Synthesis and characterization of superparamagnetic nanoparticles coated with fluorescent gold nanoclusters- Supporting information
Xavier Le Guévela, Eva-Marie Prinzb, Robert Müllerc, Rolf Hempelmannb, Marc Schneidera
aPharmaceutical Nanotechnology, Saarland University, Saarbrücken, Germany
bDepartment of Physical Chemistry, Saarland University, Saarbrücken, Germany
cInstitute for Photonic Technology, P.O box 100239, Jena, Germany
Figure S1. X-ray diffraction (XRD) of the typical reflexes of Mn0,8Zn0,2Fe2O4 called mNPs compared to the JCPDS database.
Figure S2. FTIR spectra (left) and zeta potential (right) of the different polyelectrolyte layers PAH, PAA on mNPs. Datashowedthe adsorption of the polyeletrolytes on the magnetic nanoparticles with the characteristic vibration bands and the change of the surface charge after each monolayer.
Wave number [cm-1] / group / Vibration type / Literature[1] [cm-1]3625-3125 / O-H / Stretching vibration of the carboxyl and / 3300-2500
3450-3300 / H2O / The two stretching vibrations of the water molecule / 3450-3300
3625-3125 / N-H / Stretching vibration of the amino group and / 4000-3200
3000-2750 / C-H / Stretching vibration of aliphatic -CH2 groups peak splits to a doublet / 3100-2800
1500-1250 / C-H / Deformation vibration ofaliphatic -CH2 groups / 1470-1350
1725 / C=O / Stretching vibration of the carboxyl group / 1725-1700
1545 / COO- / Asymmetric stretching vibration of deprotonated carboxylate group / 1610-1300
1600 / N-H / Deformation vibration of primary amides / 1650-1580
1440 / C-O/O-H / Combination band due to C-O stretching and O-H deformation vibration / 1440-1395
1400 / -CH2CO- / Deformation vibration of
-CH2CO- / 1410-1405
1110 / C-C / Stretching vibration / 1230-1160
1000 / C-N / Stretching vibration / 1090-1020
920 / C-H / Deformation vibration / 975-780
Table S1: List of the different vibration peaks of the coated particles*.
*G. Socrates, Infrared Characteristic Groups Frequencies "Tables and Charts", 2. Auflage, Wiley, 1994.
Figure S3. MALDI-TOF MS measurement of AuBSA using - Cyano-4-hydroxycinnamic acid (CHCA) as matrix. The spectrum was collected in the positive mode and shows a Gaussian distribution of peaks separated by m/z 197 and 32 (expended view) corresponding to gold and sulphur.
Figure S4. XPS spectra of Au 4f were fitted (purple and yellow lines) and confirmed the presence of two distinct doublet Au 4f7/2 peaks at 84 eV andthe other at 85.4 eV, assigned to Au (0) (88%) and Au (I) (12%) respectively.
Figure S5. Surface charge determined by zeta potential (mV) of AuBSA as a function of the pH.
Figure S6. EDX measurement of AuBSA performed on the protein shell of mNPs-PE-AuBSA.
Figure S7. Infrared spectra of mNPs-PE-AuBSA after each step of the synthesis between 800 and 3800 cm-1. Samples were freeze-dried before measurements.
Figure S8. Excitation spectra of diluted AuBSA solutions with fluorescence emission at = 670 nm (Gain= 100).
Figure S9. Excitation/emission spectra (left) and absorbance spectrum (right) of AuBSA solution.
Figure S10. Magnetic measurement of AuBSA indicating the diamagnetic property of the gold nanoclusters in BSA.