Supplementarytable3homologous Analysis Oftranscript-Derived Fragments (Tdfs) Expressed

SupplementaryTable3Homologous Analysis oftranscript-derived fragments (TDFs) expressed during the chilling stress in maize

Primer comb. / TDFs / Length
(bp) / Homology / Protein / E-value
Metabolism (13)
M1/E3 / T2 / 204 / DQ490951 / Genotype CMS-S mitochondrion
(Zea mays) / 4e-85
M1/E5 / T4 / 312 / EU969652 / Lipid binding protein (Zea mays) / 1e-19
M1/E6 / T5 / 310 / AY941130 / tRNA-Gly (trnG) gene
(Paspalum rufum) / 2e-54
M6/E1 / T12 / 181 / EU976407 / Peptidyl-prolyl isomerase FKBP12
(Zea mays) / 9e-74
M4/E1 / T17 / 265 / NM_001111466 / Dihydrolipoamide S-acetyltransferase
(Zea mays) / 5e-37
M8/E4 / T34 / 125 / EU973416 / Appr-1-p processing enzyme family protein (Zea mays) / 8e-41
M2/E3 / T36 / 138 / DQ645539 / Male-fertile NA mitochondrion
(Zea mays) / 1e-51
M9/E4 / T40 / 192 / AY591012 / Alcohol dehydrogenase 1 (adh1)
(Zea mays) / 2e-12
M9/E8 / T46 / 322 / NM_001155533 / Aspartate aminotransferase(Zea mays) / 2e-32
M14/E1 / T66 / 188 / DQ490951 / CMS-S mitochondrion (Zea mays) / 4e-85
M7/P10 / P26 / 296 / EU974467 / Cysteine synthase (Zea mays) / 1e-55
M10/P10 / P28 / 356 / EU973939 / Hydroxymethylglutary-CoA synthase (Zea mays) / 3e-09
M4/P11 / P30 / 245 / NM_001111853 / Sourose synthase 1 (sus1) (Zea mays) / 1e-49
Photosynthesis and Energy (10)
M4/E5 / T21 / 242 / EU956079 / NADH dehydrogenase 1 alpha subcomplex (Zea mays) / 2e-96
M8/E2 / T25 / 329 / EU960715 / HECT domain and RCC1-like domain-contain (Zea mays) / 2e-59
M8/E2 / T30 / 194 / EU071777 / PhotosystemⅡ protein
(Saccharum arundinaceum) / 2e-37
M2/E7 / T37 / 149 / EF115542 / Cultivar BT×623 chloroplast
(Sorghum bicolor) / 8e-55
M11/P2 / P7 / 274 / EU970929 / GTP-ase activating protein for Arf containing protein (Zea mays) / 4e-30
M4/P3 / P14 / 396 / U45855 / Glyceraldehyde-3-phosphate dehydroqenase GAPC2 (gpc2)
(Zea mays) / 5e-170
M7/P8 / P19 / 133 / EU953062 / Tetratricopeptide repeat protein
(Zea mays) / 7e-48
M3/P9 / P22 / 149 / EU953694 / ACR,COG1565 family protein
(Zea mays) / 3e-21
M7/P12 / P34 / 266 / J01421 / Coupling factor beta-subunit and coupling factor epsilon subunit
(Zea mays) / 6e-117
M5/P13 / P35 / 541 / EU278617 / Sucrose phosphate synthase Ⅲ (SPSⅢ)(Saccharum officinarum) / 6e-26
Transport (5)
M1/E8 / T7 / 361 / EU952492 / Protein translocase/ Protein transporter (Zea mays) / 1e-159
M13/E4 / T60 / 321 / EU977106 / Membrane protein (Zea mays) / 1e-59
M13/E7 / T64 / 158 / NM_119429 / Protein transporter (AT4G32760) (Arabidopsis thaliana) / 8e-17
M6/P3 / P15 / 238 / NM_104659 / Nucleoporin family protein (Arabidopsis thaliana) / 4e-11
M2/P8 / P18 / 169 / EU964743 / Transmembrane BAX inhibitor motif-containing protein 4 (Zea mays) / 4e-65
Signal Transduction (11)
M4/E3 / T8 / 227 / EU963740 / Protein phosphatase 2C isoform epsilon (Zea mays) / 1e-79
M9/E4 / T44 / 245 / AY332472 / Transmembrane kinase (PK1)
(Oryza stiva) / 5e-28
M12/E2 / T53 / 444 / NM_179472 / MAP3K (Mitogen-activated protein kinase kinase kinase)
(Arabidopsis thaliana) / 2e-44
M13/E8 / T65 / 156 / NM_001067315 / Phosphatidylinositol 3,5-kinase like protein (Oryza stiva) / 1e-15
M4/P2 / P5 / 235 / EU963889 / Plant-specific domain TIGR01589 family protein (Zea mays) / 3e-82
M1/P9 / P21 / 232 / EU954588 / Calmodulin binding protein (Zea mays) / 1e-98
M4/P9 / P23 / 336 / EU953194 / Phosphatidylinositol 3- and 4-kinase family protein (Zea mays) / 6e-156
M9/P10 / P27 / 245 / NM_102783 / SIT4 phosphatase-associated family protein (Arabidopsis thaliana) / 2e-26
M5/P12 / P31 / 326 / NM_124062 / Transcription factor jumonji (jmj) family protein (Arabidopsis thaliana) / 2e-35
M2/P16 / P40 / 183 / NM_001156479 / bZIP family transcription factor
(Zea mays) / 2e-42
M4/P16 / P41 / 151 / EU956293 / Ocs element-binding factor 1
(Zea mays) / 6e-12
Secondary Metabolism (2)
M8/E3 / T31 / 222 / DQ680849 / Beta-amyrin synthase (Sad1) and cytochrome P450 (Avena striqosa) / 3e-49
M10/E3 / T48 / 313 / NM_001059606 / Cyanogenic beta-glucosidase precursor
(Oryza stiva) / 4e-52
Defense Response (7)
M1/E1 / T1 / 189 / AY093583 / RNase L inhibitor-like protein
(Oryza stiva) / 2e-44
M7/E6 / T14 / 289 / AY248742 / Omega-3 fatty acid desaturase gene(Lycopersicon esculentum) / 4e-28
M8/E2 / T28 / 272 / EU969433 / Avr9 elicitor response protein
(Zea mays) / 4e-94
M10/E4 / T49 / 249 / NM_128071 / CLPB-M/CLPB4/HSP98.7
(HEAT SHOCK PROTEIN)
(Arabidopsis thaliana) / 9e-19
M12/E2 / T54 / 321 / EU972609 / Virulence-related protein Nf314
(Zea mays) / 2e-130
M8/P16 / P42 / 205 / NM_122525 / CLC-D(chloride channel D); anion channel/voltage-gated chloride channel(Arabidopsis thaliana) / 4e-28
M10/P16 / P43 / 132 / AJ006055 / Glutathione reductase(Zea mays) / 3e-16
Transcription (3)
M10/E4 / T50 / 185 / AF334758 / Homeodomain protein JUBEL1(JuBel1) gene
(Hordeum vulgare) / 3e-04
M5/P12 / P32 / 284 / EU954834 / Cleavage and polyadenylation specificity factor 5 (Zea mays) / 3e-108
M1/P16 / P39 / 326 / EU960671 / PHD finger protein (Zea mays) / 1e-125
Cytoskeleton (3)
M4/P1 / P2 / 273 / EU956930 / CSLE6-cellulose synthase-like family E (Zea mays) / 3e-108
M7/P1 / P3 / 355 / EU955677 / Polygalacturonase inhibitor 1 precursor (Zea mays) / 3e-166
M11/P8 / P20 / 165 / EU971319 / Xylogen protein 1 (Zea mays) / 7e-18
Transposable Elements (4)
M8/E2 / T26 / 303 / DQ002408 / Gypsy retrotransposon huck, and copia retrotransposon ji (Zea mays) / 3e-115
M8/E2 / T27 / 357 / DQ002408 / Gypsy retrotransposon huck, and copia retrotransposon ji (Zea mays) / 3e-96
M9/E4 / T42 / 142 / AF466646 / Putative transposase (Z195D10.1) gene (Zea mays) / 2e-37
M9/E4 / T43 / 166 / AF466646 / Putative transposase (Z195D10.1) gene (Zea mays) / 2e-43
Function Unknown Protein, Hypothetical and Unclassified Protein (39)
M1/E4 / T3 / 473 / AK069807 / Japonica Group cDNA clone: J023028014 (Oryza stiva) / 3e-85
M5/E1 / T9 / 372 / EU972289 / Clone 379149 hypothetical protein
(Zea mays) / 5e-69
M5/E5 / T11 / 282 / AK068598 / cDNA clone: J013154H21
(Oryza stiva) / 1e-37
M7/E4 / T13 / 196 / NM_001136771 / Hypothetical protein LOC100191337 (Zea mays) / 2e-69
M3/E1 / T15 / 277 / BT042771 / cDNA clone ZM BFc0038M14 / 5e-124
M3/E7 / T16 / 379 / AK073387 / cDNA clone: J033043K11
(Oryza stiva) / 7e-42
M4/E1 / T19 / 135 / EU971281 / Clone 361278 (Zea mays) / 1e-32
M4/E2 / T20 / 233 / EU956958 / Clone 1578746 UBP25 (Zea mays) / 4e-36
M8/E2 / T29 / 249 / BT068497 / cDNA-clone ZM BFb0234N24
(Zea mays) / 2e-45
M8/E3 / T32 / 212 / BT036788 / cDNA clone ZM BFb0139B06 (Zea mays) / 4e-86
M8/E8 / T39 / 601 / EU955195 / Clone 1516495 (Zea mays) / 2e-64
M9/E4 / T41 / 192 / AC152495 / BAC clone Z486N13 (Zea mays) / 1e-27
M9/E7 / T45 / 131 / BT035407 / cDNA clone ZM BFb0059G19
(Zea mays) / 9e-47
M12/E1 / T51 / 188 / BT054760 / cDNA clone ZM BFb0029H18
(Zea mays) / 2e-37
M12/E3 / T55 / 140 / AY104225 / PCO144800 (Zea mays) / 1e-32
M12/E5 / T57 / 153 / EU970501 / Clone 346648 (Zea mays) / 2e-44
M13/E3 / T59 / 145 / BT063521 / cDNA clone ZM BFc0072I01
(Zea mays) / 3e-54
M13/E4 / T61 / 134 / EZ060292 / Contig 61414 (Zea mays) / 5e-06
M13/E6 / T62 / 113 / EU942947 / Clone 1447319 (Zea mays) / 9e-08
M13/E7 / T63 / 173 / NM_001049640 / Os01q0388500 (Oryza stiva) / 4e-28
M14/E1 / T67 / 113 / EU951533 / Clone 706470 (Zea mays) / 5e-36
M14/E6 / T68 / 120 / BT018925 / Clone Contiq44.F (Zea mays) / 4e-38
M14/E8 / T69 / 288 / BT038120 / Clone ZM BFb0212P08 (Zea mays) / 3e-102
M14/E8 / T70 / 168 / EU942117 / Clone 1475666 (Zea mays) / 1e-59
M14/E8 / T71 / 168 / EU942117 / Clone 1475666 (Zea mays) / 1e-59
M3/P1 / P1 / 297 / EU965182 / Clone 284337 hypothetical protein
(Zea mays) / 1e-125
M13/P1 / P4 / 222 / EU946205 / Clone 300700 (Zea mays) / 4e-93
M4/P2 / P6 / 211 / BT066928 / Clone BFb0108B05 (Zea mays) / 8e-25
M14/P2 / P9 / 402 / AY109385 / Cl857 2 (Zea mays) / 4e-165
M14/P2 / P10 / 291 / BT043321 / Clone ZM BFc0157G11 (Zea mays) / 1e-131
M14/P2 / P11 / 281 / BT060650 / Clone ZM BF0028K20 (Zea mays) / 1e-80
M14/P2 / P12 / 209 / BT060650 / Clone ZM BF0028K20 (Zea mays) / 3e-87
M4/P3 / P13 / 238 / EZ106884 / Contig42542 (Zea mays) / 5e-104
M7/P3 / P16 / 244 / EU957992 / Clone 1639577 (Zea mays) / 9e-108
M14/P3 / P17 / 244 / EU957992 / Clone 1639577 (Zea mays) / 9e-108
M8/P9 / P25 / 203 / BT062638 / Clone ZM BFb0325N15 (Zea mays) / 5e-78
M8/P13 / P36 / 140 / AP008212 / Genomic DNA, chromosome 6
(Oryza stiva) / 7e-17
M10/P13 / P37 / 234 / AC079179 / Chromosome 10 clone OSJNBa0079B05 (Oryza stiva) / 4e-10
M10/P13 / P38 / 234 / AP008216 / Genomic DNA, chromosome 10 (Oryza stiva) / 5e-09
No Significant Similarity (17)
M1/M6 / T6 / 285 / No hit
M5/E3 / T10 / 182 / No hit
M4/E1 / T18 / 539 / No hit
M4/E4 / T22 / 181 / No hit
M8/E1 / T23 / 238 / No hit
M8/E1 / T24 / 238 / No hit
M8/E4 / T33 / 184 / No hit
M2/E3 / T35 / 221 / No hit
M8/E7 / T38 / 204 / No hit
M10/E3 / T47 / 201 / No hit
M12/E1 / T52 / 158 / No hit
M12/E4 / T56 / 230 / No hit
M12/E5 / T58 / 133 / No hit
M11/P2 / P8 / 258 / No hit
M6/P9 / P24 / 178 / No hit
M1/P11 / P29 / 165 / No hit
M5/P12 / P33 / 274 / No hit