA159

Table S2: Primers used in this study

Figure S1: Schematic overview of selected genes obtained by global transcriptional analysis that were shown as significantly affected (p<0.001) upon VicK mutagenesis relative to UA159. Cells were grown in THYE until mid-logarithmic phase, used for RNA extraction and cDNA synthesis. ↑ denotes upregulated genes, whereas ↓ denotes downregulated genes. Gene IDs shown in regular font represent genes that were either up- or down-regulated in the microarray, whereas those in bold font represent genes with putative functions in bacteriocin production, cell wall metabolism and bacteriocin immunity that were successfully confirmed as differentially expressed by qRTPCR in this study. Under the cell wall metabolism category, the underlined genes did not appear as differentially expressed in our SmuvicK microarray. Gene identities represent those from the National Institute of Health (NIH) genetic sequence database (Genbank). However, we successfully confirmed these candidates as VicK-modulated using qRTPCR in this study (data not shown).

Figure S2: Relative expression of bacteriocin immunity genes SMU.1913 (immA), SMU.925 (immB) and SMU.1914 (nlmC) contiguously located with immA. Cultures were grown in THYE until mid-log phase and cDNAs were extracted from S. mutans SmuvicK (dark grey) and Smuvic+ (light grey) strains. Expression in the S. mutans UA159 parent was set at a user-defined value of 1.0. Results were normalized with 16SrRNA expression. Results show mean expression ± std. error from triplicate experiments.