Supplementary Table S1 Pairwise similarities of 16S rRNA, gyrB, rpoB and rpoD genes between strain 46-2T and the closely related strains of P. koreensis SG and P. jenssenii SG.
strains / Pairwise similarity (%)16S rRNA / gyrB / rpoB / rpoD / gyrB-rpoB-rpoD / 16s rRNA-
gyrB-rpoB-rpoD
P. baetica a390T / 99.25 / 93.79 / 96.83 / 90.61 / 94.03 / 95.93
P. granadensis F-278,770T / 99.70 / 92.63 / 96.07 / 92.60 / 93.98 / 96.07
P. helmanticensis OHA11T / 99.02 / 94.22 / 93.55 / 90.33 / 92.77 / 95.05
P. jessenii ClP 105274T / 99.02 / 92.77 / 94.32 / 90.75 / 92.77 / 95.05
P. koreensis Ps 9-14T / 99.62 / 95.52 / 97.05 / 93.74 / 95.58 / 97.06
P. mohnii IpA-2T / 98.64 / 91.47 / 93.33 / 91.04 / 92.08 / 94.47
P. moorei RW10T / 98.19 / 92.34 / 93.88 / 90.90 / 92.51 / 94.58
P. moraviensis 1B4T / 99.47 / 93.50 / 95.63 / 93.74 / 94.42 / 96.26
P. reinekei MT1T / 99.55 / 92.92 / 93.77 / 91.32 / 92.77 / 95.24
P. umsongensis Ps 3-10T / 98.95 / 92.63 / 94.43 / 90.04 / 92.55 / 94.89
P. vancouverensis DhA-51T / 99.55 / 92.49 / 94.32 / 90.61 / 92.64 / 95.16
Supplementary Table S2 GenBank accession numbers of the sequences used in this study
Type Strains / 16s rRNA / gyrB / rpoB / rpoDstrain 46-2T / KT321658.1 / KT183428 / KT183429 / KR709086
strain 46-1 / KU321252 / KU350635 / KU350636 / KU350637
P. antarctica LMG 22709T / AJ537601.1 / FN554169 / FN554727 / FN554450
P. baetica a390T / FN646567.1 / FM201278.1 / HE800504. / FN678357.1
P. brenneri DSM 15294T / AF268968.1 / FN554176 / AJ717482 / FN554457
P. cedrina DSM 17516T / AF064461.1 / FN554178 / AJ717424 / FN554459
P. costantinii LMG 22119T / AF374472 / FN554180 / FN554732 / FN554461
P. extremorientalis LMG 19695T / AF405328.1 / FN554182 / FN554733 / FN554464
P. fragi ATCC 4973T / AF094733.1 / FN554184 / AJ717444 / FN554466
P. gessardi CIP 105469T / NR_024928 / FN554186 / AJ717438 / FN554468
P. granadensis F-278,770T / HG764746 / HG764749 / HG764748 / HG764747
P. grimontii CIP 106645T / AF268029.1 / FN554188 / AJ717439 / FN554470
P. helmanticensis OHA11T / HG940537.1 / HG940516.1 / HE800504.1 / HG940517.
P. jessenii CIP 105274T / AF068259.1 / FN554191 / AJ717447 / FN554473
P. koreensis Ps 9-14T / AF468452.1 / FN554194 / FN554737 / FN554476
P. lini CIP 107460T / AY035996.2 / FN554196 / AJ717466 / FN554478
P. lundensis LMG 13517T / AB021395.1 / FN554197 / AJ717428 / FN554479
P. mandelii CIP 105273T / AF058286.1 / FN554200 / AJ717435 / FN554482
P. meridiana CIP 108465T / AJ537602 / FN554203 / FN554740 / FN554485
P. migulae CCUG 43165T / AF074383.1 / FN554204 / AJ717446 / FN554486
P. mohnii IpA-2T / AM293567.1 / AM293561 / FN554741 / FN554487
P. moorei RW10T / AM293566.1 / AM293560 / FN554742 / FN554489
P. moraviensis 1B4T / AY970952.1 / FN554206 / FN554743 / FN554490
P. orientalis DSM 17489T / AF064457.1 / FN554209 / AJ717434 / FN554493
P. palleroniana LMG 23076T / AY091527.1 / FN554213 / FN554747 / FN554497
P. panacis CIP 108524T / NR_043195.1 / FN554214 / FN554748 / FN554498
P. poae LMG 21465T / AJ492829.1 / FN554219 / FN554751 / FN554504
P. proteolytica CIP 108464T / AJ537603.1 / FN554220 / FN554752 / FN554505
P. psycrhophyla DSM 17535T / AB041885.1 / FN554221 / AJ717464 / FN554506
P. reinekei MT1T / AM293565.1 / AM293559 / FN554754 / FN554508
P. rhodesiae LMG 17764T / AF064459.1 / FN554225 / AJ717431 / FN554511
P. salomonii LMG 22120T / AY091528.1 / FN554226 / FN554756 / FN554512
P. trivialis LMG 21464T / AJ492831.1 / FN554230 / FN554762 / FN554515
P. vancouverensis DhA-51T / AJ011507.1 / FN554232 / AJ717473 / FN554517
P. azotoformans LMG 21611T / D84009.1 / AB039411.1 / AJ717458.1 / KC923841.1
P. simiae CCUG 50988T / AJ936933.1 / FN554227.1 / FN554757.1 / FN554513.1
P. tolaasii ATCC 33618T / NR_114481.1 / AB039423.1 / AJ717467.1 / AB039561.1
P. fluorescens ATCC 13525T / D84013.1 / D86016.1 / AJ717451.1 / D86033.1
P. marginalis ATCC 10844T / Z76663.1 / AB039448.1 / AJ717425.1 / AB039575.1
P. veronii LMG 17761T / AF064460.1 / FN554233.1 / AJ717445.1 / FN554518.1
P. libanensis CIP 105460T / AF057645.1 / FN554195.1 / AJ717454.1 / FN554477.1
P. synxantha LMG 2335T / D84025.1 / AB039415.1 / AJ717420.1 / AB039550.1
P. taetrolens LMG 2336T / D84027.1 / AB039412.1 / AJ717423.1 / AB039523.1
P. frederiksbergensis LMG 19851T / AJ249382.1 / AM084676.1 / AJ717465.1 / AM084335.2
P. mucidolens LMG 2223T / D84017.1 / AB039409.1 / AJ717427.1 / AB039546.1
P. umsongensis Ps 3-10T / AF468450.1 / AM293564.1 / FN554763.1 / FN554516.1
Cellvibrio japonicus Ueda 107T / CP000934.1 / CP000934.1 / CP000934.1 / CP000934.1
Supplementary Table S3. The 16S rRNA gene, three concatenated genes (gyrB, rpoB and rpoD) and four concatenated genes (16S rRNA gene, gyrB, rpoB and rpoD) pairwise sequence similarities between strain 46-2T and the closely related strains of the Pseudomonas spp. belonging to the P. jessenii SG and P. koreensis SG. Strains: 1, strain 46-2T; 2, P. baetica a390T; 3, P. granadensis F-278,770T; 4, P. helmanticensis OHA11T; 5, P. jessenii CIP 105274T; 6, P. koreensis Ps 9-14T; 7, P. mohnii IpA-2T; 8, P. moorei RW10T; 9, P. moraviensis 1B4T; 10, P. reinekei MT1T; 11, P. umsongensis Ps 3-10T; 12, P. vancouverensis DhA-51T
16s rRNA
strains / 1 / 2 / 3 / 4 / 5 / 6 / 7 / 8 / 9 / 10 / 111. strain 46-2T
2. P. baetica a390T / 99.2
3. P. granadensis F-278,770T / 99.7 / 99.1
4. P. helmanticensis OHA11T / 99.0 / 99.3 / 99.3
5. P. jessenii CIP 105274T / 99.0 / 99.3 / 98.9 / 98.9
6. P. koreensis Ps 9-14T / 99.6 / 99.1 / 99.3 / 98.7 / 99.2
7. P. mohnii IpA-2T / 98.6 / 98.9 / 98.5 / 98.5 / 99.0 / 98.9
8. P. moorei RW10T / 98.1 / 98.4 / 98.1 / 98.0 / 98.5 / 98.2 / 98.9
9. P. moraviensis 1B4T / 99.4 / 99.1 / 99.1 / 98.6 / 98.9 / 99.3 / 98.5 / 98.1
10. P. reinekei MT1T / 99.5 / 99.3 / 99.4 / 98.7 / 99.3 / 99.6 / 98.9 / 98.4 / 99.3
11. P. umsongensis Ps 3-10T / 98.9 / 99.6 / 98.8 / 99.2 / 99.3 / 99.1 / 99.1 / 98.3 / 98.8 / 99.2
12. P. vancouverensis DhA-51T / 99.5 / 99.3 / 99.4 / 98.7 / 99.4 / 99.6 / 99.0 / 98.6 / 99.3 / 99.8 / 99.2
Three concatenated gene (gyrB, rpoB and rpoD)
strains / 1 / 2 / 3 / 4 / 5 / 6 / 7 / 8 / 9 / 10 / 111. strain 46-2T
2. P. baetica a390T / 94.0
3. P. granadensis F-278,770T / 93.9 / 93.5
4. P. helmanticensis OHA11T / 92.7 / 94.2 / 93.2
5. P. jessenii CIP 105274T / 92.7 / 92.4 / 92.2 / 92.8
6. P. koreensis Ps 9-14T / 95.5 / 94.8 / 94.8 / 93.0 / 92.7
7. P. mohnii IpA-2T / 92.0 / 91.5 / 91.3 / 92.2 / 94.4 / 91.9
8. P. moorei RW10T / 92.5 / 91.5 / 91.5 / 91.7 / 94.2 / 91.9 / 96.7
9. P. moraviensis 1B4T / 94.4 / 93.8 / 95.2 / 93.6 / 92.3 / 95.1 / 91.6 / 91.4
10. P. reinekei MT1T / 92.7 / 92.2 / 92.2 / 92.9 / 94.8 / 93.2 / 94.4 / 94.5 / 92.4
11. P. umsongensis Ps 3-10T / 92.5 / 92.3 / 91.8 / 92.2 / 95.0 / 92.2 / 94.5 / 94.5 / 91.6 / 94.7
12. P. vancouverensis DhA-51T / 92.6 / 92.2 / 91.6 / 92.5 / 95.6 / 92.4 / 93.8 / 93.8 / 92.4 / 94.3 / 94.9
Four concatenated gene (16s rRNA, gyrB, rpoB and rpoD)
strains / 1 / 2 / 3 / 4 / 5 / 6 / 7 / 8 / 9 / 10 / 111. strain 46-2T
2. P. baetica a390T / 95.9
3. P. granadensis F-278,770T / 96.0 / 95.6
4. P. helmanticensis OHA11T / 95.0 / 96.1 / 95.4
5. P. jessenii CIP 105274T / 95.0 / 94.9 / 94.6 / 95.0
6. P. koreensis Ps 9-14T / 97.0 / 96.3 / 96.4 / 95.1 / 95.1
7. P. mohnii IpA-2T / 94.4 / 94.2 / 93.9 / 94.5 / 96.1 / 94.5
8. P. moorei RW10T / 94.5 / 94.0 / 93.9 / 94.0 / 95.8 / 94.2 / 97.5
9. P. moraviensis 1B4T / 96.2 / 95.7 / 96.7 / 95.4 / 94.7 / 96.7 / 94.2 / 93.8
10. P. reinekei MT1T / 95.2 / 94.8 / 94.9 / 95.1 / 96.4 / 95.5 / 96.0 / 95.9 / 94.9
11. P. umsongensis Ps 3-10T / 94.8 / 95.0 / 94.3 / 94.8 / 96.6 / 94.7 / 96.2 / 95.9 / 94.3 / 96.3
12. P. vancouverensis DhA-51T / 95.1 / 94.8 / 94.5 / 94.8 / 97.0 / 95.0 / 95.7 / 95.5 / 94.9 / 96.3 / 96.5
Supplementary Table S4 Results of DNA–DNA hybridization between the isolates (strains 46-2T and 46-1) and closely related type strains of Pseudomonas species
DNA–DNA relatedness (%) with:strain / 1 / 2 / 3 / 4 / 5 / 6 / 7 / 8 / 9 / 10
1 / ∙ / 93.5±2.4 / 35.5±2.2 / 38.7±2.5 / 27.4±0.9 / 44.7±2.9 / 24.4±3.4 / 31.8±2.3 / 29.6±1.3 / 31.4±2.3
2 / 96.5±1.2 / ∙ / 38.5±3.3 / 40.7±1.5 / 31.4±1.2 / 45.6±1.8 / 24.1±2.9 / 30.9±1.7 / 31.8±2.1 / 35.4±3.3
3 / 39.3±1.4 / 40.3±1.7 / ∙ / 48.3±3.5 / 43.1±2.7 / 41.4±1.6 / 33.4±2.5 / 42.9±2.9 / 48.1±1.8 / 27.1±1.8
4 / 41.5±1.9 / 43.2±0.9 / 49.5±2.2 / ∙ / 44.2±2.9 / 26.7±2.5 / 47.0±2.5 / 22.8±4.0 / 27.1±2.0 / 40.2±3.4
5 / 33.2±1.3 / 35.5±2.3 / 45.3±1.7 / 45.5±3.4 / ∙ / 39.4±3.0 / 34.9±2.3 / 39.3±1.8 / 39.0±1.3 / 31.3±1.3
6 / 45.1±2.5 / 45.4±1.9 / 47.8±2.5 / 46.2±3.1 / 28.0±1.8 / ∙ / 23.6±2.1 / 34.2±1.2 / 24.1±2.0 / 28.9±0.9
7 / 37.7±1.7 / 36.9±2.1 / 35.4±1.9 / 24.7±2.1 / 39.1±0.8 / 38.0±0.9 / ∙ / 36.8±0.9 / 43.0±1.0 / 29.8±1.3
8 / 37.4±4.4 / 38.4±3.1 / 44.4±3.7 / 49.1±2.0 / 33.0±3.0 / 29.0±2.4 / 26.1±0.8 / ∙ / 26.2±3.5 / 32.7±4.1
9 / 38.5±2.4 / 38.3±2.2 / 50.2±2.8 / 28.9±3.0 / 33.7±2.9 / 33.2±2.9 / 29.8±2.3 / 31.9±4.9 / ∙ / 29.1±2.8
10 / 35.6±1.9 / 36.1±2.3 / 28.7±3.8 / 39.2±3.8 / 45.6±3.2 / 45.1±1.4 / 34.9±2.3 / 43.1±1.3 / 43.4±1.2 / ∙
Strains: 1 46-2T; 2 46-1; 3 P. baetica DSM 26532T; 4 P. granadensis DSM 28040T; 5 P. jessenii ATCC 700870T; 6 P. koreensis JCM 14769T; 7 P. mohnii DSM 18327T; 8 P. moraviensis DSM 16007T; 9 P. reinekei DSM 18361T; 10 P. vancouverensis DSM 17555T
Supplementary Table S5. 16S rRNA gene signature nucleotide (red number) which can differentiate P. jessenii SG from P. koreensis SG and the unique nucleotides (blue numbers) of strains 46-1 and 46-2T which differentiate the isolates from P. koreensis Ps 9-14T and P. moraviensis RW10T.
Signature nucleotide in:P. koreensis SG / P. jessenii SG
E. coli
ATCC 11775T
position
(NZ_KK583188.1) / strains 46-1 and 46-2T / P. koreensis Ps 9-14T / P. moraviensis 1B4T / P. granadensis F-278,770T / P. baetica a390T / P. helmanticensis OHA11T / P. reinekei MT1T / P. vancouverensis DhA-51T / P. jessenii CIP 105274T / P. umsongensis Ps 3-10T / P. mohnii IpA-2T / P. moorei RW10T
158 / T / T / T / T / T / T / T / T / T / C / C / T
167 / A / A / A / A / A / A / A / A / A / G / G / A
458 / T / T / T / T / C / C / T / T / N / C / Y / Y
459 / T / T / T / T / A / A / T / T / N / A / W / W
463 / G / G / G / G / A / A / G / G / G / A / R / R
464 / A / A / A / A / A / C / A / A / N / A / M / M
472 / T / T / T / T / T / G / T / T / T / T / K / K
473 / C / C / A / C / T / T / C / C / N / T / Y / Y
474 / T / T / A / T / T / T / T / T / T / T / T / T
475 / G / G / T / G / G / G / G / G / G / G / G / G
476 / C / C / G / C / C / C / C / C / C / C / C / C
477 / A / A / C / A / T / T / A / A / N / T / W / W
478 / A / A / A / A / G / G / A / A / G / G / R / R
593 / C / T / T / C / T / C / T / T / T / T / T / T
612 / T / T / T / T / T / T / T / G / G / T / G / G
639 / C / C / C / T / T / T / T / T / T / T / T / T
648 / G / G / G / T / A / T / A / A / A / A / A / A
650 / G / A / A / G / A / G / A / A / A / A / A / A
1142 / T / A / T / T / T / A / T / T / A / A / W / T
1258 / A / G / A / A / A / A / G / G / G / G / G / G
1293 / T / C / T / T / T / T / T / T / T / T / C / T
1361 / C / C / C / T / C / T / C / C / C / C / C / C
1375 / G / G / G / A / G / A / G / G / G / G / G / G