Table 1: Differentially Expressed Proteins in BM and PB Plasma Derived from Patients At

The identified proteins are designated with their abbreviated names and the Swiss-Prot accession numbers. The theoretical molecular weight (MW) calculated by CalPI/MW; available in the Swiss-Prot Web site, the theoretical isoelectric point (pI), the probability of a random identification (Mascot score), the number of the peptides used for identification and coverage of each protein are also demonstrated. Score is 10*log (p), where P the probability of the observed match to be a random event. Mascot scores >55 indicate identity, or extensive homology at the p<0.05 level. Expression levels >1 suggest overexpression, whilst those <1 indicate suppression (*p, 0.05, **p, 0.005).Where: BMP; BM plasma and PBP; PB plasma. •AML; consider both MDS/AML and de novo AML

The identified proteins are designated with their abbreviated names and the Swiss-Prot accession numbers. The theoretical molecular weight (MW) calculated by CalPI/MW; available in the Swiss-Prot Web site, the theoretical isoelectric point (pI), the probability of a random identification (Mascot score), the number of the peptides used for identification and coverage of each protein are also demonstrated. Score is 10*log (p), where P the probability of the observed match to be a random event. Mascot scores >55 indicate identity, or extensive homology at the p<0.05 level. Expression levels >1 suggest overexpression, whilst those <1 indicate suppression (*p, 0.05, **p, 0.005).Where: BMC; BM cells and PBC; PB cells. •AML; consider both MDS/AML and de novo AML

The identified proteins are designated with their abbreviated names and the Swiss-Prot accession numbers. The theoretical molecular weight (MW) calculated by CalPI/MW; available in the Swiss-Prot Web site, the theoretical isoelectric point (pI), the probability of a random identification (Mascot score), the number of the peptides used for identification and coverage of each protein are also demonstrated. Score is 10*log (p), where P the probability of the observed match to be a random event. Mascot scores >55 indicate identity, or extensive homology at the p<0.05 level. Expression levels >1 suggest overexpression, whilst those <1 indicate suppression (*p, 0.05, **p, 0.005).Where: PF-BMP; pre-fractionated BM plasma and PF-PBP; pre-fractionated PB plasma. •AML; consider both MDS/AML and de novo AML.

Netwotrk Protein Analysis. Differentially expressed proteins, identified in the present study, were used for pathway analysis. For this purpose, the Swiss-Prot accession numbers were inserted into the String software available at * REFERENCE: Jensen LJ, Kuhn M, Stark M, Chaffron S, Creevey C, Muller J, et al.. STRING 8--a global view on proteins and their functional interactions in 630 organisms. Nucleic Acids Res. 2009, 37(Database issue): D412-416.

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Figure 4: Expression Profiling Diagrams of the BM & PB plasma overexpressed proteins in A. MDS and B. AML, respectively. The diagrams were constructed with the use of the String software.

Figure 5: Western blot analysis of MOES (68kDa) expression in de novo AML patients (Lanes 1 and 2), MDS/AML patients (Lanes 3 and 4) and non leukemic patients (Lanes 5-8).