(French-American-British) / Cytogenetics / FLT3-ITD / FLT3-TKD / NPM1 / CEBPa / BCR-ABL / PML RARa / FLT3 expression on blasts (MFI) / FLT3 expression on lymphocytes (MFI)
U-12-2363 / Apheresis / 67 / Male / M5b / Insufficient Metaphases (46,XY[4]/nonclonal[2]) / Negative / Negative / Negative / Negative / Negative / Negative / 14,300 / 565
U-13-2090 / BM / 64 / Male / M5b / 46,XY[20] / Positive / Negative / Negative / Negative / Negative / Negative / 8,796 / 412
U-15-0021 / Apheresis / 65 / Male / M2 / 47,XY,+8[3]/46,XY[12]/
nonclonal[5] / Negative / Negative / Negative / Positive / Negative / Negative / 5,574 / 142
U-11-1780 / BM / 50 / Female / M5b / 46,XX[71]/nonclonal[1] / Positive / Negative / Positive / Negative / Negative / Negative / 5,470 / 266
U-14-0269 / Apheresis / 27 / Male / M5a / 46,XY,t(6;11)(q27;q23)[19]/nonclonal[1] / Negative / Negative / Negative / Negative / Negative / Negative / 2,473 / 127
U-14-1295 / Apheresis / 78 / Female / M5b / 50,XX,+6,+8,+10,+13[11]/46,XX,der(2)t(2;3)(p21;p25),der(3)t(3;11)(p21;p15),der(11)t(3;11)(p21;p15)t(2;3)(p21;p25)[2]/
nonclonal w/clonalabnormalities[2]/46,XX[5] / Negative / Positive / Negative / Negative / Negative / Negative / 2,342 / 180
U-14-0465 / Apheresis / 45 / Male / M1 / 46,XY[19]/nonclonal[1] / Positive / Negative / Positive / Negative / Negative / Negative / 452 / 166
U-14-1077 / Apheresis / 57 / Male / M1 / 46,XY,t(9;22)(q34;q11.2)[18]/nonclonal w/clonal[2] / Negative / Negative / Positive / Negative / Negative / Negative / 1,035 / 127
U-14-1886 / Apheresis / 42 / Male / M2 / 46,XY,t(12;22)(p13;q12)[16]/46,XY,add(10)(p11.2),del(11)(p14)[3]/46,XY[1] / Positive / Negative / Negative / Negative / Negative / Negative / 748 / 81.2
U-14-2181 / Apheresis / 72 / Male / No data / negative for CBFB, negative for inv 16 or t(16;16) / Positive / Negative / Positive / Negative / Negative / Negative / 905 / 595
U-13-0429 / BM / 53 / Female / AML arising from MDS / 46,XX[19]/nonclonal[1] / Negative / Negative / Negative / Negative / Negative / Negative / 441 / 174
U-13-0527 / Apheresis / 68 / Female / M1 / 46,XX[20] / Positive / Negative / Positive / Negative / Negative / Negative / 515 / 247
U-13-1047 / BM / 59 / Male / M4 / 46,XX[20] / Positive / Negative / Negative / Positive / Negative / Negative / 1,010 / 184
U-13-1072 / BM / 59 / Male / M4 / 46,XX[20] / Positive / Negative / Negative / Positive / Negative / Negative / 1,851 / 402
U-13-1323 / BM / 75 / Female / AML-MRC / 46,XX[20] / Negative / Negative / Negative / Negative / Negative / Negative / 1,523 / 204
U-13-1395 / BM / 89 / Female / M1 / Insufficient Metaphases (46,XX[1]) / Negative / Negative / Positive / Negative / Negative / Negative / 1,058 / 268
U-13-1901 / BM / 40 / Male / M2 / 46,XY,inv(16)(p13.1q22)[16]/nonclonal w/inv(16)[1]/46,XY[3] / Negative / Negative / Negative / Negative / Negative / Negative / 658 / 174
SupplementaryTable 1: Clinical Information of AML Samples
(French-American-British) / Karyotype/FISH / FLT3-ITD / FLT3-TKD / NPM1 / CEBPa / BCR-ABL / PML RARa / FLT3 expression on blasts (MFI) / FLT3 expression on lymphocytes (MFI)
U-13-2217 / BM / 23 / Female / M2 / 46,XX[20] / Positive / Negative / Positive / Negative / Negative / Negative / 1,059 / 170
U-12-1461 / BM / 71 / Male / M5 / 46,XY,del(18)(p11.2)[3]/46,XY[18] / Positive / Negative / Positive / Negative / Negative / Negative / 772 / 239
U-12-1576 / BM / 82 / Male / M4 / 46,XY[20] / Negative / Negative / Negative / Negative / Negative / Negative / 274 / 179
U-12-1729 / BM / 82 / Female / AML-MRC / 43,XX,del(5)(q23q32),-7,-12,der(16)t(7;16)(q11.2;q22),-17[18]/nonclonal
w/clonal abnormalities[2] / Negative / Negative / Negative / Negative / Negative / Negative / 180 / 187
U-12-1796 / BM / 62 / Male / M4 / 50,XY,+9,+12,del(12)(p11.2p13)x2,+14,+19[15]/46,XY[4]/
nonclonal[1] / Negative / Negative / Negative / Negative / Negative / Negative / 330 / 202
U-12-2232 / BM / 69 / Male / M1 / 46,XY,t(8;21)(q22;q22)[20] / Negative / Negative / Negative / Negative / Negative / Negative / 713 / 191
U-11-0002 / BM / 80 / Male / AML-MRC / 46,XY[19]/nonclonal[1] / Negative / Negative / Negative / Negative / Negative / Negative / 639 / 187
U-11-0203 / BM / 35 / Female / M1 / 46,XX,del(9)(q22q32)[16]/46,XX[3]/nonclonal[1] / Positive / Negative / Negative / Not tested / Negative / Negative / 527 / 232
U-11-0693 / BM / 77 / Female / M2 / 74-89<4n>,XXXX,-8,-9,-9,-12,-17,i(17)(q10),+mar1,+mar2,+dmin[cp18]/46,XX[2]
.ishdmin(amp CMYC) / Negative / Negative / Negative / Negative / Negative / Negative / 303 / 174
U-11-1758 / BM / 41 / Male / M1 / 47,XY,+4[3]/45,XY,-7[2]/46,XY[17].nucish (D7Z1,D7S486)x1[84/230] / Negative / Negative / Negative / Positive / Negative / Negative / 991 / 208
U-15-0020 / BM / 35 / Female / M4 / inv(16)(p13.1q22) or t(16;16)(p13.1;q22); CBFB-MYH11 / Negative / Positive / Negative / Negative / Negative / Negative / 1,189 / 148
U-14-0196 / BM / 42 / Male / M2 / 46,XY,t(8;21)(q22;q22)[20] / Negative / Negative / Negative / Negative / Negative / Negative / 874 / 154
U-15-0114 / BM / 29 / Male / M1 / 46,XY[20] / Negative / Negative / Positive / Negative / Negative / Negative / 420 / 233