ANNEX 1
Statistical analysis
Table 1. Anova from the Remazol Blackpreselection experiment
Model / degrees of Freedom / Sum of squares / Mean square / F / p-valueModel / 16 / 1.65334552 / 0.10333409 / 8.56 / <.0001
Error / 28 / 0.33793603 / 0.01206914
Model / degrees of Freedom / Sum of squares / Mean square / F / p-value
Block / 2 / 0.02180169 / 0.01090084 / 0.90 / 0.4168
Treatment / 14 / 0.91873077 / 0.06562363 / 12.12 / <.0001
Table 2. Duncan’s test Remazol Black preselection.
Fungal isolate / Mean / Duncan’s testLVN 6 / 0.89751 / A
LVN 88 / 0.83327 / A
LVN 63 / 0.82324 / A
LVN 61 / 0.65534 / B
LVN 80 / 0.62343 / C / B
LVN 49 / 0.57973 / C / B / D
LVN 44 / 0.55713 / C / B / D
LVN 71 / 0.55005 / C / B / D
LVN 72 / 0.53513 / C / B / D
LVN 54 / 0.51505 / C / B / D
LVN 48 / 0.46802 / C / E / D
LVN 52 / 0.43404 / D / E
LVN 74 / 0.32250 / E / F
LVN 45 / 0.31218 / E / F
LVN 35 / 0.20206 / G
Table 3Anova from the Turquoise Blue preselection experiment
Model / degrees of Freedom / Sum of squares / Mean square / F / p-valueModel / 16 / 1.6534 / 0.103388 / 2.68 / 0.01
Error / 28 / 1.08136889 / 0.03862032
Model / degrees of Freedom / Sum of squares / Mean square / F / p-value
Blocks / 2 / 0.79645778 / 0.05688984 / 1.47 / 0.1857
Isolates / 14 / 0.85696444 / 0.42848222 / 11.09 / 0.0003
Table 4. Duncan’s test Turquoise Blue preselection test
Fungal isolate / Mean / Duncan’s testLVN 6 / 80.00 / A
LVN 49 / 75.67 / B / A
LVN 63 / 72.67 / B / A / C
LVN 80 / 68.33 / B / A / C
LVN 72 / 67.00 / B / A / C
LVN 61 / 63.67 / B / C
LVN 71 / 59.33 / B / C
LVN 44 / 59.00 / B / C
LVN 45 / 58.33 / B / C
LVN 54 / 55.67 / B / C
LVN 74 / 54.00 / B / C
LVN 35 / 51.67 / B / C
LVN48 / 47.33 / B / C
LVN88 / 43.33 / C
LVN52 / 41.67 / C
Table 5. Anova from the Novacron Red preselection experiment.
Model / degrees of Freedom / Sum of squares / Mean square / F / p-valueModel / 16 / 0.92010088 / 0.05750630 / 10.62 / <.0001
Error / 28 / 0.15157902 / 0.00541354
Model / degrees of Freedom / Sum of squares / Mean square / F / p-value
Blocks / 2 / 0.00137010 / 0.00068505 / 0.13 / 0.8816
Isolates / 14 / 0.91873077 / 0.06562363 / 12.12 / <.0001
Table 6. Duncan’s test Novacron Redpreselection experiment
Fungal isolate / Mean / Duncan’s testLVN 80 / 0.86084 / A
LVN 61 / 0.85062 / A
LVN 6 / 0.81965 / B / A
LVN 63 / 0.81049 / B / A
LVN 88 / 0.78686 / B / A / C
LVN 49 / 0.73266 / B / D / C
LVN 54 / 0.73239 / B / D / C
LVN 45 / 0.67851 / D / C
LVN 48 / 0.65252 / E / D
LVN 72 / 0.64699 / E / D
LVN 44 / 0.64295 / E / D
LVN 71 / 0.54642 / E / F
LVN 35 / 0.52072 / F
LVN 52 / 0.45356 / G / F
LVN 74 / 0.37372 / G
Table7. Anova from the Remazol Black selection experiment.
Model / degrees of Freedom / Sum of squares / Mean square / F / p-valueModel / 11 / 3.91936389 / 0.35630581 / 23.39 / <.0001
Error / 24 / 0.36560000 / 0.01523333
Total / 35 / 4.28496389
Model / degrees of Freedom / Sum of squares / Mean square / F / p-value
Isolates / 3 / 3.26020833 / 1.08673611 / 71.34 / <.0001
Ammonium tartrate concentration / 2 / 0.32117222 / 0.16058611 / 10.54 / 0.0005
Isolates *
Ammonium tartrate concentration / 6 / 0.33798333 / 0.05633056 / 3.70 / 0.0096
Figure 1. Interaction plot Remazol Black a) Isolates. b) ammonium tartrate concentration.
Table8.Anova from the Turquoise Blue selection experiment
Model / degrees of Freedom / Sum of squares / Mean square / F / p-valueModel / 17 / 2.78741481 / 0.16396558 / 10.71 / <.0001
Error / 36 / 0.55133333 / 0.01531481
Total / 53 / 3.33874815
Model / degrees of Freedom / Sum of squares / Mean square / F / p-value
Isolates / 5 / 0.99345926 / 0.19869185 / 12.97 / <.0001
Ammonium tartrate concentration / 2 / 0.60600370 / 0.30300185 / 19.78 / <.0001
Isolates *
Ammonium tartrate concentration / 10 / 1.18795185 / 0.11879519 / 7.76 / <.0001
Figure 2. Interaction plot Turquoise Bluedecolorization% a) Isolates. b) Ammonium tartrate concentration.
Table 9. Anova from the Novacron Red selection experiment
Model / degrees of Freedom / Sum of squares / Mean square / F / p-valueModel / 17 / 3.08693333 / 0.18158431 / 36.92 / <.0001
Error / 36 / 0.17706667 / 0.00491852
Total / 53 / 3.26400000
Model / degrees of Freedom / Sum of squares / Mean square / F / p-value
Isolates / 5 / 1.66071111 / 0.33214222 / 67.53 / <.0001
Ammonium tartrate concentration / 2 / 1.09954444 / 0.54977222 / 111.78 / <.0001
Isolates *
Ammonium tartrate concentration / 10 / 0.32667778 / 0.03266778 / 6.64 / <.0001
Figure 3. Interaction plot Novacron Red decolorization %: a) Isolates. b) ammonium tartrate concentration.
Table 10. Anova from the commercial laccase experiment using Remazol Black
Model / Degrees of Freedom / Sum of squares / Mean square / F / p-valueModel / 5 / 1.98129835 / 0.39625967 / 155.10 / <.0001
Error / 12 / 0.03065854 / 0.00255488
Total / 17 / 2.01195689
Model / Degrees of Freedom / Sum of squares / Mean square / F / p-value
ABTS concentration / 2 / 1.84223208 / 0.92111604 / 360.53 / <.0001
Laccase concentration / 1 / 0.08992988 / 0.08992988 / 35.20 / <.0001
ABTS concentration*
Laccase concentration / 2 / 0.04913639 / 0.02456819 / 9.62 / 0.0032
Figure 4. Interaction plot Remazol Black decolorization %. a) Laccase concentration b) ABTS concentration.
Table 11. Anova from the commercial laccase experiment using Novacron Red
Model / Degrees of Freedom / Sum of squares / Mean square / F / p-valueModel / 5 / 1.32639969 / 0.26527994 / 52.14 / <.0001
Error / 12 / 0.06105089 / 0.00508757
Total / 17 / 1.38745057
Model / degrees of Freedom / Sum of squares / Mean square / F / p-value
ABTS concentration / 2 / 1.32128226 / 0.66064113 / 129.85 / <.0001
Laccase concentration / 1 / 0.00004235 / 0.00004235 / 0.01 / 0.9288
ABTS concentration*
Laccase concentration / 2 / 0.00507508 / 0.00253754 / 0.50 / 0.6193
Table 12. Novacron Red commercial laccase experimentDuncan’s test
Fungal isolate / Mean / Duncan’s test1mM - 1mg/mL / 0.60685 / A
2mM - 2mg/mL / 0.60685 / A
1mM - 2mg/mL / 0.56839 / A
2mM - 1mg/mL / 0.54317 / A
0mM - 2mg/mL / 0.00388 / B
0mM - 1mg/mL / 0.00000 / B
Table 13. Anova from the commercial laccase experiment using Turquoise blue
Model / Degrees of Freedom / Sum of squares / Mean square / F / p-valueModel / 5 / 1.28314305 / 0.25662861 / 45.65 / <.0001
Error / 12 / 0.06746260 / 0.00562188
Total / 17 / 1.35060565
Model / Degrees of Freedom / Sum of squares / Mean square / F / p-value
ABTS Concentration / 2 / 0.69393678 / 0.34696839 / 61.72 / <.0001
Laccase concentration / 1 / 0.47512880 / 0.47512880 / 84.51 / <.0001
ABTS concentration*
Laccase concentration / 2 / 0.11407747 / 0.05703873 / 10.15 / 0.0026
Figure 5. Interaction plot Remazol Black decoloration %. a) Laccase concentration b) ABTS concentration.
Table14.Anova from the final effluent experiment
Model / Degrees of Freedom / Sum of squares / Mean square / F / p-valueModel / 7 / 2.53236676 / 0.36176668 / 34.76 / <.0001
Error / 16 / 0.16652800 / 0.01040800
Total / 23 / 2.69889476
Model / Degrees of Freedom / Sum of squares / Mean square / F / p-value
pH / 1 / 2.36364584 / 2.36364584 / 227.10 / <.0001
supplementation / 1 / 0.01585154 / 0.01585154 / 1.52 / 0.2350
pH* supplementation / 1 / 0.04365657 / 0.04365657 / 4.19 / 0.0573
inoculum / 1 / 0.05864361 / 0.05864361 / 5.63 / 0.0305
pH*inoculum / 1 / 0.00207334 / 0.00207334 / 0.20 / 0.6613
inoculum*supplementation / 1 / 0.03357129 / 0.03357129 / 3.23 / 0.0914
pH*inoculum*supplementation / 1 / 0.01492458 / 0.01492458 / 1.43 / 0.2486
Table15.Final effluent experimentDuncan’s test
Treatment / Mean / Duncan’s testpHf-Sup-8 / 95.553 / A
pHf-Sup-4 / 71.340 / B
pHf-No-4 / 70.138 / B
pHf-No-8 / 69.416 / B
pHi-No-8 / 18.309 / C
pHi-Sup-8 / 17.412 / C
pHi-No-4 / 12.775 / C
pHi-Sup-4 / 5.414 / C
Table 16. Anova from the dyeing effluent experiment
Model / degrees of Freedom / Sum of squares / Mean square / F / p-valueModel / 7 / 2.23674328 / 0.31953475 / 23.64 / <.0001
Error / 15 / 0.20277194 / 0.01351813
Total / 22 / 2.43951523
Model / degrees of Freedom / Sum of squares / Mean square / F / p-value
Dilution / 1 / 1.61315972 / 1.61315972 / 119.33 / <.0001
Supplementation / 1 / 0.20239134 / 0.20239134 / 14.97 / 0.0015
Dilution*supplementation / 1 / 0.12121427 / 0.12121427 / 8.97 / 0.0091
inoculum / 1 / 0.06939702 / 0.06939702 / 5.13 / 0.0387
Dilution*inoculum / 1 / 0.01964423 / 0.01964423 / 1.45 / 0.2467
inoculum*Supplementation / 1 / 0.07811871 / 0.07811871 / 5.78 / 0.0296
Dilution*inoculum*Supplementation / 1 / 0.13281799 / 0.13281799 / 9.83 / 0.0068
Figure 6.Dyeing effluentinteraction plot decoloration% dyeing effluent a) with and whitout supplementation b) inoculum