Supporting Information

Quantitative determination of Butocarboxim in agricultural products: a comparative verification between enzyme-linked immunosorbent assay and electrochemical immunoassay based on biotinylated monoclonal antibody

Qingkui Fang, Limin Wang, Qi Cheng, Yulong Wang, Suyan Wang, Jia Cai,

and Fengquan Liu*

Table S1 Effects of ratio of biotin to anti-Butocarboxim MAb (n=3)

Concentrationa / IC50（ng·mL-1） / Negative (Amax) / Amax/IC50
10* / 362 / 1.09 / 3.02
20* / 295 / 1.03 / 3.48
40* / 414 / 1.22 / 2.95
60* / 502 / 1.16 / 2.3
100* / 432 / 1.04 / 2.4

a Biotin with ratio of molar excess against anti-Butocarboxim MAb concentration.

Figure S1 Effects of pH on bi-ELISA. The ratio of signals (OD value for bi-ELISA in the absence and presence of Butocarboxim in sample) was chosen as the criteria to evaluate the bi-ELISA, the concentration of Butocarboxim was 243 ng·mL-1. And the x axis values are pH values of the solution (n = 3)

Figure S2 Effects of ionic strengths on bi-ELISA. The ratio of signals (the ratio of OD value for bi-ELISA in the absence and presence of Butocarboxim in sample) was chosen as the criteria to evaluate the bi-ELISA, the concentration of Butocarboxim was 243 ng·mL-1. And the x axis values are ionic strengths of the solution (n = 3)

Figure S3 Effects of dilution ratio of ABC on bi-ELISA. The ratio of signals (the ration of OD value for bi-ELISA in the absence and presence of Butocarboxim in sample) was chosen as the criteria to evaluate the bi-ELISA, the concentration of Butocarboxim was 243 ng·mL-1. And the x axis values are dilution ratio of ABC of the solution (n = 3)

Figure S4 Effects of pH on bi-ECIA. The ratio of signals (the ration of SWV response for bi-ECIA in the absence and presence of Butocarboxim in sample) was chosen as the criteria to evaluate the bi-ECIA system, the concentration of Butocarboxim was 243 ng·mL-1. And the x axis values are pH values of the solution (n = 3)

Figure S5 Effects of ionic strengths on bi-ECIA. The ratio of signals (the ration of SWV response for bi-ECIA in the absence and presence of Butocarboxim in sample) was chosen as the criteria to evaluate the bi-ECIA system, the concentration of Butocarboxim was 243 ng·mL-1. And the x axis values are ionic strengths of the solution (n = 3)

Figure S6 Effects of dilution ratio of ABC on bi-ECIA. The ratio of signals (the ratio of SWV response for bi-ECIA in the absence and presence of Butocarboxim in sample) was chosen as the criteria to evaluate the bi-ECIA system, the concentration of Butocarboxim was 243 ng·mL-1. And the x axis values are dilution ratio of ABC of the solution (n = 3)

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