SupplementalData:

Supplemental Table S1: Bioinformation analysis of sites subjected to proFaSnRK2.6 DNA methylation. (

Methylated site / Sequence / Prediction of promoter element
Species Function / Article
-2050 / NO / NO / NO / NO
-1912 / SCGAYNRNNNNNNNNNNNNNNNHD / Chlamydomonas / Consensus sequence of PRE (plastid response element) in thepromoters of HSP70A in Chlamydomonas; Involved in induction of HSP70A gene by both MgProto and light; / Gromoff ED, Schroda M, Oster U, Beck CF (2006) Identification of a plastid response element that acts as an enhancer within the Chlamydomonas HSP70A promoter. Nucleic acids research34: 4767-4779
-1852/-1846/ -1839 / CTGACY / Nicotiana tabacum / "W box" identified in the region between -125 and -69 of a tobacco class I basic chitinase gene CHN48; NtWRKY1, NtWRKY2 and NtWRKY4 bound to W box; NtWRKYs possibly involved in elicitor-respsonsive transcription of defense genes in tobacco; / Yamamoto S, Nakano T, Suzuki K, Shinshi H (2004) Elicitor-induced activation of transcription via W box-related cis-acting elements from a basic chitinase gene by WRKY transcription factors in tobacco. Biochimica et BiophysicaActa (BBA)-Gene Structure and Expression1679: 279-287
-1754 / NGATT / Arabidopsis thaliana / "ARR1-binding element" found in Arabidopsis; ARR1 is a response regulator; AGATT is found in the promoter of ricenon-symbiotic haemoglobin-2 (NSHB) gene; / Sakai H, Aoyama T, Oka A (2000)Arabidopsis ARR1 and ARR2 response regulators operate as transcriptional activators. The Plant Journal24: 703-711
Ross EJ, Stone JM, Elowsky CG, Arredondo-Peter R, Klucas RV,Sarath G (2004) Activation of the Oryza sativa non-symbiotic haemoglobin-2 promoter by the cytokinin-regulated transcription factor ARR1. Journal of experimental botany55: 1721-1731
-1140/-1136 / VCGCGB / Arabidopsis thaliana / "CGCG box" recognized by AtSR1-6 (Arabidopsis thaliana signal-responsive genes); Multiple CGCG elements are found in promoters of many genes; Ca++/calmodulin binds to all AtSRs; / Yang T, Poovaiah BW (2002) A calmodulin-binding/CGCG box DNA-binding protein family involved in multiple signaling pathways in plants. Journal of Biological Chemistry277:45049-45058
-1117/-1109 / RYCGAC / Hordeumvulgare / Binding site of barley (H.v.) CBF1 and also of barley CBF2; CBF=C-repeat (CRT) binding factors; CBFs are also known as dehydration-responsive element (DRE) binding proteins (DREBs); / Xue GP (2002) Characterisation of the DNA‐binding profile of barley HvCBF1 using an enzymatic method for rapid quantitative and high‐throughput analysis of the DNA‐binding activity. Nucleic acids research30: e77-e77

Y=C/T;H=T/C/A; D=A/T/G;N=G/A/C/T;V=A/C/G; B=G/T/C;R=A/G; S=G/C;

Supplemental Table S2: Primers used to amplify FaSnRK2.1 to FaSnRK2.9 coding sequences and GenBank accession numbers.

Gene / Forward primers (5’-3’) / Reverse primers (5’-3’) / Genbank accession numbers
FaSnRK2.1 / ATGGAGAGGTATGAGATAGTGAAAG / TCACAACGCACATACAAAATCACC / KJ748362
FaSnRK2.2 / ATGGAGAAGTATGAGGTTGTGAAAG / TCATTCTTCAGCATCATCAATAGCC / KJ748363
FaSnRK2.3 / ATGGATCGGGCGGCGGTGACGGT / TTAAATAGCATACACTATTTCCC / KJ748364
FaSnRK2.4 / ATGGAGGAGAGGTATGAGCCAATG / TCAAGCAAAGTCACCACTAAGATC / KJ748365
FaSnRK2.5 / ATGGAGAGGTATGAGATTTTGAAG / TTAGCACACAAAATCACCACTTGT / KJ748366
FaSnRK2.6 / ATGGATCGGTCTATGCTGACAGTTGG / TCAAATTGCATACACTATTTCCCCG / KJ748367
FaSnRK2.7 / ATGGAGAAGTATGAGTTTGTCGAGGAT / TTAAGAGCCTCCAACCGATCGGG / KJ748368
FaSnRK2.8 / ATGGAAAAGTATGAGCTCGTCAAG / TCAGACAACACGTACTTCCC / KJ748369
FaSnRK2.9 / ATGGAGAGGTATGAGATTGTGAAAG / TCAAGCAAAGTCACCACTTGTTTC / KJ748370

Supplemental Table S3: Quantitative PCR primers used in the temporospatial

analysis of FaSnRK2.1 to FaSnRK2.9.

Gene / Forward primers (5’-3’) / Reverse primers (5’-3’)
FaSnRK2.1 / CACAACCCAAATCAACTGTAG / GGATAAGCACCAACCAGCAT
FaSnRK2.2 / CCAGATAGTGTGCGAGTTTCAG / ACTTCCTCCTTCCTTCATTTCC
FaSnRK2.3 / TGATGACGGACAAGCAGAC / TGCCTCAACGACCTATGATT
FaSnRK2.4 / GCTGGAAAACACACTCTTGG / CGGAGCAATGTAAGCAGGT
FaSnRK2.5 / ATGAGATGAACAATCCAGTCCA / TAGCACACAAAATCACCACTTG
FaSnRK2.6 / GCTACACTCGCAACCAAAATC / ACCCCACAAGACCAGACATC
FaSnRK2.7 / TGACTCCTACGCATCTTGCTAT / GAATGACAATGGCTTACACCTG
FaSnRK2.8 / GCATTCACGACCAAAGTCAA / CAAGACCATACATCTGCCAACT
FaSnRK2.9 / TACCCAAACACAGTTACGCATC / GGCAACGAAAATCACCACTT

Supplemental Table S4：Quantitative PCR primers used to analyze the expression of ripening-related marker genes in FaSnRK2.6 OE and RNAi fruits.

Gene / Forward primers (5’-3’) / Reverse primers (5’-3’) / Genbank accession numbers
FaC4H / ACGCTCAACAGAAAGGAGAGAT / TTCGGGGTGGTTCACAA / DQ898278.1
Fa4CL1 / CGTAGACCCTGAAACTGGTG / GTGTAGCCATCCTTCCTTGTC / gene15877
Fa4CL2 / ACGAATCCCTTCCCAGAAA / TCTTTGTCTATGGTCCTCTCAGTC / gene05255
FaCHS / CATACCCCGACTACTACTTTCGT / CGCACATACTGGGATTCTCTT / AY997297.1
FaCHI / AGCGAAAGCCATTGAAAAGT / CATTTGGTGATTGTGTGAAGAG / AB437286.1
FaF3H / CTTTCGTGGTGAATCTTGGAG / TCGCTATGGACAACCTGCT / AB665441.1
FaDFR / ACCCTGAGAACGAAGTGATAAAG / TAAACACCACCCTCCGAACT / AY695813.1
FaUFGT / TAGAGGATGTGTGGAAGATTGGT / CTGTTGTGCGAGTTGTTTTAGTG / AY575056.1
FaANS / CTTGGCTTGGGATTAGAAGAAG / TGAGGGCATTTTGGGTAGTAGT / AY695818.1
FaPE / GGTTTCTACTGGTGCTGGTTTT / CTCGGACTGTATCGTGTTGC / AY324809.1
FaPL / TCAACTCGTCAATGGCAGAC / GAATGCTCGTATCAACCAGAGA / U63550.1
FaPG / GCAAGTAGAGTCGCACAGTTTT / TCAGTATTAGGCTTCCCACCA / DQ45899.1
FaCEL / GCTCTGTTTTGCCTGGACTT / GCGTGGCTTAGATAGTTGGAAT / AF051346.1
FaGAL1 / CAAAAGGGTGGAGCATTCAT / CCAGAGGAGCATCGTAATCATA / AJ278703.1
FaGAL2 / GAGGGAAGGAACGATTTGG / ACAGAGGAAAGCCACCGTAA / AJ278704.1
FaXYL1 / ATGGAAAGCCTACTTGTGCTG / CTGGTGTAATGTTGTTGGTCGT / AY486104.2
FaEXP1 / AGGACGGAGTTGGATTGC / TGAGCGTGAGCGTGAAG / AF163812
FaEXP2 / GTATCGTCCCCGTCTCATTC / AGTAGGAGTGCCCGTTGATT / AF159563
FaEXP3 / TCACTGCCACTAACTTCTGC / TTATGCCTCCTGCTCTCCT / AF226700
FaEXP4 / TACAGCCAGGGGTATGGAAC / GTTAGGGAGGGCATTGTTTG / AF226701
FaEXP5 / CTTCTTACTCTGACCCCATTGTTT / TGTGCTTAGTGCTGCTGTGTT / AF226702
FaQR / CACTGACTCTCCCCTACCTACAAT / ATACACTTCATCCCCCACCTTA / AY048861.1
FaOMT / CACCAAGGGAGTTGTCCAT / GTTGAAAGCATCACAGCAGAC / AF220491.1
FaACTIN / GCCAACCGTGAGAAGATG / TCCAGAGTCAAGAACAATACCAG / AB116565.1

Supplemental Table S5: Primers used to amplify FaSnRK2.6 and FaABI1 in yeast two-hybrid assays

Gene / Forward primers(5’-3’) / Reverse primers(5’-3’)
Y2H-AD-FaABI1 / CATATGATGGAGGAGATGTCACC / GGATCCTCATGTTTTACTTTTAAACTTC
Y2H-BD-FaABI1 / CATATGATGGAGGAGATGTCACC / GTCGACTCATGTTTTACTTTTAAACTTC
Y2H-AD-FaSnRK2.6 / CATATGATGGATCGGTCTATGCTG / CTCGAGTCAAATTGCATACACTATTTCCCCG
Y2H-BD-FaSnRK2.6 / CATATGATGGATCGGTCTATGCTG / GTCGACTCAAATTGCATACACTATTTCCCCG

Supplemental Table S6: Primers used for FaSnRK2.6 and FaABI1 in BiFC assays.

Gene / Forward primers(5’-3’) / Reverse primers(5’-3’)
pCambia::FaABI1-YFPN / GGTACCATGGAGGAGATGTCACC / GGATCCTGTTTTACTTTTAAACTTCCTC
pCambia::FaABI1-YFPC / GGTACCATGGAGGAGATGTCACC / GGATCCTGTTTTACTTTTAAACTTCCTC
pCambia::FaSnRK2.6-YFPN / GAATTCATGGATCGGTCTATGCTG / GTCGACAATTGCATACACTATTTCCCC
pCambia::FaSnRK2.6-YFPC / GAATTCATGGATCGGTCTATGCTG / GTCGACAATTGCATACACTATTTCCCC

Supplemental Table S7: Primers used to amplify proFaSnRK2.6 in the BSP-PCR analysis

Gene / Forward primers(5’-3’) / Reverse primers(5’-3’)
proFaSnRK2.6-1 / GTAGTGGAGAGTTTATATAAATAGAG / CTAAATTAAAATATTCATAATATACCCC
proFaSnRK2.6-2 / GGGGTATATTATGAATATTTTAATTTAG / CCATATACTTAAATTCAAACAAATTAC
proFaSnRK2.6-3 / GTAATTTGTTTGAATTTAAGTATATGG / CTACAAAAATTCCAAATATATTAATAAACAC
proFaSnRK2.6-4 / GTGTTTATTAATATATTTGGAATTTTTGTAG / CAACTATAAAAACAATCTTTCCTAATAAC
proFaSnRK2.6-5 / GTTATTAGGAAAGATTGTTTTTATAGTTG / AATCAATTTTCTAAAATACACATTCACTTC
proFaSnRK2.6-6 / GAAGTGAATGTGTATTTTAGAAAATTGATT / CATAATAACCAACTACAACTACCACC
proFaSnRK2.6-7 / TAGAAATTTGGTGTTTTATTTAG / CTCAAAAATAAACAAAATTACATAAAAACCC
proFaSnRK2.6-8 / GGGTTTTTATGTAATTTTGTTTATTTTTGAG / CTCAAAAATAATAACTCCTATAATC